Name: Sutterella megalosphaeroides 6FBBBH3
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 158440

Type strain:

Species: Sutterella megalosphaeroides

Strain Designation: 6FBBBH3

Culture col. no.: DSM 106861, JCM 32470

Strain history: M. Sakamoto; RIKEN BRC-JCM, Japan; 6FBBBH3.

NCBI tax ID(s): 2494234 (species)

Other strains from Sutterella megalosphaeroides

S. megalosphaeroides JCM 32468
S. megalosphaeroides JCM 32469
S. megalosphaeroides JCM 32471

Section

Sutterella megalosphaeroides 6FBBBH3 is an obligate anaerobe, mesophilic, Gram-negative bacterium that forms circular colonies and was isolated from Feces of man.

coccus-shaped
colony-forming
Gram-negative
mesophilic
obligate anaerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	07-12-2022 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Betaproteobacteria*
	Order	*Burkholderiales*
	Family	*Sutterellaceae*
	Genus	*Sutterella*
	Species	**Sutterella megalosphaeroides**
	Full Scientific Name (PNU)	Sutterella megalosphaeroides Sakamoto et al. 2018

Information on morphological and physiological properties Morphology

[Ref.: #66790]	Gram stain	negative
[Ref.: #66790]	Cell length	1.7-2.1 µm
[Ref.: #66790]	Cell shape	coccus-shaped
[Ref.: #66790]	Motility	no

[Ref.: #69480]	Gram stain	negative Confidence in %99.999

[Ref.: #66790]	Colony size	0.5-1 mm
[Ref.: #66790]	Colony shape	circular
[Ref.: #66790]	Incubation period	4 days
[Ref.: #66790]	Cultivation medium used	Brucella blood agar

Information on culture and growth conditions Culture and growth conditions

[Ref.: #65116]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #65116]

Culture medium growth

[Ref.: #65116]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #65116]

Culture medium composition

expand / minimize

[Ref.: #66790]

Culture medium

Brucella blood agar

[Ref.: #66790]

Culture medium growth

		Temperature
[Ref.: #65116]	growth	37 °C
[Ref.: #66790]	optimum	37 °C
[Ref.: #66790]	growth	20-45 °C
[Ref.: #67770]	growth	37 °C

[Ref.: #65116]	Temperature range	mesophilic
[Ref.: #66790]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #66790]

Oxygen tolerance

obligate anaerobe

[Ref.: #66790]	Ability of spore formation	no
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.995
[Ref.: #69481]	Ability of spore formation	no Confidence in %100

[Ref.: #66790]	Observation	Cells occurred in singly.
[Ref.: #66790]	Observation	Growth occurs weakly on medium containing 5 or 20 % (w/v) bile.
[Ref.: #67770]	Observation	quinones: MK-5, MMK-5

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #66790]	esculin ChEBI	-	hydrolysis
[Ref.: #66790]	gelatin ChEBI	-	degradation
[Ref.: #66790]	mannose ChEBI	-	fermentation
[Ref.: #66790]	nitrate ChEBI	-	reduction
[Ref.: #66790]	raffinose ChEBI	-	fermentation

	Metabolite	Antibiotic sensitivity	Concentration		Antibiotic resistance	Concentration
[Ref.: #66790]	amoxicillin ChEBI				yes	25	µg (disc)
[Ref.: #66790]	bacitracin ChEBI	yes	10	Unit (disc)
[Ref.: #66790]	chloramphenicol ChEBI	yes	30	µg (disc)
[Ref.: #66790]	ciprofloxacin ChEBI	yes	5	µg (disc)
[Ref.: #66790]	colistin ChEBI	yes	10	µg (disc)
[Ref.: #66790]	erythromycin ChEBI	yes	15	µg (disc)
[Ref.: #66790]	gentamicin ChEBI	yes	10	µg (disc)
[Ref.: #66790]	kanamycin ChEBI	yes	30	µg (disc)
[Ref.: #66790]	neomycin ChEBI	yes	30	µg (disc)
[Ref.: #66790]	oxytetracycline ChEBI	yes	30	µg (disc)
[Ref.: #66790]	penicillin ChEBI				yes	10	Unit (disc)
[Ref.: #66790]	streptomycin ChEBI				yes	10	µg (disc)
[Ref.: #66790]	vancomycin ChEBI				yes	30	µg (disc)
	Only first 10 entries are displayed. Click here to see all.

