Strain identifier

BacDive ID: 15411

Type strain: Yes

Species: Streptomyces minutiscleroticus

Strain history: KCC A-0102 <-- RIA 885 <-- HACC 147.

NCBI tax ID(s): 68238 (species)

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General

@ref: 9443

BacDive-ID: 15411

DSM-Number: 40301

keywords: genome sequence, 16S sequence, Bacteria, spore-forming, mesophilic, Gram-positive, antibiotic compound production

description: Streptomyces minutiscleroticus DSM 40301 is a spore-forming, mesophilic, Gram-positive bacterium that produces antibiotic compounds and was isolated from Soil.

NCBI tax id

  • NCBI tax id: 68238
  • Matching level: species

strain history

@refhistory
9443<- E.B. Shirling, ISP <- M.J. Thirumalachar, HACC
67770KCC A-0102 <-- RIA 885 <-- HACC 147.

doi: 10.13145/bacdive15411.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces minutiscleroticus
  • full scientific name: Streptomyces minutiscleroticus (Thirumalachar 1965) Pridham 1970 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Chainia minutisclerotica

@ref: 9443

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces minutiscleroticus

full scientific name: Streptomyces minutiscleroticus (Thirumalachar 1965) Pridham 1970 emend. Lanoot et al. 2005

type strain: yes

Morphology

cell morphology

  • @ref: 69480
  • gram stain: positive
  • confidence: 100

multimedia

  • @ref: 9443
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_40301.jpg
  • caption: Medium 65 28°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
9443GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
9443STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium252.pdf

culture temp

@refgrowthtypetemperaturerange
18580positiveoptimum30mesophilic
9443positivegrowth28mesophilic
67770positivegrowth28mesophilic

Physiology and metabolism

spore formation

@refspore formationconfidence
69481yes100
69480yes100

compound production

@refcompound
9443aburamycin
9443antibiotic M5-18903

halophily

  • @ref: 18580
  • salt: NaCl
  • growth: positive
  • tested relation: maximum
  • concentration: 2.5 %

observation

  • @ref: 67770
  • observation: quinones: MK-9(H8)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1858017234glucose+
1858022599arabinose+
1858017992sucrose-
1858018222xylose-
1858017268myo-inositol-
1858029864mannitol+
1858028757fructose-
1858026546rhamnose+
1858016634raffinose-
1858062968cellulose-
683685291gelatin+hydrolysis
6836827897tryptophan-energy source
6836816199urea-hydrolysis
6836816947citrate-assimilation
6836818257ornithine-degradation
6836825094lysine-degradation
6836829016arginine-hydrolysis

metabolite production

@refmetaboliteproductionChebi-ID
67770aburamycin Ayes
68368acetoinyes15688
68368indoleno35581
68368hydrogen sulfideno16136

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
6836815688acetoin+
6836835581indole-

enzymes

@refvalueactivityec
68368gelatinase+
68368tryptophan deaminase-4.1.99.1
68368urease-3.5.1.5
68368ornithine decarboxylase-4.1.1.17
68368lysine decarboxylase-4.1.1.18
68368arginine dihydrolase-3.5.3.6
68368beta-galactosidase+3.2.1.23

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGEL
18580+--------++

Isolation, sampling and environmental information

isolation

  • @ref: 67770
  • sample type: Soil

taxonmaps

  • @ref: 69479
  • File name: preview.99_3171.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15;96_91;97_97;98_106;99_3171&stattab=map
  • Last taxonomy: Streptomyces
  • 16S sequence: EF178696
  • Sequence Identity:
  • Total samples: 28
  • soil counts: 12
  • animal counts: 16

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
185801German classification
94431Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Streptomyces minutiscleroticus gene for 16S ribosomal RNA, partial sequence, strain: JCM 4790D44365121ena68238
20218Streptomyces minutiscleroticus gene for 16S rRNA, partial sequence, strain: NBRC 13000AB1842491476ena68238
20218Streptomyces minutiscleroticus gene for 16S rRNA, partial sequence, strain: NBRC 13361AB1843511475ena68238
20218Streptomyces minutiscleroticus strain NRRL B-12202 16S ribosomal RNA gene, partial sequenceEF1786961480ena68238

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Streptomyces minutiscleroticus JCM 4790GCA_014650875scaffoldncbi68238
66792Streptomyces minutiscleroticus strain JCM 479068238.12wgspatric68238

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
flagellatedno96.964no
gram-positiveyes89.546no
anaerobicno98.771no
aerobicyes93.546no
halophileno94.286no
spore-formingyes97.327no
thermophileno98.019yes
glucose-utilyes89.572yes
motileno93.462no
glucose-fermentno88.482no

External links

@ref: 9443

culture collection no.: DSM 40301, ATCC 17757, CBS 662.72, HACC 147, IFO 13361, ISP 5301, NBRC 13361, RIA 1322, RIA 885, JCM 3102, ATCC 19346, BCRC 12544, CBS 231.65, CGMCC 4.1981, CMI 112786, IFO 13000, JCM 4790, KCTC 9123, LMG 20062, NBRC 13000, NCIMB 10996, NRRL B-12202, PCM 2304

straininfo link

  • @ref: 84500
  • straininfo: 36721

literature

  • topic: Metabolism
  • Pubmed-ID: 7333971
  • title: Microbial conversion of ansamitocin.
  • authors: Nakahama K, Izawa M, Asai M, Kida M, Kishi T
  • journal: J Antibiot (Tokyo)
  • DOI: 10.7164/antibiotics.34.1581
  • year: 1981
  • mesh: Bacteria/*metabolism, Dealkylation, Fungi/*metabolism, Maytansine/analogs & derivatives/*metabolism, Oxazines/*metabolism, Streptomyces/metabolism, Yeasts/metabolism

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
9443Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 40301)https://www.dsmz.de/collection/catalogue/details/culture/DSM-40301
18580Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM40301.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
84500Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID36721.1StrainInfo: A central database for resolving microbial strain identifiers