Strain identifier

BacDive ID: 158648

Type strain: Yes

Species: Mesobacillus fermenti

Strain Designation: Bf-1

Culture col. no.: JCM 31807, NCIMB 15079

Strain history: T. Tamura and I. Yumoto; AIST, Japan; Bf-1.

NCBI tax ID(s): 1920257 (species)

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Mesobacillus fermenti Bf-1 is a facultative anaerobe, Gram-positive, motile bacterium that forms circular colonies and was isolated from indigo fermentation liquor used for dyeing. This liquor was produced from sukumo .

  1. colony-forming
  2. Gram-positive
  3. motile
  4. rod-shaped
  5. facultative anaerobe
  6. 16S sequence
  7. Bacteria
  • Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental informationarrow_down
  • [Ref.: #65373] Sample type/isolated from indigo fermentation liquor used for dyeing. This liquor was produced from sukumo (composted Polygonum indigo leaves)
    [Ref.: #65373] Geographic location (country and/or sea, region) Date City, Hokkaido
    [Ref.: #65373] Country Japan
    [Ref.: #65373] Country ISO 3 Code JPN
    [Ref.: #65373] Continent Asia
    [Ref.: #65373] Geographic location 42.4708°/140.8750°
    [Ref.: #65373] Enrichment culture indigo carmine-peptone/yeast extract/alkaline
    [Ref.: #65373] Enrichment culture composition (pH 10) consisting of 8.0 g peptone (Kyokuto), 3.0 g yeast extract (Merck), 2.0 g indigo carmine and 15.0 g agar in 1 l NaHCO3/Na2CO3 buffer (100 mM; pH 10) prepared using deionized water, and In-RCA medium (pH 10). The RCA contained 2.0 g indigo carmine in 1 l NaHCO3/Na2CO3 buffer (100 mM; pH 10) prepared using deionized water. A tenfold concentrated stock of each alkaline solution, which was used in each medium, was sterilized separately and then mixed with the primary autoclaved body of each
    [Ref.: #65373] Enrichment culture duration 2 days
    [Ref.: #65373] Enrichment culture temperature 27  ̊C
    [Ref.: #65373] Isolation/enrichment procedure An aliquot of the sample (0.5 ml) was diluted 10-4 10-5 times in series using 4.5 ml of physiological saline and inoculated into In-PYA medium. The inoculated media were incubated at 27°C for 2 days under an anaerobic N 2 gas-exchange jar.
     
    [Ref.: #67770] Sample type/isolated from Indigo fermentation liquor used for dyeing
    [Ref.: #67770] Geographic location (country and/or sea, region) Date City, Hokkaido
    [Ref.: #67770] Country Japan
    [Ref.: #67770] Country ISO 3 Code JPN
    [Ref.: #67770] Continent Asia
    * marker position based on {}
    Isolation sources categories
    #Host #Plants #Herbaceous plants (Grass,Crops)
    #Engineered #Industrial #Industrial production
    #Host Body-Site #Plant #Leaf (Phyllosphere)
    #Environmental #Aquatic    -   

    Global distribution of 16S sequence LC197810 (>99% sequence identity) for Bacillus fermenti subclade from Microbeatlas

    Aquatic Samples 13
    Soil Samples 2
    Animal Samples 3
    Plant Samples
    Total Samples 18

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