Strain identifier
BacDive ID: 15274
Type strain: 
Species: Peterkaempfera griseoplana
Strain Designation: AA-223
Strain history: KCC S-0300 <-- CUB 138 <-- ISP 5009 <-- H. D. Tresner AA-223.
NCBI tax ID(s): 66896 (species)
version 8.1 (current version)
General
@ref: 9211
BacDive-ID: 15274
DSM-Number: 40009
keywords: genome sequence, 16S sequence, Bacteria, spore-forming, mesophilic
description: Peterkaempfera griseoplana AA-223 is a spore-forming, mesophilic bacterium that builds an aerial mycelium and was isolated from grassland soil.
NCBI tax id
- NCBI tax id: 66896
- Matching level: species
strain history
| @ref | history |
|---|---|
| 9211 | <- E.B. Shirling, ISP <- H. Tresner, AA-223 |
| 67770 | KCC S-0300 <-- CUB 138 <-- ISP 5009 <-- H. D. Tresner AA-223. |
doi: 10.13145/bacdive15274.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Kitasatosporales
- family: Streptomycetaceae
- genus: Peterkaempfera
- species: Peterkaempfera griseoplana
- full scientific name: Peterkaempfera griseoplana (Backus et al. 1957) Madhaiyan et al. 2022
synonyms
@ref synonym 20215 Streptomyces griseoplanus 20215 Streptacidiphilus griseoplanus
@ref: 9211
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Streptomycetaceae
genus: Streptacidiphilus
species: Streptacidiphilus griseoplanus
full scientific name: Streptacidiphilus griseoplanus (Backus et al. 1957) Nouioui et al. 2019
strain designation: AA-223
type strain: yes
Morphology
cell morphology
| @ref | motility | confidence | gram stain |
|---|---|---|---|
| 69480 | no | 90.396 | |
| 69480 | 100 | positive |
colony morphology
| @ref | colony color | incubation period | medium used |
|---|---|---|---|
| 19384 | Pastel yellow | 10-14 days | ISP 2 |
| 19384 | 10-14 days | ISP 3 | |
| 19384 | 10-14 days | ISP 4 | |
| 19384 | Creme | 10-14 days | ISP 5 |
| 19384 | 10-14 days | ISP 6 | |
| 19384 | Deep orange | 10-14 days | ISP 7 |
multicellular morphology
| @ref | forms multicellular complex | complex name | complex color | medium name |
|---|---|---|---|---|
| 19384 | yes | Aerial Mycelium | Creme | ISP 2 |
| 19384 | yes | Aerial Mycelium | Dusty grey | ISP 3 |
| 19384 | yes | Aerial Mycelium | Dusty grey | ISP 4 |
| 19384 | yes | Aerial Mycelium | Creme | ISP 5 |
| 19384 | no | ISP 6 | ||
| 19384 | yes | Aerial Mycelium | Creme | ISP 7 |
Culture and growth conditions
culture medium
| @ref | name | growth | link | composition |
|---|---|---|---|---|
| 9211 | ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84) | yes | https://mediadive.dsmz.de/medium/84 | Name: ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84) Composition: Agar 20.0 g/l Rolled oats 20.0 g/l ZnSO4 x 7 H2O 0.001 g/l MnCl2 x 4 H2O 0.001 g/l FeSO4 x 7 H2O 0.001 g/l Distilled water |
| 19384 | ISP 2 | yes | Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes | |
| 19384 | ISP 3 | yes | Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms: | |
| 19384 | ISP 4 | yes | Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml | |
| 19384 | ISP 5 | yes | Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes | |
| 19384 | ISP 6 | yes | Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes | |
| 19384 | ISP 7 | yes | Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes | |
| 9211 | GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) | yes | https://mediadive.dsmz.de/medium/65 | Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water |
