[Ref.: #65367] |
Sample type/isolated from |
faecal sample of a nonhospitalized 24-year-old male patient with diverticulitis |
[Ref.: #65367] |
Country |
Republic of Korea |
[Ref.: #65367] |
Country ISO 3 Code |
KOR |
[Ref.: #65367] |
Continent |
Asia |
[Ref.: #65367] |
Enrichment culture |
sterilized basal bicarbonate-buffered medium |
[Ref.: #65367] |
Enrichment culture composition |
(per litre): 0.40 g KH2PO4 ; 0.53 g Na2HPO4 ; 0.30 g NH4Cl; 0.30 g NaCl; 0.10 g MgCl2 x 6H2O; 0.11 g CaCl2 x 2H2O; 4.00 g NaHCO3 ; 0.25 g Na2S; 1 ml acid trace element solution; 1 ml alkaline trace element solution; 1 ml vitamin solution; 7 ml sterilized rumen fluid (Bar Diamond) and 2.50 g porcine gastric mucin type III (Sigma) |
[Ref.: #65367] |
Enrichment culture duration |
2 weeks |
[Ref.: #65367] |
Enrichment culture temperature |
37 ̊C |
[Ref.: #65367] |
Isolation/enrichment procedure |
1 g of the sample was inoculated in 30 ml of sterilized basal bicarbonate-buffered medium (pH 7.0, adjusted with 1 M HCl) supplemented with porcine mucin. After 2 weeks of incubation at 37°C, the inoculum was serially diluted, in 10- fold steps, in reduced PBS (Bioneer) and then 10-4 to 10-7 diluents were spread onto brain-heart infusion (BHI; BD) agar medium containing 0.25 % (w/v) porcine gastric mucin. |
|
[Ref.: #67770] |
Sample type/isolated from |
Fecal sample of a nonhospitalized 24-year-old male patient with diverticulitis in South Korea |
|
[Ref.: #67771] |
Sample type/isolated from |
From human feces of a nonhospitalized 24-year-old male patient with diverticulitis |
[Ref.: #67771] |
Country |
Republic of Korea |
[Ref.: #67771] |
Country ISO 3 Code |
KOR |
[Ref.: #67771] |
Continent |
Asia |
* marker position based on {}
|
|
Isolation sources categories |
#Host Body Product |
#Gastrointestinal tract |
#Feces (Stool) |
#Host |
#Human |
#Male |
#Infection |
#Inflammation |
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Availability in culture collections External links
[Ref.: #65367] |
Culture collection no. |
KCTC 15618, JCM 32276 |
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Literature: |
|
Topic |
Title |
Authors |
Journal |
DOI |
Year |
|
Phylogeny |
Blautia hominis sp. nov., isolated from human faeces. |
Shin NR, Kang W, Tak EJ, Hyun DW, Kim PS, Kim HS, Lee JY, Sung H, Whon TW, Bae JW |
Int J Syst Evol Microbiol |
10.1099/ijsem.0.002623 |
2018 |
* |
|
- References
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-
#65367 |
Na-Ri Shin, Woorim Kang, Euon Jung Tak, Dong-Wook Hyun, Pil Soo Kim, Hyun Sik Kim, June-Young Lee, Hojun Sung, Tae Woong Whon, Jin-Woo Bae:
Blautia hominis sp. nov., isolated from human faeces.
IJSEM 68:
1059 - 1064
2018 (
DOI 10.1099/ijsem.0.002623 , PubMed 29458493 )
|
-
#67770 |
Japan Collection of Microorganism (JCM) ; Curators of the JCM;
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-
#67771 |
Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;
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-
#69479 |
João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.:
MicrobeAtlas 1.0 beta
.
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- * These data were automatically processed and therefore are not curated
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