Strain identifier

BacDive ID: 16173

Type strain: Yes

Species: Streptomyces thioluteus

Strain Designation: 26-A

Strain history: CIP <- 2003, JCM <- 1983, KCC <- IFO <- SAJ <- ISP <- Y. Okami: strain 26-A

NCBI tax ID(s): 66431 (species)

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General

@ref: 9226

BacDive-ID: 16173

DSM-Number: 40027

keywords: 16S sequence, Bacteria, obligate aerobe, Gram-positive, filament-shaped

description: Streptomyces thioluteus 26-A is an obligate aerobe, Gram-positive, filament-shaped bacterium of the family Streptomycetaceae.

NCBI tax id

  • NCBI tax id: 66431
  • Matching level: species

strain history

@refhistory
9226<- E.B. Shirling, ISP <- Y. Okami, NIHJ
67770KCC S-0087 <-- H. Yonehara <-- Y. Okami 26-A.
67770KCC S-0182 <-- M. Mayama <-- Y. Okami Z-6-25.
123523CIP <- 2003, JCM <- 1983, KCC <- IFO <- SAJ <- ISP <- Y. Okami: strain 26-A

doi: 10.13145/bacdive16173.20240510.9

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces thioluteus
  • full scientific name: Streptomyces thioluteus (Okami 1952) Witt and Stackebrandt 1991

  • synonyms

    @refsynonym
    20215Streptoverticillium thioluteum
    20215Streptomyces thioluteus

@ref: 9226

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces thioluteus

full scientific name: Streptomyces thioluteus (Okami 1952) Witt and Stackebrandt 1991

strain designation: 26-A

type strain: yes

Morphology

cell morphology

  • @ref: 123523
  • gram stain: positive
  • cell shape: filament-shaped
  • motility: no

colony morphology

  • @ref: 123523
  • hemolysis ability: 1

multimedia

  • @ref: 9226
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_40027.jpg
  • caption: Medium 65 28°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
9226GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
37414MEDIUM 57 - for Streptomyces, Nocardioides, Lentzea albidocapillata and Streptoverticillium reticulumyesDistilled water make up to (1000.000 ml);Agar (15.000 g);Glucose (4.000g);Yeast extract (4.000 g);Malt extract (10.000 g);Calcium carbonate (2.000 g)
9226ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)yeshttps://mediadive.dsmz.de/medium/84Name: ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84) Composition: Agar 20.0 g/l Rolled oats 20.0 g/l ZnSO4 x 7 H2O 0.001 g/l MnCl2 x 4 H2O 0.001 g/l FeSO4 x 7 H2O 0.001 g/l Distilled water
123523CIP Medium 57yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=57

culture temp

@refgrowthtypetemperature
9226positivegrowth28
18466positiveoptimum28
37414positivegrowth30
67770positivegrowth28
123523positivegrowth25-37
123523nogrowth10
123523nogrowth41
123523nogrowth45

Physiology and metabolism

tolerance

  • @ref: 18466
  • compound: Lysozyme
  • percentage: 1

oxygen tolerance

  • @ref: 123523
  • oxygen tolerance: obligate aerobe

compound production

@refcompound
9226aureothin
9226aureothricin

halophily

@refsaltgrowthtested relationconcentration
18466NaClpositivemaximum2.5 %
123523NaClpositivegrowth0-2 %
123523NaClnogrowth4 %
123523NaClnogrowth6 %
123523NaClnogrowth8 %
123523NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
683685291gelatin+hydrolysis
6836827897tryptophan-energy source
6836816199urea+hydrolysis
6836816947citrate+assimilation
6836818257ornithine+degradation
6836825094lysine+degradation
6836829016arginine+hydrolysis
12352316947citrate-carbon source
1235234853esculin+hydrolysis
123523606565hippurate+hydrolysis
12352317632nitrate-reduction
12352316301nitrite-reduction
12352317632nitrate-respiration

antibiotic resistance

  • @ref: 123523
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

@refChebi-IDmetaboliteproduction
67770156452aureothricinyes
67770156450thiolutinyes
6836815688acetoinyes
6836835581indoleno
6836816136hydrogen sulfideno
12352335581indoleno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole testmethylred-test
6836815688acetoin+
6836835581indole-
12352315688acetoin-
12352317234glucose-

