Strain identifier

BacDive ID: 158642

Type strain: Yes

Species: Blautia hominis

Strain Designation: KB1

Strain history: <- Jin-Woo Bae, Kyung Hee Univ.

NCBI tax ID(s): 2025493 (species)

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General

@ref: 65367

BacDive-ID: 158642

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, spore-forming, Gram-positive, ovoid-shaped, colony-forming

description: Blautia hominis KB1 is an anaerobe, spore-forming, Gram-positive bacterium that forms circular colonies and was isolated from faecal sample of a nonhospitalized 24-year-old male patient with diverticulitis.

NCBI tax id

  • NCBI tax id: 2025493
  • Matching level: species

strain history

@refhistory
67770J.-W. Bae; Kyung Hee Univ., South Korea; KB1.
67771<- Jin-Woo Bae, Kyung Hee Univ.

doi: 10.13145/bacdive158642.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Eubacteriales
  • family: Lachnospiraceae
  • genus: Blautia
  • species: Blautia hominis
  • full scientific name: Blautia hominis Shin et al. 2018

@ref: 65367

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Eubacteriales

family: Lachnospiraceae

genus: Blautia

species: Blautia hominis

strain designation: KB1

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotility
65367positive0.8-1.1 µm0.9-1 µmovoid-shapedno
67771no
67771positive

colony morphology

  • @ref: 65367
  • colony size: 1.2-2 mm
  • colony shape: circular
  • incubation period: 2 days
  • medium used: Gifu anaerobic medium

Culture and growth conditions

culture medium

  • @ref: 65367
  • name: Gifu anaerobic medium
  • growth: yes

culture temp

@refgrowthtypetemperaturerange
65367positivegrowth15-42
67770positivegrowth37mesophilic
67771positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
65367anaerobe
67771anaerobe

spore formation

  • @ref: 67771
  • type of spore: spore
  • spore formation: yes

observation

@refobservation
65367mainly occuring in pairs
65367Growth occurs in the presence of 20 % dehydrated bile

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6536727613amygdalin+builds acid from
6536718305arbutin+builds acid from
6536717057cellobiose-builds acid from
6536715963ribitol-builds acid from
6536717108D-arabinose+builds acid from
6536718333D-arabitol-builds acid from
6536715824D-fructose+builds acid from
6536728847D-fucose-builds acid from
6536712936D-galactose+builds acid from
6536717634D-glucose+builds acid from
6536762318D-lyxose-builds acid from
6536716899D-mannitol-builds acid from
6536716024D-mannose+builds acid from
6536716988D-ribose+builds acid from
6536717924D-sorbitol-builds acid from
6536716443D-tagatose-builds acid from
6536765327D-xylose+builds acid from
6536716813galactitol-builds acid from
6536717113erythritol-builds acid from
65367esculin ferric citrate-builds acid from
653675291gelatin-hydrolysis
6536728066gentiobiose+builds acid from
6536717754glycerol-builds acid from
6536728087glycogen-builds acid from
6536717268myo-inositol+builds acid from
6536715443inulin-builds acid from
6536730849L-arabinose+builds acid from
6536718403L-arabitol-builds acid from
6536718287L-fucose+builds acid from
6536762345L-rhamnose+builds acid from
6536717266L-sorbose-builds acid from
6536765328L-xylose-builds acid from
6536717716lactose+builds acid from
6536717306maltose+builds acid from
6536737684mannose+fermentation
653676731melezitose+builds acid from
6536728053melibiose+builds acid from
65367320061methyl alpha-D-glucopyranoside-builds acid from
6536743943methyl alpha-D-mannoside+builds acid from
6536774863methyl beta-D-xylopyranoside+builds acid from
65367506227N-acetylglucosamine-builds acid from
6536717632nitrate-reduction
65367potassium 2-dehydro-D-gluconate-builds acid from
65367potassium 5-dehydro-D-gluconate+builds acid from
6536732032potassium gluconate+builds acid from
6536716634raffinose+builds acid from
6536716634raffinose+fermentation
6536717814salicin+builds acid from
6536728017starch-builds acid from
6536717992sucrose+builds acid from
6536727082trehalose+builds acid from
6536732528turanose+builds acid from
6536717151xylitol-builds acid from

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitivesensitivity conc.is resistantresistance conc.
6536728971ampicillinyesyes10 µg (disc)
653676909metronidazoleyesyes25 µg (disc)
6536728001vancomycinyesyes30 µg (disc)
653673493cefoperazoneyesyes75 µg (disc)
6536717833gentamicinyesyes10 µg (disc)
653677507neomycinyesyes30 µg (disc)

metabolite production

  • @ref: 65367
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
65367alpha-galactosidase+3.2.1.22
65367alpha-glucosidase+3.2.1.20
65367beta-glucosidase+3.2.1.21
65367beta-glucuronidase+3.2.1.31
65367alpha-arabinosidase+3.2.1.55
65367N-acetyl-beta-glucosaminidase+3.2.1.52
65367alkaline phosphatase+3.1.3.1
65367arginine arylamidase+
65367leucyl glycin arylamidase+3.4.11.1
65367leucine arylamidase+3.4.11.1
65367tyrosine arylamidase+
65367glycin arylamidase+
65367catalase-1.11.1.6
65367cytochrome oxidase-1.9.3.1
65367urease-3.5.1.5
65367arginine dihydrolase-3.5.3.6
65367beta-galactosidase+3.2.1.23
653676-phospho-beta-galactosidase-3.2.1.85
65367glutamate decarboxylase-4.1.1.15
65367alpha-fucosidase-3.2.1.51
65367proline-arylamidase-3.4.11.5
65367pyroglutamic acid arylamidase-
65367phenylalanine arylamidase-
65367histidine arylamidase-
65367glutamyl-glutamate arylamidase-
65367serine arylamidase-

