Strain identifier

BacDive ID: 158395

Type strain: Yes

Species: Marinicrinis lubricantis

Strain Designation: KSS164-79

Strain history: CIP <- 2017, P. Kämpfer, Giessen Univ., Giessen, Germany: strain KSS 164-79, Paenibacillus sp.

NCBI tax ID(s): 2086470 (species)

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General

@ref: 65071

BacDive-ID: 158395

DSM-Number: 104943

keywords: 16S sequence, Bacteria, aerobe, spore-forming, mesophilic, Gram-positive, rod-shaped, colony-forming

description: Marinicrinis lubricantis KSS164-79 is an aerobe, spore-forming, mesophilic bacterium that forms circular colonies and was isolated from metalworking fluid .

NCBI tax id

  • NCBI tax id: 2086470
  • Matching level: species

strain history

@refhistory
65071<- P. Kaempfer; Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, Germany; KSS164-79
120658CIP <- 2017, P. Kämpfer, Giessen Univ., Giessen, Germany: strain KSS 164-79, Paenibacillus sp.

doi: 10.13145/bacdive158395.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Paenibacillaceae
  • genus: Marinicrinis
  • species: Marinicrinis lubricantis
  • full scientific name: Marinicrinis lubricantis Kämpfer et al. 2018

@ref: 65071

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Caryophanales

family: Paenibacillaceae

genus: Marinicrinis

species: Marinicrinis lubricantis

full scientific name: Marinicrinis lubricantis Kämpfer et al. 2018

strain designation: KSS164-79

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotility
65441positive2-5 µm1 µmrod-shapedno
120658positiverod-shapedno

colony morphology

  • @ref: 65441
  • colony color: beige
  • colony shape: circular
  • medium used: nutrient agar

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
65071TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
65441brain heart infusion agaryes
65441Trypticase soy agaryes
65441Reasoner's 2A agaryes
65441Nutrient agar (NA)yes
120658CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
65071positivegrowth30mesophilic
65441positivegrowth25-45
65441nogrowth0-19.9psychrophilic
65441nogrowth50.1-100hyperthermophilic
65441positiveoptimum28-37mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 65441
  • oxygen tolerance: aerobe

spore formation

  • @ref: 65441
  • type of spore: endospore
  • spore formation: yes

halophily

@refsaltgrowthtested relationconcentration
65441NaClpositivegrowth0.5-7.0 %(w/v)
65441NaClpositiveoptimum1-2 %(w/v)

observation

  • @ref: 65441
  • observation: the quinone system contains predominantly MK-7

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
654412-deoxythymidine-5'-4-nitrophenyl phosphate-hydrolysis
654412-oxoglutarate-carbon source30916
65441gamma-aminobutyric acid-carbon source16865
654414-hydroxybenzoate-carbon source17879
654414-nitrophenyl alpha-D-glucopyranoside+hydrolysis91122
654414-nitrophenyl beta-D-galactopyranoside-hydrolysis355715
654414-nitrophenyl beta-D-galactopyranoside+hydrolysis355715
654414-nitrophenyl beta-D-glucuronide-hydrolysis90146
654414-nitrophenyl beta-D-xylopyranoside-hydrolysis90148
65441adipate-carbon source17128
65441ribitol-builds acid from15963
65441ribitol-carbon source15963
65441azelaate-carbon source78208
65441beta-alanine-carbon source16958
65441bis-4-nitrophenyl phosphate-hydrolysis3122
65441bis-4-nitrophenyl-phenyl phosphonate-hydrolysis91050
65441bis-4-nitrophenyl-phosphorylcholine-hydrolysis55394
65441casein-hydrolysis
65441cellobiose-carbon source17057
65441cis-aconitate-carbon source16383
65441D-arabitol-builds acid from18333
65441D-fructose-carbon source15824
65441D-galactose-carbon source12936
65441D-glucose+builds acid from17634
65441D-glucose+carbon source17634
65441D-mannitol-builds acid from16899
65441D-mannitol-carbon source16899
65441D-mannose-carbon source16024
65441D-ribose-carbon source16988
65441D-sorbitol-builds acid from17924
65441D-sorbitol-carbon source17924
65441D-xylose-builds acid from65327
65441D-xylose+carbon source65327
654413-hydroxybutyrate-carbon source37054
65441lactate-carbon source24996
65441dna-hydrolysis16991
65441galactitol-builds acid from16813
65441erythritol-builds acid from17113
65441fumarate-carbon source29806
65441gamma-L-glutamate-4-nitroanilide-hydrolysis
65441gelatin-hydrolysis5291
65441gluconate-carbon source24265
65441glutarate-carbon source30921
65441glycerol 3-phosphate-carbon source15978
65441itaconate-carbon source17240
65441L-alanine 4-nitroanilide-carbon source
65441L-alanine 4-nitroanilide-hydrolysis
65441L-arabinose-builds acid from30849
65441L-arabinose+carbon source30849
65441L-aspartate-carbon source29991
65441L-histidine-carbon source15971
65441L-leucine-carbon source15603
65441L-ornithine-carbon source15729
65441L-phenylalanine-carbon source17295
65441L-proline-carbon source17203
65441L-proline-4-nitroanilide-hydrolysis
65441L-rhamnose-builds acid from62345
65441L-rhamnose-carbon source62345
65441L-serine-carbon source17115
65441L-tryptophan-carbon source16828
65441lactose-builds acid from17716
65441maltitol-carbon source68428
65441maltose-builds acid from17306
65441melibiose-builds acid from28053
65441mesaconate-carbon source36986
65441myo-inositol-builds acid from17268
65441myo-inositol-carbon source17268
65441methyl alpha-D-glucopyranoside-builds acid from320061
65441N-acetylgalactosamine-carbon source28037
65441N-acetylglucosamine-carbon source506227
65441phenylacetate-carbon source18401
65441propionate-carbon source17272
65441putrescine-carbon source17148
65441pyruvate-carbon source15361
65441raffinose-builds acid from16634
65441salicin-builds acid from17814
65441salicin-carbon source17814
65441sodium acetate-carbon source32954
65441sodium citrate-carbon source53258
65441starch-hydrolysis28017
65441suberic acid-carbon source9300
65441sucrose-builds acid from17992
65441sucrose-carbon source17992
65441trans-aconitate-carbon source15708
65441trehalose-builds acid from27082
65441tyrosine-hydrolysis18186

