Strain identifier

BacDive ID: 1544

Type strain: Yes

Species: Piscibacillus salipiscarius

Strain Designation: RBU1-1

Strain history: CIP <- 2007, JCM <- 2005, S. Tanasupawat, Chulalongkorn Univ., Bangkok, Thailand: strain RBU1-1

NCBI tax ID(s): 1294022 (strain), 299480 (species)

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General

@ref: 15842

BacDive-ID: 1544

DSM-Number: 21622

keywords: genome sequence, 16S sequence, Bacteria, aerobe, spore-forming, mesophilic, Gram-positive, motile, rod-shaped

description: Piscibacillus salipiscarius RBU1-1 is an aerobe, spore-forming, mesophilic bacterium that was isolated from fermented fish .

NCBI tax id

NCBI tax idMatching level
1294022strain
299480species

strain history

@refhistory
15842<- JCM <- S. Tanasupawat; RBU1-1
67770S. Tanasupawat; Chulalongkorn Univ., Thailand; RBU1-1.
122387CIP <- 2007, JCM <- 2005, S. Tanasupawat, Chulalongkorn Univ., Bangkok, Thailand: strain RBU1-1

doi: 10.13145/bacdive1544.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Piscibacillus
  • species: Piscibacillus salipiscarius
  • full scientific name: Piscibacillus salipiscarius Tanasupawat et al. 2007

@ref: 15842

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Bacillaceae

genus: Piscibacillus

species: Piscibacillus salipiscarius

full scientific name: Piscibacillus salipiscarius Tanasupawat et al. 2007

strain designation: RBU1-1

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
32088positive2.75 µm0.45 µmrod-shapedyes
69480positive100
122387positiverod-shapedyes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
15842HALOBACTERIA MEDIUM (DSMZ Medium 372)yeshttps://mediadive.dsmz.de/medium/372Name: HALOBACTERIA MEDIUM (DSMZ Medium 372; with strain-specific modifications) Composition: NaCl 100.0 g/l MgSO4 x 7 H2O 20.0 g/l Agar 20.0 g/l Yeast extract 5.0 g/l Casamino acids 5.0 g/l Na3-citrate 3.0 g/l KCl 2.0 g/l Na glutamate 1.0 g/l FeCl2 x 4 H2O 0.036 g/l MnCl2 x 4 H2O 0.00036 g/l Distilled water
33317MEDIUM 204 - for Halorubrum, Marinococcus and NatrialbayesDistilled water make up to (1000.000 ml);Sodium chloride (200.000 g);Potassium chloride (2.000 g);ManganeseII chloride tetrahydrate (0.360 mg);Magnesium sulphate heptahydrate (20.000 g);Agar (20.000 g);Yeast extract (5.000 g);Ferrous chloride tetrahydrate
122387CIP Medium 649yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=649
122387CIP Medium 204yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=204

culture temp

@refgrowthtypetemperaturerange
15842positivegrowth30mesophilic
32088positivegrowth15-48
32088positiveoptimum37mesophilic
33317positivegrowth37mesophilic
67770positivegrowth37mesophilic

culture pH

@refabilitytypepHPH range
32088positivegrowth5.0-9.0alkaliphile
32088positiveoptimum7

Physiology and metabolism

oxygen tolerance

  • @ref: 32088
  • oxygen tolerance: aerobe

spore formation

@refspore formationconfidence
32088yes
69481yes98
69480yes99.336

halophily

@refsaltgrowthtested relationconcentration
32088NaClpositivegrowth2.0-30 %
32088NaClpositiveoptimum15 %

observation

  • @ref: 67770
  • observation: quinones: MK-7

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3208828757fructose+carbon source
3208828260galactose+carbon source
3208817234glucose+carbon source
3208817754glycerol+carbon source
3208833942ribose+carbon source
3208817992sucrose+carbon source
12238717632nitrate-reduction
12238716301nitrite-reduction

metabolite production

  • @ref: 122387
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
32088catalase+1.11.1.6
32088gelatinase+
32088cytochrome oxidase+1.9.3.1
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase+3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase-
68382acid phosphatase-3.1.3.2
68382alpha-chymotrypsin+3.4.21.1
68382trypsin+3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
122387oxidase+
122387catalase+1.11.1.6
122387urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
122387-+++-+--++----++----

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
15842fermented fish (pla-ra)ThailandTHAAsia
67770Fermented fish (pla-ra) in Thailand
122387Animal, Fish, fermentedThailandTHAAsia

isolation source categories

Cat1Cat2Cat3
#Engineered#Food production#Seafood
#Host#Fishes
#Engineered#Food production#Fermented

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
158421Risk group (German classification)
1223871Risk group (French classification)

Sequence information

16S sequences

  • @ref: 15842
  • description: Piscibacillus salipiscarius gene for 16S ribosomal RNA, partial sequence
  • accession: AB194046
  • length: 1520
  • database: ena
  • NCBI tax ID: 1294022

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Piscibacillus salipiscarius JCM 131881294022.5wgspatric1294022
66792Piscibacillus salipiscarius JCM 131882675903365draftimg1294022
67770Piscibacillus salipiscarius JCM 13188GCA_001311865contigncbi1294022

GC content

@refGC-contentmethod
1584236.7high performance liquid chromatography (HPLC)
3208836.7

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes98no
motileyes88.593no
gram-positiveyes87.244no
anaerobicno98.32yes
aerobicyes96.27no
halophileyes94.045yes
spore-formingyes92yes
glucose-utilyes86.503yes
flagellatedyes56.292no
thermophileno92.873yes
glucose-fermentno89.695no

External links

@ref: 15842

culture collection no.: DSM 21622, JCM 13188, PCU 270, TISTR 1571, CIP 109653

straininfo link

  • @ref: 71189
  • straininfo: 309475

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny17625167Piscibacillus salipiscarius gen. nov., sp. nov., a moderately halophilic bacterium from fermented fish (pla-ra) in Thailand.Tanasupawat S, Namwong S, Kudo T, Itoh TInt J Syst Evol Microbiol10.1099/ijs.0.64945-02007Aerobiosis, Animals, Bacillaceae/chemistry/*classification/*isolation & purification/physiology, Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Diaminopimelic Acid/analysis, Fatty Acids/analysis, Fishes/*microbiology, *Food Microbiology, Genes, rRNA, Hydrogen-Ion Concentration, Locomotion, Microscopy, Electron, Scanning, Molecular Sequence Data, Peptidoglycan/chemistry, Phospholipids/analysis, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sequence Homology, Nucleic Acid, Sodium Chloride/metabolism, Spores, Bacterial, Temperature, ThailandBiotechnology
Phylogeny19643892Piscibacillus halophilus sp. nov., a moderately halophilic bacterium from a hypersaline Iranian lake.Amoozegar MA, Sanchez-Porro C, Rohban R, Hajighasemi M, Ventosa AInt J Syst Evol Microbiol10.1099/ijs.0.012013-02009Bacillaceae/*classification/genetics/*isolation & purification/metabolism, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fatty Acids/chemistry/metabolism, Fresh Water/*microbiology, Iran, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Sodium Chloride/*metabolismMetabolism

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
15842Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 21622)https://www.dsmz.de/collection/catalogue/details/culture/DSM-21622
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
32088Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2833428776041
33317Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/7420
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
71189Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID309475.1StrainInfo: A central database for resolving microbial strain identifiers
122387Curators of the CIPCollection of Institut Pasteur (CIP 109653)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20109653