Strain identifier
BacDive ID: 15123
Type strain: 
Species: Streptomyces diastaticus
Strain history: KCC S-0128 <-- M. Mayama <-- ATCC 3315 <-- S. A. Waksman.
NCBI tax ID(s): 1956 (species)
version 8.1 (current version)
General
@ref: 9599
BacDive-ID: 15123
DSM-Number: 40496
keywords: genome sequence, 16S sequence, Bacteria, spore-forming, mesophilic, Gram-positive
description: Streptomyces diastaticus DSM 40496 is a spore-forming, mesophilic, Gram-positive bacterium that builds an aerial mycelium.
NCBI tax id
- NCBI tax id: 1956
- Matching level: species
strain history
| @ref | history |
|---|---|
| 9599 | <- E.B. Shirling, ISP <- ATCC |
| 67770 | KCC S-0128 <-- M. Mayama <-- ATCC 3315 <-- S. A. Waksman. |
doi: 10.13145/bacdive15123.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Kitasatosporales
- family: Streptomycetaceae
- genus: Streptomyces
- species: Streptomyces diastaticus
- full scientific name: Streptomyces diastaticus (Krainsky 1914) Waksman and Henrici 1948 (Approved Lists 1980)
synonyms
@ref synonym 20215 Actinomyces diastaticus 20215 Actinomyces rutgersensis 20215 Actinomyces gougeroti 20215 Streptomyces gougerotii 20215 Streptomyces rutgersensis
@ref: 9599
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Streptomycetaceae
genus: Streptomyces
species: Streptomyces diastaticus subsp. diastaticus
full scientific name: Streptomyces diastaticus subsp. diastaticus (Krainsky 1914) Pridham et al. 1958
type strain: yes
Morphology
cell morphology
- @ref: 69480
- gram stain: positive
- confidence: 100
colony morphology
| @ref | colony color | incubation period | medium used |
|---|---|---|---|
| 19466 | Yellow | 10-14 days | ISP 2 |
| 19466 | Beige | 10-14 days | ISP 3 |
| 19466 | Beige | 10-14 days | ISP 4 |
| 19466 | Beige | 10-14 days | ISP 5 |
multicellular morphology
| @ref | forms multicellular complex | complex name | complex color | medium name |
|---|---|---|---|---|
| 19466 | yes | Aerial Mycelium | Yellow | ISP 2 |
| 19466 | yes | Aerial Mycelium | White | ISP 3 |
| 19466 | yes | Aerial Mycelium | Beige | ISP 4 |
| 19466 | yes | Aerial Mycelium | White | ISP 5 |
| 19466 | no | ISP 6 | ||
| 19466 | no | ISP 7 |
Culture and growth conditions
culture medium
| @ref | name | growth | link | composition |
|---|---|---|---|---|
| 9599 | GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) | yes | https://mediadive.dsmz.de/medium/65 | Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water |
| 19466 | ISP 2 | yes | Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes | |
| 19466 | ISP 3 | yes | Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms: | |
| 19466 | ISP 4 | yes | Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml | |
| 19466 | ISP 5 | yes | Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes | |
| 9599 | ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84) | yes | https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium84.pdf |
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 19466 | positive | optimum | 30 | mesophilic |
| 9599 | positive | growth | 28 | mesophilic |
| 67770 | positive | growth | 28 | mesophilic |
Physiology and metabolism
spore formation
| @ref | spore description | type of spore | spore formation | confidence |
|---|---|---|---|---|
| 19466 | Formation of spore chains (rectiflixibilis), spore surface smooth | spore | yes | |
| 69481 | yes | 100 | ||
| 69480 | yes | 100 |
halophily
- @ref: 19466
- salt: NaCl
- growth: positive
- tested relation: maximum
- concentration: 5 %
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity |
|---|---|---|---|
| 19466 | 16634 | raffinose | - |
| 19466 | 26546 | rhamnose | - |
| 19466 | 28757 | fructose | + |
| 19466 | 29864 | mannitol | + |
| 19466 | 17268 | myo-inositol | - |
| 19466 | 18222 | xylose | + |