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 19384 | positive | optimum | 28 | mesophilic |
| 9211 | positive | growth | 28 | mesophilic |
| 67770 | positive | growth | 28 | mesophilic |
Physiology and metabolism
spore formation
| @ref | spore formation | confidence |
|---|---|---|
| 69481 | yes | 100 |
| 69480 | yes | 100 |
compound production
- @ref: 9211
- compound: alazopeptin
halophily
- @ref: 19384
- salt: NaCl
- growth: positive
- tested relation: growth
- concentration: 0 %
observation
- @ref: 67770
- observation: quinones: MK9(H6)
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 68368 | 5291 | gelatin | + | hydrolysis |
| 68368 | 27897 | tryptophan | - | energy source |
| 68368 | 16199 | urea | + | hydrolysis |
| 68368 | 16947 | citrate | - | assimilation |
| 68368 | 18257 | ornithine | - | degradation |
| 68368 | 25094 | lysine | - | degradation |
| 68368 | 29016 | arginine | - | hydrolysis |
metabolite production
| @ref | metabolite | production | Chebi-ID |
|---|---|---|---|
| 67770 | alazopeptin | yes | |
| 68368 | acetoin | yes | 15688 |
| 68368 | indole | no | 35581 |
| 68368 | hydrogen sulfide | no | 16136 |
metabolite tests
| @ref | Chebi-ID | metabolite | voges-proskauer-test | indole test |
|---|---|---|---|---|
| 68368 | 15688 | acetoin | + | |
| 68368 | 35581 | indole | - |
enzymes
| @ref | value | activity | ec |
|---|---|---|---|
| 68368 | gelatinase | + | |
| 68368 | tryptophan deaminase | - | 4.1.99.1 |
| 68368 | urease | + | 3.5.1.5 |
| 68368 | ornithine decarboxylase | - | 4.1.1.17 |
| 68368 | lysine decarboxylase | - | 4.1.1.18 |
| 68368 | arginine dihydrolase | - | 3.5.3.6 |
| 68368 | beta-galactosidase | + | 3.2.1.23 |
| 68382 | acid phosphatase | + | 3.1.3.2 |
| 68382 | naphthol-AS-BI-phosphohydrolase | + | |
| 68382 | alpha-galactosidase | + | 3.2.1.22 |
| 68382 | beta-galactosidase | + | 3.2.1.23 |
| 68382 | beta-glucuronidase | - | 3.2.1.31 |
| 68382 | alpha-glucosidase | + | 3.2.1.20 |
| 68382 | beta-glucosidase | - | 3.2.1.21 |
| 68382 | N-acetyl-beta-glucosaminidase | + | 3.2.1.52 |
| 68382 | alpha-mannosidase | + | 3.2.1.24 |
| 68382 | alpha-fucosidase | - | 3.2.1.51 |
API zym
| @ref | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
|---|---|---|---|---|---|---|---|---|---|---|
| 19384 | + | + | + | + | - | + | - | + | + | - |
API 20E
| @ref | ONPG | ADH Arg | LDC Lys | ODC | CIT | H2S | URE | TDA Trp | IND | VP | GEL |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 19384 | + | - | - | - | - | - | + | - | - | + | + |
Isolation, sampling and environmental information
isolation
| @ref | sample type | geographic location | country | origin.country | continent |
|---|---|---|---|---|---|
| 9211 | grassland soil | Iowa | USA | USA | North America |
| 67770 | Grassland soil near Williamsberg | IA | USA | USA | North America |
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Environmental | #Terrestrial | #Grassland |
| #Environmental | #Terrestrial | #Soil |
Safety information
risk assessment
| @ref | biosafety level | biosafety level comment |
|---|---|---|
| 9211 | 1 | Risk group (German classification) |
| 19384 | 1 | Risk group (German classification) |
Sequence information
16S sequences
| @ref | description | accession | length | database | NCBI tax ID |
|---|---|---|---|---|---|
| 20218 | Streptomyces pratensis strain AS 4.1868 16S ribosomal RNA gene, partial sequence | AY999894 | 1401 | ena | 1169025 |
