Strain identifier

BacDive ID: 159625

Type strain: Yes

Species: Streptomyces huasconensis

Strain Designation: HST28

Strain history: <- I. Nouioui, Univ. Newcastle, Newcastle upon Tyne, UK; HST28 <- C. Cortes, Universidad de Chile; HST28

NCBI tax ID(s): 1854574 (species)

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General

@ref: 66460

BacDive-ID: 159625

DSM-Number: 107268

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-positive

description: Streptomyces huasconensis HST28 is an aerobe, mesophilic, Gram-positive bacterium that builds an aerial mycelium and was isolated from soil.

NCBI tax id

  • NCBI tax id: 1854574
  • Matching level: species

strain history

  • @ref: 66460
  • history: <- I. Nouioui, Univ. Newcastle, Newcastle upon Tyne, UK; HST28 <- C. Cortes, Universidad de Chile; HST28

doi: 10.13145/bacdive159625.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces huasconensis
  • full scientific name: Streptomyces huasconensis Cortés-Albayay et al. 2019

@ref: 66460

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Streptomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces huasconensis

full scientific name: Streptomyces huasconensis Cortés-Albaya et al. 2019

strain designation: HST28

type strain: yes

Morphology

cell morphology

  • @ref: 67976
  • gram stain: positive

colony morphology

@refcolony colormedium usedincubation period
69240Fawn brown (8007)suter with tyrosine
69240Fawn brown (8007)suter without tyrosine
69240Black brown (8022)ISP 7
69240Black brown (8022)ISP 4
69240Black brown (8022)ISP 5
69240Beige (1001)ISP 6
67976pinkish-whiteGYM agar7 days
69240Ochre brown (8001)ISP 3
69240Sepia brown (8014)ISP 2

multicellular morphology

@refforms multicellular complexcomplex namecomplex colormedium name
69240yesAerial myceliumCream (9001), reed green (6013), yellow grey (7034)ISP 2
69240yesAerial myceliumCream (9001), light pink (3015)ISP 3
69240yesAerial myceliumPure white (9010)ISP 4
69240yesAerial myceliumPure white (9010), pebble grey (7032)ISP 5
69240noAerial myceliumISP 6
69240yesAerial myceliumCream (9001), yellow grey (7034)ISP 7
69240yesAerial myceliumCream (9002)suter with tyrosine
69240yesAerial myceliumCream (9001)suter without tyrosine

pigmentation

@refproductionnamecolor
69240noMelanin
69240yessoluble pigmentFawn brown (8007)

Culture and growth conditions

culture medium

  • @ref: 66460
  • name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/65
  • composition: Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
66460positivegrowth28mesophilic
67976positiveoptimum28mesophilic

culture pH

  • @ref: 67976
  • ability: positive
  • type: optimum
  • pH: 8.0-9.0
  • PH range: alkaliphile

Physiology and metabolism

oxygen tolerance

  • @ref: 67976
  • oxygen tolerance: aerobe

halophily

@refsaltgrowthtested relationconcentration
67976NaClpositiveoptimum10 %(w/v)
69240NaClpositivegrowth0-5 %

observation

@refobservation
67976aerial mycelium
67976ll-diaminopimelic acid in its peptidoglycan layer
67976galactose, glucose, ribose and traces of arabinose and mannose as whole-cell sugars
67976the polar lipid profile contains phosphatidylmethylethanolamine, phosphatidylinositol, aminolipid, glycophospholipid and an unidentified lipid
67976MK-9(H6), MK-9(H8) and MK-9(H4) are the predominating menaquinones

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6924022599arabinose+growth
6797615824D-fructose+carbon source
6797678697D-fructose 6-phosphate+carbon source
6797617268myo-inositol+carbon source
6797616467L-arginine+nitrogen source
67976L-alanine 4-nitroanilide+nitrogen source
6797613705acetoacetate+builds acid from
67976161680aztreonam+growth
6797648607lithium chloride+growth
67976100147nalidixic acid+growth
6797675198tetrazolium blue+growth
6924062968cellulose-growth
6924028757fructose+growth
6924017234glucose+growth
6924017268inositol+growth
6924037684mannose+growth
6924016634raffinose-growth
6924026546rhamnose-growth
6924017992sucrose-growth
6924018222xylose+growth
6837917632nitrate-reduction
683794853esculin-hydrolysis
6837916199urea-hydrolysis
683795291gelatin+hydrolysis
6837917634D-glucose-fermentation
6837916988D-ribose-fermentation
6837965327D-xylose-fermentation
6837916899D-mannitol-fermentation
6837917306maltose-fermentation
6837917716lactose-fermentation
6837917992sucrose-fermentation
6837928087glycogen-fermentation

enzymes

@refvalueactivityec
68379gelatinase+
68379urease-3.5.1.5
68379beta-glucosidase-3.2.1.21
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379alpha-glucosidase-3.2.1.20
68379beta-galactosidase-3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase+3.1.3.1
68379pyrrolidonyl arylamidase-3.4.19.3
68379pyrazinamidase-3.5.1.B15
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382trypsin+3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-fucosidase-3.2.1.51

