Strain identifier

BacDive ID: 16325

Type strain: Yes

Species: Streptomyces kunmingensis

Strain history: IFO 14463 <-- IMRU <-- J. Ruan 80-3024.

NCBI tax ID(s): 68225 (species)

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General

@ref: 10464

BacDive-ID: 16325

DSM-Number: 41681

keywords: 16S sequence, Bacteria, spore-forming, mesophilic

description: Streptomyces kunmingensis DSM 41681 is a spore-forming, mesophilic bacterium that builds an aerial mycelium and was isolated from soil.

NCBI tax id

  • NCBI tax id: 68225
  • Matching level: species

strain history

@refhistory
10464<- JCM <- IFO <- IMRU <- J. Ruan, 80-3024
67770IFO 14463 <-- IMRU <-- J. Ruan 80-3024.

doi: 10.13145/bacdive16325.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces kunmingensis
  • full scientific name: Streptomyces kunmingensis (Ruan et al. 1985) Goodfellow et al. 1986

  • synonyms

    • @ref: 20215
    • synonym: Chainia kunmingensis

@ref: 10464

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces kunmingensis

full scientific name: Streptomyces kunmingensis (Ruan et al. 1985) Goodfellow et al. 1986

type strain: yes

Morphology

colony morphology

@refcolony colorincubation periodmedium used
19522Brown10-14 daysISP 2
19522Beige10-14 daysISP 3
19522Red10-14 daysISP 4
19522Brown10-14 daysISP 5
19522Beige10-14 daysISP 6
19522Brown10-14 daysISP 7

multicellular morphology

@refforms multicellular complexcomplex namecomplex colormedium name
19522yesAerial MyceliumWhiteISP 2
19522yesAerial MyceliumBrownISP 3
19522yesAerial MyceliumWhiteISP 4
19522yesAerial MyceliumWhiteISP 5
19522noISP 6
19522yesAerial MyceliumWhiteISP 7

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
10464GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
19522ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19522ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19522ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19522ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19522ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19522ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes

culture temp

@refgrowthtypetemperaturerange
10464positivegrowth28mesophilic
19522positiveoptimum28mesophilic
67770positivegrowth28mesophilic

Physiology and metabolism

spore formation

  • @ref: 19522
  • spore description: Formation of spore chains: spirales, spore surface: smooth
  • type of spore: spore
  • spore formation: yes

halophily

  • @ref: 19522
  • salt: NaCl
  • growth: positive
  • tested relation: maximum
  • concentration: 5 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1952262968cellulose-
1952216634raffinose-
1952226546rhamnose+
1952228757fructose+
1952229864mannitol+
1952217268myo-inositol-
1952218222xylose-
1952217992sucrose-
1952222599arabinose+
1952217234glucose+
683685291gelatin+hydrolysis
6836827897tryptophan-energy source
6836816199urea+hydrolysis
6836816947citrate+assimilation
6836818257ornithine+degradation
6836825094lysine+degradation
6836829016arginine+hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
6836815688acetoinyes
6836835581indoleno
6836816136hydrogen sulfideno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
6836815688acetoin+
6836835581indole-

enzymes

@refvalueactivityec
68368gelatinase+
68368tryptophan deaminase-4.1.99.1
68368urease+3.5.1.5
68368ornithine decarboxylase+4.1.1.17
68368lysine decarboxylase+4.1.1.18
68368arginine dihydrolase+3.5.3.6
68368beta-galactosidase+3.2.1.23

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGEL
19522+++++-+--++

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
10464soilKunming, Dagyanlow's LakeChinaCHNAsia
67770Soil

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
104641Risk group (German classification)
195221risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Streptomyces kunmingensis gene for 16S ribosomal RNA, partial sequence, strain: JCM 7473D44441120ena68225
20218Streptomyces kunmingensis gene for 16S rRNA, partial sequence, strain: NBRC 14463AB1845971454ena68225
20218Streptomyces kunmingensis strain NRRL B-16240T 16S ribosomal RNA gene, partial sequenceDQ4425131487ena68225

GC content

  • @ref: 67770
  • GC-content: 71.3
  • method: thermal denaturation, midpoint method (Tm)

External links

@ref: 10464

culture collection no.: DSM 41681, ATCC 35682, IFO 14463, JCM 7473, NBRC 14463, CGMCC 4.1151, NRRL B-16240, VKM Ac-895

straininfo link

  • @ref: 85381
  • straininfo: 41225

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny22389285Streptomyces endophyticus sp. nov., an endophytic actinomycete isolated from Artemisia annua L.Li J, Zhao GZ, Zhu WY, Huang HY, Xu LH, Zhang S, Li WJInt J Syst Evol Microbiol10.1099/ijs.0.035725-02012Artemisia annua/*microbiology, China, DNA, Bacterial/genetics, Fatty Acids/analysis, Molecular Sequence Data, Nucleic Acid Hybridization, *Phylogeny, Plant Roots/*microbiology, RNA, Ribosomal, 16S/genetics, Streptomyces/*classification/genetics/isolation & purification, Vitamin K 2/analogs & derivatives/analysisGenetics
Phylogeny25663029Streptomyces fractus sp. nov., a novel streptomycete isolated from the gut of a South African termite.Rohland J, Meyers PRAntonie Van Leeuwenhoek10.1007/s10482-015-0404-82015Animals, Bacterial Typing Techniques, DNA, Bacterial/genetics, Gastrointestinal Tract/microbiology, Isoptera/*microbiology, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, South Africa, Streptomyces/*classification/genetics/*isolation & purificationGenetics
Phylogeny30026482Streptomyces qaidamensis sp. nov., isolated from sand in the Qaidam Basin, China.Zhang B, Tang S, Chen X, Zhang G, Zhang W, Chen T, Liu G, Li S, Dos Santos LT, Castro HC, Facey P, Hitchings M, Dyson PJ Antibiot (Tokyo)10.1038/s41429-018-0080-92018China, Genome, Bacterial, Phylogeny, *Soil Microbiology, Streptomyces/*classification/*genetics/isolation & purification/ultrastructureGenetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
10464Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 41681)https://www.dsmz.de/collection/catalogue/details/culture/DSM-41681
19522Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM41681.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
85381Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID41225.1StrainInfo: A central database for resolving microbial strain identifiers