Strain identifier

BacDive ID: 1521

Type strain: Yes

Species: Lysinibacillus fusiformis

Strain Designation: AMNH 732

Strain history: CIP <- 2009, J.F. Charles, Inst. Pasteur, Paris, France <- 2001, B. Papierok, Inst. Pasteur, Paris, France <- 1998, M.M. Lecadet, Inst. Pasteur, Paris, France <- 1992, H. de Barjac, Inst. Pasteur, Paris, France <- 1978, A.A. Yousten: strain ATCC 7055

NCBI tax ID(s): 28031 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 1163

BacDive-ID: 1521

DSM-Number: 2898

keywords: genome sequence, 16S sequence, Bacteria, spore-forming, mesophilic, Gram-positive, motile, rod-shaped

description: Lysinibacillus fusiformis AMNH 732 is a spore-forming, mesophilic, Gram-positive bacterium that was isolated from Soil.

NCBI tax id

  • NCBI tax id: 28031
  • Matching level: species

strain history

@refhistory
1163<- Schering AG <- ATCC (Bacillus sphaericus) <- N.R. Smith, 350 <- AMNH, 732
67770NRRL NRS-350 <-- N. R. Smith NRS-350 <-- AMNH 732.
122731CIP <- 2009, J.F. Charles, Inst. Pasteur, Paris, France <- 2001, B. Papierok, Inst. Pasteur, Paris, France <- 1998, M.M. Lecadet, Inst. Pasteur, Paris, France <- 1992, H. de Barjac, Inst. Pasteur, Paris, France <- 1978, A.A. Yousten: strain ATCC 7055

doi: 10.13145/bacdive1521.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Caryophanaceae
  • genus: Lysinibacillus
  • species: Lysinibacillus fusiformis
  • full scientific name: Lysinibacillus fusiformis (Priest et al. 1989 ex Meyer and Gottheil 1901) Ahmed et al. 2007

  • synonyms

    • @ref: 20215
    • synonym: Bacillus fusiformis

@ref: 1163

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Caryophanaceae

genus: Lysinibacillus

species: Lysinibacillus fusiformis

full scientific name: Lysinibacillus fusiformis (Priest et al. 1989) Ahmed et al. 2007

strain designation: AMNH 732

type strain: yes

Morphology

cell morphology

  • @ref: 23066
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: yes

Culture and growth conditions

culture medium

  • @ref: 1163
  • name: NUTRIENT AGAR (DSMZ Medium 1)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/1
  • composition: Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
1163positivegrowth30mesophilic
67770positivegrowth30mesophilic

Physiology and metabolism

spore formation

  • @ref: 23066
  • spore description: ellipsoidal or spherical,terminal,in a swollen sporangium
  • type of spore: endospore
  • spore formation: yes

compound production

  • @ref: 1163
  • compound: steroids

murein

  • @ref: 23066
  • murein short key: A11.31
  • type: A4alpha L-Lys-D-Asp

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2306617632nitrate-reduction
12273117234glucose-fermentation
1227312759inuline-fermentation
12273117716lactose-fermentation
12273117632nitrate-reduction
12273118222xylose-fermentation

metabolite production

@refChebi-IDmetaboliteproduction
2306635581indoleno
2306616136hydrogen sulfideno

metabolite tests

  • @ref: 122731
  • Chebi-ID: 17234
  • metabolite: glucose
  • methylred-test: -

enzymes

@refvalueactivityec
23066beta-galactosidase-3.2.1.23
23066catalase+1.11.1.6
23066cytochrome oxidase+1.9.3.1
122731lecithinase-
122731urease+3.5.1.5

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentisolation date
67770Soil
122731United States of AmericaUSANorth America1978

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
11631Risk group (German classification)
1227311Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Bacillus fusiformis clone DS-8_F 16S ribosomal RNA gene, partial sequence; 16S-23S internal transcribed spacer, complete sequence; and 23S ribosomal RNA gene, partial sequenceAF478081538ena28031
20218Bacillus fusiformis clone DS-6_f 16S ribosomal RNA gene, partial sequence; 16S-23S internal transcribed spacer, complete sequence; and 23S ribosomal RNA gene, partial sequenceAF478082467ena28031
20218Bacillus fusiformis clone DS-5N_f 16S ribosomal RNA gene, partial sequence; 16S-23S internal transcribed spacer, complete sequence; tRNA-Ile and tRNA-Ala genes, complete sequence; and 23S ribosomal RNA gene, partial sequenceAF478083579ena28031
20218Bacillus fusiformis 16S rRNA gene, strain DSM 2898TAJ3100831515ena28031
20218Bacillus fusiformis complete 16S ribosomal RNA geneL140131492ena28031
20218Bacillus fusiformis (DSM 2898) ribosomal RNA small subunitM774861430ena28031
1163Bacillus fusiformis 16S ribosomal RNA gene, partial sequenceAF1695371412ena28031
67770Lysinibacillus fusiformis gene for 16S rRNA, partial sequenceAB2454231474ena28031
67770Bacillus fusiformis gene for 16S rRNA, partial sequenceAB2717431477ena28031

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Lysinibacillus fusiformis strain ATCC 705528031.24wgspatric28031
66792Lysinibacillus fusiformis strain NBRC 1571728031.55wgspatric28031
66792Lysinibacillus fusiformis ATCC 70552816332540draftimg28031
67770Lysinibacillus fusiformis NBRC 15717GCA_006540205contigncbi28031
67770Lysinibacillus fusiformis NRRL NRS-350GCA_003367495contigncbi28031
67770Lysinibacillus fusiformis ATCC 7055GCA_003049525contigncbi28031