	Antibiotica	Group ID of combined antibiotics	Metabolite	Antibiotic resistance	Concentration
[Ref.: #66790]		26	sulfamethoxazole ChEBI	yes	23.75	µg (disc)
[Ref.: #66790]		26	trimethoprim ChEBI	yes	1.25	µg (disc)

	Metabolite production	Metabolite
[Ref.: #66790]		indole ChEBI

	Enzyme	Enzyme activity	EC number
[Ref.: #66790]	6-phospho-beta-galactosidase	-	3.2.1.85
[Ref.: #66790]	acid phosphatase	-	3.1.3.2
[Ref.: #66790]	alanine arylamidase	+	3.4.11.2
[Ref.: #66790]	alkaline phosphatase	+	3.1.3.1
[Ref.: #66790]	alpha-arabinosidase	-	3.2.1.55
[Ref.: #66790]	alpha-fucosidase	-	3.2.1.51
[Ref.: #66790]	alpha-galactosidase	-	3.2.1.22
[Ref.: #66790]	alpha-glucosidase	-	3.2.1.20
[Ref.: #66790]	alpha-mannosidase	-	3.2.1.24
[Ref.: #66790]	arginine arylamidase	+
[Ref.: #66790]	arginine dihydrolase	-	3.5.3.6
[Ref.: #66790]	beta-galactosidase	-	3.2.1.23
[Ref.: #66790]	beta-glucosidase	-	3.2.1.21
[Ref.: #66790]	beta-glucuronidase	-	3.2.1.31
[Ref.: #66790]	catalase	-	1.11.1.6
[Ref.: #66790]	chymotrypsin	-	3.4.4.5
[Ref.: #66790]	cystine arylamidase	-	3.4.11.3
[Ref.: #66790]	esterase	+
[Ref.: #66790]	esterase Lipase (C 8)	-
[Ref.: #66790]	glutamate decarboxylase	-	4.1.1.15
[Ref.: #66790]	glutamyl-glutamate arylamidase	-
[Ref.: #66790]	glycin arylamidase	-
[Ref.: #66790]	histidine arylamidase	-
[Ref.: #66790]	leucine arylamidase	+	3.4.11.1
[Ref.: #66790]	leucyl glycin arylamidase	-	3.4.11.1
[Ref.: #66790]	lipase	-
[Ref.: #66790]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #66790]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #66790]	phenylalanine arylamidase	-
[Ref.: #66790]	proline-arylamidase	-	3.4.11.5
[Ref.: #66790]	pyroglutamic acid arylamidase	-
[Ref.: #66790]	serine arylamidase	-
[Ref.: #66790]	trypsin	-	3.4.21.4
[Ref.: #66790]	tyrosine arylamidase	-
[Ref.: #66790]	urease	-	3.5.1.5
[Ref.: #66790]	valine arylamidase	-
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #66790]

Fatty acid	Percentage	SD
C16:0	33.5	1.7
C18:1 w9c	29.5	7.1
C16:1 w7c	13.25	4.7
C18:2 w6,9c	12.5	1.7
C18:0	7.1	1.8
C14:0	5.1	1.8
C15:0 DMA and/or C14:0 3OH	2.4

