Name: Marinicrinis lubricantis KSS164-79
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 158395

Type strain:

Species: Marinicrinis lubricantis

Strain Designation: KSS164-79

Culture col. no.: DSM 104943, CCM 8749, CIP 111345, LMG 30062

Strain history: CIP <- 2017, P. Kämpfer, Giessen Univ., Giessen, Germany: strain KSS 164-79, Paenibacillus sp.

NCBI tax ID(s): 2086470 (species)

SI-ID 404345

DSM 104943, CIP 111345

Section

Marinicrinis lubricantis KSS164-79 is an aerobe, spore-forming, mesophilic bacterium that forms circular colonies and was isolated from metalworking fluid .

colony-forming
Gram-positive
rod-shaped
spore-forming
aerobe
mesophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Paenibacillaceae*
	Genus	*Marinicrinis*
	Species	**Marinicrinis lubricantis**
	Full Scientific Name (LPSN)	Marinicrinis lubricantis Kämpfer et al. 2018

Information on morphological and physiological properties Morphology

[Ref.: #65441]	Gram stain	positive
[Ref.: #120658]	Gram stain	positive

[Ref.: #65441]	Cell length	2-5 µm

[Ref.: #65441]	Cell width	1 µm

[Ref.: #65441]	Cell shape	rod-shaped
[Ref.: #120658]	Cell shape	rod-shaped

[Ref.: #65441]	Motility	no
[Ref.: #120658]	Motility	no

[Ref.: #65441]	Colony color	beige
[Ref.: #65441]	Colony shape	circular
[Ref.: #65441]	Cultivation medium used	nutrient agar

Information on culture and growth conditions Culture and growth conditions

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Culture medium

TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

[Ref.: #65071]

Culture medium composition

expand / minimize

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Culture medium

brain heart infusion agar

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Culture medium growth

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Culture medium

Trypticase soy agar

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Culture medium growth

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Culture medium

Reasoner's 2A agar

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Culture medium growth

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Culture medium

Nutrient agar (NA)

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Culture medium growth

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Culture medium

CIP Medium 72

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Culture medium growth

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Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #65071]		growth	30 °C
[Ref.: #65441]		growth	25-45 °C
[Ref.: #65441]	no	growth	0-19.9 °C
[Ref.: #65441]	no	growth	50.1-100 °C
[Ref.: #65441]		optimum	28-37 °C

[Ref.: #65071]	Temperature range	mesophilic
[Ref.: #65441]	Temperature range	psychrophilic
[Ref.: #65441]	Temperature range	hyperthermophilic
[Ref.: #65441]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

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Oxygen tolerance

aerobe

[Ref.: #65441]	Type of spore	endospore
[Ref.: #65441]	Ability of spore formation	yes

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #65441]		NaCl		growth	0.5-7.0	%(w/v)
[Ref.: #65441]		NaCl		optimum	1-2	%(w/v)

[Ref.: #65441]