| 20218 | Streptomyces pratensis strain CGMCC 4.1868 clone 1 16S ribosomal RNA gene, complete sequence | JQ824033 | 1477 | ena | 1169025 |
| 20218 | Streptomyces pratensis strain CGMCC 4.1868 clone 2 16S ribosomal RNA gene, complete sequence | JQ824034 | 1477 | ena | 1169025 |
| 20218 | Streptomyces pratensis strain CGMCC 4.1868 clone 3 16S ribosomal RNA gene, complete sequence | JQ824035 | 1478 | ena | 1169025 |
| 20218 | Streptomyces pratensis strain CGMCC 4.1868 clone 4 16S ribosomal RNA gene, complete sequence | JQ824036 | 1477 | ena | 1169025 |
| 20218 | Streptomyces griseoplanus gene for 16S rRNA, partial sequence | AB122751 | 564 | ena | 66896 |
| 20218 | Streptomyces griseoplanus strain NRRL_ISP 5009 clone 1 16S ribosomal RNA gene, partial sequence | JQ806225 | 1483 | ena | 66896 |
| 20218 | Streptomyces griseoplanus strain NRRL_ISP 5009 clone 2 16S ribosomal RNA gene, partial sequence | JQ806226 | 1480 | ena | 66896 |
| 20218 | Streptomyces griseoplanus strain NRRL_ISP 5009 clone 3 16S ribosomal RNA gene, partial sequence | JQ806227 | 1480 | ena | 66896 |
| 20218 | Streptomyces griseoplanus strain NRRL_ISP 5009 clone 4 16S ribosomal RNA gene, partial sequence | JQ806228 | 1480 | ena | 66896 |
| 20218 | Streptomyces griseoplanus gene for 16S ribosomal RNA, partial sequence, strain: JCM 4300 | D44048 | 120 | ena | 66896 |
| 20218 | Streptomyces griseoplanus gene for 16S rRNA, partial sequence, strain: NBRC 12779 | AB184138 | 1467 | ena | 66896 |
| 20218 | Streptomyces griseoplanus strain NRRL B-3064 clone 1 16S ribosomal RNA gene, partial sequence | JQ806222 | 1480 | ena | 66896 |
| 20218 | Streptomyces griseoplanus strain NRRL B-3064 clone 3 16S ribosomal RNA gene, partial sequence | JQ806223 | 1480 | ena | 66896 |
| 20218 | Streptomyces griseoplanus strain NRRL B-3064 clone 4 16S ribosomal RNA gene, partial sequence | JQ806224 | 1480 | ena | 66896 |
Genome sequences
- @ref: 67770
- description: Peterkaempfera griseoplana NRRL B-3064
- accession: GCA_001418575
- assembly level: scaffold
- database: ncbi
- NCBI tax ID: 66896
GC content
- @ref: 67770
- GC-content: 72.5
- method: genome sequence analysis
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| spore-forming | yes | 100 | no |
| flagellated | no | 97.904 | no |
| gram-positive | yes | 90.294 | no |
| anaerobic | no | 99.095 | no |
| aerobic | yes | 89.435 | no |
| halophile | no | 94.013 | yes |
| spore-forming | yes | 95.523 | no |
| glucose-util | yes | 91.051 | no |
| thermophile | no | 98.654 | no |
| motile | no | 95.226 | no |
| glucose-ferment | no | 88.526 | no |
External links
@ref: 9211
culture collection no.: DSM 40009, AS 4.1868, ATCC 19766, CBS 504.68, IFO 12779, ISP 5009, NBRC 12779, RIA 1046, JCM 4300, BCRC 13649, CGMCC 4.1868, CGMCC 4.1903, JCM 4582, NCIMB 9811, NRRL B-3064, VKM Ac-1727, VTT E-073013
straininfo link
- @ref: 84369
- straininfo: 37095
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 23769815 | Classification of Streptomyces phylogroup pratensis (Doroghazi and Buckley, 2010) based on genetic and phenotypic evidence, and proposal of Streptomyces pratensis sp. nov. | Rong X, Doroghazi JR, Cheng K, Zhang L, Buckley DH, Huang Y | Syst Appl Microbiol | 10.1016/j.syapm.2013.03.010 | 2013 | Bacterial Proteins/genetics, Bacterial Typing Techniques, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Environmental Microbiology, Genes, Essential, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Streptomyces/*classification/genetics/isolation & purification/physiology | Genetics |
