Strain identifier

BacDive ID: 159729

Type strain: Yes

Species: Solitalea longa

Strain Designation: HR-AV

Strain history: C. O. Jeon; Chung-Ang Univ., South Korea; HR-AV.

NCBI tax ID(s): 2079460 (species)

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General

@ref: 66637

BacDive-ID: 159729

keywords: genome sequence, 16S sequence, Bacteria, aerobe, psychrophilic, Gram-negative, motile, rod-shaped, colony-forming

description: Solitalea longa HR-AV is an aerobe, psychrophilic, Gram-negative bacterium that forms circular colonies and was isolated from river water sample.

NCBI tax id

  • NCBI tax id: 2079460
  • Matching level: species

strain history

  • @ref: 67770
  • history: C. O. Jeon; Chung-Ang Univ., South Korea; HR-AV.

doi: 10.13145/bacdive159729.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacteroidota
  • domain: Bacteria
  • phylum: Bacteroidota
  • class: Sphingobacteriia
  • order: Sphingobacteriales
  • family: Sphingobacteriaceae
  • genus: Solitalea
  • species: Solitalea longa
  • full scientific name: Solitalea longa Lee and Jeon 2018

@ref: 66637

domain: Bacteria

phylum: Bacteroidetes

class: Sphingobacteriia

order: Sphingobacteriales

family: Sphingobacteriaceae

genus: Solitalea

species: Solitalea longa

strain designation: HR-AV

type strain: yes

Morphology

cell morphology

  • @ref: 66637
  • gram stain: negative
  • cell length: 2.2-12 µm
  • cell width: 0.4-0.5 µm
  • cell shape: rod-shaped
  • motility: yes

colony morphology

  • @ref: 66637
  • colony color: yellow
  • colony shape: circular
  • medium used: R2A medium

Culture and growth conditions

culture medium

  • @ref: 66637
  • name: Reasoner's 2A agar (R2A)
  • growth: yes

culture temp

@refgrowthtypetemperaturerange
66637positiveoptimum20psychrophilic
66637positivegrowth5-30
67770positivegrowth20psychrophilic

culture pH

  • @ref: 66637
  • ability: positive
  • type: growth
  • pH: 7-8

Physiology and metabolism

oxygen tolerance

  • @ref: 66637
  • oxygen tolerance: aerobe

halophily

  • @ref: 66637
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-0.5 %(w/v)

observation

  • @ref: 67770
  • observation: quinones: MK-7

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6663716899D-mannitol-assimilation
6663717128adipate-assimilation
6663718401phenylacetate-assimilation
6663727689decanoate-assimilation
6663730849L-arabinose-assimilation
6663732032potassium gluconate-assimilation
6663753258sodium citrate-assimilation
6663716024D-mannose+assimilation
6663717306maltose+assimilation
6663717634D-glucose+assimilation
6663725115malate+assimilation
6663759640N-acetylglucosamine+assimilation
6663717234glucose-fermentation
6663718186tyrosine-hydrolysis
6663728017starch-hydrolysis
6663753424tween 20-hydrolysis
6663753426tween 80-hydrolysis
66637casein-hydrolysis
666374853esculin+hydrolysis
6663717632nitrate-reduction

metabolite production

  • @ref: 66637
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
66637arginine dihydrolase-3.5.3.6
66637beta-glucuronidase-3.2.1.31
66637catalase-1.11.1.6
66637urease-3.5.1.5
66637acid phosphatase+3.1.3.2
66637alkaline phosphatase+3.1.3.1
66637alpha-chymotrypsin+3.4.21.1
66637alpha-fucosidase+3.2.1.51
66637alpha-galactosidase+3.2.1.22
66637alpha-glucosidase+3.2.1.20
66637alpha-mannosidase+3.2.1.24
66637beta-galactopyranosidase+
66637beta-galactosidase+3.2.1.23
66637beta-glucosidase+3.2.1.21
66637cystine arylamidase+3.4.11.3
66637cytochrome oxidase+1.9.3.1
66637esterase (C 4)+
66637esterase Lipase (C 8)+
66637leucine arylamidase+3.4.11.1
66637lipase (C 14)+
66637N-acetyl-beta-glucosaminidase+3.2.1.52
66637naphthol-AS-BI-phosphohydrolase+
66637trypsin+3.4.21.4
66637valine arylamidase+

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    66637C16:01
    66637C15:0 iso 3OH4
    6663710-methyl C16:0 and/or C17:1 w9c1.1
    66637C15:01.2
    66637C15:0 2OH1.2
    66637C16:0 iso 3OH1.2
    66637C17:1 iso / C17:1 anteiso B1.2
    66637C19:01.3
    66637C15:0 anteiso10.3
    66637C16:1 w7c and/or C16:1 w6c12.7
    66637C14:0 iso2.1
    66637C16:0 iso2.2
    66637C16:0 3OH2.6
    66637C15:0 iso28.9
    66637C17:0 iso 3OH8.1
    66637C16:1 w5c8.5
  • type of FA analysis: whole cell analysis
  • incubation medium: R2A broth
  • agar/liquid: liquid
  • incubation temperature: 20
  • software version: Sherlock 6.2B
  • library/peak naming table: TSBA6
  • system: MIS MIDI
  • cutoff value:

Isolation, sampling and environmental information

isolation

@refsample typesampling dategeographic locationcountryorigin.countrycontinentlatitudelongitudeenrichment cultureenrichment culture durationenrichment culture temperatureisolation procedure
66637river water sample2017-07-18HeukSeok area, Han RiverRepublic of KoreaKORAsia37.5093126.965R2A agar5 days25For the isolation of bacteria, a river water sample collected on July 18, 2017 was serially diluted in phosphate-buffered saline (PBS; 137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 2 mM KH2PO4, pH 7.2) and 100 µl of each dilution was spread on Reasoner's 2A agar (BD) and incubated aerobically at 25°C for 5 days.
67770Freshwater sample from the Heukseok area of the Han RiverRepublic of KoreaKORAsia

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Aquatic
  • Cat3: #River (Creek)

Sequence information

16S sequences

  • @ref: 66637
  • description: Solitalea sp. strain HR-AV 16S ribosomal RNA gene, complete sequence
  • accession: MF685247
  • length: 1523
  • database: ena
  • NCBI tax ID: 2079460

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Solitalea longa HR-AVGCA_002920915contigncbi2079460
66792Solitalea longa HR-AV2832312960draftimg2079460

GC content

  • @ref: 67770
  • GC-content: 38.4
  • method: genome sequence analysis

External links

@ref: 66637

culture collection no.: KACC 19411, JCM 32259

literature

  • topic: Phylogeny
  • Pubmed-ID: 30016230
  • title: Solitalea longa sp. nov., isolated from freshwater and emended description of the genus Solitalea.
  • authors: Lee Y, Jeon CO
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.002903
  • year: 2018
  • mesh: Bacterial Typing Techniques, Bacteroidetes/*classification/genetics/isolation & purification, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Nucleic Acid Hybridization, Phosphatidylethanolamines/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Rivers/*microbiology, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistry
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
66637Yunho Lee, Che Ok JeonSolitalea longa sp. nov., isolated from freshwater and emended description of the genus Solitalea10.1099/ijsem.0.002903IJSEM 68: 2826-2831 201830016230
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/