Sutterella megalosphaeroides (DSM 106861, JCM 32470, 6FBBBH3)

Name: Sutterella megalosphaeroides (DSM 106861, JCM 32470, 6FBBBH3)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 158440

Type strain

Strain Designation 6FBBBH3

Culture col. no. DSM 106861 JCM 32470 6FBBBH3

NCBI tax ID(s) 2494234

Special Collections DSMZ JCM Literature strains

History DSMZ <- J. M. Sakamoto, RIKEN, Wako, Saitama, Japan; 6FBBBH3 JCM <-- M. Sakamoto; RIKEN BRC-JCM, Japan; 6FBBBH3.

Links

Sutterella megalosphaeroides 6FBBBH3 is an obligate anaerobe, Gram-negative, coccus-shaped bacterium that forms circular colonies and was isolated from Feces of man.

Gram-negative coccus-shaped colony-forming obligate anaerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 408099

JCM 32470,DSM 106861

@ref 20215

Last LPSN update 2026-05-29 08:43:58

Domain Bacteria

Phylum Pseudomonadota

Class Betaproteobacteria

Order Burkholderiales

Family Sutterellaceae

Genus Sutterella

Species Sutterella megalosphaeroides

Full scientific name Sutterella megalosphaeroides Sakamoto et al. 2018

BacDive ID	Other strains from **Sutterella megalosphaeroides** (3)	Type strain
164266	S. megalosphaeroides JCM 32468
164267	S. megalosphaeroides JCM 32469
164268	S. megalosphaeroides JCM 32471

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell shape	Motility	Confidence
66790	negative	1.7-2.1 µm	coccus-shaped
125438	negative				97.744

Colony morphology

@ref	Colony size	Colony shape	Incubation period	Medium used
66790	0.5-1 mm	circular	4 days	Brucella blood agar

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
65116	COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)		Medium recipe at MediaDive	Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
66790	Brucella blood agar

Culture temp

@ref	Growth	Type	Temperature (°C)
65116	positive	growth	37
66790	positive	optimum	37
66790	positive	growth	20-45
67770	positive	growth	37

Physiology and metabolism

Oxygen tolerance

66790

Oxygen toleranceobligate anaerobe

Spore formation

@ref	Spore formation	Confidence
66790
125438		96.944
125439		94.82

Observation

@ref	Observation
66790	Cells occurred in singly.
66790	Growth occurs weakly on medium containing 5 or 20 % (w/v) bile.
67770	quinones: MK-5, MMK-5

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
66790	4853 ChEBI	esculin	-	hydrolysis
66790	5291 ChEBI	gelatin	-	degradation
66790	37684 ChEBI	mannose	-	fermentation
66790	17632 ChEBI	nitrate	-	reduction
66790	16634 ChEBI	raffinose	-	fermentation

Antibiotic resistance

@ref	ChEBI	Group ID	Metabolite	Resistance conc.	Sensitivity conc.
66790	2676		amoxicillin	25 µg (disc)
66790	28669		bacitracin		10 Unit (disc)
66790	17698		chloramphenicol		30 µg (disc)
66790	100241		ciprofloxacin		5 µg (disc)
66790	37943		colistin		10 µg (disc)
66790	48923		erythromycin		15 µg (disc)
66790	17833		gentamicin		10 µg (disc)
66790	6104		kanamycin		30 µg (disc)
66790	7507		neomycin		30 µg (disc)
66790	27701		oxytetracycline		30 µg (disc)
66790	17334		penicillin	10 Unit (disc)
66790	17076		streptomycin	10 µg (disc)
66790	9332	26	sulfamethoxazole	23.75 µg (disc)
66790	45924	26	trimethoprim	1.25 µg (disc)
66790	28001		vancomycin	30 µg (disc)

Metabolite production

@ref	Chebi-ID	Metabolite	Production
66790	35581 ChEBI	indole

Enzymes

@ref	Value	Activity	Ec
66790	6-phospho-beta-galactosidase	-	3.2.1.85
66790	acid phosphatase	-	3.1.3.2
66790	alanine arylamidase	+	3.4.11.2
66790	alkaline phosphatase	+	3.1.3.1
66790	alpha-arabinosidase	-	3.2.1.55
66790	alpha-fucosidase	-	3.2.1.51
66790	alpha-galactosidase	-	3.2.1.22
66790	alpha-glucosidase	-	3.2.1.20
66790	alpha-mannosidase	-	3.2.1.24
66790	arginine arylamidase	+
66790	arginine dihydrolase	-	3.5.3.6
66790	beta-galactosidase	-	3.2.1.23
66790	beta-glucosidase	-	3.2.1.21
66790	beta-glucuronidase	-	3.2.1.31
66790	catalase	-	1.11.1.6
66790	chymotrypsin	-	3.4.4.5
66790	cystine arylamidase	-	3.4.11.3
66790	esterase	+
66790	esterase Lipase (C 8)	-
66790	glutamate decarboxylase	-	4.1.1.15
66790	glutamyl-glutamate arylamidase	-
66790	glycin arylamidase	-
66790	histidine arylamidase	-
66790	leucine arylamidase	+	3.4.11.1
66790	leucyl glycin arylamidase	-	3.4.11.1
66790	lipase	-
66790	N-acetyl-beta-glucosaminidase	-	3.2.1.52
66790	naphthol-AS-BI-phosphohydrolase	+
66790	phenylalanine arylamidase	-
66790	proline-arylamidase	-	3.4.11.5
66790	pyroglutamic acid arylamidase	-
66790	serine arylamidase	-
66790	trypsin	-	3.4.21.4
66790	tyrosine arylamidase	-
66790	urease	-	3.5.1.5
66790	valine arylamidase	-

