Strain identifier

BacDive ID: 158586

Type strain: Yes

Species: Microbacterium deserti

Strain history: <- Wael Nabil Hozzein, King Saud Univ.

NCBI tax ID(s): 2053190 (species)

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For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 65299

BacDive-ID: 158586

keywords: 16S sequence, Bacteria, aerobe, Gram-positive, rod-shaped, colony-forming

description: Microbacterium deserti CPCC 204619 is an aerobe, Gram-positive, rod-shaped bacterium that forms circular colonies and was isolated from desert soil sample.

NCBI tax id

  • NCBI tax id: 2053190
  • Matching level: species

strain history

  • @ref: 67771
  • history: <- Wael Nabil Hozzein, King Saud Univ.

doi: 10.13145/bacdive158586.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micrococcales
  • family: Microbacteriaceae
  • genus: Microbacterium
  • species: Microbacterium deserti
  • full scientific name: Microbacterium deserti Yang et al. 2018

@ref: 65299

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Micrococcales

family: Microbacteriaceae

genus: Microbacterium

species: Microbacterium deserti

type strain: yes

Morphology

cell morphology

  • @ref: 65299
  • gram stain: positive
  • cell length: 0.9-1.1 µm
  • cell width: 0.4-0.5 µm
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 65299
  • colony color: lemon yellos
  • colony shape: circular
  • incubation period: 5 days
  • medium used: R2A

Culture and growth conditions

culture medium

  • @ref: 65299
  • name: Reasoner's 2A agar (R2A)
  • growth: yes

culture temp

@refgrowthtypetemperaturerange
65299positivegrowth4-45
65299positiveoptimum28mesophilic
67771positivegrowth28mesophilic

culture pH

@refabilitytypepHPH range
65299positivegrowth5-10alkaliphile
65299positiveoptimum7

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
65299aerobe
67771aerobe

spore formation

  • @ref: 65299
  • spore formation: no

halophily

  • @ref: 65299
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-8 %(w/v)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6529915588D-malate+assimilation
6529916651(S)-lactate+assimilation
6529915589L-malate+assimilation
65299309162-oxoglutarate+assimilation
65299181014-hydroxyphenylacetic acid+assimilation
6529930089acetate+assimilation
6529917128adipate-assimilation
6529917925alpha-D-glucose+assimilation
6529927689decanoate-assimilation
6529917057cellobiose+assimilation
6529962968cellulose-hydrolysis
6529915824D-fructose+assimilation
6529915748D-glucuronate+assimilation
6529916899D-mannitol+assimilation
6529916024D-mannose+assimilation
6529917924D-sorbitol+assimilation
6529923652dextrin+assimilation
652994853esculin+hydrolysis
652995291gelatin+hydrolysis
6529928066gentiobiose+assimilation
6529924265gluconate+assimilation
6529917234glucose+assimilation
6529917596inosine+assimilation
6529918287L-fucose+assimilation
6529962345L-rhamnose+assimilation
6529925115malate+assimilation
6529917306maltose-assimilation
6529929864mannitol+assimilation
6529937684mannose+assimilation
6529963153N-acetyl-D-mannosamine+assimilation
65299506227N-acetylglucosamine+assimilation
6529917632nitrate+reduction
6529917272propionate+assimilation
6529917814salicin+assimilation
6529953258sodium citrate-assimilation
6529928017starch-hydrolysis
6529927082trehalose+assimilation
6529932528turanose+assimilation
6529953424tween 20-hydrolysis
6529953423tween 40+assimilation
6529953423tween 40+hydrolysis
6529953425tween 60+hydrolysis
6529953426tween 80-hydrolysis

metabolite production

  • @ref: 65299
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
65299catalase+1.11.1.6
65299cytochrome oxidase+1.9.3.1
65299acid phosphatase+3.1.3.2
65299esterase (C 4)+
65299esterase Lipase (C 8)+
65299alpha-glucosidase+3.2.1.20
65299leucine arylamidase+3.4.11.1
65299naphthol-AS-BI-phosphohydrolase+
65299valine arylamidase+
65299urease-3.5.1.5
65299alkaline phosphatase-3.1.3.1
65299alpha-chymotrypsin-3.4.21.1
65299cystine arylamidase-3.4.11.3
65299beta-D-fucosidase-3.2.1.38
65299alpha-galactosidase-3.2.1.22
65299beta-galactosidase-3.2.1.23
65299N-acetyl-beta-glucosaminidase-3.2.1.52
65299beta-glucosidase-3.2.1.21
65299beta-glucuronidase-3.2.1.31
65299lipase (C 14)+
65299alpha-mannosidase-3.2.1.24
65299trypsin-3.4.21.4
65299arginine dihydrolase-3.5.3.6

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    65299C14:0 iso0.3
    65299C15:0 iso0.7
    65299C15:0 anteiso49.5
    65299C16:0 iso7.6
    65299C16:01
    65299C16:0 10-methyl and/or iso-C17:1 ω9c0.5
    65299C17:0 iso0.6
    65299C17:0 anteiso38.8
    65299C17:00.2
  • type of FA analysis: whole cell analysis
  • incubation medium: tryptic soy agar
  • incubation temperature: 28
  • incubation time: 7
  • incubation pH: 7
  • software version: Sherlock 6.1
  • library/peak naming table: TSBA6
  • system: MIS MIDI

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentenrichment cultureenrichment culture compositionenrichment culture durationenrichment culture temperature
65299desert soil samplearid desert in the Al-Jouf regionSaudi ArabiaSAUAsiaR2ApH 75 days28
67771From desertRiyadhSaudi ArabiaSAUAsia

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Desert
#Environmental#Terrestrial#Soil

taxonmaps

  • @ref: 69479
  • File name: preview.99_4935.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_9;96_417;97_2954;98_3694;99_4935&stattab=map
  • Last taxonomy: Microbacterium lacusdiani
  • 16S sequence: MF399040
  • Sequence Identity:
  • Total samples: 223
  • soil counts: 25
  • aquatic counts: 86
  • animal counts: 70
  • plant counts: 42

Sequence information

16S sequences

  • @ref: 65299
  • description: 16S rRNA gene sequence
  • accession: MF399040
  • database: nuccore

GC content

  • @ref: 65299
  • GC-content: 73.6
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 65299

culture collection no.: CPCC 204619, KCTC 39991, SYSU D8014

literature

  • topic: Phylogeny
  • Pubmed-ID: 29125456
  • title: Microbacterium album sp. nov. and Microbacterium deserti sp. nov., two halotolerant actinobacteria isolated from desert soil.
  • authors: Yang ZW, Salam N, Mohany M, Chinnathambi A, Alharbi SA, Xiao M, Hozzein WN, Li WJ
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.002485
  • year: 2017
  • mesh: Actinobacteria/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, *Desert Climate, Fatty Acids/chemistry, Glycolipids/chemistry, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Saudi Arabia, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/chemistry
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
65299Zi-Wen Yang, Nimaichand Salam, Mohamed Mohany, Arunachalam Chinnathambi, Sulaiman Ali Alharbi, Min Xiao, Wael N. Hozzein, Wen-Jun LiMicrobacterium album sp. nov. and Microbacterium deserti sp. nov., two halotolerant actinobacteria isolated from desert soil10.1099/ijsem.0.002485IJSEM 68: 217-222 201829125456
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/