Lactococcus termiticola (DSM 107259, JCM 32569, NtB2)

Name: Lactococcus termiticola (DSM 107259, JCM 32569, NtB2)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 158433

Type strain

Strain Designation NtB2

Culture col. no. DSM 107259 JCM 32569 NtB2

NCBI tax ID(s) 2169526

Special Collections DSMZ JCM Literature strains

History <- M. Sakamoto, RIKEN, Wako, Saitama, Japan; NtB2 <- S. Noda, University of Yamanashi, Kofu, Japan; NtB2 S. Noda; Univ. of Yamanashi, Japan; NtB2.

Links

Lactococcus termiticola NtB2 is a facultative anaerobe, Gram-positive, coccus-shaped bacterium that forms circular colonies and was isolated from gut of termite Nasutitermes takasagoensis.

Gram-positive coccus-shaped colony-forming facultative anaerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 405159

JCM 32569,DSM 107259

@ref 20215

Last LPSN update 2026-02-09 11:38:19

Domain Bacteria

Phylum Bacillota

Class Bacilli

Order Lactobacillales

Family Streptococcaceae

Genus Lactococcus

Species Lactococcus termiticola

Full scientific name Lactococcus termiticola Noda et al. 2018

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Confidence
66772	positive	0.5-0.68 µm	0.34-0.38 µm	coccus-shaped
125438					95
125438	positive				92.302

Colony morphology

@ref	Colony size	Colony color	Colony shape	Incubation period	Medium used
66772	1-3 mm	cream	circular	2 days	BHI agar

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
65109	MRS MEDIUM (DSMZ Medium 11)		Medium recipe at MediaDive	Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water
66772	BHI agar

Culture temp

@ref	Growth	Type	Temperature (°C)
65109	positive	growth	30
66772	negative	growth	40
66772	positive	growth	20-30
67770	positive	growth	30

Culture pH

@ref	Ability	Type	PH
66772		growth	5
66772	positive	growth	6-8

Physiology and metabolism

Oxygen tolerance

66772

Oxygen tolerancefacultative anaerobe

Spore formation

@ref	Spore formation	Confidence
125439		98.5

Halophily

@ref	Salt	Growth	Tested relation	Concentration
66772	NaCl	positive	growth	2 %(w/v)

Observation

66772

ObservationThe cell-wall peptidoglycan is type A3a, Lys-Gly-Ser-Ala2.

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
66772	27613 ChEBI	amygdalin	-	builds acid from
66772	17057 ChEBI	cellobiose	+	builds acid from
66772		crystalline cellulose	+	degradation
66772	15824 ChEBI	D-fructose	+	builds acid from
66772	12936 ChEBI	D-galactose	+	builds acid from
66772	17634 ChEBI	D-glucose	+	builds acid from
66772	16899 ChEBI	D-mannitol	+	builds acid from
66772	16024 ChEBI	D-mannose	+	builds acid from
66772	16988 ChEBI	D-ribose	-	builds acid from
66772	65327 ChEBI	D-xylose	-	builds acid from
66772		esculin ferric citrate	+	builds acid from
66772	28066 ChEBI	gentiobiose	-	builds acid from
66772	24265 ChEBI	gluconate	+	builds acid from
66772	30849 ChEBI	L-arabinose	-	builds acid from
66772	17716 ChEBI	lactose	-	builds acid from
66772	17306 ChEBI	maltose	+	builds acid from
66772	28053 ChEBI	melibiose	-	builds acid from
66772	17268 ChEBI	myo-inositol	+	builds acid from
66772	59640 ChEBI	N-acetylglucosamine	+	builds acid from
66772	17309 ChEBI	pectin	+	degradation
66772	16634 ChEBI	raffinose	-	builds acid from
66772	17814 ChEBI	salicin	+	builds acid from
66772	28017 ChEBI	starch	-	builds acid from
66772	17992 ChEBI	sucrose	-	builds acid from
66772	27082 ChEBI	trehalose	+	builds acid from
66772	37166 ChEBI	xylan	+	degradation

Enzymes

@ref	Value	Activity	Ec
66772	acid phosphatase	+	3.1.3.2
66772	alkaline phosphatase	+	3.1.3.1
66772	alpha-glucosidase	-	3.2.1.20
66772	beta-glucosidase	-	3.2.1.21
66772	esterase	-
66772	esterase Lipase (C 8)	-
66772	leucine arylamidase	+	3.4.11.1
66772	naphthol-AS-BI-phosphohydrolase	+

