Strain identifier
BacDive ID: 158790
Type strain:
Species: Amycolatopsis silviterrae
Strain Designation: C12CA1
NCBI tax ID(s): 1656914 (species)
version 8.1 (current version)
General
@ref: 65547
BacDive-ID: 158790
keywords: 16S sequence, Bacteria, aerobe, spore-forming, Gram-positive, rod-shaped, pigmented
description: Amycolatopsis silviterrae C12CA1 is an aerobe, spore-forming, Gram-positive bacterium that builds a substrate mycelium and has a greenish-yellow pigmentation.
NCBI tax id
- NCBI tax id: 1656914
- Matching level: species
doi: 10.13145/bacdive158790.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Pseudonocardiales
- family: Pseudonocardiaceae
- genus: Amycolatopsis
- species: Amycolatopsis silviterrae
- full scientific name: Amycolatopsis silviterrae Jamjan et al. 2018
@ref: 65547
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Pseudonocardiales
family: Pseudonocardiaceae
genus: Amycolatopsis
species: Amycolatopsis silviterrae
strain designation: C12CA1
type strain: yes
Morphology
cell morphology
- @ref: 65547
- gram stain: positive
- cell shape: rod-shaped
multicellular morphology
@ref | forms multicellular complex | complex name | complex color |
---|---|---|---|
65547 | yes | substrate mycelium | yellowish-brown |
65547 | yes | aerial mycelium |
pigmentation
- @ref: 65547
- production: yes
- color: greenish-yellow
Culture and growth conditions
culture medium
@ref | name | growth |
---|---|---|
65547 | ISP 2 | yes |
65547 | ISP 3 | yes |
65547 | ISP 4 | yes |
65547 | ISP 6 | yes |
65547 | ISP 7 | yes |
65547 | Nutrient agar (NA) | yes |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
65547 | positive | growth | 15-40 | |
65547 | positive | optimum | 25-30 | mesophilic |
culture pH
@ref | ability | type | pH | PH range |
---|---|---|---|---|
65547 | positive | growth | 5-12 | alkaliphile |
65547 | positive | optimum | 7-10 |
Physiology and metabolism
oxygen tolerance
- @ref: 65547
- oxygen tolerance: aerobe
spore formation
- @ref: 65547
- spore description: greenish-yellow aerial spore
- spore formation: yes
halophily
- @ref: 65547
- salt: NaCl
- growth: positive
- tested relation: growth
- concentration: 0-7 %(w/v)
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
65547 | 16708 | adenine | - | hydrolysis |
65547 | casein | + | hydrolysis | |
65547 | 17057 | cellobiose | + | carbon source |
65547 | 62968 | cellulose | - | hydrolysis |
65547 | 17029 | chitin | - | hydrolysis |
65547 | 15824 | D-fructose | + | carbon source |
65547 | 12936 | D-galactose | + | carbon source |
65547 | 16899 | D-mannitol | + | carbon source |
65547 | 16988 | D-ribose | + | carbon source |
65547 | 65327 | D-xylose | + | carbon source |
65547 | 17754 | glycerol | - | carbon source |
65547 | 17368 | hypoxanthine | + | hydrolysis |
65547 | 30849 | L-arabinose | + | carbon source |
65547 | 62345 | L-rhamnose | - | carbon source |
65547 | 17716 | lactose | + | carbon source |
65547 | 37684 | mannose | + | carbon source |
65547 | 28053 | melibiose | - | carbon source |
65547 | 17268 | myo-inositol | + | carbon source |
65547 | 17632 | nitrate | - | reduction |
65547 | 16634 | raffinose | + | carbon source |
65547 | 17814 | salicin | + | carbon source |
65547 | 30911 | sorbitol | - | carbon source |
65547 | 27922 | sorbose | - | carbon source |
65547 | 28017 | starch | - | hydrolysis |
65547 | 17992 | sucrose | - | carbon source |
65547 | 27082 | trehalose | - | carbon source |
65547 | 53426 | tween 80 | + | hydrolysis |
