[Ref.: #65373] |
Sample type/isolated from |
indigo fermentation liquor used for dyeing. This liquor was produced from sukumo (composted Polygonum indigo leaves) |
[Ref.: #65373] |
Geographic location (country and/or sea, region) |
Date City, Hokkaido |
[Ref.: #65373] |
Country |
Japan |
[Ref.: #65373] |
Country ISO 3 Code |
JPN |
[Ref.: #65373] |
Continent |
Asia |
[Ref.: #65373] |
Geographic location |
42.4708°/140.8750° |
[Ref.: #65373] |
Enrichment culture |
indigo carmine-peptone/yeast extract/alkaline |
[Ref.: #65373] |
Enrichment culture composition |
(pH 10) consisting of 8.0 g peptone (Kyokuto), 3.0 g yeast extract (Merck), 2.0 g indigo carmine and 15.0 g agar in 1 l NaHCO3/Na2CO3 buffer (100 mM; pH 10) prepared using deionized water, and In-RCA medium (pH 10). The RCA contained 2.0 g indigo carmine in 1 l NaHCO3/Na2CO3 buffer (100 mM; pH 10) prepared using deionized water. A tenfold concentrated stock of each alkaline solution, which was used in each medium, was sterilized separately and then mixed with the primary autoclaved body of each |
[Ref.: #65373] |
Enrichment culture duration |
2 days |
[Ref.: #65373] |
Enrichment culture temperature |
27 ̊C |
[Ref.: #65373] |
Isolation/enrichment procedure |
An aliquot of the sample (0.5 ml) was diluted 10-4 10-5 times in series using 4.5 ml of physiological saline and inoculated into In-PYA medium. The inoculated media were incubated at 27°C for 2 days under an anaerobic N 2 gas-exchange jar. |
|
[Ref.: #67770] |
Sample type/isolated from |
Indigo fermentation liquor used for dyeing |
[Ref.: #67770] |
Geographic location (country and/or sea, region) |
Date City, Hokkaido |
[Ref.: #67770] |
Country |
Japan |
[Ref.: #67770] |
Country ISO 3 Code |
JPN |
[Ref.: #67770] |
Continent |
Asia |
* marker position based on {}
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Isolation sources categories |
#Host |
#Plants |
#Herbaceous plants (Grass,Crops) |
#Engineered |
#Industrial |
#Industrial production |
#Host Body-Site |
#Plant |
#Leaf (Phyllosphere) |
#Environmental |
#Aquatic |
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- References
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#67770 |
Japan Collection of Microorganism (JCM) ; Curators of the JCM;
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#69479 |
João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.:
MicrobeAtlas 1.0 beta
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- * These data were automatically processed and therefore are not curated
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