Strain identifier

BacDive ID: 159754

Type strain: Yes

Species: Marinobacter fuscus

Strain Designation: NH169-3

Strain history: <- Yue-Hong Wu, Second Institute of Oceanography SOA

NCBI tax ID(s): 2109942 (species)

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General

@ref: 66682

BacDive-ID: 159754

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, rod-shaped, colony-forming

description: Marinobacter fuscus NH169-3 is an aerobe, mesophilic, Gram-negative bacterium that forms circular colonies and was isolated from surface seawater.

NCBI tax id

  • NCBI tax id: 2109942
  • Matching level: species

strain history

  • @ref: 67771
  • history: <- Yue-Hong Wu, Second Institute of Oceanography SOA

doi: 10.13145/bacdive159754.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Alteromonadales
  • family: Alteromonadaceae
  • genus: Marinobacter
  • species: Marinobacter fuscus
  • full scientific name: Marinobacter fuscus Liu et al. 2018

@ref: 66682

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Alteromonadales

family: Alteromonadaceae

genus: Marinobacter

species: Marinobacter fuscus

strain designation: NH169-3

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
66682negative1.5-2 µm0.5-1 µmrod-shapedno
69480yes98.545
69480negative99.993

colony morphology

  • @ref: 66682
  • colony size: 1-2 mm
  • colony color: brown in the central part with a transparent periphery
  • colony shape: circular
  • incubation period: 3 days
  • medium used: Marine agar (MA)

Culture and growth conditions

culture medium

  • @ref: 66682
  • name: Marine agar (MA)
  • growth: yes

culture temp

@refgrowthtypetemperaturerange
66682positiveoptimum37mesophilic
66682positivegrowth15-40
67771positivegrowth37mesophilic

culture pH

@refabilitytypepHPH range
66682positiveoptimum7.5
66682positivegrowth5.5-9alkaliphile

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
66682aerobe
67771aerobe

spore formation

@refspore formationconfidence
66682no
69480no99.987
69481no100

halophily

@refsaltgrowthtested relationconcentration
66682NaClpositivegrowth0-12.5 %(w/v)
66682NaClpositiveoptimum3 %(w/v)
66682Marine saltspositiveoptimum4 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
666826731melezitose-assimilation
6668216411acetic acid-assimilation
6668216634raffinose-assimilation
6668217057cellobiose-assimilation
6668217115L-serine-assimilation
6668217716lactose-assimilation
6668217754glycerol-assimilation
6668217992sucrose-assimilation
6668227082trehalose-assimilation
6668229864mannitol-assimilation
6668229991L-aspartate-assimilation
6668284997sodium gluconate-assimilation
6668291260disodium malate-assimilation
6668216236ethanol+assimilation
6668216977L-alanine+assimilation
6668217234glucose+assimilation
6668217306maltose+assimilation
6668229985L-glutamate+assimilation
6668253258sodium citrate+assimilation
666826731melezitose-builds acid from
6668216024D-mannose-builds acid from
6668216634raffinose-builds acid from
6668216988D-ribose-builds acid from
6668217108D-arabinose-builds acid from
6668217234glucose-builds acid from
6668217268myo-inositol-builds acid from
6668217317D-sorbose-builds acid from
6668217716lactose-builds acid from
6668217992sucrose-builds acid from
6668229864mannitol-builds acid from
6668229987glutamate-builds acid from
6668230911sorbitol-builds acid from
6668263150D-rhamnose-builds acid from
6668215824D-fructose+builds acid from
6668216236ethanol+builds acid from
6668217057cellobiose+builds acid from
6668217306maltose+builds acid from
6668217754glycerol+builds acid from
6668227082trehalose+builds acid from
6668217234glucose-fermentation
666824853esculin-hydrolysis
666825291gelatin-hydrolysis
6668216199urea-hydrolysis
6668228017starch-hydrolysis
6668253426tween 80-hydrolysis
6668253423tween 40+hydrolysis
6668253424tween 20+hydrolysis
6668253425tween 60+hydrolysis
6668217632nitrate-reduction

