Name: Piscibacillus salipiscarius RBU1-1
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 1544

Type strain:

Species: Piscibacillus salipiscarius

Strain Designation: RBU1-1

Culture col. no.: DSM 21622, JCM 13188, PCU 270, TISTR 1571, CIP 109653

Strain history: CIP <- 2007, JCM <- 2005, S. Tanasupawat, Chulalongkorn Univ., Bangkok, Thailand: strain RBU1-1

NCBI tax ID(s): 1294022 (strain), 299480 (species)

SI-ID 309475

JCM 13188, TISTR 1571, CIP 109653

Section

Piscibacillus salipiscarius RBU1-1 is an aerobe, spore-forming, mesophilic bacterium that was isolated from fermented fish .

Gram-positive
motile
rod-shaped
spore-forming
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Piscibacillus*
	Species	**Piscibacillus salipiscarius**
	Full Scientific Name (LPSN)	Piscibacillus salipiscarius Tanasupawat et al. 2007

Information on morphological and physiological properties Morphology

[Ref.: #32088]	Gram stain	positive
[Ref.: #122387]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #32088]	Cell length	2.75 µm

[Ref.: #32088]	Cell width	0.45 µm

[Ref.: #32088]	Cell shape	rod-shaped
[Ref.: #122387]	Cell shape	rod-shaped

[Ref.: #32088]	Motility	yes
[Ref.: #122387]	Motility	yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #15842]

Culture medium

HALOBACTERIA MEDIUM (DSMZ Medium 372)

[Ref.: #15842]

Culture medium growth

[Ref.: #15842]

Culture medium link

Medium recipe at MediaDive

[Ref.: #15842]

Culture medium composition

expand / minimize

[Ref.: #33317]

Culture medium

MEDIUM 204 - for Halorubrum, Marinococcus and Natrialba

[Ref.: #33317]

Culture medium growth

[Ref.: #33317]

Culture medium composition

expand / minimize

[Ref.: #122387]

Culture medium

CIP Medium 649

[Ref.: #122387]

Culture medium growth

[Ref.: #122387]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #122387]

Culture medium

CIP Medium 204

[Ref.: #122387]

Culture medium growth

[Ref.: #122387]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #15842]	growth	30 °C
[Ref.: #32088]	growth	15-48 °C
[Ref.: #32088]	optimum	37 °C
[Ref.: #33317]	growth	37 °C
[Ref.: #67770]	growth	37 °C

[Ref.: #15842]	Temperature range	mesophilic
[Ref.: #32088]	Temperature range	mesophilic
[Ref.: #33317]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #32088]			optimum	7
[Ref.: #32088]			growth	5.0-9.0

Information on physiology and metabolism Physiology and metabolism

[Ref.: #32088]

Oxygen tolerance

aerobe

[Ref.: #32088]	Ability of spore formation	yes
[Ref.: #69481]	Ability of spore formation	yes Confidence in %98
[Ref.: #69480]	Ability of spore formation	yes Confidence in %99.336

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #32088]		NaCl		growth	2.0-30	%
[Ref.: #32088]		NaCl		optimum	15	%

[Ref.: #67770]

Observation

quinones: MK-7

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #32088]	fructose ChEBI	+	carbon source
[Ref.: #32088]	galactose ChEBI	+	carbon source
[Ref.: #32088]	glucose ChEBI	+	carbon source
[Ref.: #32088]	glycerol ChEBI	+	carbon source
[Ref.: #122387]	nitrate ChEBI	-	reduction
[Ref.: #122387]	nitrite ChEBI	-	reduction
[Ref.: #32088]	ribose ChEBI	+	carbon source
[Ref.: #32088]	sucrose ChEBI	+	carbon source

