Strain identifier

BacDive ID: 15266

Type strain: Yes

Species: Streptomyces murinus

Strain Designation: 1068

Strain history: KCC S-0276 <-- S. T. Williams A-107 <-- ISP 5191 <-- Meiji Seika Kaisha, Ltd.; 1068.

NCBI tax ID(s): 146922 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 9253

BacDive-ID: 15266

DSM-Number: 40191

keywords: genome sequence, 16S sequence, Bacteria, spore-forming, mesophilic, Gram-positive, antibiotic compound production

description: Streptomyces murinus 1068 is a spore-forming, mesophilic, Gram-positive bacterium that produces antibiotic compounds and was isolated from soil.

NCBI tax id

  • NCBI tax id: 146922
  • Matching level: species

strain history

@refhistory
9253<- E.B. Shirling, ISP <- M. Oda, Meiji Seika Co., 1068
67770KCC S-0276 <-- S. T. Williams A-107 <-- ISP 5191 <-- Meiji Seika Kaisha, Ltd.; 1068.

doi: 10.13145/bacdive15266.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces murinus
  • full scientific name: Streptomyces murinus Frommer 1959 (Approved Lists 1980)

  • synonyms

    @refsynonym
    20215Streptomyces costaricanus
    20215Streptomyces griseofuscus
    20215Streptomyces phaeogriseichromatogenes

@ref: 9253

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces griseofuscus

full scientific name: Streptomyces griseofuscus Sakamoto et al. 1962 emend. Nouioui et al. 2018

strain designation: 1068

type strain: yes

Morphology

cell morphology

  • @ref: 69480
  • gram stain: positive
  • confidence: 100

multimedia

  • @ref: 9253
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_40191.jpg
  • caption: Medium 987 28°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
9253GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
9253ISP2 MEDIUM (DSMZ Medium 987)yeshttps://mediadive.dsmz.de/medium/987Name: ISP 2 MEDIUM (DSMZ Medium 987) Composition: Agar 20.0 g/l Malt extract 10.0 g/l Dextrose 4.0 g/l Yeast extract 4.0 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
18555positiveoptimum28mesophilic
9253positivegrowth28mesophilic
67770positivegrowth28mesophilic

Physiology and metabolism

spore formation

@refspore formationconfidence
69481yes100
69480yes100

compound production

@refcompound
9253bundlin A
9253bundlin B
9253moldicidin A
9253pentamycin
9253bundlin A, B
20216Bundlin A
20216Bundlin B
20216Moldicin A
20216Pentamycin
20216Glucose isomerase
67770Bundlins A and B and moldcidin B;

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1855517234glucose+
1855522599arabinose+
1855517992sucrose-
1855518222xylose-
1855529864mannitol+
1855528757fructose+
1855526546rhamnose-
1855516634raffinose-
1855562968cellulose+
1855517268myo-inositol-
683685291gelatin+hydrolysis
6836827897tryptophan-energy source
6836816199urea+hydrolysis
6836816947citrate+assimilation
6836818257ornithine+degradation
6836825094lysine+degradation
6836829016arginine+hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
6777031639fungichrominyes
6836815688acetoinno
6836835581indoleno
6836816136hydrogen sulfideno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
6836815688acetoin-
6836835581indole-

enzymes

@refvalueecactivity
67770glucose isomerase5.3.1.18
68368gelatinase+
68368tryptophan deaminase4.1.99.1-
68368urease3.5.1.5+
68368ornithine decarboxylase4.1.1.17+
68368lysine decarboxylase4.1.1.18+
68368arginine dihydrolase3.5.3.6+
68368beta-galactosidase3.2.1.23+
68382alkaline phosphatase3.1.3.1+
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase3.4.11.1+
68382valine arylamidase+
68382cystine arylamidase3.4.11.3+
68382trypsin3.4.21.4-
68382alpha-chymotrypsin3.4.21.1-
68382acid phosphatase3.1.3.2+
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase3.2.1.22-
68382beta-galactosidase3.2.1.23+
68382beta-glucuronidase3.2.1.31-
68382alpha-glucosidase3.2.1.20+
68382beta-glucosidase3.2.1.21+
68382N-acetyl-beta-glucosaminidase3.2.1.52+
68382alpha-mannosidase3.2.1.24+
68382alpha-fucosidase3.2.1.51-

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
18555+++-+++--++-+-++++-

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGEL
18555+++++-+---+

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentgeographic location
9253soilJapanJPNAsia
67770SoilJapanJPNAsiaFukushima Pref.

