Strain identifier

BacDive ID: 158589

Type strain: Yes

Species: Aestuariicoccus marinus

Strain Designation: NAP41

Strain history: C. O. Jeon; Chung-Ang Univ., South Korea; NAP41.

NCBI tax ID(s): 1614698 (species)

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General

@ref: 65303

BacDive-ID: 158589

keywords: 16S sequence, Bacteria, aerobe, Gram-negative, coccus-shaped

description: Aestuariicoccus marinus NAP41 is an aerobe, Gram-negative, coccus-shaped bacterium that was isolated from tidal flat sediment sample.

NCBI tax id

  • NCBI tax id: 1614698
  • Matching level: species

strain history

  • @ref: 67770
  • history: C. O. Jeon; Chung-Ang Univ., South Korea; NAP41.

doi: 10.13145/bacdive158589.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Rhodobacterales
  • family: Roseobacteraceae
  • genus: Aestuariicoccus
  • species: Aestuariicoccus marinus
  • full scientific name: Aestuariicoccus marinus Feng et al. 2018

@ref: 65303

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Rhodobacterales

family: Rhodobacteraceae

genus: Aestuariicoccus

species: Aestuariicoccus marinus

strain designation: NAP41

type strain: yes

Morphology

cell morphology

  • @ref: 65303
  • gram stain: negative
  • cell length: 0.9-1.3 µm
  • cell shape: coccus-shaped
  • motility: no

Culture and growth conditions

culture medium

@refnamegrowthcomposition
65303Marine agar (MA)yes
65303Reasoner's 2A agar (R2A)yes
65303LB (Luria-Bertani) MEDIUMyes
65303tryptic soy agaryes2 % NaCl

culture temp

@refgrowthtypetemperaturerange
65303positivegrowth15-40
65303positiveoptimum37mesophilic
67770positivegrowth30mesophilic

culture pH

@refabilitytypepHPH range
65303positivegrowth6.5-9alkaliphile
65303positiveoptimum7-7.5

Physiology and metabolism

oxygen tolerance

  • @ref: 65303
  • oxygen tolerance: aerobe

halophily

@refsaltgrowthtested relationconcentration
65303NaClpositivegrowth0.5-12 %(w/v)
65303NaClpositiveoptimum2 %(w/v)

observation

  • @ref: 67770
  • observation: quinones: Q-10

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
65303309162-oxoglutarate+respiration
65303370543-hydroxybutyrate+respiration
6530317128adipate-assimilation
6530340585alpha-cyclodextrin+respiration
6530327689decanoate-assimilation
65303casein-hydrolysis
6530316383cis-aconitate+respiration
6530318333D-arabitol+respiration
6530317057cellobiose+respiration
6530315824D-fructose+respiration
6530315895D-galactonic acid lactone+respiration
6530317634D-glucose+assimilation
6530316899D-mannitol+assimilation
6530316899D-mannitol+respiration
6530316024D-mannose+assimilation
6530316024D-mannose+respiration
6530327605D-psicose+respiration
6530317924D-sorbitol+respiration
6530323652dextrin+respiration
653034853esculin+hydrolysis
6530328066gentiobiose+respiration
6530316977L-alanine+respiration
6530330849L-arabinose+assimilation
6530317196L-asparagine+respiration
6530315729L-ornithine+respiration
6530317115L-serine+respiration
6530325115malate+assimilation
6530317306maltose+assimilation
6530375146monomethyl succinate+respiration
65303506227N-acetylglucosamine-assimilation
6530317632nitrate+reduction
6530318401phenylacetate-assimilation
6530332032potassium gluconate-assimilation
6530353258sodium citrate-assimilation
6530328017starch-hydrolysis
65303143136succinamate+respiration
6530330031succinate+respiration
6530317992sucrose+respiration
6530317748thymidine+respiration
6530353424tween 20-hydrolysis
6530353426tween 80+hydrolysis
6530318186tyrosine+hydrolysis

