Strain identifier

BacDive ID: 159889

Type strain: Yes

Species: Actinomadura deserti

Strain history: CGMCC 4.7432 <-- J. Jiang; Jiangsu Normal Univ., China; BMP B8004.

NCBI tax ID(s): 2038117 (species)

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General

@ref: 66653

BacDive-ID: 159889

keywords: 16S sequence, Bacteria, aerobe, Gram-positive

description: Actinomadura deserti CGMCC 4.7432 is an aerobe, Gram-positive bacterium that builds a substrate mycelium and was isolated from From soil.

NCBI tax id

  • NCBI tax id: 2038117
  • Matching level: species

strain history

@refhistory
67771<- Chengliang Cao, Jiangsu Normal Univ.
67770CGMCC 4.7432 <-- J. Jiang; Jiangsu Normal Univ., China; BMP B8004.

doi: 10.13145/bacdive159889.20240510.9

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Streptosporangiales
  • family: Thermomonosporaceae
  • genus: Actinomadura
  • species: Actinomadura deserti
  • full scientific name: Actinomadura deserti Cao et al. 2018

@ref: 66653

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Streptosporangiales

family: Thermomonosporaceae

genus: Actinomadura

species: Actinomadura deserti

type strain: yes

Morphology

cell morphology

  • @ref: 66653
  • gram stain: positive

multicellular morphology

  • @ref: 66653
  • forms multicellular complex: yes
  • complex name: substrate mycelium
  • complex color: deep yellowish-brown
  • further description: with mature aerial mycelium that develops into rod-shaped spore chains

Culture and growth conditions

culture medium

@refnamegrowth
66653Czapek's agaryes
66653ISP 2yes
66653ISP 3yes
66653ISP 4yes
66653ISP 5yes
66653Nutrient agar (NA)yes
66653PDAyes

culture temp

@refgrowthtypetemperature
66653positiveoptimum28
66653positivegrowth20-37
67770positivegrowth28
67771positivegrowth28

culture pH

@refabilitytypepHPH range
66653positiveoptimum7
66653positivegrowth6-10alkaliphile

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
66653aerobe
67771aerobe

halophily

@refsaltgrowthtested relationconcentrationhalophily level
66653NaClpositivegrowth0-4 %(w/v)
66653NaClpositiveoptimum0 %(w/v)non-halophilic

observation

  • @ref: 67770
  • observation: quinones: MK-9(H6), MK-9(H8)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6665316634raffinose-carbon source
6665363150D-rhamnose-carbon source
6665315824D-fructose+carbon source
6665316024D-mannose+carbon source
6665316988D-ribose+carbon source
6665317266L-sorbose+carbon source
6665330849L-arabinose+carbon source
6665365327D-xylose+carbon source
666535291gelatin+hydrolysis
6665328017starch+hydrolysis
6665353426tween 80+hydrolysis
66653casein+hydrolysis
66653milk+hydrolysis
6665316467L-arginine-nitrogen source
6665317895L-tyrosine-nitrogen source
6665315971L-histidine+nitrogen source
6665316643L-methionine+nitrogen source
6665317115L-serine+nitrogen source
6665317196L-asparagine+nitrogen source
6665318019L-lysine+nitrogen source

metabolite production

@refChebi-IDmetaboliteproduction
6665316136hydrogen sulfideno
6665317632nitrateno

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    66653C17:0 10-methyl6
    66653C16:1 w7c / C16:1 w6c8
    66653C15:0 anteiso0.1
    66653C13:0 anteiso0.2
    66653C19:0 10-methyl0.3
    66653C13:0 iso 3OH0.5
    66653C14:1 w5c0.6
    66653C12:0 iso0.6
    66653C17:00.7
    66653C14:00.9
    66653C14:0 iso0.9
    66653C17:1 w8c1.2
    66653C16:1 iso G1.9
    66653C18:1 w9c10.8
    66653C18:0 10-methyl13.8
    66653C16:016.3
    66653C16:0 iso26.9
    66653C18:0 iso6.6
  • type of FA analysis: whole cell analysis
  • incubation medium: tryptic soy broth
  • incubation temperature: 28
  • incubation time: 7
  • software version: Sherlock 6.1
  • library/peak naming table: TSBA6
  • system: MIS MIDI
  • method/protocol: Sasser, 1990

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitudeenrichment cultureenrichment culture compositionenrichment culture durationenrichment culture temperatureisolation procedure
67771From soilXinjiangChinaCHNAsia
66653desert road soilXinjiang ProvinceChinaCHNAsia39.833380.9667humic acid vitamin agarsupplemented with nalidixic acid (10mg/l), cycloheximide (20mg/l) and nystatin (20mg/l)21 days28standard dilution plating method
67770Desert soil from Xinjiang ProvinceChinaCHNAsia

isolation source categories

Cat1Cat2Cat3
#Climate#Hot#Arid
#Environmental#Terrestrial#Soil
#Engineered#Built environment#Transportation ways (Roads)

Sequence information

16S sequences

  • @ref: 66653
  • description: Actinomadura deserti 16S ribosomal RNA gene, partial sequence
  • accession: MF972517
  • length: 1513
  • database: nuccore
  • NCBI tax ID: 2038117

GC content

@refGC-contentmethod
6665371.90thermal denaturation, midpoint method (Tm)
6777071.9thermal denaturation, midpoint method (Tm)

External links

@ref: 66653

culture collection no.: CGMCC 4.7432, KCTC 39998, BMP B8004, JCM 33401

literature

  • topic: Phylogeny
  • Pubmed-ID: 30091696
  • title: Actinomadura deserti sp. nov., isolated from desert soil.
  • authors: Cao C, Xu T, Liu J, Cai X, Sun Y, Qin S, Jiang J, Huang Y
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.002922
  • year: 2018
  • mesh: Actinomycetales/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, *Desert Climate, Fatty Acids/chemistry, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/analogs & derivatives/chemistry
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
66653Chengliang Cao, Tangyu Xu, Jinjuan Liu, Xiaorui Cai, Yong Sun, Sheng Qin, Jihong Jiang and Ying HuangActinomadura deserti sp. nov., isolated from desert soil10.1099/ijsem.0.002922IJSEM 68: 2930-2935 201830091696
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc