Strain identifier

BacDive ID: 153

Type strain: No

Species: Actinomyces naeslundii

Strain Designation: Bowden X600S-96, CS 1752, X-600

Strain history: CIP <- 1982, ATCC <- L. Georg, CDC: strain X-600 <- A. Howell: strain CS 1752

NCBI tax ID(s): 1655 (species)

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General

@ref: 6829

BacDive-ID: 153

DSM-Number: 17233

keywords: 16S sequence, Bacteria, microaerophile, mesophilic, Gram-positive, rod-shaped, human pathogen

description: Actinomyces naeslundii Bowden X600S-96 is a microaerophile, mesophilic, Gram-positive human pathogen that was isolated from human dental plaque.

NCBI tax id

  • NCBI tax id: 1655
  • Matching level: species

strain history

@refhistory
6829<- CCUG; <- G. Bowden; <- M. A. Gerencser; WVU 447 <- L. K. Georg; CDC X-600 <- A. Howell; CS 1752
67770N. Maeda TPR-9 <-- ATCC 19039 <-- L. Georg CDC X-600 <-- A. Howell CS1752.
121220CIP <- 1982, ATCC <- L. Georg, CDC: strain X-600 <- A. Howell: strain CS 1752

doi: 10.13145/bacdive153.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Actinomycetales
  • family: Actinomycetaceae
  • genus: Actinomyces
  • species: Actinomyces naeslundii
  • full scientific name: Actinomyces naeslundii corrig. Thompson and Lovestedt 1951 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Actinomyces naeslundi

@ref: 6829

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Actinomycetaceae

genus: Actinomyces

species: Actinomyces naeslundii

full scientific name: Actinomyces naeslundii Thompson and Lovestedt 1951 emend. Henssge et al. 2009 emend. Nouioui et al. 2018

strain designation: Bowden X600S-96, CS 1752, X-600

type strain: no

Morphology

cell morphology

  • @ref: 121220
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 121220
  • hemolysis ability: 1

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
6829COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium693.pdf
37076MEDIUM 6 - Columbia agar with 10 % horse bloodyesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml)
121220CIP Medium 6yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=6

culture temp

@refgrowthtypetemperaturerange
37076positivegrowth37mesophilic
6829positivegrowth37mesophilic
67770positivegrowth37mesophilic
121220positivegrowth25-41
121220nogrowth10psychrophilic
121220nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
6829microaerophile
121220facultative anaerobe

halophily

@refsaltgrowthtested relationconcentration
121220NaClpositivegrowth0-2 %
121220NaClnogrowth4 %
121220NaClnogrowth6 %
121220NaClnogrowth8 %
121220NaClnogrowth10 %

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose+builds acid from16634
68371melezitose-builds acid from6731
68371sucrose+builds acid from17992
68371melibiose+builds acid from28053
68371lactose-builds acid from17716
68371maltose+builds acid from17306
68371salicin+builds acid from17814
68371esculin+builds acid from4853
68371arbutin+builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol+builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol+builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose+builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol+builds acid from17754
121220citrate-carbon source16947
121220esculin+hydrolysis4853
121220hippurate-hydrolysis606565
121220nitrate+reduction17632
121220nitrite-reduction16301
121220nitrate+respiration17632
68379nitrate+reduction17632
68379esculin+hydrolysis4853
68379urea-hydrolysis16199
68379gelatin-hydrolysis5291
68379D-glucose+fermentation17634
68379D-xylose-fermentation65327
68379D-mannitol-fermentation16899
68379maltose+fermentation17306
68379lactose-fermentation17716
68379sucrose+fermentation17992
68379glycogen-fermentation28087
68380urea-hydrolysis16199
68380arginine-hydrolysis29016
68380D-mannose+fermentation16024
68380raffinose+fermentation16634
68380L-glutamate-degradation29985
68380nitrate+reduction17632
68380tryptophan-energy source27897
68381arginine-hydrolysis29016
68381D-ribose-builds acid from16988
68381D-mannitol-builds acid from16899
68381sorbitol-builds acid from30911
68381lactose-builds acid from17716
68381trehalose+builds acid from27082
68381raffinose+builds acid from16634
68381sucrose+builds acid from17992
68381L-arabinose-builds acid from30849
68381D-arabitol-builds acid from18333
68381alpha-cyclodextrin-builds acid from40585
68381hippurate-hydrolysis606565
68381glycogen-builds acid from28087
68381pullulan-builds acid from27941
68381maltose+builds acid from17306
68381melibiose+builds acid from28053
68381melezitose-builds acid from6731
68381methyl beta-D-glucopyranoside-builds acid from320055
68381D-tagatose-builds acid from16443
68381urea-hydrolysis16199

