Name: Peterkaempfera griseoplana AA-223
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 15274

Type strain:

Species: Peterkaempfera griseoplana

Strain Designation: AA-223

Culture col. no.: DSM 40009, AS 4.1868, ATCC 19766, CBS 504.68, IFO 12779, ISP 5009, NBRC 12779, RIA 1046, JCM 4300, BCRC 13649, CGMCC 4.1868, CGMCC 4.1903, JCM 4582, NCIMB 9811, NRRL B-3064, VKM Ac-1727, VTT E-073013

Strain history: KCC S-0300 <-- CUB 138 <-- ISP 5009 <-- H. D. Tresner AA-223.

NCBI tax ID(s): 66896 (species)

SI-ID 37095

ATCC 19766, IFO 12779, DSM 40009, JCM 4300, CCRC 13649, NCIMB 9811, NRRL B-3064, VKM Ac-1727, JCM 4582, CBS 504.68, NBRC 12779, AS 4.1868, BCRC 13649, NCIB 9811, CGMCC 4.1868, CGMCC 4.1903, VTT E-073013, DSM 4009

Section

Peterkaempfera griseoplana AA-223 is a spore-forming, mesophilic bacterium that builds an aerial mycelium and was isolated from grassland soil.

spore-forming
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Kitasatosporales*
	Family	*Streptomycetaceae*
	Genus	*Peterkaempfera*
	Species	**Peterkaempfera griseoplana**
	Full Scientific Name (LPSN)	Peterkaempfera griseoplana (Backus et al. 1957) Madhaiyan et al. 2022

	Synonym	Streptomyces griseoplanus
	Synonym	Streptacidiphilus griseoplanus

Information on morphological and physiological properties Morphology

[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #69480]	Motility	no Confidence in %90.396

[Ref.: #19384]	Colony color	Pastel yellow
[Ref.: #19384]	Incubation period	10-14 days
[Ref.: #19384]	Cultivation medium used	ISP 2

[Ref.: #19384]	Colony color	Creme
[Ref.: #19384]	Incubation period	10-14 days
[Ref.: #19384]	Cultivation medium used	ISP 5

[Ref.: #19384]	Colony color	Deep orange
[Ref.: #19384]	Incubation period	10-14 days
[Ref.: #19384]	Cultivation medium used	ISP 7

[Ref.: #19384]	Incubation period	10-14 days
[Ref.: #19384]	Cultivation medium used	ISP 3

[Ref.: #19384]	Incubation period	10-14 days
[Ref.: #19384]	Cultivation medium used	ISP 4

[Ref.: #19384]	Incubation period	10-14 days
[Ref.: #19384]	Cultivation medium used	ISP 6

[Ref.: #19384]	Multicellular complex forming ability	yes
[Ref.: #19384]	Multicellular complex name	Aerial Mycelium
[Ref.: #19384]	Multicellular complex color	Creme
[Ref.: #19384]	Medium Name (multicellularity)	ISP 2

[Ref.: #19384]	Multicellular complex forming ability	yes
[Ref.: #19384]	Multicellular complex name	Aerial Mycelium
[Ref.: #19384]	Multicellular complex color	Dusty grey
[Ref.: #19384]	Medium Name (multicellularity)	ISP 3

[Ref.: #19384]	Multicellular complex forming ability	yes
[Ref.: #19384]	Multicellular complex name	Aerial Mycelium
[Ref.: #19384]	Multicellular complex color	Dusty grey
[Ref.: #19384]	Medium Name (multicellularity)	ISP 4

[Ref.: #19384]	Multicellular complex forming ability	yes
[Ref.: #19384]	Multicellular complex name	Aerial Mycelium
[Ref.: #19384]	Multicellular complex color	Creme
[Ref.: #19384]	Medium Name (multicellularity)	ISP 5

[Ref.: #19384]	Multicellular complex forming ability	no
[Ref.: #19384]	Medium Name (multicellularity)	ISP 6

