Strain identifier

BacDive ID: 158538

Type strain: Yes

Species: Mangrovitalea sediminis

Strain Designation: M11-4

Strain history: T. Yun; Xiamen Univ., China; M11-4.

NCBI tax ID(s): 1982043 (species)

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General

@ref: 65226

BacDive-ID: 158538

keywords: genome sequence, 16S sequence, Bacteria, facultative anaerobe, Gram-negative, motile, rod-shaped

description: Mangrovitalea sediminis M11-4 is a facultative anaerobe, Gram-negative, motile bacterium that was isolated from mangrove sediment sample.

NCBI tax id

  • NCBI tax id: 1982043
  • Matching level: species

strain history

  • @ref: 67770
  • history: T. Yun; Xiamen Univ., China; M11-4.

doi: 10.13145/bacdive158538.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Alteromonadales
  • family: Alteromonadaceae
  • genus: Mangrovitalea
  • species: Mangrovitalea sediminis
  • full scientific name: Mangrovitalea sediminis Liao et al. 2017

@ref: 65226

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Alteromonadales

family: Alteromonadaceae

genus: Mangrovitalea

species: Mangrovitalea sediminis

strain designation: M11-4

type strain: yes

Morphology

cell morphology

  • @ref: 65226
  • gram stain: negative
  • cell length: 2.6-4.3 µm
  • cell width: 0.6-0.8 µm
  • cell shape: rod-shaped
  • motility: yes
  • flagellum arrangement: monotrichous, polar

colony morphology

  • @ref: 65226
  • colony size: 0.5-2 mm
  • colony color: white
  • incubation period: 3 days
  • medium used: 2216 E agar

Culture and growth conditions

culture medium

  • @ref: 65226
  • name: 2216 E agar
  • growth: yes
  • composition: with 5.0 g peptone, 1.0 g yeast extract, KH2PO4 0.0744 g, Fe2(SO4 )3 0.002 g, agar 12 g, aged seawater 750 ml, distilled water 250 ml, pH 7.0

culture temp

@refgrowthtypetemperaturerange
65226positivegrowth10-40
65226positiveoptimum30mesophilic
67770positivegrowth30mesophilic

culture pH

@refabilitytypepH
65226positivegrowth5-8
65226positiveoptimum6
65226nogrowth5

Physiology and metabolism

oxygen tolerance

  • @ref: 65226
  • oxygen tolerance: facultative anaerobe

halophily

@refsaltgrowthtested relationconcentration
65226NaClpositivegrowth0.5-6 %(w/v)
65226NaClpositiveoptimum2-3 %(w/v)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6522617128adipate-assimilation
6522627613amygdalin-assimilation
6522622599arabinose-assimilation
6522627689decanoate-assimilation
65226casein-hydrolysis
65226casein hydrolysate-hydrolysis
6522617029chitin-hydrolysis
6522616947citrate-assimilation
6522617634D-glucose-assimilation
6522617634D-glucose+assimilation
6522617634D-glucose+fermentation
6522616899D-mannitol-assimilation
6522616024D-mannose+assimilation
652264853esculin-hydrolysis
652265291gelatin-hydrolysis
6522617234glucose+assimilation
6522630849L-arabinose-assimilation
6522616467L-arginine-assimilation
6522662345L-rhamnose-assimilation
6522625115malate-assimilation
6522617306maltose+assimilation
6522629864mannitol-assimilation
6522628053melibiose-assimilation
6522617268myo-inositol-assimilation
65226506227N-acetylglucosamine-assimilation
6522617632nitrate-reduction
6522618401phenylacetate-assimilation
6522632032potassium gluconate-assimilation
6522626546rhamnose-assimilation
6522653258sodium citrate-assimilation
6522630911sorbitol-assimilation
6522628017starch-hydrolysis
6522617992sucrose-assimilation
6522653424tween 20+hydrolysis
6522653423tween 40+hydrolysis
6522653425tween 60+hydrolysis
6522653426tween 80+hydrolysis
6522616199urea-hydrolysis
6522618222xylose-hydrolysis

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitivesensitivity conc.is resistantresistance conc.
6522628077rifampicinyesyes5 µg (disc)
6522628971ampicillinyesyes10 µg (disc)
6522617833gentamicinyesyes10 µg (disc)
6522617334penicillinyesyes10 µg (disc)
6522617076streptomycinyesyes10 µg (disc)
6522648923erythromycinyesyes15 µg (disc)
652263547cephradineyesyes30 µg (disc)
652263534cephalexinyesyes30 µg (disc)
65226474053cefazolinyesyes30 µg (disc)
6522650845doxycyclineyesyes30 µg (disc)
652266104kanamycinyesyes30 µg (disc)
6522650694minocyclineyesyes30 µg (disc)
65226100246norfloxacinyesyes30 µg (disc)
652267731ofloxacinyesyes30 µg (disc)
652263393carbenicillinyesyes100 µg (disc)
65226102484sulfisoxazoleyesyes300 µg (disc)
652267809oxacillinyesyes1 µg (disc)
652263745clindamycinyesyes2 µg (disc)
6522628368novobiocinyesyes5 µg (disc)
6522637943colistinyesyes10 µg (disc)
6522627902tetracyclineyesyes30 µg (disc)