65547 | 15318 | xanthine | + | hydrolysis |
65547 | 17151 | xylitol | - | carbon source |
enzymes
@ref | value | activity | ec |
---|---|---|---|
65547 | alkaline phosphatase | + | 3.1.3.1 |
65547 | esterase (C 4) | + | |
65547 | esterase Lipase (C 8) | + | |
65547 | leucine arylamidase | + | 3.4.11.1 |
65547 | valine arylamidase | + | |
65547 | cystine arylamidase | + | 3.4.11.3 |
65547 | trypsin | + | 3.4.21.4 |
65547 | alpha-chymotrypsin | + | 3.4.21.1 |
65547 | acid phosphatase | + | 3.1.3.2 |
65547 | naphthol-AS-BI-phosphohydrolase | + | |
65547 | beta-galactosidase | + | 3.2.1.23 |
65547 | N-acetyl-beta-glucosaminidase | + | 3.2.1.52 |
65547 | alpha-mannosidase | + | 3.2.1.24 |
65547 | lipase (C 14) | - | |
65547 | alpha-galactosidase | - | 3.2.1.22 |
65547 | beta-glucuronidase | - | 3.2.1.31 |
65547 | alpha-glucosidase | - | 3.2.1.20 |
65547 | beta-glucosidase | - | 3.2.1.21 |
65547 | alpha-fucosidase | - | 3.2.1.51 |
fatty acid profile
fatty acids
@ref fatty acid percentage 65547 C14:0 1.5 65547 C16:0 27.9 65547 C17:0 2.5 65547 C18:0 1.7 65547 C15:1ω6c 0.8 65547 C14:0 iso 2.3 65547 C15:0 iso 24.2 65547 C16:0 iso 17.5 65547 C17:0 iso 6.3 65547 C15:0 anteiso 1.6 65547 C16:0 anteiso 0.9 65547 C17:0 anteiso 2.3 65547 C16:1ω7c / C16:1ω6c 7.7 65547 C16:0 10-methyl / iso-C17:1ω9c 0.1 - type of FA analysis: whole cell analysis
- incubation medium: ISP2
- incubation temperature: 28
- incubation time: 5
- library/peak naming table: RTSBA6
- system: MIS MIDI
- method/protocol: Sasser, 2001
Isolation, sampling and environmental information
isolation
- @ref: 65547
- sample type: forest soil
- geographic location: Chulabhorn dam, Chaiyaphum Province
- country: Thailand
- origin.country: THA
- continent: Asia
- enrichment culture: actinomycetes isolation agar
- enrichment culture composition: 4 g sodium propionate, 2 g sodium caseinate, 0.5 g K2HPO4, 0.1 g asparagine, 0.1 g MgSO4 . 7H2O, 1 g FeSO4 . 7H2O, 5 ml glycerol, 15 g agar and 1 l distilled water supplemented with cycloheimide (50 mg/l) and nalidixic acid (50 mg/l).
- enrichment culture duration: 3-4 weeks
- enrichment culture temperature: 28
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Environmental | #Terrestrial | #Forest |
#Environmental | #Terrestrial | #Soil |
Sequence information
16S sequences
- @ref: 65547
- description: 16S rRNA gene sequence
- accession: KR818707
- database: nuccore
GC content
- @ref: 65547
- GC-content: 69.8
- method: high performance liquid chromatography (HPLC)
External links
@ref: 65547
culture collection no.: TBRC 1456, NBRC 111116
literature
- topic: Phylogeny
- Pubmed-ID: 29533177
- title: Amycolatopsis silviterrae sp. nov., isolated from forest soil.
- authors: Jamjan W, Suriyachadkun C, Tanasupawat S, Sakai K, Tashiro Y, Okugawa Y, Tongpim S
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijsem.0.002687
- year: 2018
- mesh: Actinomycetales/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, *Forests, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Thailand, Vitamin K 2/analogs & derivatives/chemistry
- topic2: Transcriptome
Reference
@id | authors | title | doi/url | journal | pubmed |
---|---|---|---|---|---|
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | ||
65547 | Winai Jamjan, Chanwit Suriyachadkun, Somboon Tanasupawat, Kenji Sakai, Yukihiro Tashiro, Yuki Okugawa and Saowanit Tongpim | Amycolatopsis silviterrae sp. nov., isolated from forest soil | 10.1099/ijsem.0.002687 | IJSEM 68: 1455-1460 2018 | 29533177 |