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitiveis resistantresistance conc.sensitivity conc.
666822676amoxicillinyesyesyes10 µg (disc)
6668228971ampicillinyesyes10 µg (disc)
666823534cephalexinyesyes30 µg (disc)
6668217698chloramphenicolyesyes30 µg (disc)
6668248923erythromycinyesyes10 µg (disc)
6668217833gentamicinyesyes10 µg (disc)
666826104kanamycinyesyes30 µg (disc)
666827507neomycinyesyes30 µg (disc)
6668228368novobiocinyesyes30 µg (disc)
666827660nystatinyesyes100 µg (disc)
6668217334penicillinyesyes10 Unit (disc)
6668228077rifampicinyesyes5 µg (disc)
6668217076streptomycinyesyes10 µg (disc)
6668228001vancomycinyesyes30 µg (disc)

metabolite production

  • @ref: 66682
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
66682acid phosphatase-3.1.3.2
66682alpha-fucosidase-3.2.1.51
66682alpha-galactosidase-3.2.1.22
66682alpha-glucosidase-3.2.1.20
66682alpha-mannosidase-3.2.1.24
66682arginine dihydrolase-3.5.3.6
66682beta-D-fucosidase-3.2.1.38
66682beta-galactosidase-3.2.1.23
66682beta-glucosidase-3.2.1.21
66682beta-glucuronidase-3.2.1.31
66682chymotrypsin-3.4.4.5
66682cystine arylamidase-3.4.11.3
66682lipase (C 14)-
66682alkaline phosphatase+3.1.3.1
66682catalase+1.11.1.6
66682cytochrome oxidase+1.9.3.1
66682esterase (C 4)+
66682esterase Lipase (C 8)+
66682leucine arylamidase+3.4.11.1
66682N-acetyl-beta-glucosaminidase+3.2.1.52
66682naphthol-AS-BI-phosphohydrolase+
66682valine arylamidase+

Isolation, sampling and environmental information

isolation

@refsample typesampling datecountryorigin.countrycontinentenrichment cultureenrichment culture compositionenrichment culture durationisolation proceduregeographic locationlatitudelongitude
66682surface seawater2014ChinaCHNAsianatural seawater agar(15 g l-1 agar in filtered seawater supplemented with 0.05 % peptone (w/v; BD), 0.01 % yeast extract (w/v; BD) and 1.0 % ornithine (w/v)10 daysApproximately 100 µl diluted seawater was spread on natural seawater agar (using the standard dilution-plating method. The plate was incubated at room temperature (approximately 25-28°C) on the vessel until it was transported to the laboratory after a month. After being cultivated for 10 days, several colonies were isolated from the plate.
67771From seawaterChinaCHNAsiaNorthern South China Sea19118

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Marine
#Environmental#Aquatic#Surface water

taxonmaps

  • @ref: 69479
  • File name: preview.99_108853.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_89;96_693;97_808;98_73918;99_108853&stattab=map
  • Last taxonomy: Marinobacter fuscus subclade
  • 16S sequence: MF487850
  • Sequence Identity:
  • Total samples: 94
  • soil counts: 17
  • aquatic counts: 64
  • animal counts: 13

Sequence information

16S sequences

  • @ref: 66682
  • description: Marinobacter sp. strain NH169-3 16S ribosomal RNA gene, partial sequence
  • accession: MF487850
  • length: 1498
  • database: ena
  • NCBI tax ID: 2109942

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Marinobacter fuscus NH169-3GCA_003007675scaffoldncbi2109942
66792Marinobacter fuscus NH169-32855205042draftimg2109942

GC content

  • @ref: 66682
  • GC-content: 57.2
  • method: genome sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
flagellatedyes81.904yes
gram-positiveno98.752no
anaerobicno95.764no
aerobicyes78.003no
halophileyes87.747no
spore-formingno94.806yes
glucose-utilno53.347yes
thermophileno99.29yes
motileyes91.809no
glucose-fermentno87.251no

External links

@ref: 66682

culture collection no.: MCCC 1K03455, KCTC 62226

literature

  • topic: Phylogeny
  • Pubmed-ID: 30095388
  • title: Marinobacter fuscus sp. nov., a marine bacterium of Gammaproteobacteria isolated from surface seawater.
  • authors: Liu Q, Xamxidin M, Sun C, Cheng H, Meng FX, Wu YH, Wang CS, Xu XW
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.002956
  • year: 2018
  • mesh: Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Glycolipids/chemistry, Marinobacter/*classification/genetics/isolation & purification, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Seawater/*microbiology, Sequence Analysis, DNA, Ubiquinone/chemistry
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
66682Qian Liu, Maripat Xamxidin, Cong Sun, Hong Cheng, Fan-Xu Meng, Yue-Hong Wu, Chun-Sheng Wang, Xue-Wei XuMarinobacter fuscus sp. nov., a marine bacterium of Gammaproteobacteria isolated from surface seawater10.1099/ijsem.0.002956IJSEM 68: 3156-3162 201830095388
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1