[Ref.: #19384]	Multicellular complex forming ability	yes
[Ref.: #19384]	Multicellular complex name	Aerial Mycelium
[Ref.: #19384]	Multicellular complex color	Creme
[Ref.: #19384]	Medium Name (multicellularity)	ISP 7

Information on culture and growth conditions Culture and growth conditions

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Culture medium

ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

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Culture medium

ISP 2

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Culture medium growth

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Culture medium composition

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Culture medium

ISP 3

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Culture medium growth

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Culture medium composition

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Culture medium

ISP 4

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Culture medium growth

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Culture medium composition

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Culture medium

ISP 5

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Culture medium growth

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Culture medium composition

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Culture medium

ISP 6

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Culture medium growth

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Culture medium composition

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Culture medium

ISP 7

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Culture medium growth

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Culture medium composition

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Culture medium

GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

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		Temperature
[Ref.: #9211]	growth	28 °C
[Ref.: #19384]	optimum	28 °C
[Ref.: #67770]	growth	28 °C

[Ref.: #9211]	Temperature range	mesophilic
[Ref.: #19384]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #69481]	Ability of spore formation	yes Confidence in %100
[Ref.: #69480]	Ability of spore formation	yes Confidence in %100

[Ref.: #9211]

Name of produced compound

alazopeptin

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #19384]		NaCl		growth	0	%

[Ref.: #67770]

Observation

quinones: MK9(H₆)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68368]	arginine ChEBI	-	hydrolysis	* from API 20E
[Ref.: #68368]	citrate ChEBI	-	assimilation	* from API 20E
[Ref.: #68368]	gelatin ChEBI	+	hydrolysis	* from API 20E
[Ref.: #68368]	lysine ChEBI	-	degradation	* from API 20E
[Ref.: #68368]	ornithine ChEBI	-	degradation	* from API 20E
[Ref.: #68368]	tryptophan ChEBI	-	energy source	* from API 20E
[Ref.: #68368]	urea ChEBI	+	hydrolysis	* from API 20E

	Metabolite	Production
[Ref.: #67770]	alazopeptin	yes
[Ref.: #68368]	acetoin ChEBI	yes	* from API 20E
[Ref.: #68368]	hydrogen sulfide ChEBI	no	* from API 20E
[Ref.: #68368]	indole ChEBI	no	* from API 20E

	Physiological tests	Metabolite	Voges-Proskauer-test	Indole test
[Ref.: #68368]		acetoin ChEBI	+		* from API 20E
[Ref.: #68368]		indole ChEBI		-	* from API 20E

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	+	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	+	3.2.1.24	* from API zym
[Ref.: #68368]	arginine dihydrolase	-	3.5.3.6	* from API 20E
[Ref.: #68368]	beta-galactosidase	+	3.2.1.23	* from API 20E, API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #68368]	gelatinase	+		* from API 20E
[Ref.: #68368]	lysine decarboxylase	-	4.1.1.18	* from API 20E
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #68368]	ornithine decarboxylase	-	4.1.1.17	* from API 20E
[Ref.: #68368]	tryptophan deaminase	-	4.1.99.1	* from API 20E
[Ref.: #68368]	urease	+	3.5.1.5	* from API 20E
	Only first 10 entries are displayed. Click here to see all.

API® 20E:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	beta-GalactosidaseONPG	Arginine dihydrolaseADH (Arg)	Lysine decarboxylaseLDC (Lys)	Ornithine decarboxylaseODC	Citrate utilizationCIT	H2S productionH2S	Urease/urea hydrolysisURE	Tryptophan deaminaseTDA (Trp)	Indole productionIND	Acetoin production (Voges Proskauer test)VP	Gelatinase/gelatin hydrolysisGEL	Fermentation/oxidation of D-glucoseGLU	Fermentation/oxidation of D-mannitolMAN	Fermentation/oxidation of inositolINO	Fermentation/oxidation of sorbitolSor	Fermentation/oxidation of L-rhamnoseRHA	Fermentation/oxidation of sucroseSAC	Fermentation/oxidation of melibioseMEL	Fermentation/oxidation of amygdalinAMY	Fermentation/oxidation of L-arabinoseARA	Cytochrome oxidaseOX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	Oxidative catabolisation of D-glucoseOF-O	Fermentation of D-glucoseOF-F
[Ref.: #19384]	250	+	-	-	-	-	-	+	-	-	+	+	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #19384]	339	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	+	+	+	+	-	+	-	+	+	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #9211]	Sample type/isolated from	grassland soil
[Ref.: #9211]	Geographic location (country and/or sea, region)	Iowa
[Ref.: #9211]	Country	USA
[Ref.: #9211]	Country ISO 3 Code	USA
[Ref.: #9211]	Continent	North America

