Strain identifier

BacDive ID: 159635

Type strain: Yes

Species: Gracilibacillus massiliensis

Strain Designation: Awa-1

Strain history: <- S. Khelaifia, Timone Hospital, Marseille, France; Awa-1 <- Unité de Recherche sur les Maladies Infectieuses et Tropicales Émergentes

NCBI tax ID(s): 1564956 (species)

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General

@ref: 66479

BacDive-ID: 159635

DSM-Number: 29726

keywords: genome sequence, 16S sequence, Bacteria, mesophilic

description: Gracilibacillus massiliensis Awa-1 is a mesophilic bacterium that was isolated from alimentary sea salt.

NCBI tax id

  • NCBI tax id: 1564956
  • Matching level: species

strain history

  • @ref: 66479
  • history: <- S. Khelaifia, Timone Hospital, Marseille, France; Awa-1 <- Unité de Recherche sur les Maladies Infectieuses et Tropicales Émergentes

doi: 10.13145/bacdive159635.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Gracilibacillus
  • species: Gracilibacillus massiliensis
  • full scientific name: Gracilibacillus massiliensis Diop et al. 2017

@ref: 66479

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Caryophanales

family: Bacillaceae

genus: Gracilibacillus

species: Gracilibacillus massiliensis

full scientific name: Gracilibacillus massiliensis Diop et al. 2017

strain designation: Awa-1

type strain: yes

Culture and growth conditions

culture medium

  • @ref: 66479
  • name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/514
  • composition: Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water

culture temp

  • @ref: 66479
  • growth: positive
  • type: growth
  • temperature: 28
  • range: mesophilic

Isolation, sampling and environmental information

isolation

  • @ref: 66479
  • sample type: alimentary sea salt
  • geographic location: Camargue
  • country: France
  • origin.country: FRA
  • continent: Europe

isolation source categories

Cat1Cat2
#Engineered#Food production
#Condition#Saline

Safety information

risk assessment

  • @ref: 66479
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 66479
  • description: Gracilibacillus sp. Awa-1 partial 16S rRNA gene, strain Awa-1
  • accession: LN626645
  • length: 1528
  • database: ena
  • NCBI tax ID: 1564956

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Gracilibacillus massiliensis Awa-1GCA_001458115contigncbi1564956
66792Gracilibacillus massiliensis Awa-12690315889draftimg1564956

GC content

  • @ref: 66479
  • GC-content: 36.05
  • method: sequence analysis

External links

@ref: 66479

culture collection no.: DSM 29726, CSUR P1441

straininfo link

  • @ref: 112109
  • straininfo: 405943

literature

  • topic: Phylogeny
  • Pubmed-ID: 27760679
  • title: Microbial culturomics unravels the halophilic microbiota repertoire of table salt: description of Gracilibacillus massiliensis sp. nov.
  • authors: Diop A, Khelaifia S, Armstrong N, Labas N, Fournier PE, Raoult D, Million M
  • journal: Microb Ecol Health Dis
  • DOI: 10.3402/mehd.v27.32049
  • year: 2016

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
66479Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-29726Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 29726)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
112109Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID405943.1