Strain identifier

BacDive ID: 158597

Type strain: Yes

Species: Mucilaginibacter amnicola

Strain Designation: TAPP7

Strain history: <- Wen-Ming Chen, National Kaohsiung Marine Univ..

NCBI tax ID(s): 1815558 (species)

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General

@ref: 65313

BacDive-ID: 158597

keywords: 16S sequence, Bacteria, aerobe, Gram-negative, rod-shaped, colony-forming

description: Mucilaginibacter amnicola TAPP7 is an aerobe, Gram-negative, rod-shaped bacterium that forms circular colonies and was isolated from fresh water sample.

NCBI tax id

  • NCBI tax id: 1815558
  • Matching level: species

strain history

  • @ref: 67771
  • history: <- Wen-Ming Chen, National Kaohsiung Marine Univ..

doi: 10.13145/bacdive158597.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacteroidota
  • domain: Bacteria
  • phylum: Bacteroidota
  • class: Sphingobacteriia
  • order: Sphingobacteriales
  • family: Sphingobacteriaceae
  • genus: Mucilaginibacter
  • species: Mucilaginibacter amnicola
  • full scientific name: Mucilaginibacter amnicola Chen et al. 2018

@ref: 65313

domain: Bacteria

phylum: Bacteroidetes

class: Sphingobacteriia

order: Sphingobacteriales

family: Sphingobacteriaceae

genus: Mucilaginibacter

species: Mucilaginibacter amnicola

strain designation: TAPP7

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotility
65313negative0.8-1.3 µm0.5-0.6 µmrod-shapedno
67771rod-shapedno
67771negative

colony morphology

@refcolony sizecolony colorcolony shapeincubation periodmedium used
653132-3.5 mmpinkcircular2 daysR2A
65313irregular

pigmentation

@refproductionname
65313noflexirubin-type pigments
65313yescarotinoid pigments

Culture and growth conditions

culture medium

@refnamegrowth
65313Reasoner's 2A agar (R2A)yes
65313tryptic soy agaryes
65313Nutrient agar (NA)yes
65313LB (Luria-Bertani) MEDIUMyes

culture temp

@refgrowthtypetemperaturerange
65313positivegrowth10-30
65313positiveoptimum15-20psychrophilic
67771positivegrowth25mesophilic

culture pH

@refabilitytypepHPH range
65313positivegrowth4-8acidophile
65313positiveoptimum6

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
65313aerobe
67771aerobe

halophily

@refsaltgrowthtested relationconcentration
65313NaClpositivegrowth0-1 %
65313NaClpositiveoptimum0.5 %

observation

@refobservation
65313Positive for poly-b-hydroxybutyrate accumulation.
67771quinones: MK-7