Pathway annotation

Computationally determined by

@ref	pathway	enzyme coverage	annotated reactions
66794	sulfopterin metabolism	100	4 of 4
66794	cardiolipin biosynthesis	100	7 of 7
66794	coenzyme A metabolism	100	4 of 4
66794	ppGpp biosynthesis	100	4 of 4
66794	CDP-diacylglycerol biosynthesis	100	2 of 2
66794	hydrogen production	100	5 of 5
66794	UDP-GlcNAc biosynthesis	100	3 of 3
66794	folate polyglutamylation	100	1 of 1
66794	suberin monomers biosynthesis	100	2 of 2
66794	anapleurotic synthesis of oxalacetate	100	1 of 1
66794	biotin biosynthesis	100	4 of 4
66794	palmitate biosynthesis	95.45	21 of 22
66794	vitamin B1 metabolism	92.31	12 of 13
66794	lipid A biosynthesis	88.89	8 of 9
66794	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	87.5	7 of 8
66794	purine metabolism	80.85	76 of 94
66794	methylglyoxal degradation	80	4 of 5
66794	peptidoglycan biosynthesis	80	12 of 15
66794	pyrimidine metabolism	80	36 of 45
66794	photosynthesis	78.57	11 of 14
66794	molybdenum cofactor biosynthesis	77.78	7 of 9
66794	phenylalanine metabolism	76.92	10 of 13
66794	CMP-KDO biosynthesis	75	3 of 4
66794	acetate fermentation	75	3 of 4
66794	proline metabolism	72.73	8 of 11
66794	NAD metabolism	72.22	13 of 18
66794	reductive acetyl coenzyme A pathway	71.43	5 of 7
66794	tetrahydrofolate metabolism	71.43	10 of 14
66794	acetoin degradation	66.67	2 of 3
66794	serine metabolism	66.67	6 of 9
66794	aspartate and asparagine metabolism	66.67	6 of 9
66794	octane oxidation	66.67	2 of 3
66794	L-lactaldehyde degradation	66.67	2 of 3
66794	methane metabolism	66.67	2 of 3
66794	ketogluconate metabolism	62.5	5 of 8
66794	flavin biosynthesis	60	9 of 15
66794	glycolysis	58.82	10 of 17
66794	oxidative phosphorylation	58.24	53 of 91
66794	isoprenoid biosynthesis	57.69	15 of 26
66794	ubiquinone biosynthesis	57.14	4 of 7
66794	propanol degradation	57.14	4 of 7
66794	heme metabolism	57.14	8 of 14
66794	CO2 fixation in Crenarchaeota	55.56	5 of 9
66794	d-mannose degradation	55.56	5 of 9
66794	valine metabolism	55.56	5 of 9
66794	non-pathway related	55.26	21 of 38
66794	glutamate and glutamine metabolism	53.57	15 of 28
66794	alanine metabolism	51.72	15 of 29
66794	tyrosine metabolism	50	7 of 14
66794	propionate fermentation	50	5 of 10
66794	ethanol fermentation	50	1 of 2
66794	C4 and CAM-carbon fixation	50	4 of 8
66794	cis-vaccenate biosynthesis	50	1 of 2
66794	isoleucine metabolism	50	4 of 8
66794	dTDPLrhamnose biosynthesis	50	4 of 8
66794	citric acid cycle	50	7 of 14
66794	gluconeogenesis	50	4 of 8
66794	lactate fermentation	50	2 of 4
66794	quinate degradation	50	1 of 2
66794	methionine metabolism	46.15	12 of 26
66794	urea cycle	46.15	6 of 13
66794	leucine metabolism	46.15	6 of 13
66794	vitamin B6 metabolism	45.45	5 of 11
66794	lipid metabolism	45.16	14 of 31
66794	nitrate assimilation	44.44	4 of 9
66794	allantoin degradation	44.44	4 of 9
66794	chorismate metabolism	44.44	4 of 9
66794	glutathione metabolism	42.86	6 of 14
66794	degradation of aromatic, nitrogen containing compounds	41.67	5 of 12
66794	phenylacetate degradation (aerobic)	40	2 of 5
66794	vitamin K metabolism	40	2 of 5
66794	threonine metabolism	40	4 of 10
66794	degradation of hexoses	38.89	7 of 18
66794	histidine metabolism	37.93	11 of 29
66794	tryptophan metabolism	36.84	14 of 38
66794	pentose phosphate pathway	36.36	4 of 11
66794	degradation of pentoses	35.71	10 of 28
66794	lysine metabolism	33.33	14 of 42
66794	cyanate degradation	33.33	1 of 3
66794	formaldehyde oxidation	33.33	1 of 3
66794	glycolate and glyoxylate degradation	33.33	2 of 6
66794	arginine metabolism	33.33	8 of 24
66794	selenocysteine biosynthesis	33.33	2 of 6
66794	IAA biosynthesis	33.33	1 of 3
66794	degradation of sugar alcohols	31.25	5 of 16
66794	sulfate reduction	30.77	4 of 13
66794	glycine metabolism	30	3 of 10
66794	coenzyme M biosynthesis	30	3 of 10
66794	cysteine metabolism	27.78	5 of 18
66794	ascorbate metabolism	27.27	6 of 22
66794	metabolism of disaccharids	27.27	3 of 11
66794	3-phenylpropionate degradation	26.67	4 of 15
66794	polyamine pathway	26.09	6 of 23
66794	carnitine metabolism	25	2 of 8
66794	toluene degradation	25	1 of 4
66794	glycogen biosynthesis	25	1 of 4
66794	cyclohexanol degradation	25	1 of 4
66794	butanoate fermentation	25	1 of 4
66794	phenylpropanoid biosynthesis	23.08	3 of 13
66794	phosphatidylethanolamine bioynthesis	23.08	3 of 13
66794	4-hydroxymandelate degradation	22.22	2 of 9

