Strain identifier

BacDive ID: 158433

Type strain: Yes

Species: Lactococcus termiticola

Strain Designation: NtB2

Strain history: S. Noda; Univ. of Yamanashi, Japan; NtB2.

NCBI tax ID(s): 2169526 (species)

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General

@ref: 65109

BacDive-ID: 158433

DSM-Number: 107259

keywords: genome sequence, 16S sequence, Bacteria, facultative anaerobe, mesophilic, Gram-positive, coccus-shaped, colony-forming

description: Lactococcus termiticola NtB2 is a facultative anaerobe, mesophilic, Gram-positive bacterium that forms circular colonies and was isolated from gut of termite Nasutitermes takasagoensis.

NCBI tax id

  • NCBI tax id: 2169526
  • Matching level: species

strain history

@refhistory
65109<- M. Sakamoto, RIKEN, Wako, Saitama, Japan; NtB2 <- S. Noda, University of Yamanashi, Kofu, Japan; NtB2
67770S. Noda; Univ. of Yamanashi, Japan; NtB2.

doi: 10.13145/bacdive158433.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Streptococcaceae
  • genus: Lactococcus
  • species: Lactococcus termiticola
  • full scientific name: Lactococcus termiticola Noda et al. 2018

@ref: 65109

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Streptococcaceae

genus: Lactococcus

species: Lactococcus termiticola

full scientific name: Lactococcus termiticola Noda et al. 2018

strain designation: NtB2

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
66772positive0.5-0.68 µm0.34-0.38 µmcoccus-shapedno
69480no96.186
69480positive100

colony morphology

  • @ref: 66772
  • colony size: 1-3 mm
  • colony color: cream
  • colony shape: circular
  • incubation period: 2 days
  • medium used: BHI agar

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
65109MRS MEDIUM (DSMZ Medium 11)yeshttps://mediadive.dsmz.de/medium/11Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water
66772BHI agaryes

culture temp

@refgrowthtypetemperaturerange
65109positivegrowth30mesophilic
66772nogrowth40thermophilic
66772positivegrowth20-30
67770positivegrowth30mesophilic

culture pH

@refabilitytypepH
66772nogrowth5
66772positivegrowth6-8

Physiology and metabolism

oxygen tolerance

  • @ref: 66772
  • oxygen tolerance: facultative anaerobe

spore formation

  • @ref: 69480
  • spore formation: no
  • confidence: 99.997

halophily

  • @ref: 66772
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 2 %(w/v)

observation

  • @ref: 66772
  • observation: The cell-wall peptidoglycan is type A3a, Lys-Gly-Ser-Ala2.

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6677216634raffinose-builds acid from
6677216988D-ribose-builds acid from
6677217716lactose-builds acid from
6677217992sucrose-builds acid from
6677227613amygdalin-builds acid from
6677228017starch-builds acid from
6677228053melibiose-builds acid from
6677228066gentiobiose-builds acid from
6677230849L-arabinose-builds acid from
6677265327D-xylose-builds acid from
6677212936D-galactose+builds acid from
6677215824D-fructose+builds acid from
6677216024D-mannose+builds acid from
6677216899D-mannitol+builds acid from
6677217057cellobiose+builds acid from
6677217268myo-inositol+builds acid from
6677217306maltose+builds acid from
6677217634D-glucose+builds acid from
6677217814salicin+builds acid from
6677224265gluconate+builds acid from
6677227082trehalose+builds acid from
6677259640N-acetylglucosamine+builds acid from
66772esculin ferric citrate+builds acid from
6677217309pectin+degradation
6677237166xylan+degradation
66772crystalline cellulose+degradation

enzymes

@refvalueactivityec
66772alpha-glucosidase-3.2.1.20
66772beta-glucosidase-3.2.1.21
66772esterase-
66772esterase Lipase (C 8)-
66772acid phosphatase+3.1.3.2
66772alkaline phosphatase+3.1.3.1
66772leucine arylamidase+3.4.11.1
66772naphthol-AS-BI-phosphohydrolase+

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    66772C19:0 iso1.2
    66772C18:02.4
    66772C14:02.7
    66772cyclo-C19:0 w8c2.9
    66772C16:025.7
    66772C18:1 w9c52.4
  • type of FA analysis: whole cell analysis
  • incubation temperature: 30
  • software version: Sherlock 6.2B
  • library/peak naming table: TSBA6
  • system: MIS MIDI

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentenrichment cultureenrichment culture durationenrichment culture temperatureisolation procedurehost species
65109gut of termite Nasutitermes takasagoensisOkinawa Prefecture, Iriomote IslandJapanJPNAsia
66772the gut of the wood-feeding higher termite Nasutitermes takasagoensisJapanJPNAsiabrain heart infusion (BHI; Difco)1 day30The gut content of N. takasagoensis was spread onto a brain heart infusion (BHI; Difco) agar plate and cultivated at 30 C for 1 day under anaerobic conditions by using the AnaeroPack system (Mitsubishi Gas Cemicals).
67770Gut of the wood-feeding higher termite Nasutitermes takasagoensisIriomote Island, Okinawa Pref.JapanJPNAsiaNasutitermes takasagoensis

isolation source categories

Cat1Cat2Cat3
#Host#Arthropoda#Insecta
#Host Body-Site#Gastrointestinal tract

Sequence information

16S sequences

  • @ref: 66772
  • description: Lactococcus termiticola NtB2 gene for 16S ribosomal RNA, partial sequence
  • accession: LC389879
  • length: 1472
  • database: ena
  • NCBI tax ID: 2169526

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Lactococcus sp. NtB22169526.3wgspatric2169526
66792Lactococcus termiticola NtB22882205748draftimg2169526
67770Lactococcus termiticola NtB2GCA_003116835contigncbi2169526

GC content

@refGC-contentmethod
6510947.5
6677247.5genome sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno84no
motileno94.84no
gram-positiveyes95.765yes
anaerobicno97.153yes
aerobicno96.82yes
halophileyes81.07no
spore-formingno96.585no
glucose-utilyes92.208no
thermophileno96.789no
flagellatedno98.66no
glucose-fermentyes87.402no

External links

@ref: 65109

culture collection no.: DSM 107259, JCM 32569

straininfo link

  • @ref: 111234
  • straininfo: 405159

literature

  • topic: Phylogeny
  • Pubmed-ID: 30320546
  • title: Lactococcus termiticola sp. nov., isolated from the gut of the wood-feeding higher termite Nasutitermes takasagoensis.
  • authors: Noda S, Sakamoto M, Aihara C, Yuki M, Katsuhara M, Ohkuma M
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.003068
  • year: 2018
  • mesh: Animals, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Genes, Bacterial, Isoptera/*microbiology, Lactococcus/*classification/genetics/isolation & purification, Peptidoglycan/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Wood
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
65109Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-107259Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 107259)
66772Satoko Noda, Mitsuo Sakamoto, Chihiro Aihara, Masahiro Yuki, Megumi Katsuhara and Moriya OhkumaLactococcus termiticola sp. nov., isolated from the gut of the wood-feeding higher termite Nasutitermes takasagoensis10.1099/ijsem.0.003068IJSEM 68: 3832-3836 201830320546
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar SchomburgBRENDA, the ELIXIR core data resource in 2021: new developments and updates10.1093/nar/gkaa1025Nucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
111234Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID405159.1