enzymes

@refvalueactivityec
65303cytochrome oxidase+1.9.3.1
65303catalase+1.11.1.6
65303alkaline phosphatase+3.1.3.1
65303esterase (C 4)+
65303esterase Lipase (C 8)+
65303leucine arylamidase+3.4.11.1
65303valine arylamidase+
65303acid phosphatase+3.1.3.2
65303naphthol-AS-BI-phosphohydrolase+
65303alpha-glucosidase+3.2.1.20
65303lipase (C 14)-
65303cystine arylamidase-3.4.11.3
65303trypsin-3.4.21.4
65303alpha-chymotrypsin-3.4.21.1
65303alpha-galactosidase-3.2.1.22
65303beta-galactosidase-3.2.1.23
65303beta-glucuronidase-3.2.1.31
65303N-acetyl-beta-glucosaminidase-3.2.1.52
65303alpha-mannosidase-3.2.1.24
65303alpha-fucosidase-3.2.1.51
65303beta-glucosidase-3.2.1.21

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    65303C16:03.8
    65303C17:00.6
    65303C18:08.2
    65303C18:1ω7c 11-methyl3.7
    65303C12:1 3OH1.6
    65303C14:0 anteiso1.1
    65303C15:0 anteiso0.7
    65303C18:1ω7c and/or C18:1ω6c78.6
  • type of FA analysis: whole cell analysis
  • incubation medium: marine broth
  • incubation temperature: 37
  • software version: Sherlock 6.0B
  • library/peak naming table: TSBA6
  • system: MIS MIDI
  • cutoff value:

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitudeenrichment cultureenrichment culture durationenrichment culture temperatureisolation procedure
65303tidal flat sediment sampleTaean coastal areaRepublic of KoreaKORAsia36.8141126.186marine agar 22163 days25serially diluted with artificial seawater (20 g NaCl, 2.9 g MgSO4, 4.53 g MgCl2 x 6H2O, 0.64 g KCl, 1.75 g CaCl2 x 2H2O per litre), spread onto marine agar 2216 and incubated at 25°C for 3 days under aerobic conditions.
67770Tidal flat sediment sample obtained from the Taean coastal areaRepublic of KoreaKORAsia

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Sediment
#Environmental#Terrestrial#Tidal flat
#Environmental#Aquatic#Marine

taxonmaps

  • @ref: 69479
  • File name: preview.99_20570.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_19;96_826;97_12623;98_15537;99_20570&stattab=map
  • Last taxonomy: Aestuariicoccus marinus subclade
  • 16S sequence: MF113251
  • Sequence Identity:
  • Total samples: 228
  • soil counts: 9
  • aquatic counts: 190
  • animal counts: 29

Sequence information

16S sequences

  • @ref: 65303
  • description: 16S rRNA gene sequence
  • accession: MF113251
  • database: nuccore

GC content

@refGC-contentmethod
6530356real time PCR (RTD-PCR)
6777056thermal denaturation, midpoint method (Tm)

External links

@ref: 65303

culture collection no.: KACC 18431, JCM 30739

literature

  • topic: Phylogeny
  • Pubmed-ID: 29139347
  • title: Aestuariicoccus marinus gen. nov., sp. nov., isolated from sea-tidal flat sediment.
  • authors: Feng T, Jeong SE, Kim KH, Park HY, Jeon CO
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.002494
  • year: 2017
  • mesh: Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Geologic Sediments/*microbiology, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Rhodobacteraceae/*classification/genetics/isolation & purification, Seawater/*microbiology, Sequence Analysis, DNA, Ubiquinone/chemistry
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
65303Tingye Feng, Sang Eun Jeong, Kyung Hyun Kim, Hye Yoon Park, Che Ok JeonAestuariicoccus marinus gen. nov., sp. nov., isolated from sea-tidal flat sediment10.1099/ijsem.0.002494IJSEM 68: 260-265 201829139347
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/