Strain identifier
BacDive ID: 158306
Type strain: 
Species: Monoglobus pectinilyticus
Strain Designation: 14
Strain history: D. Rosendale; Plant & Food Res., New Zealand; 14.
NCBI tax ID(s): 1981510 (species)
version 8.1 (current version)
General
@ref: 64968
BacDive-ID: 158306
DSM-Number: 104782
keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic, Gram-positive, coccus-shaped
description: Monoglobus pectinilyticus 14 is an anaerobe, mesophilic, Gram-positive bacterium that was isolated from fresh human faecal sample of a healthy 27-year-old woman.
NCBI tax id
- NCBI tax id: 1981510
- Matching level: species
strain history
| @ref | history |
|---|---|
| 64968 | <- C. Kim, Plant & Food Research, Palmerston North, New Zealand; 14 |
| 67770 | D. Rosendale; Plant & Food Res., New Zealand; 14. |
doi: 10.13145/bacdive158306.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Clostridia
- order: Eubacteriales
- family: Oscillospiraceae
- genus: Monoglobus
- species: Monoglobus pectinilyticus
- full scientific name: Monoglobus pectinilyticus Kim et al. 2017
@ref: 64968
domain: Bacteria
phylum: Firmicutes
class: Clostridia
order: Eubacteriales
family: Oscillospiraceae
genus: Monoglobus
species: Monoglobus pectinilyticus
full scientific name: Monoglobus pectinilyticus Kim et al. 2017
strain designation: 14
type strain: yes
Morphology
cell morphology
| @ref | gram stain | cell length | cell shape | motility | confidence |
|---|---|---|---|---|---|
| 65227 | positive | 0.6-0.7 µm | coccus-shaped | no | |
| 69480 | positive | 100 |
Culture and growth conditions
culture medium
- @ref: 64968
- name: PYG MEDIUM (MODIFIED) (DSMZ Medium 104)
- growth: yes
- link: https://mediadive.dsmz.de/medium/104
- composition: Name: PYG MEDIUM (modified) (DSMZ Medium 104; with strain-specific modifications) Composition: Yeast extract 10.0 g/l Fructose 5.0 g/l Trypticase peptone 5.0 g/l Peptone 5.0 g/l Beef extract 5.0 g/l Glucose 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l K2HPO4 0.04 g/l KH2PO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Hemin 0.005 g/l Ethanol 0.0038 g/l Resazurin 0.001 g/l Vitamin K1 NaOH Tween 80 Distilled water
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 64968 | positive | growth | 37 | mesophilic |
| 65227 | positive | growth | 25-40 | |
| 65227 | positive | optimum | 30-40 | |
| 67770 | positive | growth | 37 | mesophilic |
culture pH
| @ref | ability | type | pH | PH range |
|---|---|---|---|---|
| 65227 | positive | growth | 6-8.5 | alkaliphile |
| 65227 | positive | optimum | 6.5-8 |
Physiology and metabolism
oxygen tolerance
| @ref | oxygen tolerance | confidence |
|---|---|---|
| 65227 | anaerobe | |
| 69480 | anaerobe | 99.999 |
spore formation
| @ref | type of spore | spore formation | confidence |
|---|---|---|---|
| 65227 | endospore | no | |
| 69480 | no | 99.671 | |
| 69481 | no | 97 |
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 65227 | 22590 | arabinan | - | growth |
| 65227 | 22599 | arabinose | + | fermentation |
| 65227 | 22599 | arabinose | + | growth |
| 65227 | 28427 | arabinoxylan | - | growth |
| 65227 | 28793 | beta-D-glucan | - | growth |
| 65227 | casein | - | hydrolysis | |
| 65227 | 17057 | cellobiose | - | growth |
| 65227 | 62968 | cellulose | - | growth |
| 65227 | 18024 | D-galacturonic acid | + | fermentation |
| 65227 | 18024 | D-galacturonic acid | + | growth |
| 65227 | 4853 | esculin | - | hydrolysis |
| 65227 | 28757 | fructose | + | fermentation |
| 65227 | 28757 | fructose | + | growth |