antibiotic resistance

  • @ref: 121220
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: yes
  • is resistant: no

metabolite production

@refChebi-IDmetaboliteproduction
12122035581indoleno
6838035581indoleno
6838115688acetoinno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole testmethylred-test
6838115688acetoin-
6838035581indole-
12122015688acetoin-
12122017234glucose+

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase+3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)+
68382alkaline phosphatase-3.1.3.1
68381urease-3.5.1.5
68381beta-mannosidase-3.2.1.25
68381glycyl tryptophan arylamidase-
68381N-acetyl-beta-glucosaminidase-3.2.1.52
68381pyrrolidonyl arylamidase-3.4.19.3
68381beta-galactosidase+3.2.1.23
68381Alanyl-Phenylalanyl-Proline arylamidase-
68381alkaline phosphatase-3.1.3.1
68381alpha-galactosidase+3.2.1.22
68381beta-glucuronidase-3.2.1.31
68381beta-glucosidase+3.2.1.21
68381arginine dihydrolase-3.5.3.6
68380serine arylamidase-
68380glutamyl-glutamate arylamidase-
68380histidine arylamidase-
68380glycin arylamidase-
68380alanine arylamidase-3.4.11.2
68380tyrosine arylamidase+
68380pyrrolidonyl arylamidase-3.4.19.3
68380leucine arylamidase+3.4.11.1
68380phenylalanine arylamidase+
68380leucyl glycin arylamidase-3.4.11.1
68380proline-arylamidase+3.4.11.5
68380L-arginine arylamidase-
68380alkaline phosphatase-3.1.3.1
68380tryptophan deaminase-4.1.99.1
68380alpha-fucosidase-3.2.1.51
68380glutamate decarboxylase-4.1.1.15
68380N-acetyl-beta-glucosaminidase-3.2.1.52
68380beta-glucuronidase-3.2.1.31
68380alpha-arabinosidase-3.2.1.55
68380beta-glucosidase+3.2.1.21
68380alpha-glucosidase+3.2.1.20
68380beta-Galactosidase 6-phosphate-
68380beta-galactosidase+3.2.1.23
68380alpha-galactosidase+3.2.1.22
68380arginine dihydrolase-3.5.3.6
68380urease-3.5.1.5
68379catalase-1.11.1.6
68379gelatinase-
68379urease-3.5.1.5
68379beta-glucosidase+3.2.1.21
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379beta-galactosidase+3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379pyrrolidonyl arylamidase-3.4.19.3
68379pyrazinamidase+3.5.1.B15
121220oxidase-
121220beta-galactosidase+3.2.1.23
121220alcohol dehydrogenase-1.1.1.1
121220gelatinase-
121220amylase+
121220DNase-
121220caseinase-3.4.21.50
121220catalase-1.11.1.6
121220tween esterase-
121220gamma-glutamyltransferase-2.3.2.2
121220lecithinase-
121220lipase-
121220lysine decarboxylase-4.1.1.18
121220ornithine decarboxylase-4.1.1.17
121220phenylalanine ammonia-lyase-4.3.1.24
121220protease-
121220tryptophan deaminase-
121220urease+3.5.1.5

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELControlGLURIBXYLMANMALLACSACGLYGCAT
6829++---+--+---++/---+-+--
52676++-+-++-+---++--+-+--

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
121220--+--++---++++-++---

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
121220+---+/-----++++---+-+--+-++++/-+-+++/-+/--+----+/-------+/---

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
6829--++-++---++--+---+-++-+-----

API rID32STR

@refADH Argbeta GLUbeta GARbeta GURalpha GALPALRIBMANSORLACTRERAFSACLARADARLCDEXVPAPPAbeta GALPyrAbeta NAGGTAHIPGLYGPULMALMELMLZMbeta DGTAGbeta MANURE
52676-+--+-----+++-----+------++-----