| Phylogeny | 27902296 | Allostreptomyces psammosilenae gen. nov., sp. nov., an endophytic actinobacterium isolated from the roots of Psammosilene tunicoides and emended description of the family Streptomycetaceae [Waksman and Henrici (1943)AL] emend. Rainey et al. 1997, emend. Kim et al. 2003, emend. Zhi et al. 2009. | Huang MJ, Rao MPN, Salam N, Xiao M, Huang HQ, Li WJ | Int J Syst Evol Microbiol | 10.1099/ijsem.0.001617 | 2017 | Bacterial Typing Techniques, Base Composition, Caryophyllaceae/*microbiology, China, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Phospholipids/chemistry, *Phylogeny, Plant Roots/*microbiology, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Streptomycetaceae/*classification/genetics/isolation & purification, Vitamin K 2/analogs & derivatives/chemistry | Transcriptome |
| Metabolism | 29230935 | Optimization and characterization of biosurfactant from Streptomyces griseoplanus NRRL-ISP5009 (MS1). | Elkhawaga MA | J Appl Microbiol | 10.1111/jam.13665 | 2018 | Genes, Bacterial, Petroleum/metabolism, Phylogeny, Salinity, Sequence Analysis, DNA, Soil/chemistry, Soil Microbiology, Soil Pollutants/*metabolism, Streptomyces/classification/*metabolism, Surface-Active Agents/chemistry/*isolation & purification/metabolism | Phylogeny |
| Phylogeny | 30741626 | Streptacidiphilus bronchialis sp. nov., a ciprofloxacin-resistant bacterium from a human clinical specimen; reclassification of Streptomyces griseoplanus as Streptacidiphilus griseoplanus comb. nov. and emended description of the genus Streptacidiphilus. | Nouioui I, Klenk HP, Igual JM, Gulvik CA, Lasker BA, McQuiston JR | Int J Syst Evol Microbiol | 10.1099/ijsem.0.003267 | 2019 | Aged, 80 and over, Bacterial Typing Techniques, Base Composition, Bronchoalveolar Lavage Fluid/*microbiology, Ciprofloxacin, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Drug Resistance, Bacterial, Fatty Acids/chemistry, Humans, Male, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Streptomyces/*classification, Streptomycetaceae/*classification/isolation & purification, Tennessee | Pathogenicity |
| Pathogenicity | 32165815 | Potentials of Actinomycetes from Reserved Environments as Antibacterial Agents Against Drug-Resistant Clinical Bacterial Strains. | Ahmed RN, Daniel F, Gbala ID, Sanni A | Ethiop J Health Sci | 10.4314/ejhs.v30i2.13 | 2020 | Actinobacteria/*physiology, Anti-Bacterial Agents/*pharmacology, Cells, Cultured/microbiology, Drug Resistance, Multiple, Bacterial/*drug effects, Methicillin-Resistant Staphylococcus aureus/*drug effects, Nigeria, *Soil Microbiology |
Reference
| @id | authors | catalogue | doi/url | title | journal | pubmed |
|---|---|---|---|---|---|---|
| 9211 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 40009) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-40009 | |||
| 19384 | Wink, J. | http://www.dsmz.de/microorganisms/wink_pdf/DSM40009.pdf | Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
| 67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
| 68368 | Automatically annotated from API 20E | |||||
| 68382 | Automatically annotated from API zym | |||||
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |||
| 84369 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID37095.1 | StrainInfo: A central database for resolving microbial strain identifiers |