	Pathway	Enzyme coverage	Annotated reactions
	Pathway annotation	Computationally determined by
[Ref.: #66794]	sulfopterin metabolism	100	4 of 4
[Ref.: #66794]	cardiolipin biosynthesis	100	7 of 7
[Ref.: #66794]	coenzyme A metabolism	100	4 of 4
[Ref.: #66794]	ppGpp biosynthesis	100	4 of 4
[Ref.: #66794]	CDP-diacylglycerol biosynthesis	100	2 of 2
[Ref.: #66794]	hydrogen production	100	5 of 5
[Ref.: #66794]	UDP-GlcNAc biosynthesis	100	3 of 3
[Ref.: #66794]	folate polyglutamylation	100	1 of 1
[Ref.: #66794]	suberin monomers biosynthesis	100	2 of 2
[Ref.: #66794]	anapleurotic synthesis of oxalacetate	100	1 of 1
[Ref.: #66794]	biotin biosynthesis	100	4 of 4
[Ref.: #66794]	palmitate biosynthesis	95.45	21 of 22
[Ref.: #66794]	vitamin B1 metabolism	92.31	12 of 13
[Ref.: #66794]	lipid A biosynthesis	88.89	8 of 9
[Ref.: #66794]	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	87.5	7 of 8
[Ref.: #66794]	purine metabolism	80.85	76 of 94
[Ref.: #66794]	methylglyoxal degradation	80	4 of 5
[Ref.: #66794]	peptidoglycan biosynthesis	80	12 of 15
[Ref.: #66794]	pyrimidine metabolism	80	36 of 45
[Ref.: #66794]	photosynthesis	78.57	11 of 14
[Ref.: #66794]	molybdenum cofactor biosynthesis	77.78	7 of 9
[Ref.: #66794]	phenylalanine metabolism	76.92	10 of 13
[Ref.: #66794]	CMP-KDO biosynthesis	75	3 of 4
[Ref.: #66794]	acetate fermentation	75	3 of 4
[Ref.: #66794]	proline metabolism	72.73	8 of 11
[Ref.: #66794]	NAD metabolism	72.22	13 of 18
[Ref.: #66794]	reductive acetyl coenzyme A pathway	71.43	5 of 7
[Ref.: #66794]	tetrahydrofolate metabolism	71.43	10 of 14
[Ref.: #66794]	acetoin degradation	66.67	2 of 3
[Ref.: #66794]	serine metabolism	66.67	6 of 9
[Ref.: #66794]	aspartate and asparagine metabolism	66.67	6 of 9
[Ref.: #66794]	octane oxidation	66.67	2 of 3
[Ref.: #66794]	L-lactaldehyde degradation	66.67	2 of 3
[Ref.: #66794]	methane metabolism	66.67	2 of 3
[Ref.: #66794]	ketogluconate metabolism	62.5	5 of 8
[Ref.: #66794]	flavin biosynthesis	60	9 of 15
[Ref.: #66794]	glycolysis	58.82	10 of 17
[Ref.: #66794]	oxidative phosphorylation	58.24	53 of 91
[Ref.: #66794]	isoprenoid biosynthesis	57.69	15 of 26
[Ref.: #66794]	ubiquinone biosynthesis	57.14	4 of 7
[Ref.: #66794]	propanol degradation	57.14	4 of 7
[Ref.: #66794]	heme metabolism	57.14	8 of 14
[Ref.: #66794]	CO2 fixation in Crenarchaeota	55.56	5 of 9
[Ref.: #66794]	d-mannose degradation	55.56	5 of 9
[Ref.: #66794]	valine metabolism	55.56	5 of 9
[Ref.: #66794]	non-pathway related	55.26	21 of 38
[Ref.: #66794]	glutamate and glutamine metabolism	53.57	15 of 28
[Ref.: #66794]	alanine metabolism	51.72	15 of 29
[Ref.: #66794]	tyrosine metabolism	50	7 of 14
[Ref.: #66794]	propionate fermentation	50	5 of 10
[Ref.: #66794]	ethanol fermentation	50	1 of 2
[Ref.: #66794]	C4 and CAM-carbon fixation	50	4 of 8