Observation

the quinone system contains predominantly MK-7

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #65441]	2-deoxythymidine-5'-4-nitrophenyl phosphate	-	hydrolysis
[Ref.: #65441]	2-oxoglutarate ChEBI	-	carbon source
[Ref.: #65441]	3-hydroxybutyrate ChEBI	-	carbon source
[Ref.: #65441]	4-hydroxybenzoate ChEBI	-	carbon source
[Ref.: #65441]	4-nitrophenyl alpha-D-glucopyranoside ChEBI	+	hydrolysis
[Ref.: #65441]	4-nitrophenyl beta-D-galactopyranoside ChEBI	-	hydrolysis
[Ref.: #65441]	4-nitrophenyl beta-D-galactopyranoside ChEBI	+	hydrolysis
[Ref.: #65441]	4-nitrophenyl beta-D-glucuronide ChEBI	-	hydrolysis
[Ref.: #65441]	4-nitrophenyl beta-D-xylopyranoside ChEBI	-	hydrolysis
[Ref.: #65441]	adipate ChEBI	-	carbon source
[Ref.: #65441]	azelaate ChEBI	-	carbon source
[Ref.: #65441]	beta-alanine ChEBI	-	carbon source
[Ref.: #65441]	bis-4-nitrophenyl phosphate ChEBI	-	hydrolysis
[Ref.: #65441]	bis-4-nitrophenyl-phenyl phosphonate ChEBI	-	hydrolysis
[Ref.: #65441]	bis-4-nitrophenyl-phosphorylcholine ChEBI	-	hydrolysis
[Ref.: #65441]	casein	-	hydrolysis
[Ref.: #65441]	cellobiose ChEBI	-	carbon source
[Ref.: #65441]	cis-aconitate ChEBI	-	carbon source
[Ref.: #65441]	D-arabitol ChEBI	-	builds acid from
[Ref.: #65441]	D-fructose ChEBI	-	carbon source
[Ref.: #65441]	D-galactose ChEBI	-	carbon source
[Ref.: #65441]	D-glucose ChEBI	+	builds acid from
[Ref.: #65441]	D-glucose ChEBI	+	carbon source
[Ref.: #65441]	D-mannitol ChEBI	-	builds acid from
[Ref.: #65441]	D-mannitol ChEBI	-	carbon source
[Ref.: #65441]	D-mannose ChEBI	-	carbon source
[Ref.: #65441]	D-ribose ChEBI	-	carbon source
[Ref.: #65441]	D-sorbitol ChEBI	-	builds acid from
[Ref.: #65441]	D-sorbitol ChEBI	-	carbon source
[Ref.: #65441]	D-xylose ChEBI	-	builds acid from
[Ref.: #65441]	D-xylose ChEBI	+	carbon source
[Ref.: #65441]	dna ChEBI	-	hydrolysis
[Ref.: #65441]	erythritol ChEBI	-	builds acid from
[Ref.: #65441]	fumarate ChEBI	-	carbon source
[Ref.: #65441]	galactitol ChEBI	-	builds acid from
[Ref.: #65441]	gamma-aminobutyric acid ChEBI	-	carbon source
[Ref.: #65441]	gamma-L-glutamate-4-nitroanilide	-	hydrolysis
[Ref.: #65441]	gelatin ChEBI	-	hydrolysis
[Ref.: #65441]	gluconate ChEBI	-	carbon source
[Ref.: #65441]	glutarate ChEBI	-	carbon source
[Ref.: #65441]	glycerol 3-phosphate ChEBI	-	carbon source
[Ref.: #65441]	itaconate ChEBI	-	carbon source
[Ref.: #65441]	L-alanine 4-nitroanilide	-	carbon source
[Ref.: #65441]	L-alanine 4-nitroanilide	-	hydrolysis
[Ref.: #65441]	L-arabinose ChEBI	-	builds acid from
[Ref.: #65441]	L-arabinose ChEBI	+	carbon source
[Ref.: #65441]	L-aspartate ChEBI	-	carbon source
[Ref.: #65441]	L-histidine ChEBI	-	carbon source
[Ref.: #65441]	L-leucine ChEBI	-	carbon source
[Ref.: #65441]	L-ornithine ChEBI	-	carbon source
[Ref.: #65441]	L-phenylalanine ChEBI	-	carbon source
[Ref.: #65441]	L-proline ChEBI	-	carbon source
[Ref.: #65441]	L-proline-4-nitroanilide	-	hydrolysis
[Ref.: #65441]	L-rhamnose ChEBI	-	builds acid from
[Ref.: #65441]	L-rhamnose ChEBI	-	carbon source
[Ref.: #65441]	L-serine ChEBI	-	carbon source
[Ref.: #65441]	L-tryptophan ChEBI	-	carbon source
[Ref.: #65441]	lactate ChEBI	-	carbon source
[Ref.: #65441]	lactose ChEBI	-	builds acid from
[Ref.: #65441]	maltitol ChEBI	-	carbon source
[Ref.: #65441]	maltose ChEBI	-	builds acid from
[Ref.: #65441]	melibiose ChEBI	-	builds acid from
[Ref.: #65441]	mesaconate ChEBI	-	carbon source
[Ref.: #65441]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from
[Ref.: #65441]	myo-inositol ChEBI	-	builds acid from
[Ref.: #65441]	myo-inositol ChEBI	-	carbon source
[Ref.: #65441]	N-acetylgalactosamine ChEBI	-	carbon source
[Ref.: #65441]	N-acetylglucosamine ChEBI	-	carbon source
[Ref.: #65441]	phenylacetate ChEBI	-	carbon source
[Ref.: #65441]	propionate ChEBI	-	carbon source
[Ref.: #65441]	putrescine ChEBI	-	carbon source
[Ref.: #65441]	pyruvate ChEBI	-	carbon source
[Ref.: #65441]	raffinose ChEBI	-	builds acid from
[Ref.: #65441]	ribitol ChEBI	-	builds acid from
[Ref.: #65441]	ribitol ChEBI	-	carbon source
[Ref.: #65441]	salicin ChEBI	-	builds acid from
[Ref.: #65441]	salicin ChEBI	-	carbon source
[Ref.: #65441]	sodium acetate ChEBI	-	carbon source
[Ref.: #65441]	sodium citrate ChEBI	-	carbon source
[Ref.: #65441]	starch ChEBI	-	hydrolysis
[Ref.: #65441]	suberic acid ChEBI	-	carbon source
[Ref.: #65441]	sucrose ChEBI	-	builds acid from
[Ref.: #65441]	sucrose ChEBI	-	carbon source
[Ref.: #65441]	trans-aconitate ChEBI	-	carbon source
[Ref.: #65441]	trehalose ChEBI	-	builds acid from
[Ref.: #65441]	tyrosine ChEBI	-	hydrolysis
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #65441]		hydrogen sulfide ChEBI	no
[Ref.: #65441]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #65441]	beta-galactosidase	-	3.2.1.23
[Ref.: #65441]	catalase	+	1.11.1.6
[Ref.: #65441]	cytochrome oxidase	+	1.9.3.1
[Ref.: #65441]	lysine decarboxylase	-	4.1.1.18
[Ref.: #65441]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #65441]	urease	-	3.5.1.5

