Strain identifier

BacDive ID: 15393

Type strain: Yes

Species: Streptomyces mashuensis

Strain Designation: 449, NNRL B-3352, NNRL B-8164

Strain history: CIP <- 2003, JCM <- 1983, KCC <- IFO <- SAJ <- ISP <- S. Suzuki: strain 449

NCBI tax ID(s): 33904 (species)

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General

@ref: 9278

BacDive-ID: 15393

DSM-Number: 40221

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, spore-forming, antibiotic compound production

description: Streptomyces mashuensis 449 is an obligate aerobe, spore-forming bacterium that builds an aerial mycelium and produces antibiotic compounds.

NCBI tax id

  • NCBI tax id: 33904
  • Matching level: species

strain history

@refhistory
9278<- E.B. Shirling, ISP <- S. Suzuki, IPCR 449
67770KCC S-0059 <-- T. Sawazaki 449.
120845CIP <- 2003, JCM <- 1983, KCC <- IFO <- SAJ <- ISP <- S. Suzuki: strain 449

doi: 10.13145/bacdive15393.20240916.9.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces mashuensis
  • full scientific name: Streptomyces mashuensis (Sawazaki et al. 1955) Witt and Stackebrandt 1991

  • synonyms

    @refsynonym
    20215Streptoverticillium kishiwadense
    20215Streptoverticillium mashuense
    20215Streptomyces kishiwadensis
    20215Streptomyces mashuensis

@ref: 9278

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces mashuensis

full scientific name: Streptomyces mashuensis (Sawazaki et al. 1955) Witt and Stackebrandt 1991

strain designation: 449, NNRL B-3352, NNRL B-8164

type strain: yes

Morphology

cell morphology

@refgram staincell shapemotilityconfidence
120845positivefilament-shapedno
69480positive90.236

colony morphology

@refcolony colorincubation periodmedium usedhemolysis ability
19424Brown10-14 daysISP 2
19424Yellow10-14 daysISP 3
19424Yellow10-14 daysISP 4
1942410-14 daysISP 5
19424Brown10-14 daysISP 6
19424Brown10-14 daysISP 7
1208451

multicellular morphology

@refforms multicellular complexcomplex namecomplex colormedium name
19424yesAerial MyceliumRoseISP 2
19424yesAerial MyceliumBrown redISP 3
19424yesAerial MyceliumBrown redISP 4
19424yesAerial MyceliumBrown redISP 5
19424noISP 6
19424yesAerial MyceliumBrown redISP 7

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
9278GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
19424ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19424ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19424ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19424ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19424ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19424ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
37415MEDIUM 57 - for Streptomyces, Nocardioides, Lentzea albidocapillata and Streptoverticillium reticulumyesDistilled water make up to (1000.000 ml);Agar (15.000 g);Glucose (4.000g);Yeast extract (4.000 g);Malt extract (10.000 g);Calcium carbonate (2.000 g)
9278ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium84.pdf
120845CIP Medium 57yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=57

culture temp

@refgrowthtypetemperature
9278positivegrowth28
37415positivegrowth30
67770positivegrowth28
120845positivegrowth25-45
120845nogrowth10

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
120845obligate aerobe
69480aerobe90.988

spore formation

@refspore descriptionspore formationconfidence
19424Formation of spore chains: verticillus, spore surface: smoothyes
69480yes93.412
69481yes100

compound production

@refcompound
9278streptomycin
9278anti-fungal polyene antibiotic
20216Streptomycin
20216Antifungal antibiotic agent

halophily

@refsaltgrowthtested relationconcentration
120845NaClpositivegrowth0-2 %
120845NaClnogrowth4 %
120845NaClnogrowth6 %
120845NaClnogrowth8 %
120845NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1942462968cellulose-
1942416634raffinose-
1942426546rhamnose-
1942428757fructose+
1942429864mannitol-
1942417268myo-inositol+
1942418222xylose-
1942417992sucrose+
1942422599arabinose-
1942417234glucose+
683685291gelatin-hydrolysis
6836827897tryptophan-energy source
6836816199urea-hydrolysis
6836816947citrate-assimilation
6836818257ornithine-degradation
6836825094lysine-degradation
6836829016arginine-hydrolysis
12084516947citrate-carbon source
1208454853esculin-hydrolysis
120845606565hippurate+hydrolysis
12084517632nitrate-reduction
12084516301nitrite-reduction
12084517632nitrate-respiration

