General

@ref: 66665

BacDive-ID: 159830

DSM-Number: 106554

keywords: 16S sequence, Bacteria, microaerotolerant, spore-forming, mesophilic, Gram-negative, motile, rod-shaped

description: Simulacricoccus ruber MCy10636 is a microaerotolerant, spore-forming, mesophilic bacterium that was isolated from fresh soil sample.

NCBI tax id

• NCBI tax id: 2303410
• Matching level: species

strain history

• @ref: 69032
• history: <- R. Garcia, Helmholtz Institute for Pharmaceutical Research (HIPS), Saarbrücken, Germany; MCy10636

doi: 10.13145/bacdive159830.20230509.8.1

Name and taxonomic classification

LPSN

• @ref: 20215
• description: domain/bacteria
• keyword: phylum/pseudomonadota
• domain: Bacteria
• phylum: Pseudomonadota
• class: Deltaproteobacteria
• order: Myxococcales
• family: Myxococcaceae
• genus: Simulacricoccus
• species: Simulacricoccus ruber
• full scientific name: Simulacricoccus ruber Garcia and Müller 2018

@ref: 66665

domain: Bacteria

phylum: Proteobacteria

class: Deltaproteobacteria

order: Myxococcales

family: Myxococcaceae

genus: Simulacricoccus

species: Simulacricoccus ruber

full scientific name: Simulacricoccus ruber Garcia and Müller 2018

strain designation: MCy10636

type strain: yes

Morphology

cell morphology

• @ref: 66665
• gram stain: negative
• cell length: 3.0-6.0 µm
• cell width: 0.6-0.7 µm
• cell shape: rod-shaped
• motility: yes

colony morphology

• @ref: 66665
• incubation period: 5-7 days
• medium used: tryptone-based agar

multimedia

Culture and growth conditions

culture medium

• @ref: 69032
• name: R2A MEDIUM (DSMZ Medium 830)
• growth: yes
• link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium830.pdf

culture temp

culture pH

Physiology and metabolism

oxygen tolerance

• @ref: 66665
• oxygen tolerance: microaerotolerant

spore formation

• @ref: 66665
• spore description: Myxospores are refractive, small (1.2–1.4 µm diameter), rounded with a dark coat, arranged as a sheath and desiccation-resistant.
• type of spore: myxospore
• spore formation: yes

halophily

observation

• @ref: 66665
• observation: Vegetative cells are phase-dark, thin, flexuous rods

metabolite utilization

enzymes

Isolation, sampling and environmental information

isolation

• @ref: 66665
• sample type: fresh soil sample
• sampling date: 2013
• geographic location: Saarbrücken
• country: Germany
• origin.country: DEU
• continent: Europe
• enrichment culture: MS21 agar
• enrichment culture composition: baited with a filter paper
• enrichment culture duration: 3 days
• isolation procedure: After 3 days of incubation as a tiny swarming colony on MS21 agar baited with a filter paper. After repeated transfers of the swarm colony edge, the strain was purified and isolated.

isolation source categories

• Cat1: #Environmental
• Cat2: #Terrestrial
• Cat3: #Soil

Sequence information

16S sequences

• @ref: 66665
• description: Cystobacterineae bacterium strain MCy10636 16S ribosomal RNA gene, partial sequence
• accession: MH094235
• length: 1526
• database: ena
• NCBI tax ID: 2303410

GC content

• @ref: 66665
• GC-content: 72.9
• method: high performance liquid chromatography (HPLC)

External links

@ref: 66665

culture collection no.: DSM 106554, NCCB 100651

straininfo link

• @ref: 112173
• straininfo: 401452

literature

• topic: Phylogeny
• Pubmed-ID: 30113300
• title: Simulacricoccus ruber gen. nov., sp. nov., a microaerotolerant, non-fruiting, myxospore-forming soil myxobacterium and emended description of the family Myxococcaceae.
• authors: Garcia R, Muller R
• journal: Int J Syst Evol Microbiol
• DOI: 10.1099/ijsem.0.002936
• year: 2018
• mesh: Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Germany, Myxococcales/*classification/genetics/isolation & purification, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology
• topic2: Transcriptome

Reference