Strain identifier

BacDive ID: 158278

Type strain: Yes

Species: Neisseria dumasiana

Strain Designation: 93087

Strain history: <- W. Wolfgang, Wadsworth Center, NYS Dept. of Health, Albany, USA; 93087

NCBI tax ID(s): 1931275 (species)

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General

@ref: 64938

BacDive-ID: 158278

DSM-Number: 104677

keywords: genome sequence, 16S sequence, Bacteria, facultative anaerobe, mesophilic, Gram-negative, coccus-shaped, colony-forming, human pathogen

description: Neisseria dumasiana 93087 is a facultative anaerobe, mesophilic, Gram-negative human pathogen that forms circular colonies and was isolated from human sputum of a 79-year-old female.

NCBI tax id

  • NCBI tax id: 1931275
  • Matching level: species

strain history

  • @ref: 64938
  • history: <- W. Wolfgang, Wadsworth Center, NYS Dept. of Health, Albany, USA; 93087

doi: 10.13145/bacdive158278.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Betaproteobacteria
  • order: Neisseriales
  • family: Neisseriaceae
  • genus: Neisseria
  • species: Neisseria dumasiana
  • full scientific name: Neisseria dumasiana Wroblewski et al. 2017

@ref: 64938

domain: Bacteria

phylum: Proteobacteria

class: Betaproteobacteria

order: Neisseriales

family: Neisseriaceae

genus: Neisseria

species: Neisseria dumasiana

full scientific name: Neisseria dumasiana Wroblewski et al. 2017

strain designation: 93087

type strain: yes

Morphology

cell morphology

  • @ref: 65274
  • gram stain: negative
  • cell length: 0.8 µm
  • cell shape: coccus-shaped
  • motility: no

colony morphology

  • @ref: 65274
  • type of hemolysis: gamma
  • colony size: 1.9-2.8 mm
  • colony color: grey
  • colony shape: circular
  • incubation period: 2 days
  • medium used: chocolate agar

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
64938COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
64938BHI MEDIUM (DSMZ Medium 215)yeshttps://mediadive.dsmz.de/medium/215Name: BHI MEDIUM (DSMZ Medium 215) Composition: Brain heart infusion 37.0 g/l Distilled water
65274chocolate agaryessupplemented with 10% horse blood
65274Brain Heart infusion brothyes
65274MacConkey agarno
64938TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
64938positivegrowth37mesophilic
65274positivegrowth22-42
65274positiveoptimum37mesophilic
65274nogrowth4psychrophilic

culture pH

@refabilitytypepHPH range
65274positivegrowth6-9.5alkaliphile
65274positiveoptimum7.2

Physiology and metabolism

oxygen tolerance

  • @ref: 65274
  • oxygen tolerance: facultative anaerobe

halophily

@refsaltgrowthtested relationconcentration
65274NaClpositivegrowth2 %
65274NaClnogrowth5 %
65274NaClnogrowth>5 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6527415824D-fructose-builds acid from
6527417634D-glucose-builds acid from
6527416899D-mannitol-builds acid from
6527465327D-xylose-builds acid from
652744853esculin-hydrolysis
652745291gelatin-hydrolysis
6527417306maltose-builds acid from
6527417632nitrate+reduction
6527417992sucrose-builds acid from
6527417992sucrose+builds acid from
6527416199urea-hydrolysis
6837717634D-glucose+builds acid from
6837717306maltose-builds acid from
6837717992sucrose-builds acid from
6837718257ornithine-degradation
6837716199urea-hydrolysis
6837727897tryptophan-energy source

metabolite production

@refChebi-IDmetaboliteproduction
6527435581indoleno
6837735581indoleno

metabolite tests

  • @ref: 68377
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
65274catalase+1.11.1.6
65274cytochrome oxidase+1.9.3.1
65274proline-arylamidase+3.4.11.5
65274lipase-
65274alkaline phosphatase-3.1.3.1
65274gamma-glutamyltransferase-2.3.2.2
65274ornithine decarboxylase-4.1.1.17
65274urease-3.5.1.5
65274beta-galactosidase-3.2.1.23
68377tryptophan deaminase-4.1.99.1
68377proline-arylamidase+3.4.11.5
68377beta-galactosidase-3.2.1.23
68377alkaline phosphatase-3.1.3.1
68377lipase-
68377urease-3.5.1.5
68377ornithine decarboxylase-4.1.1.17
68377beta-lactamase-3.5.2.6

API NH

@refPENGLUFRUMALSACODCURELIPPALbeta GALProAGGTIND
64938-++/--------++-
64938-+--------++/--

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitude
64938human sputum of a 79-year-old femaleNew York State, Saratoga CountyUSAUSANorth America43.11-73.87
65274sputum of a 79-year old female and from a dog´s mouthNew York StateUSAUSANorth America

isolation source categories

Cat1Cat2Cat3
#Host#Mammals#Canidae (Dog)
#Host Body Product#Fluids#Sputum

taxonmaps

  • @ref: 69479
  • File name: preview.99_5447.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15355;96_837;97_979;98_4070;99_5447&stattab=map
  • Last taxonomy: Neisseria dumasiana subclade
  • 16S sequence: KY417828
  • Sequence Identity:
  • Total samples: 2986
  • soil counts: 48
  • aquatic counts: 153
  • animal counts: 2784
  • plant counts: 1

Safety information

risk assessment

  • @ref: 64938
  • pathogenicity human: yes, in single cases
  • pathogenicity animal: yes, in single cases
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 64938
  • description: Neisseria dumasiana strain 93087 16S ribosomal RNA gene, partial sequence
  • accession: KY417828
  • length: 1463
  • database: ena
  • NCBI tax ID: 1931275

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Neisseria dumasiana 93087GCA_002108505contigncbi1931275
66792Neisseria dumasiana LMG 30012GCA_022870885completencbi1931275

GC content

@refGC-contentmethod
6493850.5
6527450.5genome sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
flagellatedno93.568no
gram-positiveno98.7yes
anaerobicno97.133yes
aerobicno89.753no
halophileno76.604no
spore-formingno96.743no
motileno87.765no
glucose-fermentno87.616no
thermophileno99.321yes
glucose-utilyes77.506no

External links

@ref: 64938

culture collection no.: DSM 104677, LMG 30012

straininfo link

  • @ref: 111096
  • straininfo: 403323

literature

  • topic: Phylogeny
  • Pubmed-ID: 28933320
  • title: Neisseria dumasiana sp. nov. from human sputum and a dog's mouth.
  • authors: Wroblewski D, Cole J, McGinnis J, Perez M, Wilson H, Mingle LA, Musser KA, Wolfgang WJ
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.002148
  • year: 2017
  • mesh: Animals, Bacterial Typing Techniques, Base Composition, California, DNA, Bacterial/genetics, Dogs/*microbiology, Fatty Acids/chemistry, Humans, Mouth/microbiology, Neisseria/*classification/genetics/isolation & purification, New York, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sputum/*microbiology
  • topic2: Pathogenicity

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
64938Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-104677Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 104677)
65274Danielle Wroblewski, Jocelyn Cole, Jana McGinnis, Maria Perez, Harriet Wilson, Lisa A. Mingle, Kimberlee A. Musser, William J. WolfgangNeisseria dumasiana sp. nov. from human sputum and a dog's mouth10.1099/ijsem.0.002148IJSEM 67: 4304-4310 201728933320
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
68377Automatically annotated from API NH
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
111096Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID403323.1