fatty acid profile

  • fatty acids

    @reffatty acidpercentageECL
    65367C12:01.6
    65367C13:1 at 12–13 and/or C14:0 aldehyde1.1
    65367C14:021.2
    65367C14:0 DMA2.5
    65367C15:2 and/or unknown equivalent chain-length1.415
    65367C16:0 aldehyde3.1
    65367C15:00.6
    65367C16:1ω7c1.4
    65367C16:024.4
    65367C16:1ω7c DMA3.6
    65367C16:0 DMA11.9
    65367C17:2 at 16.760 and/or C17:1ω9c1.5
    65367C17:1ω8c and/or C17:2 at 16.8011.4
    65367C18:1ω9c4.1
    65367C18:1ω9c and/or unknown3.318
    65367C18:01.7
    65367C18:1ω9c DMA6.4
    65367C18:1ω7c DMA6
    65367C18:0 DMA0.8
    65367C18:0 12-OH1.6
  • type of FA analysis: whole cell analysis
  • incubation medium: Gifu anaerobic medium
  • incubation temperature: 37
  • incubation time: 2
  • incubation_oxygen: anaerobic
  • software version: Sherlock 6.2
  • library/peak naming table: Moore library version 6.00
  • system: MIS MIDI
  • cutoff value:

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentenrichment cultureenrichment culture compositionenrichment culture durationenrichment culture temperatureisolation procedure
65367faecal sample of a nonhospitalized 24-year-old male patient with diverticulitisRepublic of KoreaKORAsiasterilized basal bicarbonate-buffered medium(per litre): 0.40 g KH2PO4 ; 0.53 g Na2HPO4 ; 0.30 g NH4Cl; 0.30 g NaCl; 0.10 g MgCl2 x 6H2O; 0.11 g CaCl2 x 2H2O; 4.00 g NaHCO3 ; 0.25 g Na2S; 1 ml acid trace element solution; 1 ml alkaline trace element solution; 1 ml vitamin solution; 7 ml sterilized rumen fluid (Bar Diamond) and 2.50 g porcine gastric mucin type III (Sigma)2 weeks371 g of the sample was inoculated in 30 ml of sterilized basal bicarbonate-buffered medium (pH 7.0, adjusted with 1 M HCl) supplemented with porcine mucin. After 2 weeks of incubation at 37°C, the inoculum was serially diluted, in 10- fold steps, in reduced PBS (Bioneer) and then 10-4 to 10-7 diluents were spread onto brain-heart infusion (BHI; BD) agar medium containing 0.25 % (w/v) porcine gastric mucin.
67770Fecal sample of a nonhospitalized 24-year-old male patient with diverticulitis in South Korea
67771From human feces of a nonhospitalized 24-year-old male patient with diverticulitisRepublic of KoreaKORAsia

isolation source categories

Cat1Cat2Cat3
#Host Body Product#Gastrointestinal tract#Feces (Stool)
#Host#Human#Male
#Infection#Inflammation

taxonmaps

  • @ref: 69479
  • File name: preview.99_22085.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_16;96_1360;97_1614;98_1963;99_22085&stattab=map
  • Last taxonomy: Blautia
  • 16S sequence: MG696224
  • Sequence Identity:
  • Total samples: 4226
  • soil counts: 10
  • aquatic counts: 50
  • animal counts: 4163
  • plant counts: 3

Sequence information

16S sequences

@refdescriptionaccessiondatabase
6536716S rRNA gene sequenceKY703632nuccore
6536716S rRNA gene sequenceMG696221nuccore
6536716S rRNA gene sequenceMG696222nuccore
6536716S rRNA gene sequenceMG696223nuccore
6536716S rRNA gene sequenceMG696224nuccore

Genome sequences

  • @ref: 67771
  • description: Blautia hominis KB1
  • accession: GCA_002270465
  • assembly level: contig
  • database: ncbi
  • NCBI tax ID: 2025493

GC content

  • @ref: 65367
  • GC-content: 46.3
  • method: genome sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
flagellatedno90.698yes
gram-positiveyes86.01no
anaerobicyes98.254yes
aerobicno96.963yes
halophileno84.802no
spore-formingyes77.646yes
glucose-utilyes92.594no
thermophileno97.248no
motileno80.37no
glucose-fermentyes70.67no

External links

@ref: 65367

culture collection no.: KCTC 15618, JCM 32276

literature

  • topic: Phylogeny
  • Pubmed-ID: 29458493
  • title: Blautia hominis sp. nov., isolated from human faeces.
  • authors: Shin NR, Kang W, Tak EJ, Hyun DW, Kim PS, Kim HS, Lee JY, Sung H, Whon TW, Bae JW
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.002623
  • year: 2018
  • mesh: Bacterial Typing Techniques, Base Composition, Clostridiales/*classification/genetics/isolation & purification, DNA, Bacterial/genetics, Fatty Acids/analysis, Feces/*microbiology, Fermentation, Humans, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sequence Analysis, DNA
  • topic2: Pathogenicity

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
65367Na-Ri Shin, Woorim Kang, Euon Jung Tak, Dong-Wook Hyun, Pil Soo Kim, Hyun Sik Kim, June-Young Lee, Hojun Sung, Tae Woong Whon, Jin-Woo BaeBlautia hominis sp. nov., isolated from human faeces10.1099/ijsem.0.002623IJSEM 68: 1059-1064 201829458493
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/