Strain identifier

BacDive ID: 158648

Type strain: Yes

Species: Mesobacillus fermenti

Strain Designation: Bf-1

Strain history: T. Tamura and I. Yumoto; AIST, Japan; Bf-1.

NCBI tax ID(s): 1920257 (species)

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General

@ref: 65373

BacDive-ID: 158648

keywords: 16S sequence, Bacteria, facultative anaerobe, Gram-positive, motile, rod-shaped, colony-forming

description: Mesobacillus fermenti Bf-1 is a facultative anaerobe, Gram-positive, motile bacterium that forms circular colonies and was isolated from indigo fermentation liquor used for dyeing. This liquor was produced from sukumo .

NCBI tax id

  • NCBI tax id: 1920257
  • Matching level: species

strain history

  • @ref: 67770
  • history: T. Tamura and I. Yumoto; AIST, Japan; Bf-1.

doi: 10.13145/bacdive158648.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Mesobacillus
  • species: Mesobacillus fermenti
  • full scientific name: Mesobacillus fermenti (Hirota et al. 2018) Gupta et al. 2020

  • synonyms

    • @ref: 20215
    • synonym: Bacillus fermenti

@ref: 65373

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Caryophanales

family: Bacillaceae

genus: Bacillus

species: Bacillus fermenti

strain designation: Bf-1

type strain: yes

Morphology

cell morphology

  • @ref: 65373
  • gram stain: positive
  • cell length: 2.2-3.8 µm
  • cell width: 0.8-1.2 µm
  • cell shape: rod-shaped
  • motility: yes
  • flagellum arrangement: subpolar

colony morphology

  • @ref: 65373
  • colony size: 0.5-2 mm
  • colony color: creamy-white
  • colony shape: circular
  • medium used: peptone-yeast extract agar

Culture and growth conditions

culture medium

  • @ref: 65373
  • name: peptone-yeast extract agar
  • growth: yes

culture temp

@refgrowthtypetemperaturerange
65373positivegrowth15-45
65373positiveoptimum33-40
67770positivegrowth28mesophilic

culture pH

@refabilitytypepHPH range
65373positivegrowth8-12alkaliphile
65373positiveoptimum10

Physiology and metabolism

oxygen tolerance

  • @ref: 65373
  • oxygen tolerance: facultative anaerobe

halophily

@refsaltgrowthtested relationconcentration
65373NaClpositivegrowth0-7 %
65373NaClpositiveoptimum0 %

observation

  • @ref: 67770
  • observation: quinones: MK-7

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6537327613amygdalin-builds acid from
6537318305arbutin+builds acid from
6537317057cellobiose-builds acid from
6537315963ribitol-builds acid from
6537317108D-arabinose-builds acid from
6537318333D-arabitol-builds acid from
6537315824D-fructose-builds acid from
6537328847D-fucose-builds acid from
6537312936D-galactose-builds acid from
6537317634D-glucose-builds acid from
6537362318D-lyxose-builds acid from
6537316899D-mannitol-builds acid from
6537316024D-mannose-builds acid from
6537316988D-ribose+builds acid from
6537317924D-sorbitol-builds acid from
6537316443D-tagatose-builds acid from
6537365327D-xylose-builds acid from
6537316813galactitol-builds acid from
6537317113erythritol-builds acid from
65373esculin ferric citrate+builds acid from
6537328066gentiobiose-builds acid from
6537317754glycerol-builds acid from
6537328087glycogen-builds acid from
6537331695indigo carmine+reduction
6537317268myo-inositol-builds acid from
6537315443inulin-builds acid from
6537330849L-arabinose-builds acid from
6537318403L-arabitol-builds acid from
6537318287L-fucose-builds acid from
6537362345L-rhamnose-builds acid from
6537317266L-sorbose+builds acid from
6537365328L-xylose-builds acid from
6537317716lactose-builds acid from
6537317306maltose-builds acid from
653736731melezitose-builds acid from
6537328053melibiose-builds acid from
65373320061methyl alpha-D-glucopyranoside-builds acid from
6537343943methyl alpha-D-mannoside-builds acid from
6537374863methyl beta-D-xylopyranoside-builds acid from
65373506227N-acetylglucosamine-builds acid from
6537317632nitrate+reduction
65373potassium 2-dehydro-D-gluconate+builds acid from
65373potassium 5-dehydro-D-gluconate+builds acid from
6537332032potassium gluconate-builds acid from
6537316634raffinose-builds acid from
6537317814salicin-builds acid from
6537328017starch-builds acid from
6537317992sucrose-builds acid from
6537327082trehalose-builds acid from
6537332528turanose+builds acid from
6537317151xylitol-builds acid from