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #65441]

Fatty acid	Percentage
C15:0 anteiso	46.8
C15:0 iso	17.9
C16:0 iso	15.7
C16:0	7.1
C17:0 anteiso	7.1
C17:0 iso	3.4
C14:0	3

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #65071]	Sample type/isolated from	metalworking fluid (coolant lubricant)
[Ref.: #65071]	Geographic location (country and/or sea, region)	Hessen, Gießen
[Ref.: #65071]	Country	Germany
[Ref.: #65071]	Country ISO 3 Code	DEU
[Ref.: #65071]	Continent	Europe

[Ref.: #65441]	Sample type/isolated from	a coolant lubricant
[Ref.: #65441]	Country	Germany
[Ref.: #65441]	Country ISO 3 Code	DEU
[Ref.: #65441]	Continent	Europe
[Ref.: #65441]	Enrichment culture	nutrient agar
[Ref.: #65441]	Enrichment culture temperature	30 ̊C
[Ref.: #65441]	Isolation/enrichment procedure	the strain was initially isolated on nutrient agar (NA; Sigma-Aldrich) at 30C and also further maintained and subcultivated on this agar at 30C for 48 h

[Ref.: #120658]	Sample type/isolated from	Environment, Metalworking fluid
[Ref.: #120658]	Geographic location (country and/or sea, region)	Giessen, Hessen
[Ref.: #120658]	Country	Germany
[Ref.: #120658]	Country ISO 3 Code	DEU
[Ref.: #120658]	Continent	Europe
[Ref.: #120658]	Isolation date	2016-12-30
* marker position based on {}

Isolation sources categories

#Engineered	#Industrial	#Industrial production
#Environmental	#Aquatic	-

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

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Biosafety level

Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #65071]	Marinicrinis lubricantis strain KSS164-79 16S ribosomal RNA gene, partial sequence	MG589643	1463	ENA	2086470 tax ID

[Ref.: #65071]	GC-content	62.8 mol%
[Ref.: #65441]	GC-content	62.8 mol%	high performance liquid chromatography (HPLC)

Availability in culture collections External links

[Ref.: #65071]

Culture collection no.

DSM 104943, CCM 8749, CIP 111345, LMG 30062

[Ref.: #111199]

SI-ID 404345

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Marinicrinis lubricantis sp. nov., isolated from a coolant lubricant.	Kampfer P, Busse HJ, Glaeser SP	Int J Syst Evol Microbiol	10.1099/ijsem.0.002603	2018	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#65071

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 104943

#65441

Peter Kämpfer, Hans-Jürgen Busse, Stefanie P. Glaeser: Marinicrinis lubricantis sp. nov., isolated from a coolant lubricant. IJSEM 68: 1018 - 1022 2018 ( DOI 10.1099/ijsem.0.002603 , PubMed 29458483 )

#111199

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID404345.1 )

#120658 Collection of Institut Pasteur ; Curators of the CIP; CIP 111345
* These data were automatically processed and therefore are not curated

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