Strain identifier
BacDive ID: 159665
Type strain: 
Species: Stigonema ocellatum
Strain Designation: 232
Strain history: <- T. Friedl, SAG; SAG 48.90 {1988} <- D. Mollenhauer, EAWAG; {1970}
NCBI tax ID(s): 221294 (strain), 221293 (species)
version 9.1 (current version)
General
@ref: 66515
BacDive-ID: 159665
DSM-Number: 106950
keywords: genome sequence, 16S sequence, Bacteria, Gram-negative
description: Stigonema ocellatum 232 is a Gram-negative bacterium that was isolated from Sphagnum bog.
NCBI tax id
| NCBI tax id | Matching level |
|---|---|
| 221294 | strain |
| 221293 | species |
strain history
- @ref: 66515
- history: <- T. Friedl, SAG; SAG 48.90 {1988} <- D. Mollenhauer, EAWAG; {1970}
doi: 10.13145/bacdive159665.20240916.9.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/cyanobacteriota
- domain: Bacteria
- phylum: Cyanobacteriota
- class: Cyanophyceae
- order: Nostocales
- family: Stigonemataceae
- genus: Stigonema
- species: Stigonema ocellatum
- full scientific name: Stigonema ocellatum Thuret ex Bornet and Flahault 1886
@ref: 66515
domain: Bacteria
phylum: Cyanobacteria
class: Cyanophyceae
order: Nostocales
family: Stigonemataceae
genus: Stigonema
species: Stigonema ocellatum
full scientific name: Stigonema ocellatum
strain designation: 232
type strain: no
Morphology
multimedia
- @ref: 66515
- multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_106950..png
- caption: SAG
- intellectual property rights: Leibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH
Culture and growth conditions
culture medium
- @ref: 66515
- name: Z 45/4 (DSMZ Medium 1727)
- growth: yes
- link: https://mediadive.dsmz.de/medium/1727
- composition: Name: Z45/4 Medium for Cyanobacteria (DSMZ Medium 1727) Composition: KH2PO4 0.041 g/l CaCl2 x 2 H2O 0.037 g/l MgSO4 x 7 H2O 0.025 g/l K2HPO4 0.017 g/l Na-EDTA 0.00372 g/l FeCl3 x 6 H2O 0.0027 g/l H3BO3 0.000186 g/l MnSO4 x H2O 0.0001338 g/l AlK(SO4)2 x 12 H2O 2.844e-05 g/l ZnSO4 x 7 H2O 1.722e-05 g/l (NH4)2Ni(SO4)2 x 6 H2O 1.188e-05 g/l Cd(NO3)2 x 4 H2O 9.24e-06 g/l Co(NO3)2 x 6 H2O 8.76e-06 g/l CuSO4 x 5 H2O 7.5e-06 g/l KBr 7.14e-06 g/l (NH4)6Mo7O24 x 4 H2O 5.28e-06 g/l KJ 4.98e-06 g/l Cr(NO3)3 x 7 H2O 2.22e-06 g/l Na2WO4 x 2 H2O 1.98e-06 g/l VOSO4 x 2 H2O 1.2e-06 g/l Distilled water
culture temp
- @ref: 66515
- growth: positive
- type: growth
- temperature: 17
Isolation, sampling and environmental information
isolation
- @ref: 66515
- sample type: Sphagnum bog
- geographic location: Allgäu
- country: Germany
- origin.country: DEU
- continent: Europe
- latitude: 47.6876
- longitude: 10.2155
isolation source categories
- Cat1: #Environmental
- Cat2: #Terrestrial
- Cat3: #Wetland (Swamp)
Safety information
risk assessment
- @ref: 66515
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 124043
- description: Stigonema ocellatum SAG 48.90 partial 16S rRNA gene, strain SAG 48.90
- accession: AJ544082
- length: 1352
- database: nuccore
- NCBI tax ID: 221294
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Stigonema ocellatum SAG 48.90 = DSM 106950 JP211 | GCA_016903675 | contig | ncbi | 221294 |
| 66792 | Stigonema ocellatum SAG 48.90 = DSM 106950 strain JP211 | 221294.3 | wgs | patric | 221294 |
Genome-based predictions
predictions
| @ref | trait | model | description | prediction | confidence | training_data |
|---|---|---|---|---|---|---|
| 69481 | spore-forming | spore-forming | Ability to form endo- or exospores | no | 76 | no |
| 69480 | gram-positive | gram-positive | Positive reaction to Gram-staining | no | 80.075 | no |
| 69480 | anaerobic | anaerobic | Ability to grow under anoxygenic conditions (including facultative anaerobes) | no | 71.792 | no |
| 69480 | spore-forming | spore-forming | Ability to form endo- or exospores | no | 72.427 | no |
| 69480 | aerobic | aerobic | Ability to grow under oxygenic conditions (including facultative aerobes) | yes | 60.802 | no |
| 69480 | thermophilic | thermophile | Ability to grow at temperatures above or equal to 45°C | no | 92.859 | yes |
| 69480 | flagellated | motile2+ | Ability to perform flagellated movement | no | 63.122 | no |
External links
@ref: 66515
culture collection no.: DSM 106950, SAG 48.90, IAM M-252
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 31280092 | Heterocyte glycolipids indicate polyphyly of stigonematalean cyanobacteria. | Bauersachs T, Miller SR, Gugger M, Mudimu O, Friedl T, Schwark L | Phytochemistry | 10.1016/j.phytochem.2019.112059 | 2019 | Cyanobacteria/*classification/genetics/*metabolism, Glycolipids/*metabolism, *Phylogeny, RNA, Ribosomal, 16S/genetics | Metabolism |
| Genetics | 33803228 | Filling the Gaps in the Cyanobacterial Tree of Life-Metagenome Analysis of Stigonema ocellatum DSM 106950, Chlorogloea purpurea SAG 13.99 and Gomphosphaeria aponina DSM 107014. | Marter P, Huang S, Brinkmann H, Pradella S, Jarek M, Rohde M, Bunk B, Petersen J | Genes (Basel) | 10.3390/genes12030389 | 2021 | Cyanobacteria/*genetics, Genome, Bacterial/genetics, Metagenome/*genetics, Metagenomics/methods, Microbiota/genetics, Phylogeny | Phylogeny |
Reference
| @id | authors | title | doi/url | catalogue |
|---|---|---|---|---|
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |
| 66515 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-106950 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 106950) | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |
| 69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | Predictions based on the model GenomeNet Sporulation v. 1 | 10.21203/rs.3.rs-2527258/v1 | |
| 124043 | Dr. Isabel Schober, Dr. Julia Koblitz | Data extracted from sequence databases, automatically matched based on designation and taxonomy |