Strain identifier

BacDive ID: 158495

Type strain: Yes

Species: Citroniella saccharovorans

Strain Designation: M6.X9

Strain history: <- P. Lawson, Dept. Botany and Microbiol., Univ. Oklahoma, USA; M6.X9 <- N. Patel

NCBI tax ID(s): 2053367 (species)

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General

@ref: 65171

BacDive-ID: 158495

DSM-Number: 29873

keywords: 16S sequence, Bacteria, anaerobe, mesophilic, Gram-positive, coccus-shaped, colony-forming

description: Citroniella saccharovorans M6.X9 is an anaerobe, mesophilic, Gram-positive bacterium that forms circular colonies and was isolated from human fecal sample.

NCBI tax id

  • NCBI tax id: 2053367
  • Matching level: species

strain history

  • @ref: 65171
  • history: <- P. Lawson, Dept. Botany and Microbiol., Univ. Oklahoma, USA; M6.X9 <- N. Patel

doi: 10.13145/bacdive158495.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Eubacteriales
  • family: Peptoniphilaceae
  • genus: Citroniella
  • species: Citroniella saccharovorans
  • full scientific name: Citroniella saccharovorans Patel et al. 2019

@ref: 65171

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Eubacteriales

family: Peptoniphilaceae

genus: Citroniella

species: Citroniella saccharovorans

full scientific name: Citroniella saccharovorans Patel et al. 2019

strain designation: M6.X9

type strain: yes

Morphology

cell morphology

  • @ref: 67842
  • gram stain: positive
  • cell shape: coccus-shaped
  • motility: no

colony morphology

  • @ref: 67842
  • colony size: 1 mm
  • colony color: white
  • colony shape: circular
  • incubation period: 6 days
  • medium used: Brucella blood agar

Culture and growth conditions

culture medium

  • @ref: 65171
  • name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/693
  • composition: Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base

culture temp

@refgrowthtypetemperaturerange
67842positivegrowth25-37mesophilic
67842positiveoptimum30mesophilic

culture pH

@refabilitytypepHPH range
67842positivegrowth6.5-9alkaliphile
67842positiveoptimum6.5-7.3

Physiology and metabolism

oxygen tolerance

  • @ref: 67842
  • oxygen tolerance: anaerobe

spore formation

  • @ref: 67842
  • spore formation: no

halophily

@refsaltgrowthtested relationconcentration
67842NaClpositivegrowth0.5-6 %(w/v)
67842NaClpositiveoptimum1-3 %(w/v)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6784217665alpha-D-glucose 6-phosphate+assimilation
6784217057cellobiose+assimilation
6784223652dextrin+assimilation
67842739183-O-methyl-D-glucose+assimilation
6784217306maltose+assimilation
6784261993maltotriose+assimilation
6784218394palatinose+assimilation
6784236655glyoxylate+assimilation
6784232528turanose+assimilation
6784215361pyruvate+assimilation
6784251850methyl pyruvate+assimilation
6784217196L-asparagine+assimilation
6784218050L-glutamine+assimilation
6784216643L-methionine+assimilation
6784217203L-proline+assimilation
6784217115L-serine+assimilation
6784216857L-threonine+assimilation
6784217596inosine+assimilation
6784216704uridine+assimilation
6784259640N-acetylglucosamine+assimilation
6784215824D-fructose+assimilation
6784218287L-fucose+assimilation
6784212936D-galactose+assimilation
678426359lactulose+assimilation
6784228034beta-D-galactoside+assimilation
6784217748thymidine+assimilation
6784217234glucose+fermentation
6784217306maltose+fermentation

metabolite production

  • @ref: 67842
  • Chebi-ID: 35581
  • metabolite: indole
  • production: yes

enzymes

@refvalueactivityec
67842catalase-1.11.1.6
67842urease-3.5.1.5
67842arginine arylamidase+
67842leucine arylamidase+3.4.11.1
67842tyrosine arylamidase+

fatty acid profile

  • fatty acids

    @reffatty acidpercentageECL
    67842C10:04.6
    67842C12:02
    67842C14:04.3
    67842C15:06.9
    67842C16:021.7
    67842C17:1 w8c15
    67842C17:1 w6c4
    67842C17:07.6
    67842C17:0 Cyclo / C18:07
    67842C18:1 w7c4.4
    67842C18:1 w9c12
    67842C18:2 w6,9c / C18:0 anteiso6.3
    67842Unidentified ECL C17.0451.417.045
    67842Unidentified ECL C18.4652.918.465
  • type of FA analysis: whole cell analysis
  • incubation medium: Brucella agar with 5 % sheep blood
  • agar/liquid: agar
  • incubation temperature: 37
  • incubation time: 6
  • software version: Sherlock 6.1
  • library/peak naming table: QBA1
  • system: MIS MIDI
  • cutoff value:

