Strain identifier

BacDive ID: 158440

Type strain: Yes

Species: Sutterella megalosphaeroides

Strain Designation: 6FBBBH3

Strain history: M. Sakamoto; RIKEN BRC-JCM, Japan; 6FBBBH3.

NCBI tax ID(s): 2494234 (species)

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General

@ref: 65116

BacDive-ID: 158440

DSM-Number: 106861

keywords: genome sequence, 16S sequence, Bacteria, obligate anaerobe, mesophilic, Gram-negative, coccus-shaped, colony-forming

description: Sutterella megalosphaeroides 6FBBBH3 is an obligate anaerobe, mesophilic, Gram-negative bacterium that forms circular colonies and was isolated from Feces of man.

NCBI tax id

  • NCBI tax id: 2494234
  • Matching level: species

strain history

@refhistory
65116<- J. M. Sakamoto, RIKEN, Wako, Saitama, Japan; 6FBBBH3
67770M. Sakamoto; RIKEN BRC-JCM, Japan; 6FBBBH3.

doi: 10.13145/bacdive158440.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Betaproteobacteria
  • order: Burkholderiales
  • family: Sutterellaceae
  • genus: Sutterella
  • species: Sutterella megalosphaeroides
  • full scientific name: Sutterella megalosphaeroides Sakamoto et al. 2018

@ref: 65116

domain: Bacteria

phylum: Proteobacteria

class: Betaproteobacteria

order: Burkholderiales

family: Sutterellaceae

genus: Sutterella

species: Sutterella megalosphaeroides

full scientific name: Sutterella megalosphaeroides Sakamoto et al. 2018

strain designation: 6FBBBH3

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell shapemotilityconfidence
66790negative1.7-2.1 µmcoccus-shapedno
69480negative99.999

colony morphology

  • @ref: 66790
  • colony size: 0.5-1 mm
  • colony shape: circular
  • incubation period: 4 days
  • medium used: Brucella blood agar

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
65116COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
66790Brucella blood agaryes

culture temp

@refgrowthtypetemperaturerange
65116positivegrowth37mesophilic
66790positiveoptimum37mesophilic
66790positivegrowth20-45
67770positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 66790
  • oxygen tolerance: obligate anaerobe

spore formation

@refspore formationconfidence
66790no
69480no99.995
69481no100

observation

@refobservation
66790Cells occurred in singly.
66790Growth occurs weakly on medium containing 5 or 20 % (w/v) bile.
67770quinones: MK-5, MMK-5

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
667905291gelatin-degradation
6679016634raffinose-fermentation
6679037684mannose-fermentation
667904853esculin-hydrolysis
6679017632nitrate-reduction

antibiotic resistance

@refChEBImetaboliteis antibioticis resistantresistance conc.is sensitivesensitivity conc.group ID
667902676amoxicillinyesyes25 µg (disc)
6679017698chloramphenicolyesyes30 µg (disc)
66790100241ciprofloxacinyesyes5 µg (disc)
6679048923erythromycinyesyes15 µg (disc)
6679017833gentamicinyesyes10 µg (disc)
667906104kanamycinyesyes30 µg (disc)
667907507neomycinyesyes30 µg (disc)
6679027701oxytetracyclineyesyes30 µg (disc)
6679017076streptomycinyesyes10 µg (disc)
6679017334penicillinyesyes10 Unit (disc)
6679028669bacitracinyesyes10 Unit (disc)
6679037943colistinyesyes10 µg (disc)
6679028001vancomycinyesyes30 µg (disc)
6679045924trimethoprimyesyes1.25 µg (disc)26
667909332sulfamethoxazoleyesyes23.75 µg (disc)26

metabolite production

  • @ref: 66790
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
667906-phospho-beta-galactosidase-3.2.1.85
66790acid phosphatase-3.1.3.2
66790alpha-arabinosidase-3.2.1.55
66790alpha-fucosidase-3.2.1.51
66790alpha-galactosidase-3.2.1.22
66790alpha-glucosidase-3.2.1.20
66790alpha-mannosidase-3.2.1.24
66790arginine dihydrolase-3.5.3.6
66790beta-galactosidase-3.2.1.23
66790beta-glucosidase-3.2.1.21
66790beta-glucuronidase-3.2.1.31
66790catalase-1.11.1.6
66790chymotrypsin-3.4.4.5
66790cystine arylamidase-3.4.11.3
66790esterase Lipase (C 8)-
66790glutamate decarboxylase-4.1.1.15
66790glutamyl-glutamate arylamidase-
66790glycin arylamidase-
66790histidine arylamidase-
66790leucyl glycin arylamidase-3.4.11.1
66790lipase-
66790N-acetyl-beta-glucosaminidase-3.2.1.52
66790phenylalanine arylamidase-
66790proline-arylamidase-3.4.11.5
66790pyroglutamic acid arylamidase-
66790serine arylamidase-
66790trypsin-3.4.21.4
66790tyrosine arylamidase-
66790urease-3.5.1.5
66790valine arylamidase-
66790alanine arylamidase+3.4.11.2
66790alkaline phosphatase+3.1.3.1
66790arginine arylamidase+
66790esterase+
66790leucine arylamidase+3.4.11.1
66790naphthol-AS-BI-phosphohydrolase+