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
6829human dental plaqueUSAUSANorth America
52676Human dental plaque [6551]
67770Plaque from human tooth
121220Human, Dental plaque

isolation source categories

Cat1Cat2Cat3
#Host#Human
#Host Body-Site#Oral cavity and airways#Plaque
#Host Body Product#Oral cavity and Airways#Dental plaque

taxonmaps

  • @ref: 69479
  • File name: preview.99_5620.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_124;96_361;97_2224;98_2795;99_5620&stattab=map
  • Last taxonomy: Actinomyces naeslundii subclade
  • 16S sequence: LC037216
  • Sequence Identity:
  • Total samples: 23134
  • soil counts: 345
  • aquatic counts: 595
  • animal counts: 22069
  • plant counts: 125

Safety information

risk assessment

@refpathogenicity humanpathogenicity animalbiosafety levelbiosafety level comment
6829yesyes2Risk group (German classification)
1212202Risk group (French classification)

Sequence information

16S sequences

  • @ref: 67770
  • description: Actinomyces naeslundii gene for 16S ribosomal RNA, partial sequence, strain: JCM 8350
  • accession: LC037216
  • length: 1522
  • database: ena
  • NCBI tax ID: 1655

External links

@ref: 6829

culture collection no.: DSM 17233, ATCC 19039, CCUG 35337, DSM 43325, WVU 447, CDC X-600, JCM 8350, CIP 104837, CIP 82.9, CIP 100654

straininfo link

  • @ref: 69835
  • straininfo: 36817

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Pathogenicity18312575Interactions between salivary Bifidobacterium adolescentis and other oral bacteria: in vitro coaggregation and coadhesion assays.Nagaoka S, Hojo K, Murata S, Mori T, Ohshima T, Maeda NFEMS Microbiol Lett10.1111/j.1574-6968.2008.01092.x2008Bacteria/drug effects/genetics/isolation & purification, *Bacterial Adhesion/drug effects, *Bacterial Physiological Phenomena/drug effects, Bifidobacterium/drug effects/genetics/isolation & purification/*physiology, Biofilms/drug effects/growth & development, Endopeptidase K/pharmacology, Fusobacterium nucleatum/drug effects/genetics/physiology, Hot Temperature, Humans, In Situ Hybridization, Fluorescence, Mouth/*microbiology, Saliva/*microbiologyEnzymology
Pathogenicity24603299Systematic screening of plant extracts from the Brazilian Pantanal with antimicrobial activity against bacteria with cariogenic relevance.Brighenti FL, Salvador MJ, Delbem AC, Delbem AC, Oliveira MA, Soares CP, Freitas LS, Koga-Ito CYCaries Res10.1159/0003572252014Actinomyces/drug effects, Annonaceae/chemistry, Anti-Bacterial Agents/*pharmacology, Arecaceae/chemistry, Brazil, Combretaceae/chemistry, Croton/chemistry, Dental Caries/*microbiology, Gram-Positive Bacteria/*drug effects, Humans, Jatropha/chemistry, Lactobacillus acidophilus/drug effects, Malpighiaceae/chemistry, Melastomataceae/chemistry, Microbial Sensitivity Tests, Phenols/analysis, Phytotherapy/*methods, Plant Extracts/chemistry/*pharmacology, Senna Extract/chemistry, Solvents/chemistry, Streptococcus gordonii/drug effects, Streptococcus mitis/drug effects, Streptococcus mutans/drug effects, Streptococcus sanguis/drug effects, Streptococcus sobrinus/drug effects, Temperature, Terpenes/analysis
29515530Antibacterial Effect of Copaifera duckei Dwyer Oleoresin and Its Main Diterpenes against Oral Pathogens and Their Cytotoxic Effect.Abrao F, Alves JA, Andrade G, de Oliveira PF, Ambrosio SR, Veneziani RCS, Tavares DC, Bastos JK, Martins CHGFront Microbiol10.3389/fmicb.2018.002012018

Reference

@idauthorscataloguedoi/urltitle
6829Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 17233)https://www.dsmz.de/collection/catalogue/details/culture/DSM-17233
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
37076Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/12142
52676Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 35337)https://www.ccug.se/strain?id=35337
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68379Automatically annotated from API Coryne
68380Automatically annotated from API rID32A
68381Automatically annotated from API rID32STR
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69835Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID36817.1StrainInfo: A central database for resolving microbial strain identifiers
121220Curators of the CIPCollection of Institut Pasteur (CIP 100654)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20100654