Pathway annotation

Computationally determined by

@ref	pathway	enzyme coverage	annotated reactions
66794	cis-vaccenate biosynthesis	100	2 of 2
66794	acetoin degradation	100	3 of 3
66794	vitamin K metabolism	100	5 of 5
66794	formaldehyde oxidation	100	3 of 3
66794	coenzyme A metabolism	100	4 of 4
66794	CDP-diacylglycerol biosynthesis	100	2 of 2
66794	folate polyglutamylation	100	1 of 1
66794	UDP-GlcNAc biosynthesis	100	3 of 3
66794	anapleurotic synthesis of oxalacetate	100	1 of 1
66794	palmitate biosynthesis	100	22 of 22
66794	peptidoglycan biosynthesis	93.33	14 of 15
66794	chorismate metabolism	88.89	8 of 9
66794	pentose phosphate pathway	81.82	9 of 11
66794	glycogen metabolism	80	4 of 5
66794	molybdenum cofactor biosynthesis	77.78	7 of 9
66794	phenylalanine metabolism	76.92	10 of 13
66794	dTDPLrhamnose biosynthesis	75	6 of 8
66794	sulfopterin metabolism	75	3 of 4
66794	ppGpp biosynthesis	75	3 of 4
66794	glycogen biosynthesis	75	3 of 4
66794	acetate fermentation	75	3 of 4
66794	cardiolipin biosynthesis	71.43	5 of 7
66794	photosynthesis	71.43	10 of 14
66794	reductive acetyl coenzyme A pathway	71.43	5 of 7
66794	purine metabolism	70.21	66 of 94
66794	starch degradation	70	7 of 10
66794	valine metabolism	66.67	6 of 9
66794	serine metabolism	66.67	6 of 9
66794	enterobactin biosynthesis	66.67	2 of 3
66794	aspartate and asparagine metabolism	66.67	6 of 9
66794	L-lactaldehyde degradation	66.67	2 of 3
66794	NAD metabolism	66.67	12 of 18
66794	glycolysis	64.71	11 of 17
66794	pyrimidine metabolism	64.44	29 of 45
66794	ketogluconate metabolism	62.5	5 of 8
66794	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	62.5	5 of 8
66794	vitamin B1 metabolism	61.54	8 of 13
66794	methionine metabolism	61.54	16 of 26
66794	lipoate biosynthesis	60	3 of 5
66794	glycine betaine biosynthesis	60	3 of 5
66794	myo-inositol biosynthesis	60	6 of 10
66794	Entner Doudoroff pathway	60	6 of 10
66794	heme metabolism	57.14	8 of 14
66794	degradation of sugar alcohols	56.25	9 of 16
66794	oxidative phosphorylation	56.04	51 of 91
66794	CO2 fixation in Crenarchaeota	55.56	5 of 9
66794	d-xylose degradation	54.55	6 of 11
66794	alanine metabolism	51.72	15 of 29
66794	isoprenoid biosynthesis	50	13 of 26
66794	ethanol fermentation	50	1 of 2
66794	glycolate and glyoxylate degradation	50	3 of 6
66794	suberin monomers biosynthesis	50	1 of 2
66794	adipate degradation	50	1 of 2
66794	lactate fermentation	50	2 of 4
66794	butanoate fermentation	50	2 of 4
66794	isoleucine metabolism	50	4 of 8
66794	tetrahydrofolate metabolism	50	7 of 14
66794	non-pathway related	47.37	18 of 38
66794	proline metabolism	45.45	5 of 11
66794	lipid metabolism	45.16	14 of 31
66794	d-mannose degradation	44.44	4 of 9
66794	cysteine metabolism	44.44	8 of 18
66794	glutamate and glutamine metabolism	42.86	12 of 28
66794	propanol degradation	42.86	3 of 7
66794	mevalonate metabolism	42.86	3 of 7
66794	metabolism of amino sugars and derivatives	40	2 of 5
66794	3-chlorocatechol degradation	40	2 of 5
66794	arachidonate biosynthesis	40	2 of 5
66794	glycine metabolism	40	4 of 10
66794	threonine metabolism	40	4 of 10
66794	leucine metabolism	38.46	5 of 13
66794	urea cycle	38.46	5 of 13
66794	C4 and CAM-carbon fixation	37.5	3 of 8
66794	gluconeogenesis	37.5	3 of 8
66794	vitamin B6 metabolism	36.36	4 of 11
66794	degradation of pentoses	35.71	10 of 28
66794	tryptophan metabolism	34.21	13 of 38
66794	acetyl CoA biosynthesis	33.33	1 of 3
66794	degradation of aromatic, nitrogen containing compounds	33.33	4 of 12
66794	cyanate degradation	33.33	1 of 3
66794	octane oxidation	33.33	1 of 3
66794	IAA biosynthesis	33.33	1 of 3
66794	lipid A biosynthesis	33.33	3 of 9
66794	degradation of hexoses	33.33	6 of 18
66794	flavin biosynthesis	33.33	5 of 15
66794	selenocysteine biosynthesis	33.33	2 of 6
66794	degradation of sugar acids	32	8 of 25
66794	ascorbate metabolism	31.82	7 of 22
66794	coenzyme M biosynthesis	30	3 of 10
66794	tyrosine metabolism	28.57	4 of 14
66794	ubiquinone biosynthesis	28.57	2 of 7
66794	metabolism of disaccharids	27.27	3 of 11
66794	biotin biosynthesis	25	1 of 4
66794	cyclohexanol degradation	25	1 of 4
66794	androgen and estrogen metabolism	25	4 of 16
66794	CMP-KDO biosynthesis	25	1 of 4
66794	arginine metabolism	25	6 of 24
66794	sulfate reduction	23.08	3 of 13
66794	arachidonic acid metabolism	22.22	4 of 18
66794	nitrate assimilation	22.22	2 of 9
66794	glutathione metabolism	21.43	3 of 14
66794	histidine metabolism	20.69	6 of 29