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentenrichment cultureenrichment culture compositionenrichment culture durationenrichment culture temperatureisolation procedure
65171human fecal sampleUniversidad Cientifica del SurPeruPERMiddle and South America
67842freshly voided human fecal sample obtained from one member of a traditional coastal Peruvian communitySouthern coastPeruPERMiddle and South Americamodified Medium 2supplemented with xylan (per 100 ml distilled water): Casitone (1.0 g), yeast extract (0.25 g), minerals solution (A) (15.0 ml), minerals solution (B) (15.0 ml), clarified rumen fluid (20 ml), resazurin (0.0001 g), sodium lactate (70 % w/v) (1.0 g), xylan (0.2 g), cysteine HCl (0.05 g), sodium bicarbonate (0.4 g), distilled water (to 1000 ml). Minerals solution (A) contained (per 1000 ml); K2HPO4 (3.0 g). Minerals solution (B) contained (per 1000 ml); KH2PO4 (3.0 g), (NH4)2SO4 (6.0 g), NaCl (6.0 g), MgSO4 x 7H2O (0.6 g), CaCl2 (0.6 g)14 days371.0 ml fecal slurry in a sealed serum bottle containing 100 ml modified Medium 2. The enrichment was incubated at 37°C for 14 days with a gas mix of 5 % hydrogen, 10 % carbon dioxide and 85 % nitrogen to maintain anoxic conditions. Throughout the 14-day period, a sample of the enrichment was transferred onto Medium 2 agar and after a 5-day incubation, colonies were picked and sub-cultured onto the same medium. All isolates were sub-cultured on Bacto Brain Heart Infusion supplemented with 5 % defibrinated sheep's blood agar plates (pH 7.4).

isolation source categories

Cat1Cat2Cat3
#Host Body Product#Gastrointestinal tract#Feces (Stool)
#Host#Human

taxonmaps

  • @ref: 69479
  • File name: preview.99_166992.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_7499;96_62489;97_80989;98_109623;99_166992&stattab=map
  • Last taxonomy: Peptoniphilaceae
  • 16S sequence: MG496017
  • Sequence Identity:
  • Total samples: 1630
  • soil counts: 35
  • aquatic counts: 40
  • animal counts: 1547
  • plant counts: 8

Sequence information

16S sequences

  • @ref: 65171
  • description: Peptoniphilaceae bacterium M6.X9 16S ribosomal RNA gene, partial sequence
  • accession: MG496017
  • length: 1413
  • database: ena
  • NCBI tax ID: 2053367

GC content

  • @ref: 67842
  • GC-content: 29.9
  • method: genome sequence analysis

External links

@ref: 65171

culture collection no.: DSM 29873, CCUG 66799

straininfo link

  • @ref: 111290
  • straininfo: 399009

literature

  • topic: Phylogeny
  • Pubmed-ID: 30767850
  • title: Citroniella saccharovorans gen. nov. sp. nov., a member of the family Peptoniphilaceae isolated from a human fecal sample from a coastal traditional community member.
  • authors: Patel NB, Obregon-Tito AJ, Tito RY, Trujillo-Villaroel O, Marin-Reyes L, Troncoso-Corzo L, Guija-Poma E, Lewis CM Jr, Lawson PA
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.003287
  • year: 2019
  • mesh: Bacterial Typing Techniques, Base Composition, Clostridiales/*classification/isolation & purification, DNA, Bacterial/genetics, Fatty Acids/chemistry, Feces/*microbiology, Humans, Peru, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA
  • topic2: Pathogenicity

Reference

@idauthorstitledoi/urlcataloguejournal
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
65171Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-29873Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 29873)
67842Nisha B. Patel, Alexandra J. Obregón-Tito, Raul Y. Tito, Omar Trujillo-Villaroel, Luis Marin-Reyes, Luzmila Troncoso-Corzo, Emilio Guija-Poma, Cecil M. Lewis Jr, Paul A. LawsonCitroniella saccharovorans gen. nov. sp. nov., a member of the family Peptoniphilaceae isolated from a human fecal sample from a coastal traditional community member10.1099/ijsem.0.003287IJSEM 69: 1142-1148 2019
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
111290Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID399009.1