Strain identifier

BacDive ID: 158014

Type strain: Yes

Species: Actinoplanes sediminis

Strain Designation: M4147

Strain history: <- Q. Xie,Inst. of Tropical Bioscience and Biotechnology, Haikou,China; M4147

NCBI tax ID(s): 1734445 (species)

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General

@ref: 64587

BacDive-ID: 158014

DSM-Number: 100965

keywords: 16S sequence, Bacteria, aerobe, spore-forming, Gram-positive

description: Actinoplanes sediminis M4147 is an aerobe, spore-forming, Gram-positive bacterium that builds substrate hyphae and was isolated from sediment.

NCBI tax id

  • NCBI tax id: 1734445
  • Matching level: species

strain history

  • @ref: 64587
  • history: <- Q. Xie,Inst. of Tropical Bioscience and Biotechnology, Haikou,China; M4147

doi: 10.13145/bacdive158014.20240510.9

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micromonosporales
  • family: Micromonosporaceae
  • genus: Actinoplanes
  • species: Actinoplanes sediminis
  • full scientific name: Actinoplanes sediminis Qu et al. 2018

@ref: 64587

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Micromonosporaceae

genus: Actinoplanes

species: Actinoplanes sediminis

full scientific name: Actinoplanes sediminis Qu et al. 2018

strain designation: M4147

type strain: yes

Morphology

cell morphology

  • @ref: 65514
  • gram stain: positive

colony morphology

  • @ref: 65514
  • colony color: yellowish-orange

multicellular morphology

  • @ref: 65514
  • forms multicellular complex: yes
  • complex name: substrate hyphae

pigmentation

  • @ref: 65514
  • production: yes
  • name: soluble pigment

multimedia

  • @ref: 64587
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_100965.jpg
  • caption: Medium 83 28°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
64587CZAPEK PEPTONE AGAR (DSMZ Medium 83)yeshttps://mediadive.dsmz.de/medium/83Name: CZAPEK PEPTONE AGAR (DSMZ Medium 83) Composition: Sucrose 30.0 g/l Agar 20.0 g/l Peptone 5.0 g/l NaNO3 3.0 g/l Yeast extract 2.0 g/l K2HPO4 1.0 g/l MgSO4 x 7 H2O 0.5 g/l KCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Distilled water
64587TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)yeshttps://mediadive.dsmz.de/medium/535Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
64587GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
65514ISP 2yes
65514ISP 6yes
65514ATCC 172yes
65514M8yes

culture temp

@refgrowthtypetemperature
65514positivegrowth16-37
65514positiveoptimum28
64587positivegrowth28

culture pH

@refabilitytypepHPH range
65514positivegrowth5.5-9.0alkaliphile
65514positiveoptimum7.0

Physiology and metabolism

oxygen tolerance

  • @ref: 65514
  • oxygen tolerance: aerobe

spore formation

  • @ref: 65514
  • spore formation: yes

halophily

  • @ref: 65514
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-4 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6551417057cellobiose+carbon source
6551462968cellulose-hydrolysis
6551415824D-fructose+carbon source
6551412936D-galactose-carbon source
6551417634D-glucose+carbon source
6551416899D-mannitol-carbon source
6551416024D-mannose-carbon source
6551465327D-xylose-carbon source
655144853esculin-hydrolysis
655145291gelatin-hydrolysis
6551415428glycine+nitrogen source
6551417268myo-inositol-carbon source
6551430849L-arabinose+carbon source
6551416467L-arginine+nitrogen source
6551417196L-asparagine+nitrogen source
6551415971L-histidine+nitrogen source
6551418019L-lysine+nitrogen source
6551417295L-phenylalanine-nitrogen source
6551462345L-rhamnose-carbon source
6551417266L-sorbose-carbon source
6551417895L-tyrosine-nitrogen source
6551417716lactose+carbon source
6551417306maltose+carbon source
6551417632nitrate+reduction
6551416634raffinose-carbon source
6551416634raffinose+carbon source
6551417814salicin+carbon source
6551428017starch+hydrolysis
6551417992sucrose+carbon source
6551416199urea+hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
6551416136hydrogen sulfideno
6551489634melaninno

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    65514C17:031.8
    65514C16:0 anteiso16.2
    65514C17:0 anteiso10.9
    65514C16:0 iso8.1
    65514C15:0 anteiso5.9
    65514C16:0 10-methyl5.8
    65514C14:0 9-methyl5.4
    65514C15:0 iso4.7
    65514C15:03.5
    65514C17:1ω10c2
    65514C14:01.9
    65514C17:0 iso1.7
    65514C16:01.1
    65514C16:1ω9c0.9
  • type of FA analysis: whole cell analysis
  • incubation medium: ISP 2
  • incubation temperature: 28
  • incubation time: 14
  • library/peak naming table: ACTIN6
  • system: MIS MIDI
  • method/protocol: Sasser, 1990

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitudeenrichment cultureenrichment culture compositionenrichment culture durationenrichment culture temperature
64587sedimentSyroy, Megas Gialos (37° 25.9' N, 24° 56.3' E)GreeceGRCEurope41.316733.3833
65514sediment sampleMegas Gialos, SyrosGreeceGRCEurope37.431724.9383M4supplemented with potassium dichromate (30 ml/l), novobiocin (5 mg/l) and nystatin (30 mg/l).4 weeks28

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Sediment
#Environmental#Terrestrial#Sediment

Safety information

risk assessment

  • @ref: 64587
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 64587
  • description: Actinoplanes sediminis strain M4I47 16S ribosomal RNA gene, partial sequence
  • accession: KR347172
  • length: 1492
  • database: nuccore
  • NCBI tax ID: 1734445

GC content

  • @ref: 65514
  • GC-content: 71.5
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 64587

culture collection no.: DSM 100965

straininfo link

  • @ref: 110861
  • straininfo: 401569

literature

  • topic: Phylogeny
  • Pubmed-ID: 29125458
  • title: Actinoplanes sediminis sp. nov., isolated from marine sediment.
  • authors: Qu Z, Bao XD, Xie QY, Zhao YX, Yan B, Dai HF, Chen HQ
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.002451
  • year: 2017
  • mesh: Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, *Geologic Sediments, Greece, Micromonosporaceae/*classification/genetics/isolation & purification, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistry
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
64587Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-100965Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 100965)
65514Zhi Qu, Xiao-dong Bao, Qing-yi Xie, You-xing Zhao, Bing Yan, Hao-fu Dai and Hui-Qin ChenActinoplanes sediminis sp. nov., isolated from marine sediment10.1099/ijsem.0.002451IJSEM 68: 71-75 201829125458
110861Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID401569.1