[Ref.: #66794]	cis-vaccenate biosynthesis	50	1 of 2
[Ref.: #66794]	isoleucine metabolism	50	4 of 8
[Ref.: #66794]	dTDPLrhamnose biosynthesis	50	4 of 8
[Ref.: #66794]	citric acid cycle	50	7 of 14
[Ref.: #66794]	gluconeogenesis	50	4 of 8
[Ref.: #66794]	lactate fermentation	50	2 of 4
[Ref.: #66794]	quinate degradation	50	1 of 2
[Ref.: #66794]	methionine metabolism	46.15	12 of 26
[Ref.: #66794]	urea cycle	46.15	6 of 13
[Ref.: #66794]	leucine metabolism	46.15	6 of 13
[Ref.: #66794]	vitamin B6 metabolism	45.45	5 of 11
[Ref.: #66794]	lipid metabolism	45.16	14 of 31
[Ref.: #66794]	nitrate assimilation	44.44	4 of 9
[Ref.: #66794]	allantoin degradation	44.44	4 of 9
[Ref.: #66794]	chorismate metabolism	44.44	4 of 9
[Ref.: #66794]	glutathione metabolism	42.86	6 of 14
[Ref.: #66794]	degradation of aromatic, nitrogen containing compounds	41.67	5 of 12
[Ref.: #66794]	phenylacetate degradation (aerobic)	40	2 of 5
[Ref.: #66794]	vitamin K metabolism	40	2 of 5
[Ref.: #66794]	threonine metabolism	40	4 of 10
[Ref.: #66794]	degradation of hexoses	38.89	7 of 18
[Ref.: #66794]	histidine metabolism	37.93	11 of 29
[Ref.: #66794]	tryptophan metabolism	36.84	14 of 38
[Ref.: #66794]	pentose phosphate pathway	36.36	4 of 11
[Ref.: #66794]	degradation of pentoses	35.71	10 of 28
[Ref.: #66794]	lysine metabolism	33.33	14 of 42
[Ref.: #66794]	cyanate degradation	33.33	1 of 3
[Ref.: #66794]	formaldehyde oxidation	33.33	1 of 3
[Ref.: #66794]	glycolate and glyoxylate degradation	33.33	2 of 6
[Ref.: #66794]	arginine metabolism	33.33	8 of 24
[Ref.: #66794]	selenocysteine biosynthesis	33.33	2 of 6
[Ref.: #66794]	IAA biosynthesis	33.33	1 of 3
[Ref.: #66794]	degradation of sugar alcohols	31.25	5 of 16
[Ref.: #66794]	sulfate reduction	30.77	4 of 13
[Ref.: #66794]	glycine metabolism	30	3 of 10
[Ref.: #66794]	coenzyme M biosynthesis	30	3 of 10
[Ref.: #66794]	cysteine metabolism	27.78	5 of 18
[Ref.: #66794]	ascorbate metabolism	27.27	6 of 22
[Ref.: #66794]	metabolism of disaccharids	27.27	3 of 11
[Ref.: #66794]	3-phenylpropionate degradation	26.67	4 of 15
[Ref.: #66794]	polyamine pathway	26.09	6 of 23
[Ref.: #66794]	carnitine metabolism	25	2 of 8
[Ref.: #66794]	toluene degradation	25	1 of 4
[Ref.: #66794]	glycogen biosynthesis	25	1 of 4
[Ref.: #66794]	cyclohexanol degradation	25	1 of 4
[Ref.: #66794]	butanoate fermentation	25	1 of 4
[Ref.: #66794]	phenylpropanoid biosynthesis	23.08	3 of 13
[Ref.: #66794]	phosphatidylethanolamine bioynthesis	23.08	3 of 13
[Ref.: #66794]	4-hydroxymandelate degradation	22.22	2 of 9
		Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #65116]	Sample type/isolated from	Feces of man
[Ref.: #65116]	Geographic location (country and/or sea, region)	Tsukuba
[Ref.: #65116]	Country	Japan
[Ref.: #65116]	Country ISO 3 Code	JPN
[Ref.: #65116]	Continent	Asia