Fatty acid profile

Metadata FA analysis

@ref

66790

Fatty acid	Percentage	SD
C16:0	33.5	1.7
C18:1 w9c	29.5	7.1
C16:1 w7c	13.25	4.7
C18:2 w6,9c	12.5	1.7
C18:0	7.1	1.8
C14:0	5.1	1.8
C15:0 DMA and/or C14:0 3OH	2.4

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host Body Product	#Gastrointestinal tract	#Feces (Stool)
#Host	#Human	#Male

Isolation

@ref	Sample type	Host species	Sampling date	Geographic location	Country	Country ISO 3 Code	Continent	Enrichment culture	Enrichment culture composition	Enrichment culture duration	Enrichment culture temperature	Isolation procedure
65116	Feces of man			Tsukuba	Japan	JPN	Asia
66790	faecal sample of a healthy Japanese man (37 years old)	Homo sapiens	2017-02	Tsukuba, Ibaraki	Japan	JPN	Asia	Brucella blood agar	with haemin and menadione	2-4 days	37	Each dilution of the fresh faecal samples was plated onto Brucella blood agar and incubated for 2-4 days at 37°C under a H2/CO2/N2 (1:1:8, by vol.) gas mixture.
67770	Feces of a man in the thirties	Homo sapiens			Japan	JPN	Asia

Interaction and safety

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM360999v1 assembly for Sutterella megalosphaeroides 6FBBBH3	complete	GCA_003609995	2259134.3	2833028006	2494234	83.92

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
66790	Sutterella megalosphaeroides 6FBBBH3 gene for 16S ribosomal RNA, partial sequence	LC388666	1472		2494234

GC content

@ref	GC-content (mol%)	Method
66790	62.8	genome sequence analysis

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM360999v1 assembly for Sutterella megalosphaeroides 6FBBBH3
BV-BRC: 2259134.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	gram_stain	BacteriaNetⓘ	negative	68.14	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate anaerobe	66.12	no
125439	motility	BacteriaNetⓘ	yes	52.29	no
125439	spore_formation	BacteriaNetⓘ	no	94.82	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Sutterella megalosphaeroides 6FBBBH3
NCBI: GCA_003609995.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	97.74	yes
125438	anaerobic	anaerobicⓘ	yes	78.24	yes
125438	aerobic	aerobicⓘ	no	86.50	no
125438	spore-forming	spore-formingⓘ	no	96.94	yes
125438	thermophilic	thermophileⓘ	no	93.37	no
125438	flagellated	motile2+ⓘ	no	89.11	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Mesosutterella multiformis gen. nov., sp. nov., a member of the family Sutterellaceae and Sutterella megalosphaeroides sp. nov., isolated from human faeces.	Sakamoto M, Ikeyama N, Kunihiro T, Iino T, Yuki M, Ohkuma M	Int J Syst Evol Microbiol	10.1099/ijsem.0.003096	2018

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#65116	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 106861
#66790	Mitsuo Sakamoto, Nao Ikeyama, Tadao Kunihiro, Takao Iino, Masahiro Yuki, Moriya Ohkuma: Mesosutterella multiformis gen. nov., sp. nov., a member of the family Sutterellaceae and Sutterella megalosphaeroides sp. nov., isolated from human faeces. IJSEM 68: 3942 - 3950 2018 ( DOI 10.1099/ijsem.0.003096 , PubMed 30394865 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#66794	Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Sutterella megalosphaeroides (DSM 106861, JCM 32470, 6FBBBH3)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Observation

Metabolite utilization

Antibiotic resistance

Metabolite production

Enzymes

Pathway annotation

Fatty acid profile

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Sequence information

Genome sequences

Genome browser: GCA_003609995

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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