Strain identifier

BacDive ID: 159737

Type strain: Yes

Species: Mesorhizobium sanjuanii

NCBI tax ID(s): 2037900 (species)

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General

@ref: 66654

BacDive-ID: 159737

keywords: 16S sequence, Bacteria, aerobe, psychrophilic, Gram-negative, colony-forming

description: Mesorhizobium sanjuanii CECT 9305 is an aerobe, psychrophilic, Gram-negative bacterium that forms circular colonies and was isolated from nodules of Lotus tenuis.

NCBI tax id

  • NCBI tax id: 2037900
  • Matching level: species

doi: 10.13145/bacdive159737.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Hyphomicrobiales
  • family: Phyllobacteriaceae
  • genus: Mesorhizobium
  • species: Mesorhizobium sanjuanii
  • full scientific name: Mesorhizobium sanjuanii Sannazzaro et al. 2018

@ref: 66654

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Hyphomicrobiales

family: Phyllobacteriaceae

genus: Mesorhizobium

species: Mesorhizobium sanjuanii

type strain: yes

Morphology

cell morphology

  • @ref: 66654
  • gram stain: negative

colony morphology

  • @ref: 66654
  • colony color: cream
  • colony shape: circular
  • incubation period: 3-5 days
  • medium used: yeast extract–mannitol agar

Culture and growth conditions

culture medium

  • @ref: 66654
  • name: yeast extract-mannitol agar
  • growth: yes

culture temp

@refgrowthtypetemperaturerange
66654nogrowth10psychrophilic
66654positiveoptimum28mesophilic
66654nogrowth37mesophilic

culture pH

  • @ref: 66654
  • ability: positive
  • type: growth
  • pH: 7-10
  • PH range: alkaliphile

Physiology and metabolism

oxygen tolerance

  • @ref: 66654
  • oxygen tolerance: aerobe

spore formation

  • @ref: 66654
  • spore formation: no

halophily

  • @ref: 66654
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 1 %

observation

  • @ref: 66654
  • observation: Generation time ranges between 5-7 h when grown in TY broth at 28°C and 150 r.p.m.

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
666545291gelatin+carbon source
666548391D-gluconate+carbon source
6665413705acetoacetate+carbon source
6665415740formate+carbon source
6665415748D-glucuronate+carbon source
6665415824D-fructose+carbon source
6665415895D-galactonic acid lactone+carbon source
6665416024D-mannose+carbon source
6665416411acetic acid+carbon source
6665416537galactarate+carbon source
6665416651(S)-lactate+carbon source
66654167632-oxobutanoate+carbon source
6665416899D-mannitol+carbon source
6665416977L-alanine+carbon source
6665417057cellobiose+carbon source
6665417268myo-inositol+carbon source
6665417272propionate+carbon source
6665417306maltose+carbon source
6665417309pectin+carbon source
6665417596inosine+carbon source
6665417716lactose+carbon source
6665417754glycerol+carbon source
6665417924D-sorbitol+carbon source
6665417925alpha-D-glucose+carbon source
6665417992sucrose+carbon source
6665418024D-galacturonic acid+carbon source
6665418183L-pyroglutamic acid+carbon source
6665418333D-arabitol+carbon source
6665422599arabinose+carbon source
6665426490quinate+carbon source
6665427082trehalose+carbon source
6665428800N-acetylgalactosamine+carbon source
6665428847D-fucose+carbon source
6665429985L-glutamate+carbon source
6665429990D-aspartate+carbon source
6665429991L-aspartate+carbon source
6665430797(S)-malic acid+carbon source
6665437657methyl D-glucoside+carbon source
6665453258citric acid+carbon source
6665453423tween 40+carbon source
6665459640N-acetylglucosamine+carbon source
6665462345L-rhamnose+carbon source
6665463153N-acetyl-D-mannosamine+carbon source
6665470744glycine-proline+carbon source
6665473706bromosuccinate+carbon source
6665474611methyl (R)-lactate+carbon source
6665478697D-fructose 6-phosphate+carbon source
66654168102-oxoglutarate+carbon source
6665415971L-histidine-growth
6665416523D-serine-growth
6665417115L-serine-growth
6665417128adipate-growth
66654181014-hydroxyphenylacetic acid-growth
6665418183L-pyroglutamic acid-growth
6665423652dextrin-growth
6665427689decanoate-growth
6665432032potassium gluconate-growth
6665451850methyl pyruvate-growth
666544853esculin+hydrolysis
6665417632nitrate-reduction

metabolite production

  • @ref: 66654
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
66654arginine dihydrolase-3.5.3.6
66654beta-galactosidase-3.2.1.23
66654gelatinase-
66654urease+3.5.1.5

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    66654C16:011.1
    66654C12:019.7
    66654C18:1 w7c37.6
    66654C17:0 iso4.1
    66654C19:0 cyclo w8c5.2
    66654C16:0N OH6.9
    66654C18:1 w7c 11-methyl7.5
    66654C16:1 iso H7.9
  • type of FA analysis: whole cell analysis
  • incubation medium: TY extract agar
  • agar/liquid: agar
  • incubation temperature: 28
  • incubation time: 2
  • incubation_oxygen: aerobic
  • library/peak naming table: TSBA 5.0
  • system: MIS MIDI

Isolation, sampling and environmental information

isolation

  • @ref: 66654
  • sample type: nodules of Lotus tenuis
  • host species: Lotus tenuis
  • sampling date: 2004
  • geographic location: Chascomús, Buenos Aires
  • country: Argentina
  • origin.country: ARG
  • continent: Middle and South America
  • latitude: -35.9166
  • longitude: -58

isolation source categories

Cat1Cat2Cat3
#Host#Plants#Herbaceous plants (Grass,Crops)
#Host Body-Site#Plant#Root nodule

Sequence information

16S sequences

  • @ref: 66654
  • description: Mesorhizobium sp. strain BSA136 16S ribosomal RNA gene, partial sequence
  • accession: MF979853
  • length: 1344
  • database: ena
  • NCBI tax ID: 2037900

GC content

  • @ref: 66654
  • GC-content: 62.51
  • method: genome sequence analysis

External links

@ref: 66654

culture collection no.: CECT 9305, LMG 30060, BSA 136

literature

  • topic: Phylogeny
  • Pubmed-ID: 30063199
  • title: Mesorhizobium sanjuanii sp. nov., isolated from nodules of Lotus tenuis in the saline-alkaline lowlands of Flooding Pampa, Argentina.
  • authors: Sannazzaro AI, Torres Tejerizo G, Fontana MF, Cumpa Velasquez LM, Hansen LH, Pistorio M, Estrella MJ
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.002924
  • year: 2018
  • mesh: Argentina, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Lotus/*microbiology, Mesorhizobium/*classification/genetics/isolation & purification, Multilocus Sequence Typing, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Root Nodules, Plant/*microbiology, Sequence Analysis, DNA
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
66654A. I. Sannazzaro, G. Torres Tejerizo, M. F. Fontana, L. M. Cumpa Velasquez, L. H. Hansen, M. Pistorio, M. J. EstrellaMesorhizobium sanjuanii sp. nov., isolated from nodules of Lotus tenuis in the saline-alkaline lowlands of Flooding Pampa, Argentina10.1099/ijsem.0.002924IJSEM 68: 2936-2942 201830063199