	Metabolite production	Metabolite	Production
[Ref.: #122387]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	-	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	+	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	+	3.2.1.31	* from API zym
[Ref.: #32088]	catalase	+	1.11.1.6
[Ref.: #122387]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #32088]	cytochrome oxidase	+	1.9.3.1
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #32088]	gelatinase	+
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	-		* from API zym
[Ref.: #122387]	oxidase	+
[Ref.: #68382]	trypsin	+	3.4.21.4	* from API zym
[Ref.: #122387]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #122387]	12033	-	+	+	+	-	+	-	-	+	+	-	-	-	-	+	+	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #15842]	Sample type/isolated from	fermented fish (pla-ra)
[Ref.: #15842]	Country	Thailand
[Ref.: #15842]	Country ISO 3 Code	THA
[Ref.: #15842]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Fermented fish (pla-ra) in Thailand

[Ref.: #122387]	Sample type/isolated from	Animal, Fish, fermented
[Ref.: #122387]	Country	Thailand
[Ref.: #122387]	Country ISO 3 Code	THA
[Ref.: #122387]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Engineered	#Food production	#Seafood
#Host	#Fishes	-
#Engineered	#Food production	#Fermented

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #15842]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #122387]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #15842]	Piscibacillus salipiscarius gene for 16S ribosomal RNA, partial sequence	AB194046	1520	ENA	1294022 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Piscibacillus salipiscarius JCM 13188	GCA_001311865	contig	GenBank	1294022 tax ID
[Ref.: #66792]	Piscibacillus salipiscarius JCM 13188	1294022.5	wgs	PATRIC	1294022 tax ID	*
[Ref.: #66792]	Piscibacillus salipiscarius JCM 13188	2675903365	draft	JGI IMG/M	1294022 tax ID	*

[Ref.: #15842]	GC-content	36.7 mol%	high performance liquid chromatography (HPLC)
[Ref.: #32088]	GC-content	36.7 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Piscibacillus salipiscarius JCM 13188 PATRIC: 1294022.5
[Ref.: #69481]	spore-forming	yes	98	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Piscibacillus salipiscarius JCM 13188 PATRIC: 1294022.5
[Ref.: #69480]	motile	yes	88.593	no
[Ref.: #69480]	gram-positive	yes	87.244	no
[Ref.: #69480]	anaerobic	no	98.32	yes
[Ref.: #69480]	aerobic	yes	96.27	no
[Ref.: #69480]	halophile	yes	94.045	yes
[Ref.: #69480]	spore-forming	yes	92	yes
[Ref.: #69480]	glucose-util	yes	86.503	yes
[Ref.: #69480]	flagellated	yes	56.292	no
[Ref.: #69480]	thermophile	no	92.873	yes
[Ref.: #69480]	glucose-ferment	no	89.695	no

Availability in culture collections External links

[Ref.: #15842]

Culture collection no.

DSM 21622, JCM 13188, PCU 270, TISTR 1571, CIP 109653

[Ref.: #71189]

SI-ID 309475

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Piscibacillus halophilus sp. nov., a moderately halophilic bacterium from a hypersaline Iranian lake.	Amoozegar MA, Sanchez-Porro C, Rohban R, Hajighasemi M, Ventosa A	Int J Syst Evol Microbiol	10.1099/ijs.0.012013-0	2009	*
Phylogeny	Piscibacillus salipiscarius gen. nov., sp. nov., a moderately halophilic bacterium from fermented fish (pla-ra) in Thailand.	Tanasupawat S, Namwong S, Kudo T, Itoh T	Int J Syst Evol Microbiol	10.1099/ijs.0.64945-0	2007	*

References

#15842

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 21622

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#32088

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #28334 (see below)

#33317 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68382

Automatically annotated from API zym .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#71189

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID309475.1 )

#122387 Collection of Institut Pasteur ; Curators of the CIP; CIP 109653

#28334

IJSEM 1413 2007 ( DOI 10.1099/ijs.0.64945-0 , PubMed 17625167 )

* These data were automatically processed and therefore are not curated

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