Strain identifier

BacDive ID: 15091

Type strain: Yes

Species: Streptomyces chryseus

Strain history: KCC S-0737 <-- IFO 13377 <-- SAJ <-- ISP 5420 <-- N. A. Krassilnikov 1007-B.

NCBI tax ID(s): 68186 (species)

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General

@ref: 9529

BacDive-ID: 15091

DSM-Number: 40420

keywords: genome sequence, 16S sequence, Bacteria, spore-forming, mesophilic

description: Streptomyces chryseus DSM 40420 is a spore-forming, mesophilic bacterium that builds an aerial mycelium.

NCBI tax id

  • NCBI tax id: 68186
  • Matching level: species

strain history

@refhistory
9529<- E.B. Shirling, ISP <- N.A. Krassilnikov, INMI
67770KCC S-0737 <-- IFO 13377 <-- SAJ <-- ISP 5420 <-- N. A. Krassilnikov 1007-B.

doi: 10.13145/bacdive15091.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces chryseus
  • full scientific name: Streptomyces chryseus (Krassilnikov et al. 1965) Pridham 1970 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Actinomyces chryseus

@ref: 9529

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces chryseus

full scientific name: Streptomyces chryseus (Krassilnikov et al. 1965) Pridham 1970

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram stain
69480no93.313
69480100positive

colony morphology

@refcolony colorincubation periodmedium used
19451Yellow orange10-14 daysISP 2
19451Rape yellow10-14 daysISP 3
19451Beige10-14 daysISP 4
19451Beige10-14 daysISP 5
19451Beige10-14 daysISP 6
19451Lemon yellow10-14 daysISP 7

multicellular morphology

@refforms multicellular complexcomplex namecomplex colormedium name
19451yesAerial MyceliumCreamISP 2
19451yesAerial MyceliumCreamISP 3
19451yesAerial MyceliumCreamISP 4
19451noISP 5
19451noISP 6
19451noISP 7

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
9529GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
19451ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19451ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19451ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19451ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19451ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19451ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
9529ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium84.pdf

culture temp

@refgrowthtypetemperaturerange
19451positiveoptimum28mesophilic
9529positivegrowth28mesophilic
67770positivegrowth28mesophilic

Physiology and metabolism

spore formation

@refspore formationconfidence
69481yes100
69480yes100

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
683685291gelatin+hydrolysis
6836827897tryptophan-energy source
6836816199urea+hydrolysis
6836816947citrate+assimilation
6836818257ornithine+degradation
6836825094lysine+degradation
6836829016arginine+hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
6836815688acetoinyes
6836835581indoleno
6836816136hydrogen sulfideno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
6836815688acetoin+
6836835581indole-

enzymes

@refvalueactivityec
68368gelatinase+
68368tryptophan deaminase-4.1.99.1
68368urease+3.5.1.5
68368ornithine decarboxylase+4.1.1.17
68368lysine decarboxylase+4.1.1.18
68368arginine dihydrolase+3.5.3.6
68368beta-galactosidase+3.2.1.23
68382lipase (C 14)-
68382alpha-galactosidase-3.2.1.22
68382beta-glucuronidase-3.2.1.31
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refLipasealpha- Galactosidasebeta- Glucuronidasealpha- Mannosidasealpha- Fucosidase
19451-----

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGEL
19451+++++-+--++

Isolation, sampling and environmental information

taxonmaps

  • @ref: 69479
  • File name: preview.99_834.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15;96_102;97_109;98_662;99_834&stattab=map
  • Last taxonomy: Streptomyces
  • 16S sequence: AY999787
  • Sequence Identity:
  • Total samples: 1611
  • soil counts: 953
  • aquatic counts: 126
  • animal counts: 103
  • plant counts: 429

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
95291Risk group (German classification)
194511Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Streptomyces chryseus gene for 16S ribosomal RNA, partial sequence, strain: JCM 4737D44321120ena68186
20218Streptomyces chryseus gene for 16S rRNA, partial sequence, strain: NBRC 13377AB1848761477ena68186
20218Streptomyces chryseus strain NRRL B-12347 16S ribosomal RNA gene, partial sequenceAY9997871494ena68186

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Streptomyces chryseus JCM 4737GCA_014650755contigncbi68186
66792Streptomyces chryseus strain DSM 4042068186.4wgspatric68186
66792Streptomyces chryseus strain JCM 473768186.5wgspatric68186
67770Streptomyces chryseus DSM 40420GCA_005981935contigncbi68186

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
gram-positiveyes91.316no
anaerobicno99.06no
halophileno94.056no
spore-formingyes94.451no
glucose-utilyes89.404no
thermophileno96.162yes
flagellatedno98.419no
aerobicyes91.449no
glucose-fermentno91.05no
motileno95.077no

External links

@ref: 9529

culture collection no.: DSM 40420, ATCC 19829, CBS 678.72, IFO 13377, INMI 1007 B, ISP 5420, NBRC 13377, RIA 1338, JCM 4737, BCRC 16236, CGMCC 4.1694, NCIMB 10041, NRRL B-12347, VKM Ac-200

straininfo link

  • @ref: 84197
  • straininfo: 37114

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Genetics11092967Structure of anionic carbohydrate-containing cell wall polymers in several representatives of the order actinomycetales.Kozlova YI, Streshinskaya GM, Shashkov AS, Evtushenko LI, Gavrish EY, Naumova IBBiochemistry (Mosc)BCM651014322000Actinomycetales/*chemistry/classification, Biopolymers/*chemistry, Carbohydrate Sequence, Carbohydrates/chemistry, Cell Wall/*chemistry, Magnetic Resonance Spectroscopy, Molecular Sequence Data, Streptomyces/chemistry, Teichoic Acids/*chemistryPhylogeny
Phylogeny19567563Streptomyces hypolithicus sp. nov., isolated from an Antarctic hypolith community.Le Roes-Hill M, Rohland J, Meyers PR, Cowan DA, Burton SGInt J Syst Evol Microbiol10.1099/ijs.0.007971-02009Antarctic Regions, Bacterial Typing Techniques, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Microscopy, Electron, Scanning, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Streptomyces/*classification/genetics/*isolation & purification/physiologyGenetics
Phylogeny31204968Streptomyces altiplanensis sp. nov., an alkalitolerant species isolated from Chilean Altiplano soil, and emended description of Streptomyces chryseus (Krasil'nikov et al. 1965) Pridham 1970.Cortes-Albayay C, Dorador C, Schumann P, Schniete JK, Herron P, Andrews B, Asenjo J, Nouioui IInt J Syst Evol Microbiol10.1099/ijsem.0.0035252019Alkalies, Altitude, Bacterial Typing Techniques, Base Composition, Chile, DNA, Bacterial/genetics, *Desert Climate, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Hydrogen-Ion Concentration, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Streptomyces/*classification/isolation & purificationTranscriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
9529Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 40420)https://www.dsmz.de/collection/catalogue/details/culture/DSM-40420
19451Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM40420.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
84197Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID37114.1StrainInfo: A central database for resolving microbial strain identifiers