[Ref.: #65171] |
Sample type/isolated from |
human fecal sample |
[Ref.: #65171] |
Geographic location (country and/or sea, region) |
Universidad Cientifica del Sur |
[Ref.: #65171] |
Country |
Peru |
[Ref.: #65171] |
Country ISO 3 Code |
PER |
[Ref.: #65171] |
Continent |
Middle and South America |
|
[Ref.: #67842] |
Sample type/isolated from |
freshly voided human fecal sample obtained from one member of a traditional coastal Peruvian community |
[Ref.: #67842] |
Geographic location (country and/or sea, region) |
Southern coast |
[Ref.: #67842] |
Country |
Peru |
[Ref.: #67842] |
Country ISO 3 Code |
PER |
[Ref.: #67842] |
Continent |
Middle and South America |
[Ref.: #67842] |
Enrichment culture |
modified Medium 2 |
[Ref.: #67842] |
Enrichment culture composition |
supplemented with xylan (per 100 ml distilled water): Casitone (1.0 g), yeast extract (0.25 g), minerals solution (A) (15.0 ml), minerals solution (B) (15.0 ml), clarified rumen fluid (20 ml), resazurin (0.0001 g), sodium lactate (70 % w/v) (1.0 g), xylan (0.2 g), cysteine HCl (0.05 g), sodium bicarbonate (0.4 g), distilled water (to 1000 ml). Minerals solution (A) contained (per 1000 ml); K2HPO4 (3.0 g). Minerals solution (B) contained (per 1000 ml); KH2PO4 (3.0 g), (NH4)2SO4 (6.0 g), NaCl (6.0 g), MgSO4 x 7H2O (0.6 g), CaCl2 (0.6 g) |
[Ref.: #67842] |
Enrichment culture duration |
14 days |
[Ref.: #67842] |
Enrichment culture temperature |
37 ̊C |
[Ref.: #67842] |
Isolation/enrichment procedure |
1.0 ml fecal slurry in a sealed serum bottle containing 100 ml modified Medium 2. The enrichment was incubated at 37°C for 14 days with a gas mix of 5 % hydrogen, 10 % carbon dioxide and 85 % nitrogen to maintain anoxic conditions. Throughout the 14-day period, a sample of the enrichment was transferred onto Medium 2 agar and after a 5-day incubation, colonies were picked and sub-cultured onto the same medium. All isolates were sub-cultured on Bacto Brain Heart Infusion supplemented with 5 % defibrinated sheep's blood agar plates (pH 7.4). |
* marker position based on {}
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Isolation sources categories |
#Host Body Product |
#Gastrointestinal tract |
#Feces (Stool) |
#Host |
#Human |
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Availability in culture collections External links
[Ref.: #65171] |
Culture collection no. |
DSM 29873, CCUG 66799 |
[Ref.: #111290] |
SI-ID 399009
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Literature: |
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Topic |
Title |
Authors |
Journal |
DOI |
Year |
|
Phylogeny |
Citroniella saccharovorans gen. nov. sp. nov., a member of the family Peptoniphilaceae isolated from a human fecal sample from a coastal traditional community member. |
Patel NB, Obregon-Tito AJ, Tito RY, Trujillo-Villaroel O, Marin-Reyes L, Troncoso-Corzo L, Guija-Poma E, Lewis CM Jr, Lawson PA |
Int J Syst Evol Microbiol |
10.1099/ijsem.0.003287 |
2019 |
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- References
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#65171 |
Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ;
DSM 29873
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#69479 |
João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.:
MicrobeAtlas 1.0 beta
.
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#111290 |
Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.:
StrainInfo: A central database for resolving microbial strain identifiers
.
(
DOI 10.60712/SI-ID399009.1 )
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- * These data were automatically processed and therefore are not curated
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