fatty acid profile

  • fatty acids

    @reffatty acidpercentageSD
    66790C18:2 w6,9c12.51.7
    66790C15:0 DMA and/or C14:0 3OH2.4
    66790C18:1 w9c29.57.1
    66790C14:05.11.8
    66790C16:033.51.7
    66790C18:07.11.8
    66790C16:1 w7c13.254.7
  • type of FA analysis: whole cell analysis
  • incubation medium: Brucella blood agar
  • agar/liquid: agar
  • incubation temperature: 37
  • incubation time: 4
  • software version: Sherlock 6.2B
  • library/peak naming table: BHIBLA 3.80
  • system: MIS MIDI
  • method/protocol: Miller 1982

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentsampling dateenrichment cultureenrichment culture compositionenrichment culture durationenrichment culture temperatureisolation procedure
65116Feces of manTsukubaJapanJPNAsia
66790faecal sample of a healthy Japanese man (37 years old)Tsukuba, IbarakiJapanJPNAsia2017-02Brucella blood agarwith haemin and menadione2-4 days37Each dilution of the fresh faecal samples was plated onto Brucella blood agar and incubated for 2-4 days at 37°C under a H2/CO2/N2 (1:1:8, by vol.) gas mixture.
67770Feces of a man in the thirtiesJapanJPNAsia

isolation source categories

Cat1Cat2Cat3
#Host Body Product#Gastrointestinal tract#Feces (Stool)
#Host#Human#Male

Sequence information

16S sequences

  • @ref: 66790
  • description: Sutterella megalosphaeroides 6FBBBH3 gene for 16S ribosomal RNA, partial sequence
  • accession: LC388666
  • length: 1472
  • database: ena
  • NCBI tax ID: 2494234

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Sutterella megalosphaeroides 6FBBBH3GCA_003609995completencbi2494234
66792Sutterella sp. 6FBBBH32259134.3completepatric2494234
66792Sutterella megalosphaeroides 6FBBBH32833028006completeimg2494234

GC content

  • @ref: 66790
  • GC-content: 62.8
  • method: genome sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileno92.091no
flagellatedno95.855yes
gram-positiveno98.118yes
anaerobicyes98.414yes
aerobicno96.61yes
halophileno78.762no
spore-formingno97.6yes
glucose-utilno69.74no
thermophileno99.408yes
glucose-fermentno77.193no

External links

@ref: 65116

culture collection no.: DSM 106861, JCM 32470

straininfo link

  • @ref: 111241
  • straininfo: 408099

literature

  • topic: Phylogeny
  • Pubmed-ID: 30394865
  • title: Mesosutterella multiformis gen. nov., sp. nov., a member of the family Sutterellaceae and Sutterella megalosphaeroides sp. nov., isolated from human faeces.
  • authors: Sakamoto M, Ikeyama N, Kunihiro T, Iino T, Yuki M, Ohkuma M
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.003096
  • year: 2018
  • mesh: Alcaligenaceae/classification, Bacterial Typing Techniques, Base Composition, Burkholderiales/*classification/genetics/isolation & purification, DNA, Bacterial/genetics, Fatty Acids/chemistry, Feces/*microbiology, Female, Humans, Japan, Male, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistry
  • topic2: Pathogenicity

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
65116Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-106861Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 106861)
66790Mitsuo Sakamoto, Nao Ikeyama, Tadao Kunihiro, Takao Iino, Masahiro Yuki, Moriya OhkumaMesosutterella multiformis gen. nov., sp. nov., a member of the family Sutterellaceae and Sutterella megalosphaeroides sp. nov., isolated from human faeces10.1099/ijsem.0.003096IJSEM 68: 3942-3950 201830394865
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar SchomburgBRENDA, the ELIXIR core data resource in 2021: new developments and updates10.1093/nar/gkaa1025Nucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1
111241Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID408099.1