Strain identifier

BacDive ID: 159651

Type strain: Yes

Species: Streptomyces triticisoli

Strain Designation: NEAU-DSCPA1-4-4

Strain history: <- J. Zhao; School of Life Science, Harbin, P.R. CHINA; NEAU-DSCPA1-4-4<-C. Han; School of Life Science, Northease Agricultural University

NCBI tax ID(s): 2182797 (species)

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General

@ref: 66501

BacDive-ID: 159651

DSM-Number: 105118

keywords: genome sequence, 16S sequence, Bacteria, aerobe, spore-forming, mesophilic, Gram-positive

description: Streptomyces triticisoli NEAU-DSCPA1-4-4 is an aerobe, spore-forming, mesophilic bacterium that builds a substrate hyphae and aerial mycelium and was isolated from Soil.

NCBI tax id

  • NCBI tax id: 2182797
  • Matching level: species

strain history

  • @ref: 66501
  • history: <- J. Zhao; School of Life Science, Harbin, P.R. CHINA; NEAU-DSCPA1-4-4<-C. Han; School of Life Science, Northease Agricultural University

doi: 10.13145/bacdive159651.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces triticisoli
  • full scientific name: Streptomyces triticisoli Tian et al. 2018

@ref: 66501

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Streptomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces triticisoli

full scientific name: Streptomyces triticisoli Tian et al. 2018

strain designation: NEAU-DSCPA1-4-4

type strain: yes

Morphology

cell morphology

  • @ref: 66710
  • gram stain: positive
  • motility: no

multicellular morphology

  • @ref: 66710
  • forms multicellular complex: yes
  • complex name: substrate hyphae and aerial mycelium
  • medium name: ISP 1, ISP 3

multimedia

  • @ref: 66501
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_105118.jpg
  • caption: Medium 65 28°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
66501GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
66710Bennett's agaryes
66710Czapek's agarno
66710ISP 1yes
66710ISP 2yes
66710ISP 3yes
66710ISP 4yes
66710ISP 5yes
66710ISP 6yes
66710ISP 7yes
66710Nutrient agar (NA)no

culture temp

@refgrowthtypetemperaturerange
66501positivegrowth28mesophilic
66710positiveoptimum28mesophilic
66710positivegrowth10-40

culture pH

@refabilitytypepHPH range
66710positiveoptimum7
66710positivegrowth5-10alkaliphile

Physiology and metabolism

oxygen tolerance

  • @ref: 66710
  • oxygen tolerance: aerobe

spore formation

  • @ref: 66710
  • spore description: long spore chains with warty surfaced spores
  • type of spore: spore
  • spore formation: yes

halophily

  • @ref: 66710
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 6 %(w/v)

observation

@refobservation
66710predominant menaquinones are MK-9(H 8) and MK-9(H 6)
66710lipid profile consists of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol mannoside and three unidentified phospholipids

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6671016136hydrogen sulfide-assimilation
6671016634raffinose-carbon source
6671012936D-galactose+carbon source
6671015824D-fructose+carbon source
6671016024D-mannose+carbon source
6671016899D-mannitol+carbon source
6671016988D-ribose+carbon source
6671017268myo-inositol+carbon source
6671017306maltose+carbon source
6671017634D-glucose+carbon source
6671017924D-sorbitol+carbon source
6671017992sucrose+carbon source
6671030849L-arabinose+carbon source
6671062345L-rhamnose+carbon source
6671065327D-xylose+carbon source
6671053424tween 20-degradation
6671062968cellulose-hydrolysis
667104853esculin+hydrolysis
6671028017starch+hydrolysis
6671053423tween 40+hydrolysis
6671053426tween 80+hydrolysis
6671015428glycine-nitrogen source
6671017203L-proline-nitrogen source
6671017895L-tyrosine-nitrogen source
6671016467L-arginine+nitrogen source
6671016857L-threonine+nitrogen source
6671016919creatine+nitrogen source
6671016977L-alanine+nitrogen source
6671017115L-serine+nitrogen source
6671017196L-asparagine+nitrogen source
6671018050L-glutamine+nitrogen source
6671029985L-glutamate+nitrogen source
6671029991L-aspartate+nitrogen source
6671017632nitrate+reduction

