Strain identifier
BacDive ID: 159311
Type strain: 
Species: Ktedonosporobacter rubrisoli
Strain Designation: SCAWS-G2
Strain history: <- Y. Huang; Institute of Microbiology, Chinese Academy of Sciences, Beijing, CHINA; SCAWS-G2
NCBI tax ID(s): 2509675 (species)
version 9.1 (current version)
General
@ref: 66134
BacDive-ID: 159311
DSM-Number: 105258
keywords: genome sequence, 16S sequence, Bacteria, aerobe, spore-forming, Gram-positive, filament-shaped, pigmented
description: Ktedonosporobacter rubrisoli SCAWS-G2 is an aerobe, spore-forming, Gram-positive bacterium that has a Pale brown pigmentation and was isolated from red soil.
NCBI tax id
- NCBI tax id: 2509675
- Matching level: species
strain history
- @ref: 66134
- history: <- Y. Huang; Institute of Microbiology, Chinese Academy of Sciences, Beijing, CHINA; SCAWS-G2
doi: 10.13145/bacdive159311.20240916.9.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/chloroflexota
- domain: Bacteria
- phylum: Chloroflexota
- class: Ktedonobacteria
- order: Ktedonobacterales
- family: Ktedonosporobacteraceae
- genus: Ktedonosporobacter
- species: Ktedonosporobacter rubrisoli
- full scientific name: Ktedonosporobacter rubrisoli Yan et al. 2020
@ref: 66134
domain: Bacteria
phylum: Chloroflexi
class: Ktedonobacteria
order: Ktedonobacterales
family: Ktedonosporobacteraceae
genus: Ktedonosporobacter
species: Ktedonosporobacter rubrisoli
full scientific name: Ktedonosporobacter rubrisoli Yan et al. 2019
strain designation: SCAWS-G2
type strain: yes
Morphology
cell morphology
- @ref: 69565
- gram stain: positive
- cell shape: filament-shaped
- motility: no
colony morphology
- @ref: 69565
- colony size: 1-3 mm
- colony color: pale brown
- incubation period: 2 days
- medium used: GYM Agar
pigmentation
- @ref: 69565
- production: yes
- color: Pale brown
multimedia
- @ref: 66134
- multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_105258.jpg
- caption: Medium 65 pH5 28°C
- intellectual property rights: © Leibniz-Institut DSMZ
Culture and growth conditions
culture medium
| @ref | name | growth | link | composition |
|---|---|---|---|---|
| 66134 | GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) | yes | https://mediadive.dsmz.de/medium/65 | Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water |
| 69565 | GYM Agar | yes |
culture temp
| @ref | growth | type | temperature |
|---|---|---|---|
| 66134 | positive | growth | 28 |
| 69565 | positive | growth | 25-45 |
| 69565 | positive | optimum | 28-35 |
culture pH
| @ref | ability | type | pH |
|---|---|---|---|
| 69565 | positive | growth | 4.0-7.0 |
| 69565 | positive | optimum | 5.0-6.0 |
Physiology and metabolism
oxygen tolerance
- @ref: 69565
- oxygen tolerance: aerobe
spore formation
- @ref: 69565
- spore description: spore is 1.3-1.6 µm in diameter, but not motile
- type of spore: spore
- spore formation: yes
halophily
| @ref | salt | growth | tested relation | concentration |
|---|---|---|---|---|
| 69565 | NaCl | no | growth | 2 %(w/v) |
| 69565 | NaCl | positive | growth | 1 %(w/v) |
observation
- @ref: 69565
- observation: Good growth occurs on ISP 2, GYM, DSMZ 554 and Bennett's agars. No growth occurs on ISP 4, ISP 5 or ISP 7 media. Colonies are forms branched vegetative mycelium, and produces sparse, white aerial hyphae that bear spherical spores in nutrition-poor media such as HSA5 agar. Mycelium has a diameter of approximately 0.9-1.2 µm and spores are 1.3-1.6 µm in diameter. Spores are born on short sporophores from aerial hyphae, and the dense spores form a grape-like cluster on mycelium.