metabolite production

@refChebi-IDmetaboliteproduction
6522615688acetoinyes
6522616136hydrogen sulfideno
6522635581indoleno

enzymes

@refvalueactivityec
65226catalase+1.11.1.6
65226cytochrome oxidase+1.9.3.1
65226esterase (C 4)+
65226esterase Lipase (C 8)+
65226lipase (C 14)+
65226leucine aminopeptidase+3.4.1.1
65226valine aminopeptidase+
65226acid phosphatase+3.1.3.2
65226alpha-glucosidase+3.2.1.20
65226cystine aminopeptidase+3.4.11.3
65226beta-glucosidase+/-3.2.1.21
65226beta-glucuronidase+3.2.1.31
65226alkaline phosphatase-3.1.3.1
65226alpha-chymotrypsin-3.4.21.1
65226trypsin-3.4.21.4
65226naphthol-AS-BI-phosphohydrolase-
65226alpha-galactosidase-3.2.1.22
65226N-acetyl-beta-glucosaminidase-3.2.1.52
65226alpha-fucosidase-3.2.1.51
65226alpha-mannosidase-3.2.1.24
65226urease-3.5.1.5
65226tryptophan deaminase-4.1.99.1
65226gelatinase-
65226lysine decarboxylase-4.1.1.18
65226arginine dihydrolase+3.5.3.6
65226beta-galactosidase-3.2.1.23
65226ornithine decarboxylase-4.1.1.17

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    65226C12:03.7
    65226C14:03.8
    65226C16:032.3
    65226C11:0 2OH1.8
    65226C12:0 3OH2.5
    65226C12:0 iso1.7
    65226C11:0 anteiso1.3
    65226C13:0 anteiso1.5
    65226C14:0 anteiso13
    65226C16:0 N alcohol14.8
    65226C16:1ω11c2.8
    65226C14:0 3-OH and/or iso-C16:11.7
    65226C16:1ω7c and/or C16:1ω6c8.2
    65226C18:1ω7c and/or C18:1ω6c2.7
    65226iso-C17:1ω9c and/or C16:0 10-methyl13.3
  • type of FA analysis: whole cell analysis
  • incubation medium: 2216 E agar
  • agar/liquid: agar
  • incubation temperature: 30
  • incubation time: 2
  • software version: Sherlock 6.0
  • library/peak naming table: TSBA 6.0
  • system: MIS MIDI
  • cutoff value:

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitudeenrichment cultureenrichment culture compositionenrichment culture durationenrichment culture temperatureisolation procedure
65226mangrove sediment sampleYunxiao Mangrove National Nature Reserve, Zhangjiang estuaryChinaCHNAsia24.8666117.583MM2 medium100 ml MM2 medium with [(NH4 )2SO4 0.23 g, K2HPO4 0.0136 g, FeSO4 0.00028 g, aged seawater 750 ml, distilled water 250 ml, pH 7.0-7.2] with additional benzo[a]pyrene (10ppm)4 weeks28incubated at 28°C and 150 r.p.m. in the dark for four weeks; and a 40 ml aliquot of the enriched culture was transferred into fresh benzo[a]pyrene -MM2 medium for the second enrichment. This step was repeated eight times, then serial dilutions of 10^-2, 10^-3, 10^-4 were selected and a 100 µl portion was spread on MM2 agar (1000 ml MM2 medium with 12 g agar) plate with additional benzo[a]pyrene [10 µl benzo[a]pyrene (10ppm) was spread on every plate]. The plates were aerobically incubated in the
67770Mangrove sediment in Yunxiao Mangrove National Nature ReserveChinaCHNAsia

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Mangrove
#Environmental#Terrestrial#Sediment
#Host#Plants#Tree

Sequence information

16S sequences

  • @ref: 65226
  • description: 16S rRNA gene sequence
  • accession: MF034046
  • database: nuccore

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Mangrovitalea sediminis M11-42788500092draftimg1982043
67770Mangrovitalea sediminis M11-4GCA_002514725contigncbi1982043

GC content

  • @ref: 67770
  • GC-content: 58.2
  • method: genome sequence analysis

External links

@ref: 65226

culture collection no.: MCCC 1K03312, JCM 32104

literature

  • topic: Phylogeny
  • Pubmed-ID: 29043950
  • title: Mangrovitalea sediminis gen. nov., sp. nov., a member of the family Alteromonadaceae isolated from mangrove sediment.
  • authors: Liao H, Li Y, Guo X, Lin X, Lai Q, Xu H, Zheng T, Tian Y
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.002433
  • year: 2017
  • mesh: Alteromonadaceae/*classification/genetics/isolation & purification, Avicennia/*microbiology, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Geologic Sediments/*microbiology, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Ubiquinone/chemistry
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
65226Hu Liao, Yuqian Li, Xintong Guo, Xiaolan Lin, Qiliang Lai, Hong Xu, Tianling Zheng, Yun TianMangrovitalea sediminis gen. nov., sp. nov., a member of the family Alteromonadaceae isolated from mangrove sediment10.1099/ijsem.0.002433IJSEM 67: 5172-5178 201729043950
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/