Strain identifier

BacDive ID: 1634

Type strain: Yes

Species: Bartonella alsatica

Strain history: CIP <- 1998, R. Heller, ULP, Strasbourg, France: strain IBS 382

NCBI tax ID(s): 1094551 (strain), 52764 (species)

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General

@ref: 15703

BacDive-ID: 1634

DSM-Number: 21432

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, Gram-negative, rod-shaped, colony-forming, human pathogen

description: Bartonella alsatica DSM 21432 is a microaerophile, Gram-negative, rod-shaped human pathogen that forms circular colonies and was isolated from blood of wild rabbit.

NCBI tax id

NCBI tax idMatching level
1094551strain
52764species

strain history

@refhistory
15703<- CIP {2008} <- R. Heller, ULP, Strasbourg, France; IBS 382
385531998, R. Heller, ULP, Strasbourg, France: strain IBS 382
116264CIP <- 1998, R. Heller, ULP, Strasbourg, France: strain IBS 382

doi: 10.13145/bacdive1634.20240916.9.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Hyphomicrobiales
  • family: Bartonellaceae
  • genus: Bartonella
  • species: Bartonella alsatica
  • full scientific name: Bartonella alsatica Heller et al. 1999

@ref: 15703

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Rhizobiales

family: Bartonellaceae

genus: Bartonella

species: Bartonella alsatica

full scientific name: Bartonella alsatica Heller et al. 1999

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
23143negative1-2 µm0.5-0.8 µmrod-shaped
116264negativerod-shapedno
69480negative98.783

colony morphology

@refcolony sizecolony colorcolony shapeincubation periodmedium used
23143>1.0 mmwhitecircular10 daysblood agar
116264

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
15703COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
23143blood agaryes
38553MEDIUM 45 - for Columbia agar with sheep bloodyesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Sheepblood (100.000 ml)
116264CIP Medium 45yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=45

culture temp

@refgrowthtypetemperature
15703positivegrowth37
38553positivegrowth37
116264positivegrowth37-41
116264nogrowth10
116264nogrowth15
116264nogrowth45

Physiology and metabolism

oxygen tolerance

  • @ref: 15703
  • oxygen tolerance: microaerophile

spore formation

  • @ref: 69480
  • spore formation: no
  • confidence: 93.93

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2314327082trehalose-builds acid from
23143903434-nitrophenyl N-acetyl-beta-D-glucosaminide-hydrolysis
231433122bis-4-nitrophenyl phosphate-hydrolysis
2314329016arginine+other
2314315428glycine+other
2314325017leucine+other
2314325094lysine+other
2314316811methionine+other
2314326271proline+other
2314327897tryptophan+other
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose-builds acid from
6837132528turanose-builds acid from
6837128066gentiobiose-builds acid from
6837117151xylitol-builds acid from
6837128087glycogen-builds acid from
6837128017starch-builds acid from
6837116634raffinose-builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837127082trehalose-builds acid from
6837117992sucrose-builds acid from
6837128053melibiose-builds acid from
6837117716lactose-builds acid from
6837117306maltose-builds acid from
6837117057cellobiose-builds acid from
6837117814salicin-builds acid from
683714853esculin-builds acid from
6837118305arbutin-builds acid from
6837127613amygdalin-builds acid from
6837159640N-acetylglucosamine-builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837116899D-mannitol-builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose-builds acid from
6837117266L-sorbose-builds acid from
6837116024D-mannose-builds acid from
6837115824D-fructose-builds acid from
6837117634D-glucose-builds acid from
6837112936D-galactose-builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose-builds acid from
6837116988D-ribose-builds acid from
6837130849L-arabinose-builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
6837117754glycerol-builds acid from
1162644853esculin-hydrolysis
11626417632nitrate-reduction
11626416301nitrite-reduction

metabolite production

@refChebi-IDmetaboliteproduction
2314315688acetoinno
11626435581indoleno

metabolite tests

  • @ref: 23143
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: -

enzymes

@refvalueactivityec
23143catalase-1.11.1.6
23143cytochrome oxidase-1.9.3.1
23143pyrazinamidase-3.5.1.B15
23143urease-3.5.1.5
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase-3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin+3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)-
68382alkaline phosphatase-3.1.3.1
116264oxidase-
116264beta-galactosidase+3.2.1.23
116264alcohol dehydrogenase-1.1.1.1
116264gelatinase-
116264catalase-1.11.1.6
116264gamma-glutamyltransferase+2.3.2.2
116264lysine decarboxylase-4.1.1.18
116264ornithine decarboxylase-4.1.1.17
116264tryptophan deaminase-
116264urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
116264---+-+--+--+---+----

