Strain identifier

BacDive ID: 158495

Type strain: Yes

Species: Citroniella saccharovorans

Strain Designation: M6.X9

Culture col. no.: DSM 29873, CCUG 66799

Strain history: <- P. Lawson, Dept. Botany and Microbiol., Univ. Oklahoma, USA; M6.X9 <- N. Patel

NCBI tax ID(s): 2053367 (species)

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Citroniella saccharovorans M6.X9 is an anaerobe, mesophilic, Gram-positive bacterium that forms circular colonies and was isolated from human fecal sample.

  1. coccus-shaped
  2. colony-forming
  3. Gram-positive
  4. anaerobe
  5. mesophilic
  6. 16S sequence
  7. Bacteria
  • Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental informationarrow_down
  • [Ref.: #65171] Sample type/isolated from human fecal sample
    [Ref.: #65171] Geographic location (country and/or sea, region) Universidad Cientifica del Sur
    [Ref.: #65171] Country Peru
    [Ref.: #65171] Country ISO 3 Code PER
    [Ref.: #65171] Continent Middle and South America
     
    [Ref.: #67842] Sample type/isolated from freshly voided human fecal sample obtained from one member of a traditional coastal Peruvian community
    [Ref.: #67842] Geographic location (country and/or sea, region) Southern coast
    [Ref.: #67842] Country Peru
    [Ref.: #67842] Country ISO 3 Code PER
    [Ref.: #67842] Continent Middle and South America
    [Ref.: #67842] Enrichment culture modified Medium 2
    [Ref.: #67842] Enrichment culture composition supplemented with xylan (per 100 ml distilled water): Casitone (1.0 g), yeast extract (0.25 g), minerals solution (A) (15.0 ml), minerals solution (B) (15.0 ml), clarified rumen fluid (20 ml), resazurin (0.0001 g), sodium lactate (70 % w/v) (1.0 g), xylan (0.2 g), cysteine HCl (0.05 g), sodium bicarbonate (0.4 g), distilled water (to 1000 ml). Minerals solution (A) contained (per 1000 ml); K2HPO4 (3.0 g). Minerals solution (B) contained (per 1000 ml); KH2PO4 (3.0 g), (NH4)2SO4 (6.0 g), NaCl (6.0 g), MgSO4 x 7H2O (0.6 g), CaCl2 (0.6 g)
    [Ref.: #67842] Enrichment culture duration 14 days
    [Ref.: #67842] Enrichment culture temperature 37  ̊C
    [Ref.: #67842] Isolation/enrichment procedure 1.0 ml fecal slurry in a sealed serum bottle containing 100 ml modified Medium 2. The enrichment was incubated at 37°C for 14 days with a gas mix of 5 % hydrogen, 10 % carbon dioxide and 85 % nitrogen to maintain anoxic conditions. Throughout the 14-day period, a sample of the enrichment was transferred onto Medium 2 agar and after a 5-day incubation, colonies were picked and sub-cultured onto the same medium. All isolates were sub-cultured on Bacto Brain Heart Infusion supplemented with 5 % defibrinated sheep's blood agar plates (pH 7.4).
    * marker position based on {}
    Isolation sources categories
    #Host Body Product #Gastrointestinal tract #Feces (Stool)
    #Host #Human    -   

    Global distribution of 16S sequence MG496017 (>99% sequence identity) for Peptoniphilaceae from Microbeatlas

    Aquatic Samples 40
    Soil Samples 35
    Animal Samples 1547
    Plant Samples 8
    Total Samples 1630

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