[Ref.: #67770]	Sample type/isolated from	Grassland soil near Williamsberg
[Ref.: #67770]	Geographic location (country and/or sea, region)	IA
[Ref.: #67770]	Country	USA
[Ref.: #67770]	Country ISO 3 Code	USA
[Ref.: #67770]	Continent	North America
* marker position based on {}

Isolation sources categories

#Environmental	#Terrestrial	#Grassland
#Environmental	#Terrestrial	#Soil

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #9211]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #19384]	Biosafety level	1	Risk group (German classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	Streptomyces pratensis strain AS 4.1868 16S ribosomal RNA gene, partial sequence	AY999894	1401	ENA	1169025 tax ID	*
[Ref.: #20218]	Streptomyces pratensis strain CGMCC 4.1868 clone 1 16S ribosomal RNA gene, complete sequence	JQ824033	1477	ENA	1169025 tax ID	*
[Ref.: #20218]	Streptomyces pratensis strain CGMCC 4.1868 clone 2 16S ribosomal RNA gene, complete sequence	JQ824034	1477	ENA	1169025 tax ID	*
[Ref.: #20218]	Streptomyces pratensis strain CGMCC 4.1868 clone 3 16S ribosomal RNA gene, complete sequence	JQ824035	1478	ENA	1169025 tax ID	*
[Ref.: #20218]	Streptomyces pratensis strain CGMCC 4.1868 clone 4 16S ribosomal RNA gene, complete sequence	JQ824036	1477	ENA	1169025 tax ID	*
[Ref.: #20218]	Streptomyces griseoplanus gene for 16S rRNA, partial sequence	AB122751	564	ENA	66896 tax ID	*
[Ref.: #20218]	Streptomyces griseoplanus strain NRRL_ISP 5009 clone 1 16S ribosomal RNA gene, partial sequence	JQ806225	1483	ENA	66896 tax ID	*
[Ref.: #20218]	Streptomyces griseoplanus strain NRRL_ISP 5009 clone 2 16S ribosomal RNA gene, partial sequence	JQ806226	1480	ENA	66896 tax ID	*
[Ref.: #20218]	Streptomyces griseoplanus strain NRRL_ISP 5009 clone 3 16S ribosomal RNA gene, partial sequence	JQ806227	1480	ENA	66896 tax ID	*
[Ref.: #20218]	Streptomyces griseoplanus strain NRRL_ISP 5009 clone 4 16S ribosomal RNA gene, partial sequence	JQ806228	1480	ENA	66896 tax ID	*
[Ref.: #20218]	Streptomyces griseoplanus gene for 16S ribosomal RNA, partial sequence, strain: JCM 4300	D44048	120	ENA	66896 tax ID	*
[Ref.: #20218]	Streptomyces griseoplanus gene for 16S rRNA, partial sequence, strain: NBRC 12779	AB184138	1467	ENA	66896 tax ID	*
[Ref.: #20218]	Streptomyces griseoplanus strain NRRL B-3064 clone 1 16S ribosomal RNA gene, partial sequence	JQ806222	1480	ENA	66896 tax ID	*
[Ref.: #20218]	Streptomyces griseoplanus strain NRRL B-3064 clone 3 16S ribosomal RNA gene, partial sequence	JQ806223	1480	ENA	66896 tax ID	*
[Ref.: #20218]	Streptomyces griseoplanus strain NRRL B-3064 clone 4 16S ribosomal RNA gene, partial sequence	JQ806224	1480	ENA	66896 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Peterkaempfera griseoplana NRRL B-3064	GCA_001418575	scaffold	GenBank	66896 tax ID