metabolite production

@refChebi-IDmetaboliteproduction
6777017076streptomycinyes
6836815688acetoinyes
6836835581indoleno
6836816136hydrogen sulfideno
12084535581indoleno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole testmethylred-test
6836815688acetoin+
6836835581indole-
12084515688acetoin+
12084517234glucose-

enzymes

@refvalueactivityec
68368gelatinase-
68368tryptophan deaminase-4.1.99.1
68368urease-3.5.1.5
68368ornithine decarboxylase-4.1.1.17
68368lysine decarboxylase-4.1.1.18
68368arginine dihydrolase-3.5.3.6
68368beta-galactosidase-3.2.1.23
120845oxidase-
120845beta-galactosidase-3.2.1.23
120845alcohol dehydrogenase-1.1.1.1
120845gelatinase+
120845amylase+
120845DNase+
120845caseinase+3.4.21.50
120845catalase+1.11.1.6
120845tween esterase-
120845gamma-glutamyltransferase+2.3.2.2
120845lecithinase+
120845lipase+
120845lysine decarboxylase-4.1.1.18
120845ornithine decarboxylase-4.1.1.17
120845phenylalanine ammonia-lyase-4.3.1.24
120845protease-
120845tryptophan deaminase-
120845urease+3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)-
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase+3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
120845-++--+++--++--------

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGEL
19424---------+-

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
120845++-+---+-+---------+-----------+---------------------------++------------+--------------+-++-+--+--

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentgeographic location
9278soilJapanJPNAsia
67770SoilJapanJPNAsiaLake Mashu, Hokkaido
120845Environment, SoilJapanJPNAsiaMashu lake, Hokkaido

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
92781Risk group (German classification)
194241Risk group (German classification)
1208451Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Streptomyces mashuensis 16S ribosomal RNA gene, partial sequenceU72173270nuccore33904
20218Streptomyces mashuensis strain DSM 40221 16S ribosomal RNA gene, partial sequenceFJ4061181362nuccore33904
20218Streptomyces mashuensis strain DSM 40221 16S ribosomal RNA gene, partial sequenceGU383221782nuccore33904
20218Streptomyces mashuensis 16S rRNA gene, strain DSM40221X793231518nuccore33904
20218Streptomyces mashuensis gene for 16S ribosomal RNA, partial sequence, strain: JCM 4059D43993121nuccore33904
20218Streptomyces mashuensis gene for 16S rRNA, partial sequence, strain: NBRC 12888AB1848521461nuccore33904
20218Streptomyces mashuensis strain NRRL B-8164T 16S ribosomal RNA gene, partial sequenceDQ4425261489nuccore33904

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Streptomyces mashuensis JCM 4059GCA_014654785contigncbi33904
66792Streptomyces mashuensis strain JCM 405933904.3wgspatric33904

Genome-based predictions

predictions

@reftraitmodeldescriptionpredictionconfidencetraining_data
69481spore-formingspore-formingAbility to form endo- or exosporesyes100no
69480gram-positivegram-positivePositive reaction to Gram-stainingyes90.236no
69480anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)no96.334no
69480spore-formingspore-formingAbility to form endo- or exosporesyes93.412no
69480aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)yes90.988no
69480thermophilicthermophileAbility to grow at temperatures above or equal to 45°Cno96.5yes
69480flagellatedmotile2+Ability to perform flagellated movementno87.5no

External links

@ref: 9278

culture collection no.: DSM 40221, ATCC 23934, CBS 918.68, IFO 12888, ISP 5221, JCM 4059, JCM 4650, NBRC 12888, RIA 1165, BCRC 12420, CBS 279.65, CGMCC 4.2007, CIP 108143, IFM 1082, IMET 42941, LMG 8603, NCB 258, NRRL B-3352, NRRL B-8164, VKM Ac-949, CCRC 12420, KCC S-0650

straininfo link

  • @ref: 84483
  • straininfo: 389332

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
9278Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 40221)https://www.dsmz.de/collection/catalogue/details/culture/DSM-40221
19424Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM40221.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20216Curators of the HKIhttp://www.leibniz-hki.de/de/Collection Description Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. Hans-Knöll-Institut (HKI)
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
37415Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/5733
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
84483Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID389332.1StrainInfo: A central database for resolving microbial strain identifiers
120845Curators of the CIPCollection of Institut Pasteur (CIP 108143)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20108143