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 69565 | 16136 | hydrogen sulfide | - | assimilation |
| 69565 | 18305 | arbutin | - | assimilation |
| 69565 | 15824 | D-fructose | - | assimilation |
| 69565 | 65327 | D-xylose | - | assimilation |
| 69565 | 4853 | esculin | - | assimilation |
| 69565 | 30849 | L-arabinose | - | assimilation |
| 69565 | 62345 | L-rhamnose | - | assimilation |
| 69565 | 29864 | mannitol | - | assimilation |
| 69565 | 17268 | myo-inositol | - | assimilation |
| 69565 | 16634 | raffinose | - | assimilation |
| 69565 | 50144 | sodium pyruvate | - | assimilation |
| 69565 | 30911 | sorbitol | - | assimilation |
| 69565 | 30031 | succinate | - | assimilation |
| 69565 | 17992 | sucrose | - | assimilation |
| 69565 | casein | - | hydrolysis | |
| 69565 | 62968 | cellulose | - | hydrolysis |
| 69565 | 17029 | chitin | - | hydrolysis |
| 69565 | 5291 | gelatin | - | hydrolysis |
| 69565 | 28017 | starch | - | hydrolysis |
| 69565 | 16261 | chitosan | + | assimilation |
| 69565 | 12936 | D-galactose | + | assimilation |
| 69565 | 17634 | D-glucose | + | assimilation |
| 69565 | 23652 | dextrin | + | assimilation |
| 69565 | 91260 | disodium malate | + | assimilation |
| 69565 | 6731 | melezitose | + | assimilation |
| 69565 | 320061 | methyl alpha-D-glucopyranoside | + | assimilation |
| 69565 | 30623 | oxalate | + | assimilation |
| 69565 | 17814 | salicin | + | assimilation |
| 69565 | 62983 | sodium malonate | + | assimilation |
| 69565 | 17632 | nitrate | + |
metabolite production
- @ref: 69565
- Chebi-ID: 16136
- metabolite: hydrogen sulfide
- production: no
enzymes
- @ref: 69565
- value: catalase
- activity: +
- ec: 1.11.1.6
fatty acid profile
fatty acids
@ref fatty acid percentage 69565 C16:0 24.4 69565 C16:0 10-methyl/ C17:1 iso w9c 14.4 69565 C16:1 2OH 26.5 69565 C17:0 iso 23.1 - type of FA analysis: whole cell analysis
- incubation medium: GYM
- incubation temperature: 28
- incubation time: 2-3
- software version: Sherlock 6.0
- library/peak naming table: TSBA6
- system: MIS MIDI
Isolation, sampling and environmental information
isolation
| @ref | sample type | geographic location | country | origin.country | continent | latitude | longitude |
|---|---|---|---|---|---|---|---|
| 66134 | red soil | Wushan (N 29° 44' -29°46', E 115° 37'-115° 41'), northern Jiangxi Province | China | CHN | Asia | 29.7333 | 115.617 |
| 69565 | the surface layer red soils sampled at Wushan, northern Jiangxi Province, PR China. | Wushan, northern Jiangxi Province | China | CHN | Asia |
isolation source categories
- Cat1: #Environmental
- Cat2: #Terrestrial
- Cat3: #Soil
Sequence information
16S sequences
| @ref | description | accession | length | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66134 | Ktedonosporobacter rubrisoli strain SCAWS-G2 16S ribosomal RNA gene, partial sequence | KY427895 | 1480 | nuccore | 2509675 |
| 66134 | Ktedonosporobacter rubrisoli strain SCAWS-G2 16S ribosomal RNA gene, partial sequence | KY427896 | 1480 | nuccore | 2509675 |
| 66134 | Ktedonosporobacter rubrisoli strain SCAWS-G2 16S ribosomal RNA gene, partial sequence | MK318920 | 1474 | nuccore | 2509675 |
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Ktedonosporobacter rubrisoli SCAWS-G2 | GCA_004208415 | complete | ncbi | 2509675 |
| 69565 | genome sequence | CP035758 | ncbi | 2509675 |
GC content
- @ref: 69565
- GC-content: 51.8
- method: genome sequence analysis
Genome-based predictions
predictions
| @ref | trait | model | description | prediction | confidence | training_data |
|---|---|---|---|---|---|---|
| 69481 | spore-forming | spore-forming | Ability to form endo- or exospores | no | 50 | no |
| 69480 | gram-positive | gram-positive | Positive reaction to Gram-staining | yes | 77.561 | no |
| 69480 | anaerobic | anaerobic | Ability to grow under anoxygenic conditions (including facultative anaerobes) | no | 93.91 | yes |
| 69480 | aerobic | aerobic | Ability to grow under oxygenic conditions (including facultative aerobes) | yes | 76.599 | yes |
| 69480 | spore-forming | spore-forming | Ability to form endo- or exospores | yes | 63.406 | no |
| 69480 | thermophilic | thermophile | Ability to grow at temperatures above or equal to 45°C | no | 85.093 | yes |
| 69480 | flagellated | motile2+ | Ability to perform flagellated movement | no | 82.938 | no |
External links
@ref: 66134
culture collection no.: DSM 105258, CGMCC 1.16132
straininfo link
- @ref: 111810
- straininfo: 401617
literature
- topic: Phylogeny
- Pubmed-ID: 31730027
- title: Ktedonosporobacter rubrisoli gen. nov., sp. nov., a novel representative of the class Ktedonobacteria, isolated from red soil, and proposal of Ktedonosporobacteraceae fam. nov.
- authors: Yan B, Guo X, Liu M, Huang Y
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijsem.0.003864
- year: 2020
- mesh: Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, China, Chloroflexi/*classification/isolation & purification, DNA, Bacterial/genetics, Fatty Acids/chemistry, Glycolipids/chemistry, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology
- topic2: Transcriptome
Reference
| @id | authors | title | doi/url | catalogue |
|---|---|---|---|---|
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |
| 66134 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-105258 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 105258) | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |
| 69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | Predictions based on the model GenomeNet Sporulation v. 1 | 10.21203/rs.3.rs-2527258/v1 | |
| 69565 | Bingfa Yan, Xiaoxuan Guo, Minghao Liu and Ying Huang | Ktedonosporobacter rubrisoli gen. nov., sp. nov., a novel representative of the class Ktedonobacteria, isolated from red soil, and proposal of Ktedonosporobacteraceae fam. nov. | 10.1099/ijsem.0.003864 | |
| 111810 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID401617.1 |