[Ref.: #67770]

GC-content

72.5 mol%

genome sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Peterkaempfera griseoplana NRRL B-3064 NCBI: GCA_001418575.1
[Ref.: #69481]	spore-forming	yes	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Peterkaempfera griseoplana NRRL B-3064 NCBI: GCA_001418575.1
[Ref.: #69480]	flagellated	no	97.904	no
[Ref.: #69480]	gram-positive	yes	90.294	no
[Ref.: #69480]	anaerobic	no	99.095	no
[Ref.: #69480]	aerobic	yes	89.435	no
[Ref.: #69480]	halophile	no	94.013	yes
[Ref.: #69480]	spore-forming	yes	95.523	no
[Ref.: #69480]	glucose-util	yes	91.051	no
[Ref.: #69480]	thermophile	no	98.654	no
[Ref.: #69480]	motile	no	95.226	no
[Ref.: #69480]	glucose-ferment	no	88.526	no

Availability in culture collections External links

[Ref.: #9211]

Culture collection no.

DSM 40009, AS 4.1868, ATCC 19766, CBS 504.68, IFO 12779, ISP 5009, NBRC 12779, RIA 1046, JCM 4300, BCRC 13649, CGMCC 4.1868, CGMCC 4.1903, JCM 4582, NCIMB 9811, NRRL B-3064, VKM Ac-1727, VTT E-073013

[Ref.: #84369]

SI-ID 37095

Literature:

Topic	Title	Authors	Journal	DOI	Year
Pathogenicity	Potentials of Actinomycetes from Reserved Environments as Antibacterial Agents Against Drug-Resistant Clinical Bacterial Strains.	Ahmed RN, Daniel F, Gbala ID, Sanni A	Ethiop J Health Sci	10.4314/ejhs.v30i2.13	2020	*
Phylogeny	Streptacidiphilus bronchialis sp. nov., a ciprofloxacin-resistant bacterium from a human clinical specimen; reclassification of Streptomyces griseoplanus as Streptacidiphilus griseoplanus comb. nov. and emended description of the genus Streptacidiphilus.	Nouioui I, Klenk HP, Igual JM, Gulvik CA, Lasker BA, McQuiston JR	Int J Syst Evol Microbiol	10.1099/ijsem.0.003267	2019	*
Metabolism	Optimization and characterization of biosurfactant from Streptomyces griseoplanus NRRL-ISP5009 (MS1).	Elkhawaga MA	J Appl Microbiol	10.1111/jam.13665	2018	*
Phylogeny	Allostreptomyces psammosilenae gen. nov., sp. nov., an endophytic actinobacterium isolated from the roots of Psammosilene tunicoides and emended description of the family Streptomycetaceae [Waksman and Henrici (1943)AL] emend. Rainey et al. 1997, emend. Kim et al. 2003, emend. Zhi et al. 2009.	Huang MJ, Rao MPN, Salam N, Xiao M, Huang HQ, Li WJ	Int J Syst Evol Microbiol	10.1099/ijsem.0.001617	2017	*
Phylogeny	Classification of Streptomyces phylogroup pratensis (Doroghazi and Buckley, 2010) based on genetic and phenotypic evidence, and proposal of Streptomyces pratensis sp. nov.	Rong X, Doroghazi JR, Cheng K, Zhang L, Buckley DH, Huang Y	Syst Appl Microbiol	10.1016/j.syapm.2013.03.010	2013	*

References

#9211

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 40009

#19384

Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68368

Automatically annotated from API 20E .

#68382

Automatically annotated from API zym .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#84369

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID37095.1 )

* These data were automatically processed and therefore are not curated

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Peterkaempfera griseoplana NRRL B-3064

Peterkaempfera griseoplana NRRL B-3064

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