[Ref.: #65226] |
Sample type/isolated from |
mangrove sediment sample |
[Ref.: #65226] |
Geographic location (country and/or sea, region) |
Yunxiao Mangrove National Nature Reserve, Zhangjiang estuary |
[Ref.: #65226] |
Country |
China |
[Ref.: #65226] |
Country ISO 3 Code |
CHN |
[Ref.: #65226] |
Continent |
Asia |
[Ref.: #65226] |
Geographic location |
24.8666°/117.5830° |
[Ref.: #65226] |
Enrichment culture |
MM2 medium |
[Ref.: #65226] |
Enrichment culture composition |
100 ml MM2 medium with [(NH4 )2SO4 0.23 g, K2HPO4 0.0136 g, FeSO4 0.00028 g, aged seawater 750 ml, distilled water 250 ml, pH 7.0-7.2] with additional benzo[a]pyrene (10ppm) |
[Ref.: #65226] |
Enrichment culture duration |
4 weeks |
[Ref.: #65226] |
Enrichment culture temperature |
28 ̊C |
[Ref.: #65226] |
Isolation/enrichment procedure |
incubated at 28°C and 150 r.p.m. in the dark for four weeks; and a 40 ml aliquot of the enriched culture was transferred into fresh benzo[a]pyrene -MM2 medium for the second enrichment. This step was repeated eight times, then serial dilutions of 10^-2, 10^-3, 10^-4 were selected and a 100 µl portion was spread on MM2 agar (1000 ml MM2 medium with 12 g agar) plate with additional benzo[a]pyrene [10 µl benzo[a]pyrene (10ppm) was spread on every plate]. The plates were aerobically incubated in the |
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[Ref.: #67770] |
Sample type/isolated from |
Mangrove sediment in Yunxiao Mangrove National Nature Reserve |
[Ref.: #67770] |
Country |
China |
[Ref.: #67770] |
Country ISO 3 Code |
CHN |
[Ref.: #67770] |
Continent |
Asia |
* marker position based on {}
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Isolation sources categories |
#Environmental |
#Aquatic |
#Mangrove |
#Environmental |
#Terrestrial |
#Sediment |
#Host |
#Plants |
#Tree |
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Availability in culture collections External links
[Ref.: #65226] |
Culture collection no. |
MCCC 1K03312, JCM 32104 |
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Literature: |
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Topic |
Title |
Authors |
Journal |
DOI |
Year |
|
Phylogeny |
Mangrovitalea sediminis gen. nov., sp. nov., a member of the family Alteromonadaceae isolated from mangrove sediment. |
Liao H, Li Y, Guo X, Lin X, Lai Q, Xu H, Zheng T, Tian Y |
Int J Syst Evol Microbiol |
10.1099/ijsem.0.002433 |
2017 |
* |
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- References
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#65226 |
Hu Liao, Yuqian Li, Xintong Guo, Xiaolan Lin, Qiliang Lai, Hong Xu, Tianling Zheng, Yun Tian:
Mangrovitalea sediminis gen. nov., sp. nov., a member of the family Alteromonadaceae isolated from mangrove sediment.
IJSEM 67:
5172 - 5178
2017 (
DOI 10.1099/ijsem.0.002433 , PubMed 29043950 )
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#67770 |
Japan Collection of Microorganism (JCM) ; Curators of the JCM;
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- * These data were automatically processed and therefore are not curated
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