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
65313168082-dehydro-D-gluconate-builds acid from
65313645522-hydroxybutyrate-respiration
65313167632-oxobutanoate-respiration
65313309162-oxoglutarate-respiration
65313286442-oxopentanoate-respiration
65313620642,3-butanediol-respiration
65313370543-hydroxybutyrate-respiration
65313182404-hydroxy-L-proline-respiration
65313167244-hydroxybutyrate-respiration
65313181014-hydroxyphenylacetic acid-respiration
65313581435-dehydro-D-gluconate-builds acid from
6531330089acetate-respiration
6531317128adipate-assimilation
6531315963ribitol-respiration
6531340585alpha-cyclodextrin-respiration
6531317925alpha-D-glucose+respiration
6531336219alpha-lactose+respiration
6531327613amygdalin+builds acid from
6531322599arabinose-assimilation
6531318305arbutin+builds acid from
6531373706bromosuccinate-respiration
6531327689decanoate-assimilation
6531385146carboxymethylcellulose-hydrolysis
65313casein-hydrolysis
6531317057cellobiose+builds acid from
6531316383cis-aconitate-respiration
6531316947citrate-assimilation
6531316947citrate-respiration
65313crab shell chitin-hydrolysis
6531315963ribitol-builds acid from
6531315570D-alanine-respiration
6531317108D-arabinose+builds acid from
6531318333D-arabitol-builds acid from
6531318333D-arabitol-respiration
6531317057cellobiose+respiration
6531315824D-fructose+builds acid from
6531315824D-fructose+respiration
6531328847D-fucose-builds acid from
6531315895D-galactonic acid lactone-respiration
6531312936D-galactose+builds acid from
6531312936D-galactose+respiration
6531318024D-galacturonic acid-respiration
6531330612D-glucarate-respiration
653138391D-gluconate+respiration
6531317784D-glucosaminic acid+respiration
6531317634D-glucose+builds acid from
6531314314D-glucose 6-phosphate-respiration
6531315748D-glucuronate-respiration
6531362318D-lyxose+builds acid from
6531316899D-mannitol-builds acid from
6531316899D-mannitol-respiration
6531316024D-mannose+builds acid from
6531316024D-mannose+respiration
6531327605D-psicose+respiration
6531316988D-ribose-builds acid from
6531316523D-serine-respiration
6531317924D-sorbitol-builds acid from
6531317924D-sorbitol-respiration
6531316443D-tagatose+builds acid from
6531365327D-xylose+builds acid from
6531323652dextrin+respiration
6531317126DL-carnitine+respiration
6531324996lactate-respiration
6531316991dna+hydrolysis
6531316813galactitol-builds acid from
6531317113erythritol-builds acid from
6531317113erythritol-respiration
653134853esculin+builds acid from
653134853esculin+hydrolysis
6531316000ethanolamine-respiration
6531315740formate-respiration
6531316865gamma-aminobutyric acid-respiration
653135291gelatin-hydrolysis
6531328066gentiobiose+builds acid from
6531328066gentiobiose+respiration
6531324265gluconate-assimilation
6531324265gluconate-builds acid from
6531317234glucose-builds acid from
6531317234glucose+assimilation
6531329042glucose 1-phosphate-respiration
6531332323glucuronamide-respiration
6531317754glycerol-builds acid from
6531317754glycerol-respiration
6531314336glycerol 1-phosphate-respiration
6531328087glycogen-builds acid from
6531328087glycogen-respiration
6531373804glycyl L-aspartic acid-respiration
6531317596inosine-respiration
6531317268myo-inositol-builds acid from
6531315443inulin+builds acid from
6531317240itaconate-respiration
6531321217L-alaninamide+respiration
6531316977L-alanine-respiration
6531373786L-alanylglycine-respiration
6531330849L-arabinose-builds acid from
6531330849L-arabinose+respiration
6531318403L-arabitol-builds acid from
6531317196L-asparagine-respiration
6531329991L-aspartate-respiration
6531318287L-fucose-builds acid from
6531318287L-fucose-respiration
6531329985L-glutamate+respiration
6531315971L-histidine-respiration
6531315603L-leucine-respiration
6531315729L-ornithine-respiration
6531317295L-phenylalanine-respiration
6531317203L-proline+respiration
6531318183L-pyroglutamic acid-respiration
6531362345L-rhamnose-builds acid from
6531362345L-rhamnose-respiration
6531317115L-serine-respiration
6531317266L-sorbose+builds acid from
6531316857L-threonine+respiration
6531365328L-xylose-builds acid from
6531317716lactose+builds acid from
653136359lactulose+respiration
6531325115malate-assimilation
6531315792malonate-respiration
6531317306maltose+assimilation
6531317306maltose+builds acid from
6531317306maltose+respiration
6531329864mannitol-assimilation
6531337684mannose+assimilation
653136731melezitose+builds acid from
6531328053melibiose+builds acid from
6531328053melibiose+respiration
6531343943methyl alpha-D-mannoside+builds acid from
65313320055methyl beta-D-glucopyranoside+respiration
6531374863methyl beta-D-xylopyranoside+builds acid from
6531337657methyl D-glucoside+builds acid from
6531351850methyl pyruvate+respiration
6531375146monomethyl succinate-respiration
6531317268myo-inositol-respiration
6531328037N-acetylgalactosamine-respiration
65313506227N-acetylglucosamine+assimilation
65313506227N-acetylglucosamine+builds acid from
65313506227N-acetylglucosamine+respiration
6531373784glycyl-l-glutamate-respiration
6531317632nitrate-reduction
6531318401phenylacetate-assimilation
6531350048phenylethylamine-respiration
6531317272propionate-respiration
6531317148putrescine-respiration
6531326490quinate-respiration
6531316634raffinose+builds acid from
6531316634raffinose+respiration
6531317814salicin+builds acid from
6531341865sebacic acid-respiration
6531328017starch-hydrolysis
6531328017starch+builds acid from
65313143136succinamate-respiration
6531330031succinate-respiration
6531317992sucrose+builds acid from
6531317992sucrose+respiration
6531317748thymidine-respiration
6531327082trehalose+builds acid from
6531327082trehalose+respiration
6531332528turanose+builds acid from
6531332528turanose+respiration
6531353424tween 20-hydrolysis
6531353423tween 40-hydrolysis
6531353423tween 40+respiration
6531353425tween 60-hydrolysis
6531353426tween 80-hydrolysis
6531353426tween 80-respiration
6531316704uridine-respiration
6531327248urocanic acid-respiration
6531317151xylitol-builds acid from
6531317151xylitol-respiration