metabolite production

@refChebi-IDmetaboliteproduction
6537315688acetoinno
6537316136hydrogen sulfideno

metabolite tests

  • @ref: 65373
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: -

enzymes

@refvalueactivityec
65373catalase+1.11.1.6
65373cytochrome oxidase+1.9.3.1
65373alkaline phosphatase+3.1.3.1
65373esterase (C 4)+
65373esterase Lipase (C 8)+
65373leucine arylamidase+3.4.11.1
65373valine arylamidase+
65373chymotrypsin+3.4.4.5
65373acid phosphatase+3.1.3.2
65373naphthol-AS-BI-phosphohydrolase+
65373alpha-glucosidase+3.2.1.20
65373arginine dihydrolase-3.5.3.6
65373lysine decarboxylase-4.1.1.18
65373ornithine decarboxylase-4.1.1.17
65373lipase (C 14)-
65373cystine arylamidase-3.4.11.3
65373trypsin-3.4.21.4
65373alpha-galactosidase-3.2.1.22
65373beta-galactosidase-3.2.1.23
65373beta-glucuronidase-3.2.1.31
65373beta-glucosidase-3.2.1.21
65373N-acetyl-beta-glucosaminidase-3.2.1.52
65373alpha-mannosidase-3.2.1.24
65373alpha-fucosidase-3.2.1.51
65373urease+3.5.1.5

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitudeenrichment cultureenrichment culture compositionenrichment culture durationenrichment culture temperatureisolation procedure
65373indigo fermentation liquor used for dyeing. This liquor was produced from sukumo (composted Polygonum indigo leaves)Date City, HokkaidoJapanJPNAsia42.4708140.875indigo carmine-peptone/yeast extract/alkaline(pH 10) consisting of 8.0 g peptone (Kyokuto), 3.0 g yeast extract (Merck), 2.0 g indigo carmine and 15.0 g agar in 1 l NaHCO3/Na2CO3 buffer (100 mM; pH 10) prepared using deionized water, and In-RCA medium (pH 10). The RCA contained 2.0 g indigo carmine in 1 l NaHCO3/Na2CO3 buffer (100 mM; pH 10) prepared using deionized water. A tenfold concentrated stock of each alkaline solution, which was used in each medium, was sterilized separately and then mixed with the primary autoclaved body of each2 days27An aliquot of the sample (0.5 ml) was diluted 10-4 10-5 times in series using 4.5 ml of physiological saline and inoculated into In-PYA medium. The inoculated media were incubated at 27°C for 2 days under an anaerobic N 2 gas-exchange jar.
67770Indigo fermentation liquor used for dyeingDate City, HokkaidoJapanJPNAsia

isolation source categories

Cat1Cat2Cat3
#Host#Plants#Herbaceous plants (Grass,Crops)
#Engineered#Industrial#Industrial production
#Host Body-Site#Plant#Leaf (Phyllosphere)
#Environmental#Aquatic

taxonmaps

  • @ref: 69479
  • File name: preview.99_182852.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_3;96_292;97_834;98_119115;99_182852&stattab=map
  • Last taxonomy: Bacillus fermenti subclade
  • 16S sequence: LC197810
  • Sequence Identity:
  • Total samples: 18
  • soil counts: 2
  • aquatic counts: 13
  • animal counts: 3

Sequence information

16S sequences

  • @ref: 65373
  • description: 16S rRNA gene sequence
  • accession: LC197810
  • database: nuccore

GC content

  • @ref: 65373
  • GC-content: 41.7
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 65373

culture collection no.: JCM 31807, NCIMB 15079

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
65373Kikue Hirota, Masatoshi Nishita, Zhihao Tu, Hidetoshi Matsuyama, Isao YumotoBacillus fermenti sp. nov., an indigo-reducing obligate alkaliphile isolated from indigo fermentation liquor for dyeing10.1099/ijsem.0.002636IJSEM 68: 1123-1129 201829458563
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/