Fatty acid profile

Metadata FA analysis

@ref

66772

Fatty acid	Percentage
C18:1 w9c	52.4
C16:0	25.7
cyclo-C19:0 w8c	2.9
C14:0	2.7
C18:0	2.4
C19:0 iso	1.2

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host	#Arthropoda	#Insecta
#Host Body-Site	#Gastrointestinal tract	-

Isolation

@ref	Sample type	Country	Country ISO 3 Code	Continent	Enrichment culture	Enrichment culture duration	Enrichment culture temperature	Isolation procedure	Geographic location	Host species
65109	gut of termite Nasutitermes takasagoensis	Japan	JPN	Asia					Okinawa Prefecture, Iriomote Island
66772	the gut of the wood-feeding higher termite Nasutitermes takasagoensis	Japan	JPN	Asia	brain heart infusion (BHI; Difco)	1 day	30	The gut content of N. takasagoensis was spread onto a brain heart infusion (BHI; Difco) agar plate and cultivated at 30 C for 1 day under anaerobic conditions by using the AnaeroPack system (Mitsubishi Gas Cemicals).
67770	Gut of the wood-feeding higher termite Nasutitermes takasagoensis	Japan	JPN	Asia					Iriomote Island, Okinawa Pref.	Nasutitermes takasagoensis

Interaction and safety

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	ASM311683v1 assembly for Lactococcus termiticola NtB2	contig	GCA_003116835	2169526.3	2882205748	2169526	70.62

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
66772	Lactococcus termiticola NtB2 gene for 16S ribosomal RNA, partial sequence	LC389879	1472		2169526

GC content

@ref	GC-content (mol%)	Method
65109	47.5
66772	47.5	genome sequence analysis

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Lactococcus termiticola NtB2
NCBI: GCA_003116835

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	98.50	no
125439	motility	BacteriaNetⓘ	no	75.40	no
125439	gram_stain	BacteriaNetⓘ	negative	72.00	no
125439	oxygen_tolerance	BacteriaNetⓘ	anaerobe	84.10	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Lactococcus sp. NtB2
PATRIC: 2169526.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	92.30	no
125438	anaerobic	anaerobicⓘ	no	81.94	yes
125438	spore-forming	spore-formingⓘ	no	86.68	no
125438	thermophilic	thermophileⓘ	no	96.47	yes
125438	aerobic	aerobicⓘ	no	93.42	yes
125438	flagellated	motile2+ⓘ	no	95.00	yes

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Lactococcus termiticola sp. nov., isolated from the gut of the wood-feeding higher termite Nasutitermes takasagoensis.	Noda S, Sakamoto M, Aihara C, Yuki M, Katsuhara M, Ohkuma M	Int J Syst Evol Microbiol	10.1099/ijsem.0.003068	2018

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#65109	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 107259
#66772	Satoko Noda, Mitsuo Sakamoto, Chihiro Aihara, Masahiro Yuki, Megumi Katsuhara and Moriya Ohkuma: Lactococcus termiticola sp. nov., isolated from the gut of the wood-feeding higher termite Nasutitermes takasagoensis. IJSEM 68: 3832 - 3836 2018 ( DOI 10.1099/ijsem.0.003068 , PubMed 30320546 )
#66794	Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Lactococcus termiticola (DSM 107259, JCM 32569, NtB2)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Observation

Metabolite utilization

Enzymes

Pathway annotation

Fatty acid profile

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Sequence information

Genome sequences

Genome browser: GCA_003116835

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

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