enzymes

@refvalueactivityec
66710gelatinase+
66710urease+3.5.1.5

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    66710C18:012
    66710C17:00.7
    66710C17:1 w7c0.7
    66710C17:0 iso0.7
    66710C16:014.6
    66710C14:0 iso14.9
    66710C15:02.8
    66710C16:0 iso32.6
    66710C16:1 w7c5.5
    66710C17:0 anteiso5.7
    66710C15:0 anteiso9.8
  • type of FA analysis: whole cell analysis
  • incubation medium: GY broth
  • incubation temperature: 28
  • incubation time: 7
  • system: MIS MIDI
  • method/protocol: Xiang et al. 2011

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitudeenrichment cultureenrichment culture compositionenrichment culture durationenrichment culture temperatureisolation procedure
66501SoilZhumadian, Henan province (32° 98' N 114° 02' E)ChinaCHNAsia33.6333114.033
66710rhizosphere soil of wheatZhuMadian, HenanChinaCHNAsia34.4833113.033Gause's synthetic agar no. 1supplemented with cycloheximide (50 mg/L) and nalidixic acid (20 mg/L)21 days28soil sample was air-dried at room temperature for 14 days before isolation. After drying, 5 g soil sample was mixed with 45 ml distilled water and followed by shaking on a rotary shaker at 250 r.p.m., 28 C for 30 min. Then a 200 µl sample of the suspension was spread on a plate of Gause's synthetic agar no. 1

isolation source categories

Cat1Cat2Cat3
#Engineered#Agriculture#Field
#Host#Plants#Herbaceous plants (Grass,Crops)
#Host Body-Site#Plant#Rhizosphere
#Environmental#Terrestrial#Soil

Sequence information

16S sequences

  • @ref: 66710
  • description: Streptomyces sp. strain NEAU DSCPA1-4-4 16S ribosomal RNA gene, partial sequence
  • accession: MF993053
  • length: 1519
  • database: ena
  • NCBI tax ID: 2182797

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Streptomyces triticisoli NEAU-DSCPA1-4-4GCA_003270085contigncbi2182797
66792Streptomyces triticisoli NEAU-DSCPA1-4-42862233620draftimg2182797

GC content

  • @ref: 66501
  • GC-content: 71.3
  • method: thermal denaturation, midpoint method (Tm)

External links

@ref: 66501

culture collection no.: DSM 105118, CCTCC AA 2017025

straininfo link

  • @ref: 112125
  • straininfo: 398093

literature

  • topic: Phylogeny
  • Pubmed-ID: 30141768
  • title: Streptomyces triticisoli sp. nov., a novel actinomycete isolated from rhizosphere soil of wheat (Triticum aestivum L.).
  • authors: Tian Y, Han C, Zhao J, Shi H, Hu J, Jiang S, Han X, Wang X, Xiang W
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.002993
  • year: 2018
  • mesh: Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, *Rhizosphere, Sequence Analysis, DNA, *Soil Microbiology, Streptomyces/*classification/genetics/isolation & purification, Triticum/*microbiology, Vitamin K 2/chemistry
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
66501Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-105118Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 105118)
66710Yuanyuan Tian, Chuanyu Han, Junwei Zhao, Haoran Shi, Jiangmeihui Hu, Shanwen Jiang, Xiaoyang Han, Xiangjing Wang, Wensheng XiangStreptomyces triticisoli sp. nov., a novel actinomycete isolated from rhizosphere soil of wheat (Triticum aestivum L.)10.1099/ijsem.0.002993IJSEM 68: 3327-3332 201830141768
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
112125Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID398093.1