| 65227 | 37165 | galactan | - | growth |
| 65227 | 16813 | galactitol | - | growth |
| 65227 | 28260 | galactose | - | growth |
| 65227 | 5291 | gelatin | - | hydrolysis |
| 65227 | 17754 | glycerol | - | growth |
| 65227 | 18287 | L-fucose | - | growth |
| 65227 | 17716 | lactose | - | growth |
| 65227 | 17306 | maltose | - | growth |
| 65227 | 37684 | mannose | - | growth |
| 65227 | 17309 | pectin | + | fermentation |
| 65227 | 17309 | pectin | + | growth |
| 65227 | 16634 | raffinose | - | growth |
| 65227 | 26546 | rhamnose | - | growth |
| 65227 | 17814 | salicin | - | growth |
| 65227 | 30911 | sorbitol | - | growth |
| 65227 | 28017 | starch | - | growth |
| 65227 | 17992 | sucrose | - | growth |
| 65227 | 37166 | xylan | - | growth |
| 65227 | 18222 | xylose | + | fermentation |
| 65227 | 18222 | xylose | + | growth |
metabolite production
| @ref | Chebi-ID | metabolite | production |
|---|---|---|---|
| 65227 | 30089 | acetate | yes |
| 65227 | 16526 | carbon dioxide | yes |
| 65227 | 18276 | dihydrogen | yes |
| 65227 | 15740 | formate | yes |
| 65227 | 35581 | indole | no |
| 65227 | 24996 | lactate | yes |
enzymes
| @ref | value | activity | ec |
|---|---|---|---|
| 65227 | cytochrome oxidase | - | 1.9.3.1 |
| 65227 | catalase | + | 1.11.1.6 |
| 65227 | urease | - | 3.5.1.5 |
| 65227 | lipase | - | |
| 65227 | lecithinase | - |
Isolation, sampling and environmental information
isolation
| @ref | sample type | geographic location | country | origin.country | continent | latitude | longitude | enrichment culture | enrichment culture composition | enrichment culture duration | enrichment culture temperature | isolation procedure |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 64968 | fresh human faecal sample of a healthy 27-year-old woman | Palmerston North (40.3523° S, 175.6082° E) | New Zealand | NZL | Australia and Oceania | -40.3523 | 175.608 | |||||
| 65227 | faeces from a healthy 27-year-old female living in New Zealand who had not been prescribed antibiotics for 6 months prior to the sample collection | The New Zealand Institute for Plant and Food Research Limited, Palmerston North | New Zealand | NZL | Australia and Oceania | basal medium (10 mL) | (per litre) 2 g Difco peptone water; 2 g Bacto yeast extract; 0.5 g bile salts (Oxoid); 10 ml Mineral A stock solution (0.4 g K2HPO4 dissolved in 100 ml distilled water); 10 ml Mineral B stock solution (0.4 g KH2PO4 ; 0.1 g MgSO4x7H2O; 0.1 g CaCl2x6H2O; and 1.0 g NaCl dissolved in 100 ml distilled water); 2 ml Tween 80 (Fischer Scientific); 0.05 g haemin (Sigma); 10 µl vitamin K (Sigma); 0.5 g L-cysteine-HCl; 0.02 g resazurin; and 4.2 g NaHCO3 . The basal medium was prepared under O2 -free 100 % | 15 days | 37 | Fresh stool was weighed and 5 g of stool was mixed with anaerobically prepared phosphate buffered saline (PBS) solution to make a 10 % faecal slurry. The faecal slurry was serially diluted (10-fold) until 10^9-fold dilution was achieved, and 0.5 ml of 10^-7, 10^-8, and 10^-9 dilutions were used to inoculate the basal medium (10 ml per tube) containing 0.2 % (w/v) kiwifruit pectin. After 15 days of incubation at 37°C with constant rotary shaking, a small volume of culture was spread over the surf | ||
| 67770 | Healthy 27-yearold female | New Zealand | NZL | Australia and Oceania |
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Host Body Product | #Gastrointestinal tract | #Feces (Stool) |
| #Host | #Human | #Female |
taxonmaps
- @ref: 69479