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    67976C11:0 iso0.4
    67976C13:0 iso0.2
    67976C13:0 anteiso0.2
    67976C14:0 iso1.2
    67976C14:00.3
    67976C15:0 iso8.2
    67976C15:0 anteiso31.8
    67976C15:01
    67976C16:1 iso H1.6
    67976C16:0 iso10.1
    67976C16:1 w9c3.3
    67976C16:05
    67976C16:0 9-methyl4.3
    67976C17:1 anteiso C8.1
    67976C17:0 iso4.1
    67976C17:0 anteiso18.3
    67976C17:1 w9c0.4
    67976C17:0 cyclo0.7
    67976C17:00.3
  • type of FA analysis: whole cell analysis
  • incubation medium: GYM
  • agar/liquid: agar
  • software version: Sherlock 4.5
  • library/peak naming table: ACTIN6
  • system: MIS MIDI
  • method/protocol: Miller 1982

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYG
69240---+------+--------

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
69240+++/--++/-+/-+-++-----++/--

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitude
66460soilTarapaca Region, Salar de Huasco (20° 19' 43'' S, 68° 50' 19'' W)ChileCHLMiddle and South America-20.3286-68.8386
67976arid soil samples, in complete absence of vegetation, in an athalassohaline and polyextreme high-altitude saline wetland (3800 m.a.s.l)Salar de Huasco, Altiplano, the high plateau of the AndesPeruPERMiddle and South America-20.3286-68.8386

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

Sequence information

16S sequences

  • @ref: 66460
  • description: Streptomyces huasconensis strain HST28 16S ribosomal RNA gene, complete sequence
  • accession: KX130877
  • length: 1517
  • database: ena
  • NCBI tax ID: 1854574

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Streptomyces huasconensis HST282866435396draftimg1854574
67976Streptomyces huasconensis HST28GCA_004010275contigncbi1854574

GC content

@refGC-contentmethod
6646071.5sequence analysis
6797671.5genome sequence analysis

External links

@ref: 66460

culture collection no.: DSM 107268, CECT 9648

straininfo link

  • @ref: 112099
  • straininfo: 401780

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny31135331Streptomyces huasconensis sp. nov., an haloalkalitolerant actinobacterium isolated from a high altitude saline wetland at the Chilean Altiplano.Cortes-Albayay C, Dorador C, Schumann P, Andrews B, Asenjo J, Nouioui IInt J Syst Evol Microbiol10.1099/ijsem.0.0034682019*Altitude, Bacterial Typing Techniques, Base Composition, Chile, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, *Salinity, Sequence Analysis, DNA, Streptomyces/*classification/isolation & purification, Vitamin K 2/analogs & derivatives/chemistry, *WetlandsTranscriptome
Phylogeny31931565Downsizing Class II Lasso Peptides: Genome Mining-Guided Isolation of Huascopeptin Containing the First Gly1-Asp7 Macrocycle.Cortes-Albayay C, Jarmusch SA, Trusch F, Ebel R, Andrews BA, Jaspars M, Asenjo JAJ Org Chem10.1021/acs.joc.9b022312020Multigene Family, *Peptides, *Streptomyces/genetics
Transcriptome32908238Cutting the Gordian knot: early and complete amino acid sequence confirmation of class II lasso peptides by HCD fragmentation.Jarmusch SA, Feldmann I, Blank-Landeshammer B, Cortes-Albayay C, Castro JF, Andrews B, Asenjo JA, Sickmann A, Ebel R, Jaspars MJ Antibiot (Tokyo)10.1038/s41429-020-00369-z2020Amino Acid Sequence, Mass Spectrometry/*methods, Peptides, Cyclic/chemistry/*genetics, Sequence Analysis, Protein/*methods
34408734Antimicrobial Activity and Identification of the Biosynthetic Gene Cluster of X-14952B From Streptomyces sp. 135.Li N, Chen S, Yan Z, Han J, Ta Y, Pu T, Wang YFront Microbiol10.3389/fmicb.2021.7030932021

Reference

@idauthorstitledoi/urlcataloguejournal
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
66460Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-107268Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 107268)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
67976Carlos Cortés-Albayay, Cristina Dorador, Peter Schumann, Barbara Andrews, Juan Asenjo, Imen NouiouiStreptomyces huasconensis sp. nov., an haloalkalitolerant actinobacterium isolated from a high altitude saline wetland at the Chilean Altiplano10.1099/ijsem.0.003468IJSEM 69: 2315-2322 2019
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
69240Wink, J.Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweighttps://cdn.dsmz.de/wink/DSM%20107268.pdf
112099Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID401780.1