| 19466 | 17992 | sucrose | + |
| 19466 | 22599 | arabinose | + |
| 19466 | 17234 | glucose | + |
Safety information
risk assessment
| @ref | biosafety level | biosafety level comment |
|---|---|---|
| 9599 | 1 | Risk group (German classification) |
| 19466 | 1 | Risk group (German classification) |
Sequence information
16S sequences
| @ref | description | accession | length | database | NCBI tax ID |
|---|---|---|---|---|---|
| 20218 | Streptomyces diastaticus partial 16S rRNA | X53161 | 1354 | ena | 1956 |
| 20218 | Streptomyces diastaticus subsp. diastaticus gene for 16S ribosomal RNA, partial sequence, strain: JCM 4745 | D44329 | 120 | ena | 68040 |
| 20218 | Streptomyces diastaticus subsp. diastaticus gene for 16S rRNA, partial sequence, strain: NBRC 13412 | AB184386 | 1446 | ena | 68040 |
| 20218 | Streptomyces diastaticus subsp. diastaticus gene for 16S rRNA, partial sequence, strain: NBRC 3714 | AB184785 | 1478 | ena | 68040 |
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Streptomyces diastaticus subsp. diastaticus JCM 4128 | GCA_014648935 | scaffold | ncbi | 68040 |
| 66792 | Streptomyces diastaticus subsp. diastaticus strain JCM 4128 | 68040.4 | wgs | patric | 68040 |
| 66792 | Streptomyces diastaticus subsp. diastaticus strain NBRC 13412 | 68040.3 | wgs | patric | 68040 |
| 67770 | Streptomyces diastaticus subsp. diastaticus NBRC 13412 | GCA_011170125 | contig | ncbi | 68040 |
GC content
- @ref: 67770
- GC-content: 73.4
- method: genome sequence analysis
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| spore-forming | yes | 100 | no |
| flagellated | no | 97.463 | no |
| gram-positive | yes | 91.576 | no |
| anaerobic | no | 99.433 | no |
| aerobic | yes | 93.686 | no |
| halophile | no | 91.5 | no |
| spore-forming | yes | 94.919 | yes |
| thermophile | no | 98.137 | no |
| glucose-util | yes | 89.643 | yes |
| motile | no | 95.885 | no |
| glucose-ferment | no | 87.824 | no |
External links
@ref: 9599
culture collection no.: DSM 40496, ATCC 3315, CBS 713.72, IFO 13412, IFO 3714, ISP 5496, JCM 4128, JCM 4745, NBRC 13412, NBRC 3714, NRRL B-1270, RIA 1373, BCRC 15168, CBS 126.20, CCM 3167, CCUG 11116, CGMCC 4.1420, IMET 40274, LMG 19322, MTCC 1394, NRRL B-1241, RIA 104, VKM Ac-723
straininfo link
- @ref: 84228
- straininfo: 14106
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 24002610 | Streptomyces polyrhachii sp. nov., a novel actinomycete isolated from an edible Chinese black ant (Polyrhachis vicina Roger). | Yu C, Liu C, Wang X, Zhao J, Yang L, Gao R, Zhang Y, Xiang W | Antonie Van Leeuwenhoek | 10.1007/s10482-013-0021-3 | 2013 | Animals, Bacterial Typing Techniques, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Hymenoptera/*microbiology, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Streptomyces/*classification/genetics/*isolation & purification/metabolism | Genetics |
| Phylogeny | 32644916 | Reclassification of Streptomyces diastaticus subsp. ardesiacus, Streptomyces gougerotii and Streptomyces rutgersensis. | Komaki H, Tamura T | Int J Syst Evol Microbiol | 10.1099/ijsem.0.004287 | 2020 | Bacterial Typing Techniques, DNA, Bacterial/genetics, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Streptomyces/*classification | Transcriptome |
Reference
| @id | authors | catalogue | doi/url | title | journal | pubmed |
|---|---|---|---|---|---|---|
| 9599 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 40496) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-40496 | |||
| 19466 | Wink, J. | http://www.dsmz.de/microorganisms/wink_pdf/DSM40496.pdf | Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |||
| 84228 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID14106.1 | StrainInfo: A central database for resolving microbial strain identifiers |