enzymes

@refvalueactivityec
68368gelatinase+
68368tryptophan deaminase-4.1.99.1
68368urease+3.5.1.5
68368ornithine decarboxylase+4.1.1.17
68368lysine decarboxylase+4.1.1.18
68368arginine dihydrolase+3.5.3.6
68368beta-galactosidase+3.2.1.23
123523oxidase-
123523beta-galactosidase+3.2.1.23
123523alcohol dehydrogenase-1.1.1.1
123523gelatinase+
123523amylase-
123523DNase+
123523caseinase+3.4.21.50
123523catalase+1.11.1.6
123523tween esterase-
123523gamma-glutamyltransferase+2.3.2.2
123523lecithinase+
123523lipase-
123523lysine decarboxylase-4.1.1.18
123523ornithine decarboxylase-4.1.1.17
123523phenylalanine ammonia-lyase-4.3.1.24
123523protease+
123523tryptophan deaminase-
123523urease+3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)+
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase+3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin+3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase+3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
123523-+++++++-+++---+--+-

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGEL
18466+++++-+--++

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
123523+---+----++--------+-----------++--------------------------++-----------++---+------+-+-++++-++--+-

Isolation, sampling and environmental information

isolation

@refcountryorigin.countrycontinentsample typegeographic location
37414JapanJPNAsia
67770JapanJPNAsiaSoilMitaka City, near Tokyo
123523JapanJPNAsiaEnvironment, Soil

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
92261Risk group (German classification)
184661German classification
1235231Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Streptomyces thioluteus strain IFO 13341 16S ribosomal RNA gene, partial sequenceAY9998481419nuccore66431
20218Streptomyces thioluteus gene for 16S ribosomal RNA, partial sequence, strain: JCM 4087D44001120nuccore66431
20218Streptomyces thioluteus 16S rRNA gene, type strain LMG 20253AJ7813601476nuccore66431
20218Streptomyces thioluteus gene for 16S rRNA, partial sequence, strain: NBRC 13341AB1843441479nuccore66431
20218Streptomyces thioluteus gene for 16S rRNA, partial sequence, strain: NBRC 3364AB1847531467nuccore66431

External links

@ref: 9226

culture collection no.: DSM 40027, ATCC 12310, CBS 642.72, DSM 41486, IFO 13341, IFO 3364, ISP 5027, JCM 4087, JCM 4182, NBRC 13341, NBRC 3364, RIA 1302, NIHJ 26A, BCRC 12428, CGMCC 4.1930, CIP 108140, HUT 6071, JCM 4844, NRRL B-1667, VKM Ac-1914, CCRC 12428, KCC S-0844

straininfo link

  • @ref: 85238
  • straininfo: 45509

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny25030519Streptomyces zagrosensis sp. nov., isolated from soil.Mohammadipanah F, Hamedi J, Sproer C, Rohde M, Montero-Calasanz MDC, Klenk HPInt J Syst Evol Microbiol10.1099/ijs.0.064527-02014Bacterial Typing Techniques, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Iran, Molecular Sequence Data, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Rhizosphere, Sequence Analysis, DNA, *Soil Microbiology, Streptomyces/*classification/genetics/isolation & purificationGenetics
Enzymology27018252Identification of an unusual type II thioesterase in the dithiolopyrrolone antibiotics biosynthetic pathway.Zhai Y, Bai S, Liu J, Yang L, Han L, Huang X, He JBiochem Biophys Res Commun10.1016/j.bbrc.2016.03.1052016Anti-Bacterial Agents/chemistry, Bacterial Proteins/*biosynthesis/genetics/metabolism, Biosynthetic Pathways, Catalytic Domain, Cloning, Molecular, Computational Biology, Esterases/genetics/*metabolism, Gene Deletion, Gene Expression Regulation, Bacterial, Multigene Family, Phylogeny, Pyrroles/*chemistry, Streptomyces/*enzymology/genetics, Sulfhydryl Compounds/*chemistry, Thiolester Hydrolases/*biosynthesis/chemistryMetabolism

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
9226Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 40027)https://www.dsmz.de/collection/catalogue/details/culture/DSM-40027
18466Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM40027.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
37414Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/5730
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
68382Automatically annotated from API zym
85238Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID45509.1StrainInfo: A central database for resolving microbial strain identifiers
123523Curators of the CIPCollection of Institut Pasteur (CIP 108140)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20108140