Strain identifier

BacDive ID: 15506

Type strain: Yes

Species: Streptomyces purpureus

Strain Designation: KA-279

Strain history: KCC A-0172 <-- A. Matsumae KA-279.

NCBI tax ID(s): 1951 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 10982

BacDive-ID: 15506

DSM-Number: 43362

keywords: genome sequence, 16S sequence, Bacteria, spore-forming, mesophilic, antibiotic compound production

description: Streptomyces purpureus KA-279 is a spore-forming, mesophilic bacterium that builds an aerial mycelium and produces antibiotic compounds.

NCBI tax id

  • NCBI tax id: 1951
  • Matching level: species

strain history

@refhistory
10982<- NRRL <- A. Matsumai, KA-279
67770KCC A-0172 <-- A. Matsumae KA-279.

doi: 10.13145/bacdive15506.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces purpureus
  • full scientific name: Streptomyces purpureus (Matsumae and Hata 1968) Goodfellow et al. 1986

  • synonyms

    @refsynonym
    20215Kitasatoa nagasakiensis
    20215Kitasatoa diplospora
    20215Kitasatoa purpurea
    20215Kitasatoa kauaiensis

@ref: 10982

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces purpureus

full scientific name: Streptomyces purpureus (Matsumae and Hata 1968) Goodfellow et al. 1986

strain designation: KA-279

type strain: yes

Morphology

colony morphology

@refcolony colorincubation periodmedium used
19570Yellow - Brown10-14 daysISP 2
19570Brown10-14 daysISP 3
19570Brown10-14 daysISP 4
19570Brown10-14 daysISP 5
19570Yellow10-14 daysISP 6
19570Yellow10-14 daysISP 7

multicellular morphology

@refforms multicellular complexcomplex namecomplex colormedium name
19570yesAerial MyceliumWhiteISP 2
19570yesAerial MyceliumGreyISP 3
19570yesAerial MyceliumWhiteISP 4
19570yesAerial MyceliumGreyISP 5
19570yesAerial MyceliumGreyISP 6
19570yesAerial MyceliumGreyISP 7

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
10982GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
19570ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19570ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19570ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19570ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19570ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19570ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
10982OAT FLAKES AGAR WITH YEAST EXTRACT (DSMZ Medium 248)yeshttps://mediadive.dsmz.de/medium/248Name: OAT FLAKES AGAR WITH YEAST EXTRACT (DSMZ Medium 248) Composition: Agar 20.0 g/l Oat flakes 20.0 g/l Yeast extract 2.0 g/l MnCl2 x 4 H2O 0.001 g/l FeSO4 x 7 H2O 0.001 g/l ZnSO4 x 7 H2O 0.001 g/l Distilled water
10982CZAPEK PEPTONE AGAR (DSMZ Medium 83)yeshttps://mediadive.dsmz.de/medium/83Name: CZAPEK PEPTONE AGAR (DSMZ Medium 83) Composition: Sucrose 30.0 g/l Agar 20.0 g/l Peptone 5.0 g/l NaNO3 3.0 g/l Yeast extract 2.0 g/l K2HPO4 1.0 g/l MgSO4 x 7 H2O 0.5 g/l KCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
19570positiveoptimum28mesophilic
10982positivegrowth28mesophilic
67770positivegrowth28mesophilic

Physiology and metabolism

spore formation

  • @ref: 19570
  • spore description: Formation of spore chains (rectiflixibilis), spore surface smooth
  • type of spore: spore
  • spore formation: yes

compound production

@refcompound
10982chloramphenicol
20216Chloramphenicol

halophily

  • @ref: 19570
  • salt: NaCl
  • growth: positive
  • tested relation: maximum
  • concentration: 5 %

observation

  • @ref: 67770
  • observation: quinones: MK-9(H6), MK-9(H8)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1957017234glucose+
1957022599arabinose+
1957017992sucrose-
1957018222xylose+
1957017268myo-inositol+
1957029864mannitol+
1957028757fructose+
1957026546rhamnose+
1957016634raffinose+
683685291gelatin-hydrolysis
6836827897tryptophan-energy source
6836816199urea-hydrolysis
6836816947citrate-assimilation
6836818257ornithine-degradation
6836825094lysine-degradation
6836829016arginine+hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
6777017698chloramphenicolyes
6836815688acetoinno
6836835581indoleno
6836816136hydrogen sulfideno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
6836815688acetoin-
6836835581indole-

enzymes

@refvalueactivityec
68368gelatinase-
68368tryptophan deaminase+4.1.99.1
68368urease-3.5.1.5
68368ornithine decarboxylase-4.1.1.17
68368lysine decarboxylase-4.1.1.18
68368arginine dihydrolase+3.5.3.6
68368beta-galactosidase-3.2.1.23

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGEL
19570-+-----+---

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
10982soil
67770SoilKauai Island, HIUSAUSANorth America

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
109821Risk group (German classification)
195701Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Streptomyces purpureus gene for 16S ribosomal RNA, partial sequence, strain: JCM 3172D43981120ena1951
20218Streptomyces purpureus 16S rRNA gene, type strain LMG 19368AJ7813241476ena1951
20218Streptomyces purpureus gene for 16S rRNA, partial sequence, strain: NBRC 13927AB1845471478ena1951
20218Streptomyces purpureus strain NRRL B-5403 16S ribosomal RNA gene, partial sequenceHQ6508081527ena1951

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Streptomyces purpureus JCM 3172GCA_014648195scaffoldncbi1951
66792Streptomyces purpureus strain JCM 31721951.3wgspatric1951

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
flagellatedno97.523no
gram-positiveyes86.358no
anaerobicno99.266no
aerobicyes91.786no
halophileno94.361no
spore-formingyes93.342no
thermophileno98.07no
glucose-utilyes89.475no
motileno93.844no
glucose-fermentno91.699no

External links

@ref: 10982

culture collection no.: DSM 43362, ATCC 27787, JCM 3172, KCC A-0172, NRRL B-5403, BCRC 12101, CGMCC 4.1225, IFO 13927, IMET 9041, KCTC 9187, NBRC 13927, NCIMB 11311, VKM Ac-1298, VTT E-072761

straininfo link

  • @ref: 84592
  • straininfo: 13366

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
10982Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 43362)https://www.dsmz.de/collection/catalogue/details/culture/DSM-43362
19570Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM43362.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20216Curators of the HKIhttp://www.leibniz-hki.de/de/Collection Description Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. Hans-Knöll-Institut (HKI)
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
84592Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID13366.1StrainInfo: A central database for resolving microbial strain identifiers