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitivesensitivity conc.is resistantresistance conc.group ID
6531328077rifampicinyesyes5 µg (disc)
6531317076streptomycinyesyes10 µg (disc)
6531317698chloramphenicolyesyes30 µg (disc)
65313100147nalidixic acidyesyes30 µg (disc)
6531328368novobiocinyesyes30 µg (disc)
6531327902tetracyclineyesyes30 µg (disc)
6531318208penicillin gyesyes10 Unit (disc)
6531317833gentamicinyesyes10 µg (disc)
6531328971ampicillinyesyes11 µg (disc)
653136104kanamycinyesyes30 µg (disc)
6531345924trimethoprimyesyes1.25 µg (disc)25
65313102130sulfamerazineyesyes23.75 µg (disc)25

metabolite production

  • @ref: 65313
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
65313cytochrome oxidase+1.9.3.1
65313beta-galactosidase+3.2.1.23
65313alkaline phosphatase+3.1.3.1
65313esterase (C 4)+
65313esterase Lipase (C 8)+
65313leucine arylamidase+3.4.11.1
65313valine arylamidase+
65313cystine arylamidase+3.4.11.3
65313acid phosphatase+3.1.3.2
65313naphthol-AS-BI-phosphohydrolase+
65313alpha-galactosidase+3.2.1.22
65313beta-glucuronidase+3.2.1.31
65313alpha-glucosidase+3.2.1.20
65313beta-glucosidase+3.2.1.21
65313N-acetyl-beta-glucosaminidase+3.2.1.52
65313alpha-mannosidase+3.2.1.24
65313alpha-fucosidase+3.2.1.51
65313catalase-1.11.1.6
65313urease-3.5.1.5
65313arginine dihydrolase-3.5.3.6
65313lipase (C 14)-
65313trypsin-3.4.21.4
65313alpha-chymotrypsin-3.4.21.1

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    65313C12:01.8
    65313C14:01.2
    65313C16:08.2
    65313C18:03.1
    65313C14:0 anteiso3.8
    65313C15:0 iso17.2
    65313C17:0 iso1.4
    65313C15:1ω6c2.1
    65313C16:1ω5c5.2
    65313C17:1ω8c1.4
    65313C18:1ω9c2.4
    65313C15:0 2OH1.9
    65313C16:0 3OH2.1
    65313C17:0 3OH1.2
    65313C17:0 iso 3OH6.1
    65313C16:1ω7c and/or C16:1ω6c35.8
    65313C16:0 10-methyl and/or iso-C17:1ω9c1.8
  • type of FA analysis: whole cell analysis
  • incubation medium: R2A
  • agar/liquid: agar
  • incubation temperature: 25
  • incubation time: 3
  • software version: Sherlock 6.0
  • library/peak naming table: RTSBA6.00
  • system: MIS MIDI
  • cutoff value:

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitudeenrichment cultureenrichment culture durationenrichment culture temperature
65313fresh water sampleWanan Creek in the vicinity of Pingtung CountyTaiwanTWNAsia22.6225120.647R2A agar3 days25
67771From fresh water, riverWanan Creek, the vicinity of Pingtung CountyTaiwanTWNAsia

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Aquatic
  • Cat3: #Freshwater

Sequence information

16S sequences

  • @ref: 65313
  • description: 16S rRNA gene sequence
  • accession: LT223124
  • database: nuccore

GC content

@refGC-contentmethod
6531345.6high performance liquid chromatography (HPLC)
6777145.6

External links

@ref: 65313

culture collection no.: BCRC 80976, LMG 29556, KCTC 52238

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny29219806Mucilaginibacter amnicola sp. nov., isolated from a freshwater creek.Chen WM, Hsieh TY, Sheu SYInt J Syst Evol Microbiol10.1099/ijsem.0.0025182017Bacterial Typing Techniques, Bacteroidetes/*classification/genetics/isolation & purification, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Hydroxybutyrates/metabolism, Phospholipids/chemistry, *Phylogeny, Polyesters/metabolism, RNA, Ribosomal, 16S/genetics, Rivers/*microbiology, Sequence Analysis, DNA, Spermidine/chemistry, Taiwan, Vitamin K 2/analogs & derivatives/chemistry, *Water MicrobiologyMetabolism
Phylogeny31350573Mucilaginibacter terrigena sp. nov. sp., A Novel Member of the Family Sphingobacteriaceae.Ten LN, Jeon NY, Li W, Cho YJ, Kim MK, Lee SY, Rooney AP, Jung HYCurr Microbiol10.1007/s00284-019-01748-y2019Bacteroidetes/chemistry/*classification/*physiology, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Genome, Bacterial/genetics, Nucleic Acid Hybridization, Phosphatidylethanolamines/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sequence Analysis, DNA, Soil Microbiology, Species Specificity, Vitamin K 2/analogs & derivatives/chemistryGenetics

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
65313Wen-Ming Chen, Tsai-Ying Hsieh, Shih-Yi SheuMucilaginibacter amnicola sp. nov., isolated from a freshwater creek10.1099/ijsem.0.002518IJSEM 68: 394-401 201829219806
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc