Strain identifier

BacDive ID: 158416

Type strain: Yes

Species: Peribacillus acanthi

Strain Designation: L28

Strain history: <- Q. Yin; Shenzhen University, Shenzhen, P.R. CHINA; L28

NCBI tax ID(s): 2171554 (species)

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General

@ref: 65092

BacDive-ID: 158416

DSM-Number: 104296

keywords: genome sequence, 16S sequence, Bacteria, aerobe, spore-forming, Gram-positive, rod-shaped, colony-forming

description: Peribacillus acanthi L28 is an aerobe, spore-forming, Gram-positive bacterium that forms circular colonies and was isolated from Mai Po Inner Deep Bay rhizospere of Acanthus ilicifolius.

NCBI tax id

  • NCBI tax id: 2171554
  • Matching level: species

strain history

  • @ref: 65092
  • history: <- Q. Yin; Shenzhen University, Shenzhen, P.R. CHINA; L28

doi: 10.13145/bacdive158416.20240916.9.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Peribacillus
  • species: Peribacillus acanthi
  • full scientific name: Peribacillus acanthi (Ma et al. 2018) Patel and Gupta 2020

  • synonyms

    • @ref: 20215
    • synonym: Bacillus acanthi

@ref: 65092

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Bacillaceae

genus: Peribacillus

species: Peribacillus acanthi

full scientific name: Peribacillus acanthi (Ma et al. 2018) Patel and Gupta 2020

strain designation: L28

type strain: yes

Morphology

cell morphology

  • @ref: 66677
  • gram stain: positive
  • cell length: 1.5-5.0 µm
  • cell width: 0.5-1.0 µm
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 66677
  • colony size: 0.5-5 mm
  • colony color: white pigmented
  • colony shape: circular
  • incubation period: 3 days
  • medium used: Marine agar (MA)

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
65092BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)yeshttps://mediadive.dsmz.de/medium/514Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water
66677Marine agar (MA)yes

culture temp

@refgrowthtypetemperature
65092positivegrowth30
66677positiveoptimum37
66677positivegrowth20-45

culture pH

@refabilitytypepHPH range
66677positiveoptimum7.5
66677positivegrowth6.5-9.0alkaliphile

Physiology and metabolism

oxygen tolerance

  • @ref: 66677
  • oxygen tolerance: aerobe

spore formation

@reftype of sporespore formationconfidence
66677endosporeyes
69480yes93.932
69481yes100

halophily

@refsaltgrowthtested relationconcentration
66677NaClpositivegrowth0-5 %(w/v)
66677NaClpositiveoptimum0.5 %(w/v)

observation

  • @ref: 66677
  • observation: colonies are circular, smooth, convex and regular withentire margin.

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6667753258citric acid+assimilation
6667727897tryptophan+energy source
6667717234glucose-fermentation
6667717268myo-inositol-fermentation
6667717992sucrose-fermentation
6667722599arabinose-fermentation
6667726546rhamnose-fermentation
6667727613amygdalin-fermentation
6667728053melibiose-fermentation
6667730911sorbitol-fermentation
6667737684mannose-fermentation
666775291gelatin-hydrolysis
6667716199urea-hydrolysis
6667753423tween 40-hydrolysis
6667753424tween 20-hydrolysis
6667753425tween 60-hydrolysis
6667753426tween 80-hydrolysis
66677casein-hydrolysis
6667728017starch+hydrolysis
6667717632nitrate-reduction

metabolite production

@refChebi-IDmetaboliteproduction
6667716136hydrogen sulfideyes
6667715688acetoinno
6667735581indoleno

enzymes

@refvalueactivityec
66677alpha-chymotrypsin-3.4.21.1
66677alpha-fucosidase-3.2.1.51
66677alpha-glucosidase-3.2.1.20
66677alpha-mannosidase-3.2.1.24
66677arginine dihydrolase-3.5.3.6
66677beta-galactosidase-3.2.1.23
66677beta-glucosidase-3.2.1.21
66677beta-glucuronidase-3.2.1.31
66677cystine arylamidase-3.4.11.3
66677esterase-
66677esterase (C 4)-
66677gelatinase-
66677leucine arylamidase-3.4.11.1
66677lipase (C 14)-
66677lysine decarboxylase-4.1.1.18
66677ornithine decarboxylase-4.1.1.17
66677trypsin-3.4.21.4
66677tryptophan deaminase-4.1.99.1
66677urease-3.5.1.5
66677valine arylamidase-
66677acid phosphatase+3.1.3.2
66677alkaline phosphatase+3.1.3.1
66677alpha-galactosidase+3.2.1.22
66677beta-N-acetylgalactosaminidase+
66677catalase+1.11.1.6
66677cytochrome oxidase+1.9.3.1
66677esterase Lipase (C 8)+
66677naphthol-AS-BI-phosphohydrolase+
66677tryptophan deaminase+4.1.99.1

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentgeographic locationlatitudelongitudeenrichment cultureisolation procedure
65092Mai Po Inner Deep Bay rhizospere of Acanthus ilicifoliusChinaCHNAsia
66677the rhizosphere soil of a mangrove plant, Acanthus ilicifoliusMai Po Inner Deep Bay Ramsar Site22.49114.05sterile marine agar 2216the conventional dilution-plating technique

isolation source categories

Cat1Cat2Cat3
#Host#Plants#Herbaceous plants (Grass,Crops)
#Host Body-Site#Plant#Rhizosphere
#Environmental#Terrestrial#Soil
#Environmental#Terrestrial#Wetland (Swamp)

Sequence information

16S sequences

  • @ref: 65092
  • description: Peribacillus acanthi strain L28 16S ribosomal RNA gene, partial sequence
  • accession: KY038378
  • length: 1521
  • database: nuccore
  • NCBI tax ID: 2171554

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Peribacillus acanthi L28GCA_003073175scaffoldncbi2171554
66792Bacillus sp. XY-L282171554.3wgspatric2171554

GC content

@refGC-contentmethod
6509236.9
6667736.93genome sequence analysis

Genome-based predictions

predictions

@reftraitmodeldescriptionpredictionconfidencetraining_data
69481spore-formingspore-formingAbility to form endo- or exosporesyes100no
69480gram-positivegram-positivePositive reaction to Gram-stainingyes73.298no
69480anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)no94.708yes
69480aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)yes79.274yes
69480spore-formingspore-formingAbility to form endo- or exosporesyes93.932yes
69480thermophilicthermophileAbility to grow at temperatures above or equal to 45°Cno87.733yes
69480flagellatedmotile2+Ability to perform flagellated movementyes82.692no

External links

@ref: 65092

culture collection no.: DSM 104296, MCCC 1K03287

straininfo link

  • @ref: 111219
  • straininfo: 400102

literature

  • topic: Phylogeny
  • Pubmed-ID: 30063204
  • title: Bacillus acanthi sp. nov., isolated from the rhizosphere soil of a mangrove plant Acanthus ilicifolius.
  • authors: Ma K, Yin Q, Chen L, Lai Q, Xu Y
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.002950
  • year: 2018
  • mesh: Acanthaceae/*microbiology, Bacillus/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Hong Kong, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, *Rhizosphere, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/analogs & derivatives/chemistry
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
65092Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-104296Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 104296)
66677Ke Ma, Qi Yin, Liying Chen, Qiliang Lai, Ying XuBacillus acanthi sp. nov., isolated from the rhizosphere soil of a mangrove plant Acanthus ilicifolius10.1099/ijsem.0.002950IJSEM 68: 3047-3051 201830063204
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1
111219Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID400102.1