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

taxonmaps

  • @ref: 69479
  • File name: preview.99_3145.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15;96_102;97_224;98_2413;99_3145&stattab=map
  • Last taxonomy: Streptomyces
  • 16S sequence: AY207605
  • Sequence Identity:
  • Total samples: 462
  • soil counts: 384
  • aquatic counts: 10
  • animal counts: 13
  • plant counts: 55

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
185551German classification
92531Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Streptomyces griseofuscus strain IFO 12870 clone ITS25 16S-23S ribosomal RNA intergenic spacer, partial sequenceAY296915247ena146922
20218Streptomyces griseofuscus strain IFO 12870 clone ITS8 16S-23S ribosomal RNA intergenic spacer, partial sequenceAY296916265ena146922
20218Streptomyces griseofuscus strain IFO 12870 clone ITS9 16S-23S ribosomal RNA intergenic spacer, partial sequenceAY296917280ena146922
20218Streptomyces rochei gene for 16S ribosomal RNA, partial sequence, strain: JCM 4276D44041120ena1928
20218Streptomyces griseofuscus 16S ribosomal RNA gene, partial sequenceAY2076051491ena146922
20218Streptomyces griseofuscus strain KCTC 9879 16S ribosomal RNA gene, partial sequenceAY9998091419ena146922
20218Streptomyces griseofuscus gene for 16S rRNA, partial sequence, strain: NBRC 12870AB1842061476ena146922

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Streptomyces griseofuscus DSM 40191GCA_014673495completencbi146922
66792Streptomyces griseofuscus strain DSM 40191146922.15completepatric146922
67770Streptomyces griseofuscus NRRL B-5429GCA_000718315contigncbi146922

GC content

  • @ref: 67770
  • GC-content: 71.7
  • method: genome sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
flagellatedno98.086no
gram-positiveyes90.302no
anaerobicno98.757no
aerobicyes90.859no
halophileno93.978no
spore-formingyes95.957no
glucose-utilyes88.309no
thermophileno96.354yes
motileno94.251no
glucose-fermentno90.428no

External links

@ref: 9253

culture collection no.: DSM 40191, ATCC 23916, CBS 837.68, IFO 12870, ISP 5191, JCM 4276, JCM 4641, NBRC 12870, RIA 1145, BCRC 10483, CECT 3307, CGMCC 4.1962, IMET 42068, KACC 20083, KCTC 9879, NCIMB 9821, NRRL B-5429, VKM Ac-1707

straininfo link

  • @ref: 84361
  • straininfo: 389375

literature

Pubmed-IDtitleauthorsjournalDOIyeartopicmeshtopic2
33214305High-Quality Sequencing, Assembly, and Annotation of the Streptomyces griseofuscus DSM 40191 Genome.Gren T, Jorgensen TS, Whitford CM, Weber TMicrobiol Resour Announc10.1128/MRA.01100-202020
34526549Characterization and engineering of Streptomyces griseofuscus DSM 40191 as a potential host for heterologous expression of biosynthetic gene clusters.Gren T, Whitford CM, Mohite OS, Jorgensen TS, Kontou EE, Nielsen JB, Lee SY, Weber TSci Rep10.1038/s41598-021-97571-22021GeneticsComputational Biology/methods, Data Mining, *Gene Expression, *Genetic Engineering/methods, Genome, Bacterial, Genomics/methods, Multigene Family, Phenotype, Plasmids/genetics, Recombinant Proteins/*biosynthesis/*genetics/isolation & purification, Secondary Metabolism, Streptomyces/*genetics/metabolismPhenotype

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
9253Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 40191)https://www.dsmz.de/collection/catalogue/details/culture/DSM-40191
18555Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM40191.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20216Curators of the HKIhttp://www.leibniz-hki.de/de/Collection Description Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. Hans-Knöll-Institut (HKI)
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
84361Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID389375.1StrainInfo: A central database for resolving microbial strain identifiers