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
116264-------------------------------------------------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
23143blood of wild rabbitAlsace, the region in the eastern part of France near the Rhine riverFranceFRAEurope
56916Rabbit blood
15703rabbit blood
116264Animal, Rabbit, blood

isolation source categories

Cat1Cat2Cat3
#Host#Mammals#Leporidae (Rabbit/Hare)
#Host Body Product#Fluids#Blood

Safety information

risk assessment

@refpathogenicity humanpathogenicity animalbiosafety levelbiosafety level comment
15703yesyes2Risk group (German classification)
1162642Risk group (French classification)

Sequence information

16S sequences

  • @ref: 15703
  • description: Bartonella alsatica 16S ribosomal RNA gene
  • accession: AJ002139
  • length: 1440
  • database: nuccore
  • NCBI tax ID: 52764

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Bartonella alsatica CIP 105477GCA_013388295completencbi52764
66792Bartonella alsatica IBS 382T/CIP 105477GCA_902728125contigncbi52764
66792Bartonella alsatica strain CIP 10547752764.3completepatric52764
66792Bartonella alsatica strain IBS 382T/CIP 10547752764.4wgspatric52764

Genome-based predictions

predictions

@reftraitmodeldescriptionpredictionconfidencetraining_data
69480gram-positivegram-positivePositive reaction to Gram-stainingno98.783yes
69480anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)no88.972no
69480aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)no83.782yes
69480spore-formingspore-formingAbility to form endo- or exosporesno93.93no
69480thermophilicthermophileAbility to grow at temperatures above or equal to 45°Cno94.051yes
69480flagellatedmotile2+Ability to perform flagellated movementno88.877no

External links

@ref: 15703

culture collection no.: DSM 21432, CCUG 45774, CIP 105477, IBS 382

straininfo link

  • @ref: 71281
  • straininfo: 67561

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny10028274Bartonella alsatica sp. nov., a new Bartonella species isolated from the blood of wild rabbits.Heller R, Kubina M, Mariet P, Riegel P, Delacour G, Dehio C, Lamarque F, Kasten R, Boulouis HJ, Monteil H, Chomel B, Piemont YInt J Syst Bacteriol10.1099/00207713-49-1-2831999Animals, Bacteremia/*microbiology, Bartonella/genetics/*isolation & purification, Base Sequence, DNA, Bacterial/analysis, Molecular Sequence Data, Nucleic Acid Hybridization, RNA, Ribosomal, 16S/genetics, Rabbits/*microbiologyGenetics
Genetics32816983Complete Genome Sequence of Bartonella alsatica Strain IBS 382 (CIP 105477).Thibau A, Schultze TG, Ballhorn W, Kempf VAJMicrobiol Resour Announc10.1128/MRA.00769-202020

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
15703Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 21432)https://www.dsmz.de/collection/catalogue/details/culture/DSM-21432
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
23143Rémy Heller, Maryline Kubina, Philippe Mariet, Philippe Riegel, Gilles Delacour, Christoph Dehio, Francois Lamarque, Rick Kasten, Henri-Jean Boulouis, Henri Monteil, Bruno Chomel, Yves Piémont10.1099/00207713-49-1-283Bartonella alsatica sp. nov., a new Bartonella species isolated from the blood of wild rabbitsIJSEM 49: 283-288 199910028274
38553Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/17501
56916Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 45774)https://www.ccug.se/strain?id=45774
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
71281Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID67561.1StrainInfo: A central database for resolving microbial strain identifiers
116264Curators of the CIPCollection of Institut Pasteur (CIP 105477)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105477