Strain identifier

BacDive ID: 159648

Type strain: Yes

Species: Streptomyces caeni

Strain Designation: HA15955

Strain history: <- H. Huang, Chinese Acad. Tropical Agricult. Sci., Haikou, China; HA15955

NCBI tax ID(s): 2307231 (species)

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General

@ref: 66498

BacDive-ID: 159648

DSM-Number: 105693

keywords: 16S sequence, Bacteria, aerobe, spore-forming, mesophilic, Gram-positive, colony-forming

description: Streptomyces caeni HA15955 is an aerobe, spore-forming, mesophilic bacterium that forms filamentous colonies and was isolated from mangrove mud.

NCBI tax id

  • NCBI tax id: 2307231
  • Matching level: species

strain history

  • @ref: 66498
  • history: <- H. Huang, Chinese Acad. Tropical Agricult. Sci., Haikou, China; HA15955

doi: 10.13145/bacdive159648.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces caeni
  • full scientific name: Streptomyces caeni Huang et al. 2018

@ref: 66498

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Streptomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces caeni

full scientific name: Streptomyces caeni Huang et al. 2018

strain designation: HA15955

type strain: yes

Morphology

cell morphology

  • @ref: 66648
  • gram stain: positive

colony morphology

@refcolony colorcolony shapeincubation periodmedium used
66648greyish withefilamentous14 daysISP 1 agar
66648greyish whitefilamentous14 daysISP 2 agar
66648greyish whitefilamentous14 daysISP 4 agar
66648greyish whitefilamentous14 daysISP 5 agar
66648creamy yellowfilamentous14 daysISP 6 agar
66648greyishfilamentous14 daysISP 7 agar

multimedia

  • @ref: 66498
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_105693.jpg
  • caption: Medium 252 28°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
66498GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
66498ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)yeshttps://mediadive.dsmz.de/medium/84Name: ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84) Composition: Agar 20.0 g/l Rolled oats 20.0 g/l ZnSO4 x 7 H2O 0.001 g/l MnCl2 x 4 H2O 0.001 g/l FeSO4 x 7 H2O 0.001 g/l Distilled water
66498STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252)yeshttps://mediadive.dsmz.de/medium/252Name: STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252) Composition: Agar 14.985 g/l Starch 9.99001 g/l (NH4)2SO4 1.998 g/l CaCO3 1.998 g/l K2HPO4 0.999001 g/l MgSO4 x 7 H2O 0.999001 g/l NaCl 0.999001 g/l FeSO4 x 7 H2O 0.000999001 g/l MnCl2 x 4 H2O 0.000999001 g/l ZnSO4 x 7 H2O 0.000999001 g/l Distilled water
66648ISP 1 agaryes
66648ISP 2 agaryes
66648ISP 4 agaryes
66648ISP 5 agaryes
66648ISP 6 agaryes
66648ISP 7 agaryes

culture temp

@refgrowthtypetemperaturerange
66498positivegrowth28mesophilic
66648positiveoptimum28mesophilic
66648positivegrowth20-37

culture pH

@refabilitytypepHPH range
66648positivegrowth5-10alkaliphile
66648positiveoptimum7-8

Physiology and metabolism

oxygen tolerance

  • @ref: 66648
  • oxygen tolerance: aerobe

spore formation

  • @ref: 66648
  • spore description: cylindrical in shape with smooth surfaces
  • type of spore: spore
  • spore formation: yes

halophily

@refsaltgrowthtested relationconcentration
66648NaClpositivegrowth0-5 %(w/v)
66648NaClpositiveoptimum1 %(w/v)

observation

@refobservation
66648forms creamy yellow to brown substrate mycelium and greyish-white to greyish-yellow aerial hyphae that differentiate into straight to spiral chains.
66648No diffusible pigments were produced.

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6664815824D-fructose-carbon source
6664816988D-ribose-carbon source
6664817268myo-inositol-carbon source
6664817814salicin-carbon source
6664818287L-fucose-carbon source
6664830849L-arabinose-carbon source
6664835962sorbic acid-carbon source
6664816024D-mannose+carbon source
6664816634raffinose+carbon source
6664816899D-mannitol+carbon source
6664817057cellobiose+carbon source
6664817234glucose+carbon source
6664817306maltose+carbon source
6664817754glycerol+carbon source
6664817992sucrose+carbon source
6664823652dextrin+carbon source
6664827082trehalose+carbon source
6664863150D-rhamnose+carbon source
666485291gelatin-degradation
66648milk-degradation
6664817632nitrate-reduction

metabolite production

@refChebi-IDmetaboliteproduction
6664816136hydrogen sulfideno
6664889634melaninno

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitudeenrichment cultureenrichment culture compositionenrichment culture durationenrichment culture temperatureisolation procedure
66498mangrove mudSanya (18° 16' 44.7'' N, 109° 27' 27.1'' E)ChinaCHNAsia18.2791109.458
66648mangrove mud sampleSanyaChinaCHNAsia18.2791109.458ISP4made with 70 % aged seawater in distilled water (instead of pure distilled water), and supplemented with cycloheximide (25 mg ml-1), potassium dichromate (50 mg ml-1) and nystatin (50 mg ml-1)7 days2810 g sediment samples were heated at 55°C for 6 min, then suspended in 100 ml sterile aged seawater and stirred for 30 min. The liquid was plated on inorganic salts/starch [International Streptomyces Project (ISP) 4] using a tenfold dilution series methodand incubated at 28°C for 7 days.

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Mangrove
#Environmental#Aquatic#Sediment
#Host#Plants#Tree

Sequence information

16S sequences

  • @ref: 66498
  • description: Streptomyces sp. strain HA15955 16S ribosomal RNA gene, partial sequence
  • accession: KX090569
  • length: 1489
  • database: ena
  • NCBI tax ID: 2307231

GC content

  • @ref: 66648
  • GC-content: 71.7
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 66498

culture collection no.: DSM 105693, CGMCC 4.7426

straininfo link

  • @ref: 112122
  • straininfo: 399885

literature

  • topic: Phylogeny
  • Pubmed-ID: 30152750
  • title: Streptomyces caeni sp. nov., isolated from mangrove mud.
  • authors: Huang H, Liu M, Zhong W, Mo K, Zhu J, Zou X, Hu Y, Bao S
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.002916
  • year: 2018
  • mesh: Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Rhizophoraceae/*microbiology, Sequence Analysis, DNA, *Soil Microbiology, Streptomyces/*classification/genetics/isolation & purification
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
66498Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-105693Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 105693)
66648Huiqin Huang, Min Liu, Weida Zhong, Kunlian Mo, Jun Zhu, Xiaoxiao Zou, Yonghua Hu, Shixiang BaoStreptomyces caeni sp. nov., isolated from mangrove mud10.1099/ijsem.0.002916IJSEM 68: 3080-3083 201830152750
112122Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID399885.1