GC content

@refGC-contentmethod
116336.0
6777035-36thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileyes88.684no
gram-positiveyes91.325yes
anaerobicno97.624no
halophileno90.61no
spore-formingyes96.742no
glucose-utilyes83.071no
flagellatedyes87.799no
aerobicyes91.549no
thermophileno99.95no
glucose-fermentno88.915no

External links

@ref: 1163

culture collection no.: DSM 2898, ATCC 7055, JCM 31033, NRS 350, JCM 12229, BCRC 15907, CCUG 28888, IFO 15717, IFO 3528, KCTC 3454, LMG 9816, NBRC 15717, NBRC 3528, NRRL NRS-350, VTT E-011945, CRBIP3.1597

straininfo link

  • @ref: 71167
  • straininfo: 92143

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny17473269Proposal of Lysinibacillus boronitolerans gen. nov. sp. nov., and transfer of Bacillus fusiformis to Lysinibacillus fusiformis comb. nov. and Bacillus sphaericus to Lysinibacillus sphaericus comb. nov.Ahmed I, Yokota A, Yamazoe A, Fujiwara TInt J Syst Evol Microbiol10.1099/ijs.0.63867-02007Bacillaceae/chemistry/*classification/genetics/metabolism, Bacterial Typing Techniques, Base Composition, Boron/metabolism, Cell Wall/chemistry, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, Genes, rRNA, Hydrogen-Ion Concentration, Molecular Sequence Data, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phylogeny, Quinones/analysis, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sequence Homology, Nucleic Acid, Sodium Chloride/metabolism, *Soil Microbiology, TemperatureMetabolism
Phylogeny19502328Lysinibacillus parviboronicapiens sp. nov., a low-boron-containing bacterium isolated from soil.Miwa H, Ahmed I, Yokota A, Fujiwara TInt J Syst Evol Microbiol10.1099/ijs.0.65455-02009Bacterial Typing Techniques, Base Composition, Boron/*metabolism, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Genotype, Gram-Positive Asporogenous Rods/*classification/genetics/isolation & purification/physiology, Molecular Sequence Data, Peptidoglycan/analysis, Phenotype, Phylogeny, Polymerase Chain Reaction/methods, Polymorphism, Restriction Fragment Length, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Species Specificity, TurkeyGenetics
Phylogeny22140163Description of Lysinibacillus sinduriensis sp. nov., and transfer of Bacillus massiliensis and Bacillus odysseyi to the genus Lysinibacillus as Lysinibacillus massiliensis comb. nov. and Lysinibacillus odysseyi comb. nov. with emended description of the genus Lysinibacillus.Jung MY, Kim JS, Paek WK, Styrak I, Park IS, Sin Y, Paek J, Park KA, Kim H, Kim HL, Chang YHInt J Syst Evol Microbiol10.1099/ijs.0.033837-02011Bacillaceae/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/analysis, Geologic Sediments/microbiology, Molecular Sequence Data, Nucleic Acid Hybridization, Peptidoglycan/*analysis, Phospholipids/analysis, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sequence Analysis, DNA, *Soil MicrobiologyGenetics
Phylogeny22934997Isolation and molecular identification of landfill bacteria capable of growing on di-(2-ethylhexyl) phthalate and deteriorating PVC materials.Latorre I, Hwang S, Montalvo-Rodriguez RJ Environ Sci Health A Tox Hazard Subst Environ Eng10.1080/10934529.2012.7075492012Bacteria/*classification/genetics/*isolation & purification/metabolism, Biodegradation, Environmental, DNA, Bacterial/genetics, Diethylhexyl Phthalate/*metabolism, Endocrine Disruptors/*metabolism, Phylogeny, Polymerase Chain Reaction, Polyvinyl Chloride/*metabolism, RNA, Ribosomal, 16S/genetics, Refuse Disposal, Sequence Analysis, RNA, Water Pollutants, Chemical/*metabolismMetabolism
Phylogeny32248284Screening and Optimization of Newly Isolated Thermotolerant Lysinibacillus fusiformis Strain SK for Protease and Antifungal Activity.Khadka S, Adhikari S, Thapa A, Panday R, Adhikari M, Sapkota S, Regmi RS, Adhikari NP, Proshad R, Koirala NCurr Microbiol10.1007/s00284-020-01976-72020*Antibiosis, Aspergillus, Aspergillus flavus, Bacillaceae/classification/*enzymology/isolation & purification, Bacterial Typing Techniques, DNA, Bacterial/genetics, Nepal, Peptide Hydrolases/*metabolism, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil MicrobiologyEnzymology

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
1163Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 2898)https://www.dsmz.de/collection/catalogue/details/culture/DSM-2898
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
23066Iftikhar Ahmed,Akira Yokota,Atsushi Yamazoe,Toru Fujiwara10.1099/ijs.0.63867-0Proposal of Lysinibacillus boronitolerans gen. nov. sp. nov., and transfer of Bacillus fusiformis to Lysinibacillus fusiformis comb. nov. and Bacillus sphaericus to Lysinibacillus sphaericus comb. nov.IJSEM 57: 1117-1125 200717473269
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
71167Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID92143.1StrainInfo: A central database for resolving microbial strain identifiers
122731Curators of the CIPCollection of Institut Pasteur (CRBIP3.1597)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CRBIP3.1597