Strain identifier

BacDive ID: 16049

Type strain: Yes

Species: Streptomyces thermoviolaceus

Strain Designation: R-77

Strain history: KCC S-0337 <-- A. Henssen R77.

NCBI tax ID(s): 1952 (species)

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General

@ref: 9547

BacDive-ID: 16049

DSM-Number: 40443

keywords: genome sequence, 16S sequence, Bacteria, spore-forming, thermophilic

description: Streptomyces thermoviolaceus R-77 is a spore-forming, thermophilic bacterium that was isolated from mixed fresh horse and swine manure.

NCBI tax id

  • NCBI tax id: 1952
  • Matching level: species

strain history

@refhistory
9547<- E.B. Shirling, ISP <- A. Henssen, R-77
67770KCC S-0337 <-- A. Henssen R77.

doi: 10.13145/bacdive16049.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces thermoviolaceus
  • full scientific name: Streptomyces thermoviolaceus Henssen 1957 (Approved Lists 1980)

@ref: 9547

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces thermoviolaceus subsp. thermoviolaceus

full scientific name: Streptomyces thermoviolaceus subsp. thermoviolaceus Henssen 1957

strain designation: R-77

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram stain
69480no93.533
69480100positive

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
9547GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
9547ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium84.pdf

culture temp

@refgrowthtypetemperaturerange
9547positivegrowth45thermophilic
18591positiveoptimum28mesophilic
67770positivegrowth50thermophilic

Physiology and metabolism

spore formation

@refspore formationconfidence
69481yes100
69480yes100

halophily

  • @ref: 18591
  • salt: NaCl
  • growth: positive
  • tested relation: maximum
  • concentration: 7.5 %

observation

  • @ref: 67770
  • observation: quinones: MK-9(H6), MK-9(H8)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1859117234glucose+
1859122599arabinose-
1859117992sucrose-
1859118222xylose-
1859117268myo-inositol-
1859129864mannitol-
1859128757fructose-
1859126546rhamnose-
1859116634raffinose-
1859162968cellulose-
683685291gelatin-hydrolysis
6836827897tryptophan-energy source
6836816199urea+hydrolysis
6836816947citrate+assimilation
6836818257ornithine-degradation
6836825094lysine-degradation
6836829016arginine-hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
6836815688acetoinno
6836835581indoleno
6836816136hydrogen sulfideno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
6836815688acetoin-
6836835581indole-

enzymes

@refvalueactivityec
68368gelatinase-
68368tryptophan deaminase-4.1.99.1
68368urease+3.5.1.5
68368ornithine decarboxylase-4.1.1.17
68368lysine decarboxylase-4.1.1.18
68368arginine dihydrolase-3.5.3.6
68368beta-galactosidase-3.2.1.23

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGEL
18591----+-+----

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
9547mixed fresh horse and swine manure
67770Fresh horse-swine mixed manureBerlinGermanyDEUEurope

isolation source categories

Cat1Cat2Cat3
#Host#Mammals#Equidae (Horse)
#Host#Mammals#Suidae (Pig,Swine)
#Host Body Product#Gastrointestinal tract#Feces (Stool)

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
95471Risk group (German classification)
185911German classification

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218S.thermoviolaceus 16S rRNA geneZ680961483ena1952
20218Streptomyces thermoviolaceus subsp. thermoviolaceus gene for 16S ribosomal RNA, partial sequence, strain: JCM 4337D44059121ena66860
20218Streptomyces thermoviolaceus subsp. thermoviolaceus gene for 16S rRNA, partial sequence, strain: NBRC 13387AB1843711454ena66860
20218Streptomyces thermoviolaceus subsp. thermoviolaceus gene for 16S rRNA, partial sequence, strain: NBRC 13905AB1845451479ena66860

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Streptomyces thermoviolaceus subsp. thermoviolaceus JCM 4843GCA_014650975scaffoldncbi66860
66792Streptomyces thermoviolaceus subsp. thermoviolaceus NBRC 13905GCA_012034235contigncbi66860
66792Streptomyces thermoviolaceus subsp. thermoviolaceus strain JCM 484366860.4wgspatric66860
66792Streptomyces thermoviolaceus subsp. thermoviolaceus strain NBRC 1390566860.3wgspatric66860

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
flagellatedno98.084no
gram-positiveyes91.989no
anaerobicno99.034no
aerobicyes90.507no
halophileno89.336no
spore-formingyes94.945no
thermophileyes60.274no
glucose-utilyes88.133no
motileno93.328no
glucose-fermentno89.581no

External links

@ref: 9547

culture collection no.: DSM 40443, ATCC 19283, CBS 278.66, CBS 688.72, IFO 13387, ISP 5443, NBRC 13387, RIA 1348, JCM 4337, BCRC 12493, BCRC 12639, CGMCC 4.1471, HAMBI 1006, HUT 6604, IFO 13905, IMET 43353, JCM 4843, LMG 14943, LMG 19359, NBRC 13905, NCIMB 10076, NRRL B-12374, VKM Ac-1857, VTT E-991428

straininfo link

  • @ref: 85122
  • straininfo: 13993

literature

Pubmed-IDtitleauthorsjournalDOIyearmeshtopictopic2
16903832Carbohydrate-containing polymers of the cell wall of the thermophilic streptomycete Streptomyces thermoviolaceus subsp. thermoviolaceus VKM Ac-1857T.Kozlova YI, Streshinskaya GM, Shashkov AS, Senchenkova SN, Evtushenko LIBiochemistry (Mosc)10.1134/s00062979060701082006Biopolymers/chemistry, Carbohydrates/*chemistry, Cell Wall/*chemistry, Deuterium Oxide, Magnetic Resonance Spectroscopy, Polysaccharides, Bacterial/*chemistry, Streptomyces/*chemistry/cytology, Sugar Phosphates/chemistry
34787542Reclassification of four subspecies in the genus Streptomyces to Streptomyces rubradiris sp. nov., Streptomyces asoensis sp. nov., Streptomyces fructofermentans sp. nov. and Streptomyces ossamyceticus sp. nov.Komaki H, Tamura TInt J Syst Evol Microbiol10.1099/ijsem.0.0050782021Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Streptomyces/classificationPhylogenyTranscriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
9547Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 40443)https://www.dsmz.de/collection/catalogue/details/culture/DSM-40443
18591Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM40443.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
85122Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID13993.1StrainInfo: A central database for resolving microbial strain identifiers