metabolite production

@refChebi-IDmetaboliteproduction
6544116136hydrogen sulfideno
6544135581indoleno

enzymes

@refvalueactivityec
65441cytochrome oxidase+1.9.3.1
65441catalase+1.11.1.6
65441urease-3.5.1.5
65441lysine decarboxylase-4.1.1.18
65441ornithine decarboxylase-4.1.1.17
65441beta-galactosidase-3.2.1.23

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    65441C14:03
    65441C15:0 iso17.9
    65441C15:0 anteiso46.8
    65441C16:0 iso15.7
    65441C16:07.1
    65441C17:0 iso3.4
    65441C17:0 anteiso7.1
  • type of FA analysis: whole cell analysis
  • incubation medium: Trypticase soy agar
  • incubation temperature: 28
  • incubation time: 2
  • software version: Sherlock 2.11
  • library/peak naming table: TSBA4.1
  • system: MIS MIDI
  • instrument: HP 6890 gas chromatograph
  • method/protocol: Kämpfer & Kroppenstedt 1996
  • cutoff value:

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentenrichment cultureenrichment culture temperatureisolation procedureisolation date
65071metalworking fluid (coolant lubricant)Hessen, GießenGermanyDEUEurope
65441a coolant lubricantGermanyDEUEuropenutrient agar30the strain was initially isolated on nutrient agar (NA; Sigma-Aldrich) at 30C and also further maintained and subcultivated on this agar at 30C for 48 h
120658Environment, Metalworking fluidGiessen, HessenGermanyDEUEurope2016-12-30

isolation source categories

Cat1Cat2Cat3
#Engineered#Industrial#Industrial production
#Environmental#Aquatic

Safety information

risk assessment

  • @ref: 120658
  • biosafety level: 1
  • biosafety level comment: Risk group (French classification)

Sequence information

16S sequences

  • @ref: 65071
  • description: Marinicrinis lubricantis strain KSS164-79 16S ribosomal RNA gene, partial sequence
  • accession: MG589643
  • length: 1463
  • database: ena
  • NCBI tax ID: 2086470

GC content

@refGC-contentmethod
6507162.8
6544162.8high performance liquid chromatography (HPLC)

External links

@ref: 65071

culture collection no.: DSM 104943, CCM 8749, CIP 111345, LMG 30062

straininfo link

  • @ref: 111199
  • straininfo: 404345

literature

  • topic: Phylogeny
  • Pubmed-ID: 29458483
  • title: Marinicrinis lubricantis sp. nov., isolated from a coolant lubricant.
  • authors: Kampfer P, Busse HJ, Glaeser SP
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.002603
  • year: 2018
  • mesh: Bacillales/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Germany, Glycolipids/chemistry, *Lubricants, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistry
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
65071Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-104943Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 104943)
65441Peter Kämpfer, Hans-Jürgen Busse, Stefanie P. GlaeserMarinicrinis lubricantis sp. nov., isolated from a coolant lubricant10.1099/ijsem.0.002603IJSEM 68: 1018-1022 201829458483
111199Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID404345.1
120658Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20111345Collection of Institut Pasteur (CIP 111345)