- File name: preview.99_36206.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_1614;96_17312;97_21291;98_26698;99_36206&stattab=map
- Last taxonomy: Monoglobus pectinilyticus subclade
- 16S sequence: KY978733
- Sequence Identity:
- Total samples: 60384
- soil counts: 218
- aquatic counts: 1942
- animal counts: 58083
- plant counts: 141
Safety information
risk assessment
- @ref: 64968
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 64968
- description: Monoglobus pectinilyticus strain 14 16S ribosomal RNA gene, partial sequence
- accession: KY978733
- length: 1493
- database: ena
- NCBI tax ID: 1981510
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Monoglobus pectinilyticus 14 | GCA_002874775 | chromosome | ncbi | 1981510 |
| 66792 | Monoglobus pectinilyticus strain 14 | 1981510.4 | complete | patric | 1981510 |
| 66792 | Monoglobus pectinilyticus 14 | 2833582041 | complete | img | 1981510 |
GC content
| @ref | GC-content | method |
|---|---|---|
| 64968 | 37.21 | |
| 65227 | 37.21 | genome sequence analysis |
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| spore-forming | no | 97 | no |
| motile | no | 93.88 | yes |
| gram-positive | yes | 92.653 | yes |
| anaerobic | yes | 99.515 | yes |
| aerobic | no | 98.848 | yes |
| halophile | no | 87.872 | no |
| spore-forming | no | 81.343 | no |
| glucose-util | yes | 77.979 | no |
| flagellated | no | 98.368 | yes |
| thermophile | no | 91.973 | yes |
| glucose-ferment | yes | 76.126 | no |
External links
@ref: 64968
culture collection no.: DSM 104782, JCM 31914
straininfo link
- @ref: 111120
- straininfo: 398739
literature
- topic: Phylogeny
- Pubmed-ID: 29039307
- title: Monoglobus pectinilyticus gen. nov., sp. nov., a pectinolytic bacterium isolated from human faeces.
- authors: Kim CC, Kelly WJ, Patchett ML, Tannock GW, Jordens Z, Stoklosinski HM, Taylor JW, Sims IM, Bell TJ, Rosendale DI
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijsem.0.002395
- year: 2017
- mesh: Adult, Bacterial Typing Techniques, Base Composition, Clostridiales/*classification/genetics/isolation & purification, DNA, Bacterial/genetics, Feces/*microbiology, Female, Humans, New Zealand, Pectins/*metabolism, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA
- topic2: Pathogenicity
Reference
| @id | authors | title | doi/url | catalogue | journal | pubmed |
|---|---|---|---|---|---|---|
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |||
| 64968 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-104782 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 104782) | |||
| 65227 | Caroline C. Kim, William J. Kelly, Mark L. Patchett, Gerald W. Tannock, Z. Jordens, Halina M. Stoklosinski, Jordan W. Taylor, Ian M. Sims, Tracey J. Bell, Douglas I. Rosendale | Monoglobus pectinilyticus gen. nov., sp. nov., a pectinolytic bacterium isolated from human faeces | 10.1099/ijsem.0.002395 | IJSEM 67: 4992-4998 2017 | 29039307 | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |||
| 66794 | Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg | BRENDA, the ELIXIR core data resource in 2021: new developments and updates | 10.1093/nar/gkaa1025 | Nucleic Acids Res. 49: D498-D508 2020 | 33211880 | |
| 67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | MicrobeAtlas 1.0 beta | https://microbeatlas.org/ | |||
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |||
| 69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | Predictions based on the model GenomeNet Sporulation v. 1 | 10.21203/rs.3.rs-2527258/v1 | |||
| 111120 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID398739.1 |