Strain identifier

BacDive ID: 15145

Type strain: No

Species: Streptomyces griseus

Strain Designation: Duché

Strain history: KCC S-0472 <-- IFO 13037 <-- SAJ <-- ISP 5322 <-- CBS 420.34 <-- J. Duché.

NCBI tax ID(s): 67295 (species)

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General

@ref: 9460

BacDive-ID: 15145

DSM-Number: 40322

keywords: genome sequence, 16S sequence, Bacteria, mesophilic

description: Streptomyces griseus Duché is a mesophilic bacterium that builds an aerial mycelium and was isolated from manure.

NCBI tax id

  • NCBI tax id: 67295
  • Matching level: species

strain history

@refhistory
9460<- E.B. Shirling, ISP <- CBS, Duché
67770KCC S-0472 <-- IFO 13037 <-- SAJ <-- ISP 5322 <-- CBS 420.34 <-- J. Duché.

doi: 10.13145/bacdive15145.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces griseus
  • full scientific name: Streptomyces griseus (Krainsky 1914) Waksman and Henrici 1948 (Approved Lists 1980)

  • synonyms

    @refsynonym
    20215Actinomyces globisporus subsp. flavofuscus
    20215Actinomyces acrimycini
    20215Actinomyces setonii
    20215Actinomyces fimicarius
    20215Streptomyces setonii
    20215Streptomyces fimicarius
    20215Streptomyces globisporus subsp. flavofuscus
    20215Streptomyces baarnensis
    20215Streptomyces acrimycini
    20215Actinomyces griseus
    20215Streptomyces erumpens
    20215Streptomyces argenteolus
    20215Streptomyces flavofuscus
    20215Streptomyces caviscabies

@ref: 9460

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces griseus subsp. griseus

full scientific name: Streptomyces griseus subsp. griseus (Krainsky 1914) Pridham 1970

strain designation: Duché

type strain: no

Morphology

colony morphology

@refcolony colorincubation periodmedium used
19810Sand yellow10-14 daysISP 2
19810Light ivory10-14 daysISP 3
19810Ivory10-14 daysISP 4
19810Ivory10-14 daysISP 5
19810Sand yellow10-14 daysISP 6
19810Brown beige10-14 daysISP 7

multicellular morphology

@refforms multicellular complexcomplex namemedium namecomplex color
19810yesAerial MyceliumISP 2
19810yesAerial MyceliumISP 3Oyster white
19810yesAerial MyceliumISP 4Oyster white
19810yesAerial MyceliumISP 5
19810noISP 6
19810noISP 7

multimedia

  • @ref: 9460
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_40322.jpg
  • caption: Medium 65 28°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
9460ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium84.pdf
19810ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19810ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19810ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19810ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19810ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19810ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
9460GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
19810positiveoptimum28mesophilic
9460positivegrowth28mesophilic
67770positivegrowth28mesophilic

Physiology and metabolism

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1981062968cellulose+
1981016634raffinose+/-
1981026546rhamnose+
1981028757fructose+
1981029864mannitol+
1981017268myo-inositol+/-
1981018222xylose+/-
1981017992sucrose+/-
1981022599arabinose+
1981017234glucose+
683685291gelatin+hydrolysis
6836827897tryptophan-energy source
6836816199urea+hydrolysis
6836816947citrate+assimilation
6836818257ornithine+degradation
6836825094lysine+degradation
6836829016arginine+hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
6836815688acetoinno
6836835581indoleno
6836816136hydrogen sulfideno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
6836815688acetoin-
6836835581indole-

enzymes

@refvalueactivityec
68368gelatinase+
68368tryptophan deaminase-4.1.99.1
68368urease+3.5.1.5
68368ornithine decarboxylase+4.1.1.17
68368lysine decarboxylase+4.1.1.18
68368arginine dihydrolase+3.5.3.6
68368beta-galactosidase+3.2.1.23
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)+
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase+3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin+3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase+3.2.1.24
68382alpha-fucosidase+3.2.1.51

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
19810+++++++++++-+-+++++

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGEL
19810+++++-+---+

Isolation, sampling and environmental information

isolation

  • @ref: 9460
  • sample type: manure

isolation source categories

  • Cat1: #Host Body Product
  • Cat2: #Gastrointestinal tract
  • Cat3: #Feces (Stool)

taxonmaps

  • @ref: 69479
  • File name: preview.99_135.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15;96_102;97_109;98_120;99_135&stattab=map
  • Last taxonomy: Streptomyces
  • 16S sequence: AY999784
  • Sequence Identity:
  • Total samples: 4030
  • soil counts: 2441
  • aquatic counts: 286
  • animal counts: 1009
  • plant counts: 294

Safety information

risk assessment

@refbiosafety levelbiosafety level commentpathogenicity plant
198101Risk group (German classification)
94601Risk group (German classification)yes

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
9460Streptomyces fimicarius strain ISP 5322 16S ribosomal RNA gene, partial sequenceAY9997841496ena67295
9460Streptomyces fimicarius gene for 16S rRNA, partial sequence, strain: NBRC 13037AB1842691474ena67295

Genome sequences

  • @ref: 67770
  • description: Streptomyces fimicarius NRRL ISP-5322
  • accession: GCA_002154345
  • assembly level: scaffold
  • database: ncbi
  • NCBI tax ID: 67295

GC content

  • @ref: 67770
  • GC-content: 74
  • method: fluorimetric

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
flagellatedno97.724no
gram-positiveyes90.963no
anaerobicno99.332no
halophileno89.287no
spore-formingyes94.104no
glucose-utilyes87.928no
aerobicyes93.441no
thermophileno98.936no
motileno92.867no
glucose-fermentno84.637no

External links

@ref: 9460

culture collection no.: DSM 40322, ATCC 25449, CBS 420.34, CBS 682.69, IFO 13037, ISP 5322, NBRC 13037, RIA 1229, JCM 4472, BCRC 12245, CGMCC 4.1629, KCCM 40680, NRRL ISP-5322, VKM Ac-1724, VTT E-011974

straininfo link

  • @ref: 84249
  • straininfo: 386975

literature

  • topic: Phylogeny
  • Pubmed-ID: 34889725
  • title: Streptomyces silvae sp. nov., isolated from forest soil.
  • authors: Besaury L, Martinet L, Muhle E, Clermont D, Remond C
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.005147
  • year: 2021
  • mesh: Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Forests, France, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, *Streptomyces/classification/isolation & purification, Vitamin K 2/analogs & derivatives/chemistry
  • topic2: Transcriptome

Reference

@idauthorscataloguedoi/urltitle
9460Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 40322)https://www.dsmz.de/collection/catalogue/details/culture/DSM-40322
19810Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM40322.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
84249Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID386975.1StrainInfo: A central database for resolving microbial strain identifiers