[Ref.: #66790]	Sample type/isolated from	faecal sample of a healthy Japanese man (37 years old)
[Ref.: #66790]	Sampling date	2017-02
[Ref.: #66790]	Geographic location (country and/or sea, region)	Tsukuba, Ibaraki
[Ref.: #66790]	Country	Japan
[Ref.: #66790]	Country ISO 3 Code	JPN
[Ref.: #66790]	Continent	Asia
[Ref.: #66790]	Enrichment culture	Brucella blood agar
[Ref.: #66790]	Enrichment culture composition	with haemin and menadione
[Ref.: #66790]	Enrichment culture duration	2-4 days
[Ref.: #66790]	Enrichment culture temperature	37 ̊C
[Ref.: #66790]	Isolation/enrichment procedure	Each dilution of the fresh faecal samples was plated onto Brucella blood agar and incubated for 2-4 days at 37°C under a H2/CO2/N2 (1:1:8, by vol.) gas mixture.

[Ref.: #67770]	Sample type/isolated from	Feces of a man in the thirties
[Ref.: #67770]	Country	Japan
[Ref.: #67770]	Country ISO 3 Code	JPN
[Ref.: #67770]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Host Body Product	#Gastrointestinal tract	#Feces (Stool)
#Host	#Human	#Male

What are isolation sources categories?

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #66790]	Sutterella megalosphaeroides 6FBBBH3 gene for 16S ribosomal RNA, partial sequence	LC388666	1472	ENA	2494234 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Sutterella megalosphaeroides DNA, complete genome	AP018786		ENA	2494234 tax ID
[Ref.: #66792]	Sutterella megalosphaeroides 6FBBBH3	GCA_003609995	complete	GenBank	2494234 tax ID	*
[Ref.: #66792]	Sutterella sp. 6FBBBH3	2259134.3	complete	PATRIC	2494234 tax ID	*
[Ref.: #66792]	Sutterella megalosphaeroides 6FBBBH3	2833028006	complete	JGI IMG/M	2494234 tax ID	*

[Ref.: #66790]

GC-content

62.8 mol%

genome sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Sutterella megalosphaeroides 6FBBBH3 NCBI: GCA_003609995.1
[Ref.: #69480]	anaerobic	yes	89.324	no
[Ref.: #69480]	thermophile	no	96.819	yes
[Ref.: #69480]	gram-positive	no	99.999	yes
[Ref.: #69480]	spore-forming	no	99.995	no
[Ref.: #69480]	motile	no	87.06	yes

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Sutterella megalosphaeroides 6FBBBH3 NCBI: GCA_003609995.1
[Ref.: #69481]	spore-forming	no	100	no

Availability in culture collections External links

[Ref.: #65116]

Culture collection no.

DSM 106861, JCM 32470

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Mesosutterella multiformis gen. nov., sp. nov., a member of the family Sutterellaceae and Sutterella megalosphaeroides sp. nov., isolated from human faeces.	Sakamoto M, Ikeyama N, Kunihiro T, Iino T, Yuki M, Ohkuma M	Int J Syst Evol Microbiol	10.1099/ijsem.0.003096	2018	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#65116

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 106861

#66790

Mitsuo Sakamoto, Nao Ikeyama, Tadao Kunihiro, Takao Iino, Masahiro Yuki, Moriya Ohkuma: Mesosutterella multiformis gen. nov., sp. nov., a member of the family Sutterellaceae and Sutterella megalosphaeroides sp. nov., isolated from human faeces. IJSEM 68: 3942 - 3950 2018 ( DOI 10.1099/ijsem.0.003096 , PubMed 30394865 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#66794

Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

* These data were automatically processed and therefore are not curated

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